UniProtKB - P42222 (ERR3_YEAST)
Protein
Enolase-related protein 3
Gene
ERR3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalytic activityi
- EC:4.2.1.11
Cofactori
Mg2+By similarity
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2), Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3), Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1)
- Phosphoglycerate kinase (PGK1)
- Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 1 (GPM1), Phosphoglycerate mutase 3 (GPM3)
- Enolase 2 (ENO2), Enolase-related protein 2 (ERR2), Enolase-related protein 1 (ERR1), Enolase-related protein 3 (ERR3), Enolase 1 (ENO1)
- Pyruvate kinase 1 (CDC19), Pyruvate kinase 2 (PYK2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 160 | SubstrateBy similarity | 1 | |
Binding sitei | 169 | SubstrateBy similarity | 1 | |
Active sitei | 212 | Proton donorBy similarity | 1 | |
Metal bindingi | 247 | MagnesiumBy similarity | 1 | |
Metal bindingi | 296 | MagnesiumBy similarity | 1 | |
Binding sitei | 296 | SubstrateBy similarity | 1 | |
Metal bindingi | 321 | MagnesiumBy similarity | 1 | |
Binding sitei | 321 | SubstrateBy similarity | 1 | |
Active sitei | 346 | Proton acceptorBy similarity | 1 | |
Binding sitei | 397 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: InterPro
- phosphopyruvate hydratase activity Source: SGD
GO - Biological processi
- glycolytic process Source: GO_Central
- regulation of vacuole fusion, non-autophagic Source: GO_Central
Keywordsi
Molecular function | Lyase |
Biological process | Glycolysis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-70171, Glycolysis R-SCE-70263, Gluconeogenesis |
SABIO-RKi | P42222 |
UniPathwayi | UPA00109;UER00187 |
Names & Taxonomyi
Protein namesi | Recommended name: Enolase-related protein 3 (EC:4.2.1.11)Alternative name(s): 2-phospho-D-glycerate hydro-lyase 2-phosphoglycerate dehydratase |
Gene namesi | Name:ERR3 Ordered Locus Names:YMR323W ORF Names:YM9924.15 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004942, ERR3 |
VEuPathDBi | FungiDB:YMR323W |
Subcellular locationi
Cytosol
- phosphopyruvate hydratase complex Source: GO_Central
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000134065 | 1 – 437 | Enolase-related protein 3Add BLAST | 437 |
Proteomic databases
PaxDbi | P42222 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35502, 5 interactors |
DIPi | DIP-4046N |
IntActi | P42222, 1 interactor |
MINTi | P42222 |
STRINGi | 4932.YMR323W |
Miscellaneous databases
RNActi | P42222, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 373 – 376 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the enolase family.Curated
Phylogenomic databases
eggNOGi | KOG2670, Eukaryota |
GeneTreei | ENSGT00950000182805 |
HOGENOMi | CLU_031223_0_0_1 |
InParanoidi | P42222 |
OMAi | SRICKAV |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P42222-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSITKVHART VYDSRGNPTV EVEITTENGL FRAIVPSGAS TGIHEAVELR
60 70 80 90 100
DGNKSEWMGK GVTKAVSNVN SIIGPALIKS DLCVTNQKGI DELMISLDGT
110 120 130 140 150
SNKSRLGANA ILGVSLCVAR AAAAQKGITL YKYIAELADA RQDPFVIPVP
160 170 180 190 200
FFNVLNGGAH AGGSLAMQEF KIAPVGAQSF AEAMRMGSEV YHHLKILAKE
210 220 230 240 250
QYGPSAGNVG DEGGVAPDID TAEDALDMIV KAINICGYEG RVKVGIDSAP
260 270 280 290 300
SVFYKDGKYD LNFKEPNSDP SHWLSPAQLA EYYHSLLKKY PIISLEDPYA
310 320 330 340 350
EDDWSSWSAF LKTVNVQIIA DDLTCTNKTR IARAIEEKCA NTLLLKLNQI
360 370 380 390 400
GTLTESIEAA NQAFDAGWGV MISHRSGETE DPFIADLVVG LRCGQIKSGA
410 420 430
LSRSERLAKY NELLRIEEEL GDDCIYAGHR FHDGNKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54141 Genomic DNA Translation: CAA90841.1 BK006946 Genomic DNA Translation: DAA10224.1 |
PIRi | S69881 |
RefSeqi | NP_014056.3, NM_001182836.3 |
Genome annotation databases
EnsemblFungii | YMR323W_mRNA; YMR323W; YMR323W |
GeneIDi | 855373 |
KEGGi | sce:YMR323W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54141 Genomic DNA Translation: CAA90841.1 BK006946 Genomic DNA Translation: DAA10224.1 |
PIRi | S69881 |
RefSeqi | NP_014056.3, NM_001182836.3 |
3D structure databases
SMRi | P42222 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35502, 5 interactors |
DIPi | DIP-4046N |
IntActi | P42222, 1 interactor |
MINTi | P42222 |
STRINGi | 4932.YMR323W |
Proteomic databases
PaxDbi | P42222 |
Genome annotation databases
EnsemblFungii | YMR323W_mRNA; YMR323W; YMR323W |
GeneIDi | 855373 |
KEGGi | sce:YMR323W |
Organism-specific databases
SGDi | S000004942, ERR3 |
VEuPathDBi | FungiDB:YMR323W |
Phylogenomic databases
eggNOGi | KOG2670, Eukaryota |
GeneTreei | ENSGT00950000182805 |
HOGENOMi | CLU_031223_0_0_1 |
InParanoidi | P42222 |
OMAi | SRICKAV |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
Reactomei | R-SCE-70171, Glycolysis R-SCE-70263, Gluconeogenesis |
SABIO-RKi | P42222 |
Miscellaneous databases
PROi | PR:P42222 |
RNActi | P42222, protein |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ERR3_YEAST | |
Accessioni | P42222Primary (citable) accession number: P42222 Secondary accession number(s): D6W0F0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families