Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 133 (11 Dec 2019)
Sequence version 1 (01 Nov 1995)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Alpha-ketoglutaric semialdehyde dehydrogenase

Gene

gucD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the NAD(P)+-dependent oxidation of alpha-ketoglutaric semialdehyde (alphaKGSA) to alpha-ketoglutarate. Prefers NADP+ to NAD+ as a cosubstrate. In vitro, can also use various aldehydes.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 2.75 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NAD+). kcat is 1.12 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NADP+). kcat is 1.29 sec(-1) with propionaldehyde as substrate (in the presence of NADP+). kcat is 1.35 sec(-1) with butylaldehyde as substrate (in the presence of NADP+). kcat is 2.10 sec(-1) with valeraldehyde as substrate (in the presence of NADP+). kcat is 1.47 sec(-1) with hexylaldehyde as substrate (in the presence of NADP+). kcat is 0.87 sec(-1) with heptalaldehyde as substrate (in the presence of NADP+). kcat is 0.97 sec(-1) with octylaldehyde as substrate (in the presence of NADP+).1 Publication
  1. KM=10.4 µM for alpha-ketoglutaric semialdehyde (in the presence of NAD+, at 25 degrees Celsius and pH 7.2)1 Publication
  2. KM=2.8 µM for alpha-ketoglutaric semialdehyde (in the presence of NADP+, at 25 degrees Celsius and pH 7.2)1 Publication
  3. KM=1460 µM for propionaldehyde (in the presence of NADP+)1 Publication
  4. KM=170 µM for butylaldehyde (in the presence of NADP+)1 Publication
  5. KM=145 µM for valeraldehyde (in the presence of NADP+)1 Publication
  6. KM=81.4 µM for hexylaldehyde (in the presence of NADP+)1 Publication
  7. KM=39.3 µM for heptalaldehyde (in the presence of NADP+)1 Publication
  8. KM=35.2 µM for octylaldehyde (in the presence of NADP+)1 Publication
  9. KM=556.9 µM for NAD+ (at 25 degrees Celsius and pH 7.2)1 Publication
  10. KM=94 µM for NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  1. Vmax=1.14 µmol/min/mg enzyme for the oxidation of alpha-ketoglutaric semialdehyde with NAD+ (at 25 degrees Celsius and pH 7.2)1 Publication
  2. Vmax=1.05 µmol/min/mg enzyme for the oxidation of alpha-ketoglutaric semialdehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  3. Vmax=0.53 µmol/min/mg enzyme for the oxidation of propionaldehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  4. Vmax=1.02 µmol/min/mg enzyme for the oxidation of butylaldehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  5. Vmax=1.34 µmol/min/mg enzyme for the oxidation of valeraldehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  6. Vmax=1.08 µmol/min/mg enzyme for the oxidation of hexylaldehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  7. Vmax=0.90 µmol/min/mg enzyme for the oxidation of heptalaldehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication
  8. Vmax=0.87 µmol/min/mg enzyme for the oxidation of octylaldehyde with NADP+ (at 25 degrees Celsius and pH 7.2)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei255Proton acceptorBy similarity1
Active sitei289NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi233 – 238NAD(P)By similarity6

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU02470-MONOMER

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P42236

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha-ketoglutaric semialdehyde dehydrogenase1 Publication (EC:1.2.1.261 Publication)
Short name:
alphaKGSA dehydrogenase1 Publication
Alternative name(s):
2,5-dioxovalerate dehydrogenaseCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:gucDImported
Synonyms:ycbD
Ordered Locus Names:BSU02470
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

No effect on vanillin degradation.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000564441 – 488Alpha-ketoglutaric semialdehyde dehydrogenaseAdd BLAST488

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P42236

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P42236

PRoteomics IDEntifications database

More...
PRIDEi
P42236

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer.

1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU02470

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P42236

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105C26 Bacteria
COG1012 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000271511

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P42236

KEGG Orthology (KO)

More...
KOi
K22187

Identification of Orthologs from Complete Genome Data

More...
OMAi
WTRMLVH

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P42236

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.309.10, 1 hit
3.40.605.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00171 Aldedh, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53720 SSF53720, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P42236-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVITEQNTY LNFINGEWVK SQSGDMVKVE NPADVNDIVG YVQNSTAEDV
60 70 80 90 100
ERAVTAANEA KTAWRKLTGA ERGQYLYKTA DIMEQRLEEI AACATREMGK
110 120 130 140 150
TLPEAKGETA RGIAILRYYA GEGMRKTGDV IPSTDKDALM FTTRVPLGVV
160 170 180 190 200
GVISPWNFPV AIPIWKMAPA LVYGNTVVIK PATETAVTCA KIIACFEEAG
210 220 230 240 250
LPAGVINLVT GPGSVVGQGL AEHDGVNAVT FTGSNQVGKI IGQAALARGA
260 270 280 290 300
KYQLEMGGKN PVIVADDADL EAAAEAVITG AFRSTGQKCT ATSRVIVQSG
310 320 330 340 350
IYERFKEKLL QRTKDITIGD SLKEDVWMGP IASKNQLDNC LSYIEKGKQE
360 370 380 390 400
GASLLIGGEK LENGKYQNGY YVQPAIFDNV TSEMTIAQEE IFGPVIALIK
410 420 430 440 450
VDSIEEALNI ANDVKFGLSA SIFTENIGRM LSFIDEIDAG LVRINAESAG
460 470 480
VELQAPFGGM KQSSSHSREQ GEAAKDFFTA IKTVFVKP
Length:488
Mass (Da):52,415
Last modified:November 1, 1995 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9C37B32EF51D59B2
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D30808 Genomic DNA Translation: BAA06468.1
AL009126 Genomic DNA Translation: CAB12041.1

Protein sequence database of the Protein Information Resource

More...
PIRi
G69752

NCBI Reference Sequences

More...
RefSeqi
NP_388129.1, NC_000964.3
WP_003246263.1, NZ_JNCM01000030.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB12041; CAB12041; BSU02470

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
938406

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU02470

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.254

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30808 Genomic DNA Translation: BAA06468.1
AL009126 Genomic DNA Translation: CAB12041.1
PIRiG69752
RefSeqiNP_388129.1, NC_000964.3
WP_003246263.1, NZ_JNCM01000030.1

3D structure databases

SMRiP42236
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU02470

Proteomic databases

jPOSTiP42236
PaxDbiP42236
PRIDEiP42236

Genome annotation databases

EnsemblBacteriaiCAB12041; CAB12041; BSU02470
GeneIDi938406
KEGGibsu:BSU02470
PATRICifig|224308.179.peg.254

Phylogenomic databases

eggNOGiENOG4105C26 Bacteria
COG1012 LUCA
HOGENOMiHOG000271511
InParanoidiP42236
KOiK22187
OMAiWTRMLVH
PhylomeDBiP42236

Enzyme and pathway databases

BioCyciBSUB:BSU02470-MONOMER
SABIO-RKiP42236

Family and domain databases

Gene3Di3.40.309.10, 1 hit
3.40.605.10, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiKGSDH_BACSU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P42236
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: December 11, 2019
This is version 133 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again