UniProtKB - P42106 (QDOI_BACSU)
Protein
Quercetin 2,3-dioxygenase
Gene
qdoI
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Performs the first step in the degradation of the flavonoid quercetin by a dioxygenase reaction. The enzyme catalyzes the cleavage of the O-heteroaromatic ring of the flavonol quercetin yielding the depside 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. This involves the remarkable dioxygenolytic cleavage of two carbon-carbon bonds.1 Publication
Catalytic activityi
- EC:1.13.11.24
Cofactori
Fe2+1 PublicationNote: Binds 2 Fe2+ ions per subunit.1 Publication
Kineticsi
- KM=3.8 µM for quercetin1 Publication
- Vmax=2 µmol/min/mg enzyme1 Publication
: quercetin degradation Pathwayi
This protein is involved in the pathway quercetin degradation, which is part of Flavonoid metabolism.View all proteins of this organism that are known to be involved in the pathway quercetin degradation and in Flavonoid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 62 | Iron 1 | 1 | |
Metal bindingi | 64 | Iron 1 | 1 | |
Metal bindingi | 69 | Iron 1 | 1 | |
Metal bindingi | 103 | Iron 1 | 1 | |
Metal bindingi | 234 | Iron 2 | 1 | |
Metal bindingi | 236 | Iron 2 | 1 | |
Metal bindingi | 241 | Iron 2 | 1 | |
Metal bindingi | 275 | Iron 2 | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- quercetin 2,3-dioxygenase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU39980-MONOMER |
BRENDAi | 1.13.11.24, 658 |
SABIO-RKi | P42106 |
UniPathwayi | UPA00724 |
Names & Taxonomyi
Protein namesi | Recommended name: Quercetin 2,3-dioxygenase (EC:1.13.11.24)Short name: Quercetinase Alternative name(s): Flavonol 2,4-dioxygenase |
Gene namesi | Name:qdoI Synonyms:yxaG Ordered Locus Names:BSU39980 ORF Names:S14G |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097135 | 1 – 337 | Quercetin 2,3-dioxygenaseAdd BLAST | 337 |
Proteomic databases
PaxDbi | P42106 |
PRIDEi | P42106 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
STRINGi | 224308.BSU39980 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P42106 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42106 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 148 | Cupin 1Add BLAST | 148 | |
Regioni | 177 – 333 | Cupin 2Add BLAST | 157 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1917, Bacteria |
InParanoidi | P42106 |
OMAi | DFLHVPA |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR013096, Cupin_2 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF07883, Cupin_2, 2 hits |
SUPFAMi | SSF51182, SSF51182, 1 hit |
i Sequence
Sequence statusi: Complete.
P42106-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTLCTHSLP KEKMPYLLRS GEGERYLFGR QVATVMANGR STGDLFEIVL
60 70 80 90 100
LSGGKGDAFP LHVHKDTHEG ILVLDGKLEL TLDGERYLLI SGDYANIPAG
110 120 130 140 150
TPHSYRMQSH RTRLVSYTMK GNVAHLYSVI GNPYDHAEHP PYASEEVSNE
160 170 180 190 200
RFAEAAAVAD IVFLDEAKPA CSAKLAELTE LPDGAVPYVL ESGEGDRLLT
210 220 230 240 250
GDQLHRIVAA QKNTDGQFIV VSSEGPKGDR IVDHYHEYHT ETFYCLEGQM
260 270 280 290 300
TMWTDGQEIQ LNPGDFLHVP ANTVHSYRLD SHYTKMVGVL VPGLFEPFFR
310 320 330
TLGDPYEGHI FPCEPQALRF DRILQNIEAL DLKVMKP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 160 | D → T in BAA21586 (PubMed:7584049).Curated | 1 | |
Sequence conflicti | 314 | E → K in BAA21586 (PubMed:7584049).Curated | 1 |
Mass spectrometryi
Molecular mass is 38658 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB005554 Genomic DNA Translation: BAA21586.1 AL009126 Genomic DNA Translation: CAB16035.2 |
PIRi | F70071 |
RefSeqi | NP_391878.2, NC_000964.3 WP_003243000.1, NZ_JNCM01000034.1 |
Genome annotation databases
EnsemblBacteriai | CAB16035; CAB16035; BSU39980 |
GeneIDi | 937689 |
KEGGi | bsu:BSU39980 |
PATRICi | fig|224308.179.peg.4324 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB005554 Genomic DNA Translation: BAA21586.1 AL009126 Genomic DNA Translation: CAB16035.2 |
PIRi | F70071 |
RefSeqi | NP_391878.2, NC_000964.3 WP_003243000.1, NZ_JNCM01000034.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Y3T | X-ray | 2.40 | A/B | 1-337 | [»] | |
2H0V | X-ray | 2.60 | A/B | 1-337 | [»] | |
SMRi | P42106 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU39980 |
Proteomic databases
PaxDbi | P42106 |
PRIDEi | P42106 |
Protocols and materials databases
DNASUi | 937689 |
Genome annotation databases
EnsemblBacteriai | CAB16035; CAB16035; BSU39980 |
GeneIDi | 937689 |
KEGGi | bsu:BSU39980 |
PATRICi | fig|224308.179.peg.4324 |
Phylogenomic databases
eggNOGi | COG1917, Bacteria |
InParanoidi | P42106 |
OMAi | DFLHVPA |
Enzyme and pathway databases
UniPathwayi | UPA00724 |
BioCyci | BSUB:BSU39980-MONOMER |
BRENDAi | 1.13.11.24, 658 |
SABIO-RKi | P42106 |
Miscellaneous databases
EvolutionaryTracei | P42106 |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR013096, Cupin_2 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF07883, Cupin_2, 2 hits |
SUPFAMi | SSF51182, SSF51182, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | QDOI_BACSU | |
Accessioni | P42106Primary (citable) accession number: P42106 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | July 28, 2009 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references