UniProtKB - P42128 (FOXK1_MOUSE)
Forkhead box protein K1
Foxk1
Functioni
Caution
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 291 – 386 | Fork-headPROSITE-ProRule annotationAdd BLAST | 96 |
GO - Molecular functioni
- 14-3-3 protein binding Source: MGI
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- canonical glycolysis Source: MGI
- cell differentiation Source: UniProtKB-KW
- cellular glucose homeostasis Source: MGI
- muscle organ development Source: UniProtKB-KW
- negative regulation of autophagy Source: MGI
- negative regulation of cell cycle Source: UniProtKB
- negative regulation of cell growth Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of glucose metabolic process Source: MGI
- regulation of transcription by RNA polymerase II Source: NTNU_SB
- response to starvation Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Myogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-5689603, UCH proteinases |
Names & Taxonomyi
Protein namesi | Recommended name: Forkhead box protein K11 PublicationAlternative name(s): Myocyte nuclear factor2 Publications Short name: MNF2 Publications |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1347488, Foxk1 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Note: Translocation to the nucleus is regulated by phosphorylation: phosphorylation by GSK3 (GSK3A or GSK3B) promotes interaction with 14-3-3 proteins and sequestration in the cytoplasm (PubMed:29861159). Dephosphorylation promotes translocation to the nucleus (PubMed:29861159).1 Publication
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 333 | K → A: Decreases DNA-binding. No effect on transcriptional repression. 1 Publication | 1 | |
Mutagenesisi | 340 | R → A: Abolishes DNA-binding. No effect on transcriptional repression. 1 Publication | 1 | |
Mutagenesisi | 402 – 406 | SAPAS → AAPAA: Decreased phosphorylation by GSK3A and interaction with 14-3-3 proteins. 1 Publication | 5 | |
Mutagenesisi | 427 – 431 | SREGS → AREGA: Decreased phosphorylation by GSK3A and interaction with 14-3-3 proteins. 1 Publication | 5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000091857 | 2 – 719 | Forkhead box protein K1Add BLAST | 718 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 87 | PhosphoserineBy similarity | 1 | |
Modified residuei | 147 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 177 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 209 | PhosphoserineCombined sources | 1 | |
Modified residuei | 225 | PhosphoserineCombined sources | 1 | |
Modified residuei | 229 | PhosphoserineCombined sources | 1 | |
Modified residuei | 231 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 233 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 239 | PhosphoserineCombined sources | 1 | |
Modified residuei | 243 | PhosphoserineCombined sources | 1 | |
Modified residuei | 281 | PhosphoserineCombined sources | 1 | |
Modified residuei | 285 | PhosphoserineCombined sources | 1 | |
Modified residuei | 402 | Phosphoserine1 Publication | 1 | |
Modified residuei | 406 | Phosphoserine1 Publication | 1 | |
Modified residuei | 408 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 414 | PhosphoserineBy similarity | 1 | |
Modified residuei | 422 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 427 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 431 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 445 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | P42128 |
jPOSTi | P42128 |
MaxQBi | P42128 |
PaxDbi | P42128 |
PeptideAtlasi | P42128 |
PRIDEi | P42128 |
ProteomicsDBi | 271796 |
PTM databases
iPTMneti | P42128 |
PhosphoSitePlusi | P42128 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000056493, Expressed in blood and 280 other tissues |
Genevisiblei | P42128, MM |
Interactioni
Subunit structurei
Interacts with SIN3A and SIN3B (via PAH2) to form a complex which represses transcription (PubMed:10620510).
Component of SIN3A-, but not SIN3B-, containing multiprotein complexes (PubMed:25402684).
Interacts with FOXO4 and MEF2C; both interactions inhibit FOXO4 and MEF2C transactivation activity (PubMed:22956541).
Interacts (when phosphorylated) with YWHAE/14-3-3-epsilon; promotes sequestration in the cytoplasm and leads to impaired ability to bind DNA (PubMed:29861159).
Interacts with FHL2 (PubMed:20013826).
Interacts with SRF (By similarity).
Interacts with DVL2 and DVL3; the interaction induces DVL2 nuclear translocation (By similarity).
Interacts with BAP1 (when phosphorylated) (By similarity).
By similarity5 PublicationsBinary interactionsi
P42128
With | #Exp. | IntAct |
---|---|---|
Sin3b - isoform 2 [Q62141-2] | 11 | EBI-878270,EBI-591466 |
GO - Molecular functioni
- 14-3-3 protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201459, 5 interactors |
IntActi | P42128, 2 interactors |
MINTi | P42128 |
STRINGi | 10090.ENSMUSP00000072616 |
Miscellaneous databases
RNActi | P42128, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P42128 |
SMRi | P42128 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42128 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 109 – 161 | FHAPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 40 | Interaction with SIN3A and SIN3B2 PublicationsAdd BLAST | 39 | |
Regioni | 81 – 406 | Required for interaction with FOXO4 and MEF2C1 PublicationAdd BLAST | 326 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 82 | Ala-richAdd BLAST | 73 | |
Compositional biasi | 35 – 63 | Pro-richAdd BLAST | 29 |
Phylogenomic databases
eggNOGi | KOG2294, Eukaryota |
GeneTreei | ENSGT00940000159507 |
HOGENOMi | CLU_022344_0_0_1 |
InParanoidi | P42128 |
OMAi | VTIGQHH |
OrthoDBi | 1270467at2759 |
PhylomeDBi | P42128 |
TreeFami | TF325718 |
Family and domain databases
CDDi | cd00059, FH, 1 hit cd00060, FHA, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000253, FHA_dom IPR001766, Fork_head_dom IPR008984, SMAD_FHA_dom_sf IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00498, FHA, 1 hit PF00250, Forkhead, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit SM00240, FHA, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS50006, FHA_DOMAIN, 1 hit PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAEVGEDSGA RALLALRSAP CSPVLCAAAA AAAFPATTSP PPPAQPPPGP
60 70 80 90 100
PALPAEPGPG PVPSTVATAT TTAPALVAAA AASVRQSPGP ALARLEGREF
110 120 130 140 150
EFLMRQPSVT IGRNSSQGSV DLSMGLSSFI SRRHLQLSFQ EPHFYLRCLG
160 170 180 190 200
KNGVFVDGAF QRRGAPALQL PQQCTFRFPS TAIKIQFTSL YHKEEAPASP
210 220 230 240 250
LRPLYPQISP LKIHIPEPDL RSLVSPIPSP TGTISVPNSC PASPRGAGSS
260 270 280 290 300
SYRFVQNVTS DLQLAAEFAA KAASEQQADA SGGDSPKDES KPPYSYAQLI
310 320 330 340 350
VQAISSAQDR QLTLSGIYAH ITKHYPYYRT ADKGWQNSIR HNLSLNRYFI
360 370 380 390 400
KVPRSQEEPG KGSFWRIDPA SEAKLVEQAF RKRRQRGVSC FRTPFGPLSS
410 420 430 440 450
RSAPASPTHP GLMSPRSSGL QTPECLSREG SPIPHDPDLG SKLASVPEYR
460 470 480 490 500
YSQSAPGSPV SAQPVIMAVP PRPSNLVAKP VAYMPASIVT SQQPSGHAIH
510 520 530 540 550
VVQQAPTVTM VRVVTTSANS ANGYILASQG STGTSHDTAG TAVLDLGNEA
560 570 580 590 600
RGLEEKPTIA FATIPAASRV IQTVASQMAP GVPGHTVTIL QPATPVTIGQ
610 620 630 640 650
HHLPVRAVTQ NGKHAVPTNS LTGNAYALSS PLQLLAAQAS SSTPVVITRV
660 670 680 690 700
CEVGPEEPAA AVSVAANAAP TPAASTTTSA SSSGEPEVKR SRVEEPGGTA
710
TTQPTAMAAT GPQGPGTGE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JG71 | A0A0G2JG71_MOUSE | Forkhead box protein K1 | Foxk1 | 181 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 | Missing in AAA37529 (PubMed:8007964).Curated | 1 | |
Sequence conflicti | 43 | Missing in AAB69641 (PubMed:9271401).Curated | 1 | |
Sequence conflicti | 402 – 403 | SA → RS in AAA37529 (PubMed:8007964).Curated | 2 | |
Sequence conflicti | 407 – 411 | PTHPG → HTSHA in AAB69641 (PubMed:9271401).Curated | 5 | |
Sequence conflicti | 551 | R → A in AAA37529 (PubMed:8007964).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26507 mRNA Translation: AAA37529.1 Frameshift. AK144418 mRNA Translation: BAE25880.1 Different initiation. AK147375 mRNA Translation: BAE27871.1 U95016 mRNA Translation: AAB69641.1 Frameshift. |
CCDSi | CCDS19826.1 |
PIRi | A56051 |
RefSeqi | NP_951031.2, NM_199068.2 |
Genome annotation databases
Ensembli | ENSMUST00000072837; ENSMUSP00000072616; ENSMUSG00000056493 |
GeneIDi | 17425 |
KEGGi | mmu:17425 |
UCSCi | uc009aio.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26507 mRNA Translation: AAA37529.1 Frameshift. AK144418 mRNA Translation: BAE25880.1 Different initiation. AK147375 mRNA Translation: BAE27871.1 U95016 mRNA Translation: AAB69641.1 Frameshift. |
CCDSi | CCDS19826.1 |
PIRi | A56051 |
RefSeqi | NP_951031.2, NM_199068.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2A3S | NMR | - | A | 289-389 | [»] | |
2D2W | NMR | - | A | 289-389 | [»] | |
BMRBi | P42128 | |||||
SMRi | P42128 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201459, 5 interactors |
IntActi | P42128, 2 interactors |
MINTi | P42128 |
STRINGi | 10090.ENSMUSP00000072616 |
PTM databases
iPTMneti | P42128 |
PhosphoSitePlusi | P42128 |
Proteomic databases
EPDi | P42128 |
jPOSTi | P42128 |
MaxQBi | P42128 |
PaxDbi | P42128 |
PeptideAtlasi | P42128 |
PRIDEi | P42128 |
ProteomicsDBi | 271796 |
Protocols and materials databases
Antibodypediai | 11250, 213 antibodies |
DNASUi | 17425 |
Genome annotation databases
Ensembli | ENSMUST00000072837; ENSMUSP00000072616; ENSMUSG00000056493 |
GeneIDi | 17425 |
KEGGi | mmu:17425 |
UCSCi | uc009aio.1, mouse |
Organism-specific databases
CTDi | 221937 |
MGIi | MGI:1347488, Foxk1 |
Phylogenomic databases
eggNOGi | KOG2294, Eukaryota |
GeneTreei | ENSGT00940000159507 |
HOGENOMi | CLU_022344_0_0_1 |
InParanoidi | P42128 |
OMAi | VTIGQHH |
OrthoDBi | 1270467at2759 |
PhylomeDBi | P42128 |
TreeFami | TF325718 |
Enzyme and pathway databases
Reactomei | R-MMU-5689603, UCH proteinases |
Miscellaneous databases
BioGRID-ORCSi | 17425, 2 hits in 52 CRISPR screens |
ChiTaRSi | Foxk1, mouse |
EvolutionaryTracei | P42128 |
PROi | PR:P42128 |
RNActi | P42128, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000056493, Expressed in blood and 280 other tissues |
Genevisiblei | P42128, MM |
Family and domain databases
CDDi | cd00059, FH, 1 hit cd00060, FHA, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000253, FHA_dom IPR001766, Fork_head_dom IPR008984, SMAD_FHA_dom_sf IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00498, FHA, 1 hit PF00250, Forkhead, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit SM00240, FHA, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS50006, FHA_DOMAIN, 1 hit PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FOXK1_MOUSE | |
Accessioni | P42128Primary (citable) accession number: P42128 Secondary accession number(s): O35939, Q3UHI6, Q3UN67 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 28, 2006 | |
Last modified: | April 7, 2021 | |
This is version 199 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references