UniProtKB - P42327 (ADH2_GEOSE)
Protein
Alcohol dehydrogenase
Gene
adh
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
Active with primary alcohols, including methanol.
Catalytic activityi
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Activity regulationi
The rate-limiting step is NADH release. Catabolite repression.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 38 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 61 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 92 | Zinc 2By similarity | 1 | |
Metal bindingi | 95 | Zinc 2By similarity | 1 | |
Metal bindingi | 98 | Zinc 2By similarity | 1 | |
Metal bindingi | 106 | Zinc 2By similarity | 1 | |
Metal bindingi | 148 | Zinc 1; catalyticBy similarity | 1 | |
Binding sitei | 195 | NADBy similarity | 1 | |
Binding sitei | 200 | NADBy similarity | 1 | |
Binding sitei | 331 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 172 – 177 | NADBy similarity | 6 | |
Nucleotide bindingi | 260 – 262 | NADBy similarity | 3 |
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:adh |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160737 | 1 – 339 | Alcohol dehydrogenaseAdd BLAST | 339 |
Proteomic databases
PRIDEi | P42327 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P42327 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the zinc-containing alcohol dehydrogenase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
i Sequence
Sequence statusi: Complete.
P42327-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAAVVNEFK KALEIKEVER PKLEEGEVLV KIEACGVCHT DLHAAHGDWP
60 70 80 90 100
IKPKLPLIPG HEGVGIVVEV AKGVKSIKVG DRVGIPWLYS ACGECEYCLT
110 120 130 140 150
GQETLCPHQL NGGYSVDGGY AEYCKAPADY VAKIPDNLDP VEVAPILCAG
160 170 180 190 200
VTTYKALKVS GARPGEWVAI YGIGGLGHIA LQYAKAMGLN VVAVDISDEK
210 220 230 240 250
SKLAKDLGAD IAINGLKEDP VKAIHDQVGG VHAAISVAVN KKAFEQAYQS
260 270 280 290 300
VKRGGTLVVV GLPNADLPIP IFDTVLNGVS VKGSIVGTRK DMQEALDFAA
310 320 330
RGKVRPIVET AELEEINEVF ERMEKGKING RIVLKLKED
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25544 Genomic DNA Translation: CAA80989.1 |
PIRi | S47643 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25544 Genomic DNA Translation: CAA80989.1 |
PIRi | S47643 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6IQD | X-ray | 2.84 | A/B/C/D/E/F/G/H | 1-339 | [»] | |
SMRi | P42327 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P42327 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADH2_GEOSE | |
Accessioni | P42327Primary (citable) accession number: P42327 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | October 7, 2020 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families