UniProtKB - P42032 (NUOH_RHOCA)
Protein
NADH-quinone oxidoreductase subunit H
Gene
nuoH
Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.
Catalytic activityi
GO - Molecular functioni
- oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Source: UniProtKB-UniRule
- quinone binding Source: UniProtKB-KW
Keywordsi
Molecular function | Translocase |
Ligand | NAD, Ubiquinone |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit HUniRule annotation (EC:7.1.1.-UniRule annotation)Alternative name(s): NADH dehydrogenase I subunit HUniRule annotation NDH-1 subunit HUniRule annotation |
Gene namesi | Name:nuoHUniRule annotation Synonyms:ndhA |
Organismi | Rhodobacter capsulatus (Rhodopseudomonas capsulata) |
Taxonomic identifieri | 1061 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter |
Subcellular locationi
- Cellular chromatophore membrane 2 Publications; Multi-pass membrane protein UniRule annotation2 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | Lumenal1 PublicationAdd BLAST | 15 | |
Transmembranei | 16 – 35 | Helical; Name=1CuratedAdd BLAST | 20 | |
Topological domaini | 36 – 86 | Cytoplasmic1 PublicationAdd BLAST | 51 | |
Transmembranei | 87 – 106 | Helical; Name=2CuratedAdd BLAST | 20 | |
Topological domaini | 107 – 110 | Lumenal1 Publication | 4 | |
Transmembranei | 111 – 130 | Helical; Name=3CuratedAdd BLAST | 20 | |
Topological domaini | 131 – 156 | Cytoplasmic1 PublicationAdd BLAST | 26 | |
Transmembranei | 157 – 176 | Helical; Name=4CuratedAdd BLAST | 20 | |
Topological domaini | 177 – 191 | Lumenal1 PublicationAdd BLAST | 15 | |
Transmembranei | 192 – 211 | Helical; Name=5CuratedAdd BLAST | 20 | |
Topological domaini | 212 – 245 | Cytoplasmic1 PublicationAdd BLAST | 34 | |
Transmembranei | 246 – 265 | Helical; Name=6CuratedAdd BLAST | 20 | |
Topological domaini | 266 – 276 | Lumenal1 PublicationAdd BLAST | 11 | |
Transmembranei | 277 – 296 | Helical; Name=7CuratedAdd BLAST | 20 | |
Topological domaini | 297 – 313 | Cytoplasmic1 PublicationAdd BLAST | 17 | |
Transmembranei | 314 – 333 | Helical; Name=8CuratedAdd BLAST | 20 | |
Topological domaini | 334 – 345 | Lumenal1 PublicationAdd BLAST | 12 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-UniRule
- plasma membrane-derived chromatophore membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
No functional NADH-quinone oxidoreductase complex. Cells lacking this gene have a nearly normal respiratory growth phenotype on lactate, however they are unable to perform anaerobic photosynthesis. It is suggested that in R.capsulatus this complex may function in reverse flow under physiological conditions.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000117531 | 1 – 345 | NADH-quinone oxidoreductase subunit HAdd BLAST | 345 |
Keywords - PTMi
QuinoneInteractioni
Subunit structurei
NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex (Probable).
CuratedFamily & Domainsi
Sequence similaritiesi
Belongs to the complex I subunit 1 family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
HAMAPi | MF_01350, NDH1_NuoH, 1 hit |
InterProi | View protein in InterPro IPR001694, NADH_UbQ_OxRdtase_su1/FPO IPR018086, NADH_UbQ_OxRdtase_su1_CS |
PANTHERi | PTHR11432, PTHR11432, 1 hit |
Pfami | View protein in Pfam PF00146, NADHdh, 1 hit |
PROSITEi | View protein in PROSITE PS00667, COMPLEX1_ND1_1, 1 hit PS00668, COMPLEX1_ND1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P42032-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADFWATSLG QTLILLAQGL GIIAFVMIGL LLLVWGDRKI WAAVQMRKGP
60 70 80 90 100
NVVGAFGLLQ SVADAAKYVF KEIVVPAGVD KPVYFLAPML SLVLALLAWV
110 120 130 140 150
VVPFNEGWVM ADINVAVLFV FAVSSLEVYG VIMGGWASNS KYPFLGSLRS
160 170 180 190 200
AAQMISYEVS MGLIIVGVII STGSMNLSAI VEAQRGDFGL LNWYWLPHLP
210 220 230 240 250
MVALFFISAL AETNRPPFDL PEAESELVAG FMVEYSSTPY LLFMAGEYIA
260 270 280 290 300
VWLMCALTSV LFFGGWLSPI PGVPDGVLWM VAKMAAVFFV FAMVKAIVPR
310 320 330 340
YRYDQLMRIG WKVFLPLSLA WVVVVAFLAK FEVLGGFWAR WSIGA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF029365 Genomic DNA Translation: AAC24997.1 |
PIRi | S22368 |
Genome annotation databases
GeneIDi | 31490409 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF029365 Genomic DNA Translation: AAC24997.1 |
PIRi | S22368 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
GeneIDi | 31490409 |
Family and domain databases
HAMAPi | MF_01350, NDH1_NuoH, 1 hit |
InterProi | View protein in InterPro IPR001694, NADH_UbQ_OxRdtase_su1/FPO IPR018086, NADH_UbQ_OxRdtase_su1_CS |
PANTHERi | PTHR11432, PTHR11432, 1 hit |
Pfami | View protein in Pfam PF00146, NADHdh, 1 hit |
PROSITEi | View protein in PROSITE PS00667, COMPLEX1_ND1_1, 1 hit PS00668, COMPLEX1_ND1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOH_RHOCA | |
Accessioni | P42032Primary (citable) accession number: P42032 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | August 12, 2020 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |