UniProtKB - P42161 (IFNG_CANLF)
Interferon gamma
IFNG
Functioni
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity).
Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: GO_Central
- interferon-gamma receptor binding Source: InterPro
GO - Biological processi
- adaptive immune response Source: GO_Central
- astrocyte activation Source: Ensembl
- defense response to virus Source: UniProtKB-KW
- extrinsic apoptotic signaling pathway Source: Ensembl
- humoral immune response Source: GO_Central
- interferon-gamma-mediated signaling pathway Source: Ensembl
- macrophage activation involved in immune response Source: Ensembl
- macrophage differentiation Source: Ensembl
- microglial cell activation Source: Ensembl
- negative regulation of interleukin-17 production Source: Ensembl
- negative regulation of smooth muscle cell proliferation Source: Ensembl
- negative regulation of tau-protein kinase activity Source: Ensembl
- negative regulation of transcription, DNA-templated Source: Ensembl
- positive regulation of amyloid-beta formation Source: Ensembl
- positive regulation of autophagy Source: UniProtKB
- positive regulation of calcidiol 1-monooxygenase activity Source: Ensembl
- positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response Source: Ensembl
- positive regulation of chemokine production Source: Ensembl
- positive regulation of core promoter binding Source: Ensembl
- positive regulation of epithelial cell migration Source: Ensembl
- positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity Source: Ensembl
- positive regulation of fructose 1,6-bisphosphate metabolic process Source: Ensembl
- positive regulation of inflammatory response Source: Ensembl
- positive regulation of interleukin-12 production Source: Ensembl
- positive regulation of interleukin-23 production Source: Ensembl
- positive regulation of interleukin-6 production Source: Ensembl
- positive regulation of killing of cells of other organism Source: Ensembl
- positive regulation of membrane protein ectodomain proteolysis Source: Ensembl
- positive regulation of neurogenesis Source: Ensembl
- positive regulation of neuron death Source: Ensembl
- positive regulation of nitric oxide biosynthetic process Source: Ensembl
- positive regulation of osteoclast differentiation Source: Ensembl
- positive regulation of peptidyl-serine phosphorylation of STAT protein Source: Ensembl
- positive regulation of protein-containing complex assembly Source: Ensembl
- positive regulation of protein deacetylation Source: Ensembl
- positive regulation of protein import into nucleus Source: Ensembl
- positive regulation of protein serine/threonine kinase activity Source: Ensembl
- positive regulation of smooth muscle cell apoptotic process Source: Ensembl
- positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Source: Ensembl
- positive regulation of tyrosine phosphorylation of STAT protein Source: Ensembl
- positive regulation of vitamin D biosynthetic process Source: Ensembl
- regulation of growth Source: UniProtKB-KW
- regulation of insulin secretion Source: Ensembl
- regulation of protein ADP-ribosylation Source: Ensembl
Keywordsi
Molecular function | Cytokine |
Biological process | Antiviral defense, Growth regulation |
Enzyme and pathway databases
Reactomei | R-CFA-877300, Interferon gamma signaling R-CFA-877312, Regulation of IFNG signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon gammaShort name: IFN-gamma |
Gene namesi | Name:IFNG |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:41879, IFNG |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | By similarityAdd BLAST | 23 | |
ChainiPRO_0000016437 | 24 – 166 | Interferon gammaAdd BLAST | 143 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | Pyrrolidone carboxylic acidBy similarity | 1 | |
Glycosylationi | 39 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 106 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, Pyrrolidone carboxylic acidProteomic databases
PaxDbi | P42161 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSCAFG00000000408, Expressed in stomach and 25 other tissues |
Interactioni
Subunit structurei
Homodimer.
Interacts with IFNGR1 (via extracellular domain); this interaction promotes IFNGR1 dimerization.
By similarityGO - Molecular functioni
- cytokine activity Source: GO_Central
- interferon-gamma receptor binding Source: InterPro
Protein-protein interaction databases
STRINGi | 9615.ENSCAFP00000000588 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SBGW, Eukaryota |
GeneTreei | ENSGT00390000007831 |
HOGENOMi | CLU_135106_0_0_1 |
InParanoidi | P42161 |
OMAi | MNDLSPK |
OrthoDBi | 1382686at2759 |
TreeFami | TF336308 |
Family and domain databases
Gene3Di | 1.20.1250.10, 1 hit |
InterProi | View protein in InterPro IPR009079, 4_helix_cytokine-like_core IPR002069, Interferon_gamma |
PANTHERi | PTHR11419, PTHR11419, 1 hit |
Pfami | View protein in Pfam PF00714, IFN-gamma, 1 hit |
PIRSFi | PIRSF001936, IFN-gamma, 1 hit |
SUPFAMi | SSF47266, SSF47266, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MNYTSYILAF QLCVILCSSG CNCQAMFFKE IENLKEYFNA SNPDVSDGGS
60 70 80 90 100
LFVDILKKWR EESDKTIIQS QIVSFYLKLF DNFKDNQIIQ RSMDTIKEDM
110 120 130 140 150
LGKFLNSSTS KREDFLKLIQ IPVNDLQVQR KAINELIKVM NDLSPRSNLR
160
KRKRSQNLFR GRRASK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | S → L in AAB22724 (PubMed:1640120).Curated | 1 | |
Sequence conflicti | 22 | N → Y in AAB22724 (PubMed:1640120).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF126247 mRNA Translation: AAD31423.1 EF095772 mRNA Translation: ABL01503.1 S41201 mRNA Translation: AAB22724.2 |
RefSeqi | NP_001003174.1, NM_001003174.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF126247 mRNA Translation: AAD31423.1 EF095772 mRNA Translation: ABL01503.1 S41201 mRNA Translation: AAB22724.2 |
RefSeqi | NP_001003174.1, NM_001003174.1 |
3D structure databases
SMRi | P42161 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9615.ENSCAFP00000000588 |
Proteomic databases
PaxDbi | P42161 |
Genome annotation databases
Organism-specific databases
CTDi | 3458 |
VGNCi | VGNC:41879, IFNG |
Phylogenomic databases
eggNOGi | ENOG502SBGW, Eukaryota |
GeneTreei | ENSGT00390000007831 |
HOGENOMi | CLU_135106_0_0_1 |
InParanoidi | P42161 |
OMAi | MNDLSPK |
OrthoDBi | 1382686at2759 |
TreeFami | TF336308 |
Enzyme and pathway databases
Reactomei | R-CFA-877300, Interferon gamma signaling R-CFA-877312, Regulation of IFNG signaling |
Gene expression databases
Bgeei | ENSCAFG00000000408, Expressed in stomach and 25 other tissues |
Family and domain databases
Gene3Di | 1.20.1250.10, 1 hit |
InterProi | View protein in InterPro IPR009079, 4_helix_cytokine-like_core IPR002069, Interferon_gamma |
PANTHERi | PTHR11419, PTHR11419, 1 hit |
Pfami | View protein in Pfam PF00714, IFN-gamma, 1 hit |
PIRSFi | PIRSF001936, IFN-gamma, 1 hit |
SUPFAMi | SSF47266, SSF47266, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IFNG_CANLF | |
Accessioni | P42161Primary (citable) accession number: P42161 Secondary accession number(s): A1E294, Q9TVA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families