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UniProtKB - P42210 (ASPR_HORVU)
Protein
Phytepsin
Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Functioni
Involved in the breakdown of propeptides of storage proteins in protein-storage vacuoles.
By similarityCatalytic activityi
- Prefers hydrophobic residues Phe, Val, Ile, Leu, and Ala at P1 and P1', but also cleaves -Phe-|-Asp- and -Asp-|-Asp- bonds in 2S albumin from plant seeds. EC:3.4.23.40
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 102 | 1 | ||
Active sitei | 289 | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
GO - Biological processi
- lipid metabolic process Source: InterPro
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Protein family/group databases
MEROPSi | A01.020 |
Names & Taxonomyi
Protein namesi | Recommended name: Phytepsin (EC:3.4.23.40)Alternative name(s): Aspartic proteinase Cleaved into the following 4 chains: |
Organismi | Hordeum vulgare (Barley) |
Taxonomic identifieri | 4513 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Pooideae › Triticodae › Triticeae › Hordeinae › Hordeum |
Subcellular locationi
Vacuole
- Vacuole 1 Publication
Vacuole
- vacuole Source: UniProtKB-SubCell
Keywords - Cellular componenti
VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
PropeptideiPRO_0000025903 | 28 – 66 | Activation peptideAdd BLAST | 39 | |
ChainiPRO_0000025904 | 67 – 377 | Phytepsin 32 kDa subunitAdd BLAST | 311 | |
ChainiPRO_0000025905 | 67 – ? | Phytepsin 29 kDa subunit | ||
ChainiPRO_0000025906 | 378 – 508 | Phytepsin 16 kDa subunitAdd BLAST | 131 | |
ChainiPRO_0000025907 | 422 – 508 | Phytepsin 11 kDa subunitAdd BLAST | 87 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 115 ↔ 121 | Combined sources1 Publication | ||
Disulfide bondi | 280 ↔ 284 | Combined sources1 Publication | ||
Disulfide bondi | 319 ↔ 413 | Combined sources1 Publication | ||
Disulfide bondi | 344 ↔ 385 | Combined sources1 Publication | ||
Disulfide bondi | 350 ↔ 382 | Combined sources1 Publication | ||
Glycosylationi | 399 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 427 ↔ 464 | Combined sources1 Publication |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 377 – 378 | Cleavage | 2 | |
Sitei | 421 – 422 | Cleavage | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PRIDEi | P42210 |
Expressioni
Tissue specificityi
Embryo and leaf.
Gene expression databases
ExpressionAtlasi | P42210, baseline and differential |
Interactioni
Subunit structurei
Heterodimer of two subunits (29 kDa and 11 kDa) processed from the precursor molecule. A large enzyme (32 kDa and 16 kDa) is an intermediate precursor form.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P42210 |
SMRi | P42210 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42210 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 84 – 505 | Peptidase A1PROSITE-ProRule annotationAdd BLAST | 422 | |
Domaini | 314 – 419 | Saposin B-typePROSITE-ProRule annotationAdd BLAST | 106 |
Sequence similaritiesi
Belongs to the peptidase A1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
OMAi | GVECANL |
Family and domain databases
CDDi | cd06098, phytepsin, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf IPR033869, Phytepsin IPR007856, SapB_1 IPR008138, SapB_2 IPR011001, Saposin-like IPR008139, SaposinB_dom |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit PF05184, SapB_1, 1 hit PF03489, SapB_2, 1 hit |
PRINTSi | PR00792, PEPSIN |
SMARTi | View protein in SMART SM00741, SapB, 1 hit |
SUPFAMi | SSF47862, SSF47862, 1 hit SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit PS50015, SAP_B, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P42210-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTRGLALAL LAAVLLLQTV LPAASEAEGL VRIALKKRPI DRNSRVATGL
60 70 80 90 100
SGGEEQPLLS GANPLRSEEE GDIVALKNYM NAQYFGEIGV GTPPQKFTVI
110 120 130 140 150
FDTGSSNLWV PSAKCYFSIA CYLHSRYKAG ASSTYKKNGK PAAIQYGTGS
160 170 180 190 200
IAGYFSEDSV TVGDLVVKDQ EFIEATKEPG ITFLVAKFDG ILGLGFKEIS
210 220 230 240 250
VGKAVPVWYK MIEQGLVSDP VFSFWLNRHV DEGEGGEIIF GGMDPKHYVG
260 270 280 290 300
EHTYVPVTQK GYWQFDMGDV LVGGKSTGFC AGGCAAIADS GTSLLAGPTA
310 320 330 340 350
IITEINEKIG AAGVVSQECK TIVSQYGQQI LDLLLAETQP KKICSQVGLC
360 370 380 390 400
TFDGTRGVSA GIRSVVDDEP VKSNGLRADP MCSACEMAVV WMQNQLAQNK
410 420 430 440 450
TQDLILDYVN QLCNRLPSPM GESAVDCGSL GSMPDIEFTI GGKKFALKPE
460 470 480 490 500
EYILKVGEGA AAQCISGFTA MDIPPPRGPL WILGDVFMGP YHTVFDYGKL
RIGFAKAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56136 mRNA Translation: CAA39602.1 |
PIRi | S19697 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56136 mRNA Translation: CAA39602.1 |
PIRi | S19697 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QDM | X-ray | 2.30 | A/B/C | 31-508 | [»] | |
AlphaFoldDBi | P42210 | |||||
SMRi | P42210 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
MEROPSi | A01.020 |
Proteomic databases
PRIDEi | P42210 |
Genome annotation databases
Phylogenomic databases
OMAi | GVECANL |
Miscellaneous databases
EvolutionaryTracei | P42210 |
Gene expression databases
ExpressionAtlasi | P42210, baseline and differential |
Family and domain databases
CDDi | cd06098, phytepsin, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf IPR033869, Phytepsin IPR007856, SapB_1 IPR008138, SapB_2 IPR011001, Saposin-like IPR008139, SaposinB_dom |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit PF05184, SapB_1, 1 hit PF03489, SapB_2, 1 hit |
PRINTSi | PR00792, PEPSIN |
SMARTi | View protein in SMART SM00741, SapB, 1 hit |
SUPFAMi | SSF47862, SSF47862, 1 hit SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit PS50015, SAP_B, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ASPR_HORVU | |
Accessioni | P42210Primary (citable) accession number: P42210 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families