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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi119Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi123Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105 Respiratory electron transport
R-BTA-6799198 Complex I biogenesis

Protein family/group databases

TCDBi3.D.1.6.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
TYKY subunit
Gene namesi
Name:NDUFS8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Organism-specific databases

VGNCiVGNC:31973 NDUFS8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36Mitochondrion1 PublicationAdd BLAST36
ChainiPRO_000002001137 – 212NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialAdd BLAST176

Proteomic databases

PaxDbiP42028
PeptideAtlasiP42028
PRIDEiP42028

Expressioni

Gene expression databases

BgeeiENSBTAG00000012072

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.2 Publications

Protein-protein interaction databases

CORUMiP42028
DIPiDIP-38810N
IntActiP42028, 2 interactors
STRINGi9913.ENSBTAP00000016013

Structurei

3D structure databases

ProteinModelPortaliP42028
SMRiP42028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 1334Fe-4S ferredoxin-type 1Add BLAST30
Domaini143 – 1724Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256 Eukaryota
COG1143 LUCA
GeneTreeiENSGT00390000003049
HOGENOMiHOG000228289
HOVERGENiHBG006547
InParanoidiP42028
KOiK03941
OMAiEIARGMY
OrthoDBiEOG091G0OQD
TreeFamiTF105610

Family and domain databases

HAMAPiMF_01351 NDH1_NuoI, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR010226 NADH_quinone_OxRdtase_chainI
PfamiView protein in Pfam
PF12838 Fer4_7, 1 hit
TIGRFAMsiTIGR01971 NuoI, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM
60 70 80 90 100
KSVTDRAAQT LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AVCPAQAITI EAEPRADGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE
210
IAANIQADYL YR
Length:212
Mass (Da):23,896
Last modified:November 1, 1995 - v1
Checksum:i4D5F95E4C918FEF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129T → I in AAI09907 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58717 mRNA Translation: AAA30664.1
BC109906 mRNA Translation: AAI09907.1
PIRiA38409
RefSeqiNP_001289598.1, NM_001302669.1
XP_005227041.1, XM_005226984.2
UniGeneiBt.5483

Genome annotation databases

EnsembliENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072
GeneIDi287027
KEGGibta:287027

Similar proteinsi

Entry informationi

Entry nameiNDUS8_BOVIN
AccessioniPrimary (citable) accession number: P42028
Secondary accession number(s): Q32KV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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