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UniProtKB - P42224 (STAT1_HUMAN)

Protein

Signal transducer and activator of transcription 1-alpha/beta

Gene

STAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.7 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAntiviral defense, Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1059683 Interleukin-6 signaling
R-HSA-1169408 ISG15 antiviral mechanism
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-1839117 Signaling by cytosolic FGFR1 fusion mutants
R-HSA-186763 Downstream signal transduction
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-877300 Interferon gamma signaling
R-HSA-877312 Regulation of IFNG signaling
R-HSA-8854691 Interleukin-20 family signaling
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8984722 Interleukin-35 Signalling
R-HSA-8985947 Interleukin-9 signaling
R-HSA-9013508 NOTCH3 Intracellular Domain Regulates Transcription
R-HSA-9020956 Interleukin-27 signaling
R-HSA-9020958 Interleukin-21 signaling
R-HSA-909733 Interferon alpha/beta signaling
R-HSA-912694 Regulation of IFNA signaling
R-HSA-982772 Growth hormone receptor signaling
SignaLinkiP42224
SIGNORiP42224

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducer and activator of transcription 1-alpha/beta
Alternative name(s):
Transcription factor ISGF-3 components p91/p84
Gene namesi
Name:STAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115415.18
HGNCiHGNC:11362 STAT1
MIMi600555 gene
neXtProtiNX_P42224

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Immunodeficiency 31B (IMD31B)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by susceptibility to severe mycobacterial and viral infections. Affected individuals can develop disseminated infections and die of viral illness.
See also OMIM:613796
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_065815201K → N in IMD31B; not deleterious in terms of most STAT1 functions; causes abnormal splicing out of exon 8 from most mRNAs thereby decreasing protein levels by approximately 70%. 1 PublicationCorresponds to variant dbSNP:rs587776870EnsemblClinVar.1
Natural variantiVAR_018265600L → P in IMD31B; found in an infant who died of a viral-like illness associated with complete STAT1 deficiency. 1 PublicationCorresponds to variant dbSNP:rs137852678EnsemblClinVar.1
Natural variantiVAR_075500701Y → C in IMD31B; disrupts transactivation activity in response to IFNG. 1 Publication1
Immunodeficiency 31A (IMD31A)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Mendelian susceptibility to mycobacterial disease, a rare condition caused by impairment of interferon-gamma mediated immunity. It is characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine, environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. Clinical outcome severity depends on the degree of impairment of interferon-gamma mediated immunity. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. IMD31A has low penetrance, and affected individuals have relatively mild disease and good prognosis. IMD31A confers a predisposition to mycobacterial infections only, with no increased susceptibility to viral infections.
See also OMIM:614892
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_065816320E → Q in IMD31A; affects the DNA-binding activity of the protein. 1 PublicationCorresponds to variant dbSNP:rs137852680EnsemblClinVar.1
Natural variantiVAR_065817463Q → H in IMD31A; affects the DNA-binding activity of the protein. 1 PublicationCorresponds to variant dbSNP:rs137852679EnsemblClinVar.1
Natural variantiVAR_068713637K → E in IMD31A; affects both phosphorylation and DNA-binding activity; results in impaired STAT1-mediated cellular response to IFN-gamma and interleukin-27. 1 PublicationCorresponds to variant dbSNP:rs587777705EnsemblClinVar.1
Natural variantiVAR_068714673K → R in IMD31A; impairs tyrosine phosphorylation; results in impaired STAT1-mediated cellular response to IFN-gamma and interleukin-27. 1 PublicationCorresponds to variant dbSNP:rs587777704EnsemblClinVar.1
Natural variantiVAR_018266706L → S in IMD31A; loss of GAF and ISGF3 activation; impairs the nuclear accumulation of GAF but not of ISGF3 in heterozygous cells stimulated by IFNs; affects phosphorylation of the protein. 2 PublicationsCorresponds to variant dbSNP:rs137852677EnsemblClinVar.1
Immunodeficiency 31C (IMD31C)5 Publications
The disease is caused by mutations affecting the gene represented in this entry. STAT1 mutations in patients with autosomal dominant candidiasis lead to defective responses of type 1 and type 17 helper T-cells, characterized by reduced production of interferon-alpha, interleukin-17, and interleukin-22. These cytokines are crucial for the antifungal defense of skin and mucosa (PubMed:21714643).1 Publication
Disease descriptionA primary immunodeficiency disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
See also OMIM:614162
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_065934165D → G in IMD31C; gain of function mutation associated with increased STAT1 phosphorylation due to impaired nuclear dephosphorylation. 2 PublicationsCorresponds to variant dbSNP:rs387906764EnsemblClinVar.1
Natural variantiVAR_065935165D → H in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906767EnsemblClinVar.1
Natural variantiVAR_065936170Y → N in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906766EnsemblClinVar.1
Natural variantiVAR_065937174C → R in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906763EnsemblClinVar.1
Natural variantiVAR_075494179N → K in IMD31C; gain of function; increases transactivation activity in response to IFNG. 1 PublicationCorresponds to variant dbSNP:rs587777628EnsemblClinVar.1
Natural variantiVAR_065938202M → I in IMD31C. 1 Publication1
Natural variantiVAR_065939202M → V in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906762EnsemblClinVar.1
Natural variantiVAR_065940267A → V in IMD31C. 2 PublicationsCorresponds to variant dbSNP:rs387906759EnsemblClinVar.1
Natural variantiVAR_065941271Q → P in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906768EnsemblClinVar.1
Natural variantiVAR_065942274R → Q in IMD31C; gain of function; increases STAT1 phosphorylation due to impaired nuclear dephosphorylation; increases transactivation activity in response to IFNG. 2 PublicationsCorresponds to variant dbSNP:rs387906760EnsemblClinVar.1
Natural variantiVAR_065943274R → W in IMD31C; gain of function; increases phosphorylation in response to IFNG, IFNA and IL27 due to a loss of dephosphorylation. 3 PublicationsCorresponds to variant dbSNP:rs387906758EnsemblClinVar.1
Natural variantiVAR_075495278K → E in IMD31C; gain of function; increases phosphorylation in response to IFNG and IFNA due to a loss of dephosphorylation. 1 PublicationCorresponds to variant dbSNP:rs863223398EnsemblClinVar.1
Natural variantiVAR_075496285Q → R in IMD31C; gain of function; increases transactivation activity in response to IFNG. 1 PublicationCorresponds to variant dbSNP:rs587777629EnsemblClinVar.1
Natural variantiVAR_065944286K → I in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906761EnsemblClinVar.1
Natural variantiVAR_065945288T → A in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906765EnsemblClinVar.1
Natural variantiVAR_075497298K → N in IMD31C; gain of function; increases basal STAT1 phosphorylation levels which are 10-20 fold higher than controls after IFNG stimulation. 1 Publication1
Natural variantiVAR_075498384G → D in IMD31C; gain of function; increases phosphorylation in response to IFNG and IFNA due to a loss of dephosphorylation. 1 PublicationCorresponds to variant dbSNP:rs796065052EnsemblClinVar.1
Natural variantiVAR_075499385T → M in IMD31C; gain of function; increases phosphorylation in response to IFNG, IFNA and IL27 due to a loss of dephosphorylation. 2 PublicationsCorresponds to variant dbSNP:rs587777630EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110K → R: Sumoylated. 1 Publication1
Mutagenesisi114K → A: No effect on IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. 1 Publication1
Mutagenesisi175K → A: No effect on IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. 1 Publication1
Mutagenesisi296K → A: No effect on IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. 1 Publication1
Mutagenesisi366K → A: No effect on IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. 1 Publication1
Mutagenesisi525K → A: Strongly reduced IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. Does not affect ability to homodimerize. 1 Publication1
Mutagenesisi636 – 637KK → AA: No effect on IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. 1 Publication2
Mutagenesisi656 – 658AEN → CEC: Enhances STAT1 nuclear translocation and interferon (IFN)-stimulated gene (ISG) expression in response to IFN-beta stimulation. Reduces viral load in infected cultured cells. 1 Publication3
Mutagenesisi657E → Q: Loss of ADP-ribosylation and increased Tyr-701 phosphorylation; when associated with Q-705. 1 Publication1
Mutagenesisi665K → A: No effect on IFN-alpha-induced STAT1 phosphorylation and nuclear translocation. 1 Publication1
Mutagenesisi701Y → E: Not phosphorylated at S-708 upon IFNB induction. 2 Publications1
Mutagenesisi701Y → F: No effect on basal sumoylation. Enhances sumoylation in the presence of MAPK stimulation. Phosphorylated at S-708 upon IFNB induction. 2 Publications1
Mutagenesisi703K → R: Abolishes sumoylation by SUMO1. Increased IFN-gamma-mediated transactivation. 2 Publications1
Mutagenesisi705E → Q: Loss of ADP-ribosylation and increased Tyr-701 phosphorylation; when associated with Q-657. 1 Publication1
Mutagenesisi708S → A: Phosphorylated at Y-701 upon IFNB induction. 1 Publication1
Mutagenesisi708S → D: Not phosphorylated at Y-701 upon IFNB induction. 1 Publication1
Mutagenesisi727S → A: Decreased transcriptional activation. No effect on basal sumoylation. No enhancement of sumoylation on MAPK stimulation. No PRKCD-induced apoptosis. Upon IFNB induction, phosphorylated at Y-701 but not at S-708. 3 Publications1
Mutagenesisi727S → D: No change in enhancement of MAPK-induced sumoylation. Basal interaction with PIAS1. Interaction with PIAS1 increased on MAPK stimulation. 3 Publications1
Mutagenesisi727S → E: No change in enhancement of MAPK-induced sumoylation. 3 Publications1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi6772
MalaCardsiSTAT1
MIMi613796 phenotype
614162 phenotype
614892 phenotype
OpenTargetsiENSG00000115415
Orphaneti391487 Autoimmune enteropathy and endocrinopathy-susceptibility to chronic infections syndrome
319595 Mendelian susceptibility to mycobacterial diseases due to partial STAT1 deficiency
391311 Susceptibility to viral and mycobacterial infections
PharmGKBiPA36183

Chemistry databases

ChEMBLiCHEMBL6101

Polymorphism and mutation databases

BioMutaiSTAT1
DMDMi2507413

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001824102 – 750Signal transducer and activator of transcription 1-alpha/betaAdd BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei114N6-methyllysine1 Publication1
Modified residuei175N6-methyllysine1 Publication1
Modified residuei296N6-methyllysine1 Publication1
Modified residuei366N6-methyllysine1 Publication1
Modified residuei525N6-methyllysine1 Publication1
Modified residuei637N6-methyllysine1 Publication1
Modified residuei657ADP-ribosyl glutamic acid; by PARP141 Publication1
Modified residuei665N6-methyllysine1 Publication1
Modified residuei701Phosphotyrosine; by JAK1, JAK2 or TYK28 Publications1
Cross-linki703Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki703Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei705ADP-ribosyl glutamic acid; by PARP141 Publication1
Modified residuei708Phosphoserine; by IKKE1 Publication1
Modified residuei727Phosphoserine; by MAPK14Combined sources6 Publications1
Modified residuei745Phosphoserine; by IKKEBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus. Growth hormone (GH) activates STAT1 signaling only via JAK2. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents. Phosphorylated on tyrosine residues when PTK2/FAK1 is activated; most likely this is catalyzed by a SRC family kinase. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling. Upon viral infection or IFN induction, phosphorylation on Ser-708 occurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent. Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701.10 Publications
Sumoylated with SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.5 Publications
ISGylated.1 Publication
Mono-ADP-ribosylated at Glu-657 and Glu-705 by PARP14; ADP-ribosylation prevents phosphorylation at Tyr-701.1 Publication
Monomethylated at Lys-525 by SETD2; monomethylation is necessary for phosphorylation at Tyr-701, translocation into the nucleus and activation of the antiviral defense.1 Publication

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP42224
MaxQBiP42224
PaxDbiP42224
PeptideAtlasiP42224
PRIDEiP42224
ProteomicsDBi55491
55492 [P42224-2]

PTM databases

CarbonylDBiP42224
iPTMnetiP42224
PhosphoSitePlusiP42224
SwissPalmiP42224

Miscellaneous databases

PMAP-CutDBiP42224

Expressioni

Gene expression databases

BgeeiENSG00000115415 Expressed in 234 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_STAT1
ExpressionAtlasiP42224 baseline and differential
GenevisibleiP42224 HS

Organism-specific databases

HPAiCAB004049
HPA000931
HPA000982

Interactioni

Subunit structurei

Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation (PubMed:8605877, PubMed:28753426). Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation (PubMed:8605877). The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9 (By similarity). Interacts (phosphorylated at Ser-727) with PIAS1 (dimethylated on arginine); the interaction results in release of STAT1 from its target gene (PubMed:9724754, PubMed:17897103, PubMed:19136629). Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466' (PubMed:9121453). Interacts with IFNAR2 (PubMed:9121453). Found in a complex with NMI and CREBBP/CBP (PubMed:9989503). Interacts with NMI which is required for CREBBP/CBP recruitment to the complex (PubMed:9989503). Interacts with PTK2/FAK1 (PubMed:11278462). Interacts with SRC (By similarity). Interacts with ERBB4 (phosphorylated) (PubMed:18721752). Interacts with PARP9 and DTX3L independently of IFN-beta or IFN-gamma-mediated STAT1 'Tyr-701' phosphorylation (PubMed:26479788). Interacts with histone acetyltransferase EP300/p300 in response to INF-gamma stimulation (PubMed:26479788). Interacts with OTOP1 (By similarity).By similarity10 Publications
(Microbial infection) Interacts with Sendai virus C', C, Y1 and Y2 proteins, preventing activation of ISRE and GAS promoter.
(Microbial infection) Interacts with Nipah virus P, V and W proteins preventing activation of ISRE and GAS promoter.
(Microbial infection) Interacts with Rabies virus phosphoprotein preventing activation of ISRE and GAS promoter.2 Publications
(Microbial infection) Interacts with HCV core protein; the interaction results in STAT1 degradation.1 Publication
(Microbial infection) Interacts with ebolavirus protein VP24.1 Publication

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi112649, 163 interactors
CORUMiP42224
DIPiDIP-46140N
IntActiP42224, 99 interactors
MINTiP42224
STRINGi9606.ENSP00000354394

Chemistry databases

BindingDBiP42224

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00962
ProteinModelPortaliP42224
SMRiP42224
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42224

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini573 – 670SH2PROSITE-ProRule annotationAdd BLAST98

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili136 – 3171 PublicationAdd BLAST182

Sequence similaritiesi

Belongs to the transcription factor STAT family.Curated

Keywords - Domaini

Coiled coil, SH2 domain

Phylogenomic databases

eggNOGiKOG3667 Eukaryota
ENOG410XPN8 LUCA
GeneTreeiENSGT00760000119236
HOVERGENiHBG071022
InParanoidiP42224
KOiK11220
OMAiAKWSQLS
OrthoDBiEOG091G03O3
PhylomeDBiP42224
TreeFamiTF318648

Family and domain databases

CDDicd10372 SH2_STAT1, 1 hit
Gene3Di1.10.532.10, 1 hit
1.10.8.1200, 1 hit
2.60.40.630, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR008967 p53-like_TF_DNA-bd
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR001217 STAT
IPR038295 STAT1_C_sf
IPR035859 STAT1_SH2
IPR022752 STAT1_TAZ2-bd_C
IPR036535 STAT_N_sf
IPR013800 STAT_TF_alpha
IPR015988 STAT_TF_coiled-coil
IPR013801 STAT_TF_DNA-bd
IPR012345 STAT_TF_DNA-bd_N
IPR013799 STAT_TF_prot_interaction
PANTHERiPTHR11801 PTHR11801, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF12162 STAT1_TAZ2bind, 1 hit
PF01017 STAT_alpha, 1 hit
PF02864 STAT_bind, 1 hit
PF02865 STAT_int, 1 hit
SMARTiView protein in SMART
SM00964 STAT_int, 1 hit
SUPFAMiSSF47655 SSF47655, 1 hit
SSF48092 SSF48092, 1 hit
SSF49417 SSF49417, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform Alpha (identifier: P42224-1) [UniParc]FASTAAdd to basket
Also known as: p91

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV 
        60         70         80         90        100
SFATIRFHDL LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ 
       110        120        130        140        150
MSMIIYSCLK EERKILENAQ RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK 
       160        170        180        190        200
DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR EHETNGVAKS DQKQEQLLLK 
       210        220        230        240        250
KMYLMLDNKR KEVVHKIIEL LNVTELTQNA LINDELVEWK RRQQSACIGG 
       260        270        280        290        300
PPNACLDQLQ NWFTIVAESL QQVRQQLKKL EELEQKYTYE HDPITKNKQV 
       310        320        330        340        350
LWDRTFSLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKLRLLVK 
       360        370        380        390        400
LQELNYNLKV KVLFDKDVNE RNTVKGFRKF NILGTHTKVM NMEESTNGSL 
       410        420        430        440        450
AAEFRHLQLK EQKNAGTRTN EGPLIVTEEL HSLSFETQLC QPGLVIDLET 
       460        470        480        490        500
TSLPVVVISN VSQLPSGWAS ILWYNMLVAE PRNLSFFLTP PCARWAQLSE 
       510        520        530        540        550
VLSWQFSSVT KRGLNVDQLN MLGEKLLGPN ASPDGLIPWT RFCKENINDK 
       560        570        580        590        600
NFPFWLWIES ILELIKKHLL PLWNDGCIMG FISKERERAL LKDQQPGTFL 
       610        620        630        640        650
LRFSESSREG AITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN 
       660        670        680        690        700
YKVMAAENIP ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDGPKGTG 
       710        720        730        740        750
YIKTELISVS EVHPSRLQTT DNLLPMSPEE FDEVSRIVGS VEFDSMMNTV
Length:750
Mass (Da):87,335
Last modified:November 1, 1997 - v2
Checksum:i054A813522364BA6
GO
Isoform Beta (identifier: P42224-2) [UniParc]FASTAAdd to basket
Also known as: p84

The sequence of this isoform differs from the canonical sequence as follows:
     713-750: Missing.

Show »
Length:712
Mass (Da):83,043
Checksum:i31408601223700BB
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KPM9J3KPM9_HUMAN
Signal transducer and activator of ...
STAT1
714Annotation score:
D2KFR9D2KFR9_HUMAN
Signal transducer and activator of ...
STAT1
193Annotation score:
E7EPD2E7EPD2_HUMAN
Signal transducer and activator of ...
STAT1
180Annotation score:
E7ENM1E7ENM1_HUMAN
Signal transducer and activator of ...
STAT1
150Annotation score:
E9PH66E9PH66_HUMAN
Signal transducer and activator of ...
STAT1
69Annotation score:
H7BZB5H7BZB5_HUMAN
Signal transducer and activator of ...
STAT1
58Annotation score:
H7BZ88H7BZ88_HUMAN
Signal transducer and activator of ...
STAT1
40Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46A → T in ADA59516 (Ref. 2) Curated1
Sequence conflicti307S → G in BAF85293 (PubMed:14702039).Curated1
Sequence conflicti718Q → R in CAH18430 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03452130I → T. Corresponds to variant dbSNP:rs34255470Ensembl.1
Natural variantiVAR_065934165D → G in IMD31C; gain of function mutation associated with increased STAT1 phosphorylation due to impaired nuclear dephosphorylation. 2 PublicationsCorresponds to variant dbSNP:rs387906764EnsemblClinVar.1
Natural variantiVAR_065935165D → H in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906767EnsemblClinVar.1
Natural variantiVAR_065936170Y → N in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906766EnsemblClinVar.1
Natural variantiVAR_065937174C → R in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906763EnsemblClinVar.1
Natural variantiVAR_075494179N → K in IMD31C; gain of function; increases transactivation activity in response to IFNG. 1 PublicationCorresponds to variant dbSNP:rs587777628EnsemblClinVar.1
Natural variantiVAR_065815201K → N in IMD31B; not deleterious in terms of most STAT1 functions; causes abnormal splicing out of exon 8 from most mRNAs thereby decreasing protein levels by approximately 70%. 1 PublicationCorresponds to variant dbSNP:rs587776870EnsemblClinVar.1
Natural variantiVAR_065938202M → I in IMD31C. 1 Publication1
Natural variantiVAR_065939202M → V in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906762EnsemblClinVar.1
Natural variantiVAR_065940267A → V in IMD31C. 2 PublicationsCorresponds to variant dbSNP:rs387906759EnsemblClinVar.1
Natural variantiVAR_065941271Q → P in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906768EnsemblClinVar.1
Natural variantiVAR_065942274R → Q in IMD31C; gain of function; increases STAT1 phosphorylation due to impaired nuclear dephosphorylation; increases transactivation activity in response to IFNG. 2 PublicationsCorresponds to variant dbSNP:rs387906760EnsemblClinVar.1
Natural variantiVAR_065943274R → W in IMD31C; gain of function; increases phosphorylation in response to IFNG, IFNA and IL27 due to a loss of dephosphorylation. 3 PublicationsCorresponds to variant dbSNP:rs387906758EnsemblClinVar.1
Natural variantiVAR_075495278K → E in IMD31C; gain of function; increases phosphorylation in response to IFNG and IFNA due to a loss of dephosphorylation. 1 PublicationCorresponds to variant dbSNP:rs863223398EnsemblClinVar.1
Natural variantiVAR_075496285Q → R in IMD31C; gain of function; increases transactivation activity in response to IFNG. 1 PublicationCorresponds to variant dbSNP:rs587777629EnsemblClinVar.1
Natural variantiVAR_065944286K → I in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906761EnsemblClinVar.1
Natural variantiVAR_065945288T → A in IMD31C. 1 PublicationCorresponds to variant dbSNP:rs387906765EnsemblClinVar.1
Natural variantiVAR_075497298K → N in IMD31C; gain of function; increases basal STAT1 phosphorylation levels which are 10-20 fold higher than controls after IFNG stimulation. 1 Publication1
Natural variantiVAR_065816320E → Q in IMD31A; affects the DNA-binding activity of the protein. 1 PublicationCorresponds to variant dbSNP:rs137852680EnsemblClinVar.1
Natural variantiVAR_075498384G → D in IMD31C; gain of function; increases phosphorylation in response to IFNG and IFNA due to a loss of dephosphorylation. 1 PublicationCorresponds to variant dbSNP:rs796065052EnsemblClinVar.1
Natural variantiVAR_075499385T → M in IMD31C; gain of function; increases phosphorylation in response to IFNG, IFNA and IL27 due to a loss of dephosphorylation. 2 PublicationsCorresponds to variant dbSNP:rs587777630EnsemblClinVar.1
Natural variantiVAR_065817463Q → H in IMD31A; affects the DNA-binding activity of the protein. 1 PublicationCorresponds to variant dbSNP:rs137852679EnsemblClinVar.1
Natural variantiVAR_036001491P → A in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_018265600L → P in IMD31B; found in an infant who died of a viral-like illness associated with complete STAT1 deficiency. 1 PublicationCorresponds to variant dbSNP:rs137852678EnsemblClinVar.1
Natural variantiVAR_068713637K → E in IMD31A; affects both phosphorylation and DNA-binding activity; results in impaired STAT1-mediated cellular response to IFN-gamma and interleukin-27. 1 PublicationCorresponds to variant dbSNP:rs587777705EnsemblClinVar.1
Natural variantiVAR_068714673K → R in IMD31A; impairs tyrosine phosphorylation; results in impaired STAT1-mediated cellular response to IFN-gamma and interleukin-27. 1 PublicationCorresponds to variant dbSNP:rs587777704EnsemblClinVar.1
Natural variantiVAR_075500701Y → C in IMD31B; disrupts transactivation activity in response to IFNG. 1 Publication1
Natural variantiVAR_018266706L → S in IMD31A; loss of GAF and ISGF3 activation; impairs the nuclear accumulation of GAF but not of ISGF3 in heterozygous cells stimulated by IFNs; affects phosphorylation of the protein. 2 PublicationsCorresponds to variant dbSNP:rs137852677EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006282713 – 750Missing in isoform Beta. 2 PublicationsAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97935 mRNA Translation: AAB64012.1
M97936 mRNA No translation available.
GU211347 mRNA Translation: ADA59516.1
AY865620 Genomic DNA Translation: AAW56072.1
AK292604 mRNA Translation: BAF85293.1
AK315002 mRNA Translation: BAG37497.1
CR749636 mRNA Translation: CAH18430.1
BT007241 mRNA Translation: AAP35905.1
AC067945 Genomic DNA Translation: AAY24183.1
CH471058 Genomic DNA Translation: EAX10850.1
CH471058 Genomic DNA Translation: EAX10851.1
CH471058 Genomic DNA Translation: EAX10852.1
CH471058 Genomic DNA Translation: EAX10855.1
BC002704 mRNA Translation: AAH02704.1
U18662 Genomic DNA No translation available.
U18663 Genomic DNA No translation available.
U18664 Genomic DNA No translation available.
U18665 Genomic DNA No translation available.
U18666 Genomic DNA No translation available.
U18667 Genomic DNA No translation available.
U18668 Genomic DNA No translation available.
U18669 Genomic DNA No translation available.
U18670 Genomic DNA No translation available.
CCDSiCCDS2309.1 [P42224-1]
CCDS42793.1 [P42224-2]
PIRiA46159
RefSeqiNP_009330.1, NM_007315.3 [P42224-1]
NP_644671.1, NM_139266.2 [P42224-2]
XP_006712781.1, XM_006712718.1 [P42224-1]
UniGeneiHs.642990
Hs.743244

Genome annotation databases

EnsembliENST00000361099; ENSP00000354394; ENSG00000115415 [P42224-1]
ENST00000392322; ENSP00000376136; ENSG00000115415 [P42224-2]
ENST00000409465; ENSP00000386244; ENSG00000115415 [P42224-1]
GeneIDi6772
KEGGihsa:6772
UCSCiuc002usj.3 human [P42224-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs
STAT1base

STAT1 mutation db

Wikipedia

STAT1 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97935 mRNA Translation: AAB64012.1
M97936 mRNA No translation available.
GU211347 mRNA Translation: ADA59516.1
AY865620 Genomic DNA Translation: AAW56072.1
AK292604 mRNA Translation: BAF85293.1
AK315002 mRNA Translation: BAG37497.1
CR749636 mRNA Translation: CAH18430.1
BT007241 mRNA Translation: AAP35905.1
AC067945 Genomic DNA Translation: AAY24183.1
CH471058 Genomic DNA Translation: EAX10850.1
CH471058 Genomic DNA Translation: EAX10851.1
CH471058 Genomic DNA Translation: EAX10852.1
CH471058 Genomic DNA Translation: EAX10855.1
BC002704 mRNA Translation: AAH02704.1
U18662 Genomic DNA No translation available.
U18663 Genomic DNA No translation available.
U18664 Genomic DNA No translation available.
U18665 Genomic DNA No translation available.
U18666 Genomic DNA No translation available.
U18667 Genomic DNA No translation available.
U18668 Genomic DNA No translation available.
U18669 Genomic DNA No translation available.
U18670 Genomic DNA No translation available.
CCDSiCCDS2309.1 [P42224-1]
CCDS42793.1 [P42224-2]
PIRiA46159
RefSeqiNP_009330.1, NM_007315.3 [P42224-1]
NP_644671.1, NM_139266.2 [P42224-2]
XP_006712781.1, XM_006712718.1 [P42224-1]
UniGeneiHs.642990
Hs.743244

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BF5X-ray2.90A136-710[»]
1YVLX-ray3.00A/B1-683[»]
2KA6NMR-B710-750[»]
3WWTX-ray2.00A1-126[»]
DisProtiDP00962
ProteinModelPortaliP42224
SMRiP42224
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112649, 163 interactors
CORUMiP42224
DIPiDIP-46140N
IntActiP42224, 99 interactors
MINTiP42224
STRINGi9606.ENSP00000354394

Chemistry databases

BindingDBiP42224
ChEMBLiCHEMBL6101

PTM databases

CarbonylDBiP42224
iPTMnetiP42224
PhosphoSitePlusiP42224
SwissPalmiP42224

Polymorphism and mutation databases

BioMutaiSTAT1
DMDMi2507413

Proteomic databases

EPDiP42224
MaxQBiP42224
PaxDbiP42224
PeptideAtlasiP42224
PRIDEiP42224
ProteomicsDBi55491
55492 [P42224-2]

Protocols and materials databases

DNASUi6772
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361099; ENSP00000354394; ENSG00000115415 [P42224-1]
ENST00000392322; ENSP00000376136; ENSG00000115415 [P42224-2]
ENST00000409465; ENSP00000386244; ENSG00000115415 [P42224-1]
GeneIDi6772
KEGGihsa:6772
UCSCiuc002usj.3 human [P42224-1]

Organism-specific databases

CTDi6772
DisGeNETi6772
EuPathDBiHostDB:ENSG00000115415.18
GeneCardsiSTAT1
HGNCiHGNC:11362 STAT1
HPAiCAB004049
HPA000931
HPA000982
MalaCardsiSTAT1
MIMi600555 gene
613796 phenotype
614162 phenotype
614892 phenotype
neXtProtiNX_P42224
OpenTargetsiENSG00000115415
Orphaneti391487 Autoimmune enteropathy and endocrinopathy-susceptibility to chronic infections syndrome
319595 Mendelian susceptibility to mycobacterial diseases due to partial STAT1 deficiency
391311 Susceptibility to viral and mycobacterial infections
PharmGKBiPA36183
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3667 Eukaryota
ENOG410XPN8 LUCA
GeneTreeiENSGT00760000119236
HOVERGENiHBG071022
InParanoidiP42224
KOiK11220
OMAiAKWSQLS
OrthoDBiEOG091G03O3
PhylomeDBiP42224
TreeFamiTF318648

Enzyme and pathway databases

ReactomeiR-HSA-1059683 Interleukin-6 signaling
R-HSA-1169408 ISG15 antiviral mechanism
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-1839117 Signaling by cytosolic FGFR1 fusion mutants
R-HSA-186763 Downstream signal transduction
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-877300 Interferon gamma signaling
R-HSA-877312 Regulation of IFNG signaling
R-HSA-8854691 Interleukin-20 family signaling
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8984722 Interleukin-35 Signalling
R-HSA-8985947 Interleukin-9 signaling
R-HSA-9013508 NOTCH3 Intracellular Domain Regulates Transcription
R-HSA-9020956 Interleukin-27 signaling
R-HSA-9020958 Interleukin-21 signaling
R-HSA-909733 Interferon alpha/beta signaling
R-HSA-912694 Regulation of IFNA signaling
R-HSA-982772 Growth hormone receptor signaling
SignaLinkiP42224
SIGNORiP42224

Miscellaneous databases

ChiTaRSiSTAT1 human
EvolutionaryTraceiP42224
GeneWikiiSTAT1
GenomeRNAii6772
PMAP-CutDBiP42224
PROiPR:P42224
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115415 Expressed in 234 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_STAT1
ExpressionAtlasiP42224 baseline and differential
GenevisibleiP42224 HS

Family and domain databases

CDDicd10372 SH2_STAT1, 1 hit
Gene3Di1.10.532.10, 1 hit
1.10.8.1200, 1 hit
2.60.40.630, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR008967 p53-like_TF_DNA-bd
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR001217 STAT
IPR038295 STAT1_C_sf
IPR035859 STAT1_SH2
IPR022752 STAT1_TAZ2-bd_C
IPR036535 STAT_N_sf
IPR013800 STAT_TF_alpha
IPR015988 STAT_TF_coiled-coil
IPR013801 STAT_TF_DNA-bd
IPR012345 STAT_TF_DNA-bd_N
IPR013799 STAT_TF_prot_interaction
PANTHERiPTHR11801 PTHR11801, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF12162 STAT1_TAZ2bind, 1 hit
PF01017 STAT_alpha, 1 hit
PF02864 STAT_bind, 1 hit
PF02865 STAT_int, 1 hit
SMARTiView protein in SMART
SM00964 STAT_int, 1 hit
SUPFAMiSSF47655 SSF47655, 1 hit
SSF48092 SSF48092, 1 hit
SSF49417 SSF49417, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTAT1_HUMAN
AccessioniPrimary (citable) accession number: P42224
Secondary accession number(s): A8K989
, B2RCA0, D2KFR8, D3DPI7, Q53S88, Q53XW4, Q68D00, Q9UDL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 224 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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