UniProtKB - P41958 (CED9_CAEEL)
Apoptosis regulator ced-9
ced-9
Functioni
Plays a major role in programmed cell death (PCD, apoptosis) (PubMed:7907274, PubMed:10688797).
egl-1 binds to and directly inhibits the activity of ced-9, releasing the cell death activator ced-4 from a ced-9/ced-4 containing protein complex and allowing ced-4 to activate the cell-killing caspase ced-3 (PubMed:9024666, PubMed:9027313, PubMed:9604928, PubMed:12894216, PubMed:15383288, PubMed:16208361).
During larval development, required for the elimination of transient presynaptic components downstream of egl-1 and upstream of ced-4 and ced-3 apoptotic pathway (PubMed:26074078).
7 PublicationsGO - Molecular functioni
- GTPase activator activity Source: WormBase
- protein heterodimerization activity Source: GO_Central
- protein homodimerization activity Source: GO_Central
- protein sequestering activity Source: UniProtKB
GO - Biological processi
- actin filament depolymerization Source: UniProtKB
- apoptotic process Source: UniProtKB
- apoptotic process involved in development Source: UniProtKB
- autophagy of mitochondrion Source: WormBase
- defense response to Gram-negative bacterium Source: UniProtKB
- extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
- intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
- negative regulation of apoptotic process Source: WormBase
- negative regulation of execution phase of apoptosis Source: ComplexPortal
- negative regulation of intrinsic apoptotic signaling pathway Source: GO_Central
- negative regulation of programmed cell death Source: WormBase
- negative regulation of protein-containing complex assembly Source: UniProtKB
- positive regulation of apoptotic process Source: ComplexPortal
- positive regulation of brood size Source: UniProtKB
- positive regulation of embryonic development Source: UniProtKB
- positive regulation of fertilization Source: UniProtKB
- positive regulation of mitochondrial fusion Source: WormBase
- positive regulation of oviposition Source: UniProtKB
- positive regulation of synapse pruning Source: UniProtKB
- protein processing Source: UniProtKB
- regulation of synapse organization Source: UniProtKB
Keywordsi
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-CEL-9648002, RAS processing |
Protein family/group databases
TCDBi | 1.A.21.1.12, the bcl-2 (bcl-2) family |
Names & Taxonomyi
Protein namesi | Recommended name: Apoptosis regulator ced-9Alternative name(s): Cell death protein 9 |
Gene namesi | Name:ced-9 ORF Names:T07C4.8 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | T07C4.8 ; CE25104 ; WBGene00000423 ; ced-9 |
Subcellular locationi
Mitochondrion
- Mitochondrion membrane 2 Publications; Peripheral membrane protein 2 Publications
Other locations
- Perikaryon 1 Publication
- synapse 1 Publication
- Endomembrane system 2 Publications; Peripheral membrane protein
Note: Intracellular membranes, mitochondrial, and perinuclear region (PubMed:9027313, PubMed:10688797). Localizes to synapses of DD motor neurons (PubMed:26074078). Synaptic localization is dependent on axonal mitochondria (PubMed:26074078).1 Publication
Mitochondrion
- mitochondrial outer membrane Source: WormBase
- mitochondrion Source: WormBase
Other locations
- endomembrane system Source: UniProtKB-SubCell
- membrane Source: WormBase
- neuronal cell body Source: UniProtKB
- organelle membrane Source: WormBase
- perikaryon Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: WormBase
- presynapse Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Membrane, Mitochondrion, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 149 | Y → N in n1653; no effect on the interaction with ced-4. Normal elimination of presynaptic components in RME neurons in adults. 2 Publications | 1 | |
Mutagenesisi | 169 | G → E in n1950; gain of function mutant. No effect on the interaction with ced-4. Impaired elimination of presynaptic components in RME neurons in adults. Abnormal accumulation of F-actin at the non-eliminated transient synapses in DD neuron dorsal cord in L4 larvae. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000143097 | 1 – 280 | Apoptosis regulator ced-9Add BLAST | 280 |
Proteomic databases
EPDi | P41958 |
PaxDbi | P41958 |
PeptideAtlasi | P41958 |
PTM databases
iPTMneti | P41958 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | WBGene00000423, Expressed in multi-cellular organism and 6 other tissues |
Interactioni
Subunit structurei
Interacts with asymmetric homodimer ced-4; the interaction sequesters ced-4 (PubMed:9024666, PubMed:9027313, PubMed:15383288, PubMed:16208361).
Interacts with egl-1; the interaction results in ced-4 release (PubMed:9604928, PubMed:12894216, PubMed:15383288).
Interacts with dre-1; the interaction inhibits ced-9 activity, either directly or indirectly (PubMed:23431138).
Interacts with dct-1 (PubMed:11114722). May form a complex composed of ced-9, ced-4 and mac-1 (PubMed:10101135).
9 PublicationsBinary interactionsi
P41958
With | #Exp. | IntAct |
---|---|---|
ced-3 [P42573] | 4 | EBI-494110,EBI-494247 |
ced-4 [P30429] | 17 | EBI-494110,EBI-494118 |
ced-4 - isoform a [P30429-2] | 5 | EBI-494110,EBI-536271 |
egl-1 [O61667] | 7 | EBI-494110,EBI-495949 |
GO - Molecular functioni
- protein heterodimerization activity Source: GO_Central
- protein homodimerization activity Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 533094, 14 interactors |
ComplexPortali | CPX-1359, ced-4-ced-9-mac-1 complex CPX-398, ced-9-egl-1 complex CPX-399, ced-9-ced-4 complex |
DIPi | DIP-250N |
ELMi | P41958 |
IntActi | P41958, 4 interactors |
STRINGi | 6239.T07C4.8 |
Structurei
Secondary structure
3D structure databases
SMRi | P41958 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P41958 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 59 | DisorderedSequence analysisAdd BLAST | 27 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 80 – 99 | BH4PROSITE-ProRule annotationAdd BLAST | 20 | |
Motifi | 160 – 179 | BH1Sequence analysisAdd BLAST | 20 | |
Motifi | 213 – 229 | BH2CuratedAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 39 – 59 | Polar residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4728, Eukaryota |
GeneTreei | ENSGT01020000230347 |
HOGENOMi | CLU_1046733_0_0_1 |
InParanoidi | P41958 |
OMAi | YVEVVEC |
OrthoDBi | 1218929at2759 |
PhylomeDBi | P41958 |
Family and domain databases
CDDi | cd06845, Bcl-2_like, 1 hit |
Gene3Di | 1.10.437.10, 1 hit |
InterProi | View protein in InterPro IPR036834, Bcl-2-like_sf IPR026298, Bcl-2_fam IPR002475, Bcl2-like IPR020717, Bcl2_BH1_motif_CS IPR003093, Bcl2_BH4 |
PANTHERi | PTHR11256, PTHR11256, 1 hit |
Pfami | View protein in Pfam PF00452, Bcl-2, 1 hit PF02180, BH4, 1 hit |
SMARTi | View protein in SMART SM00337, BCL, 1 hit SM00265, BH4, 1 hit |
SUPFAMi | SSF56854, SSF56854, 1 hit |
PROSITEi | View protein in PROSITE PS50062, BCL2_FAMILY, 1 hit PS01080, BH1, 1 hit PS50063, BH4_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTRCTADNSL TNPAYRRRTM ATGEMKEFLG IKGTEPTDFG INSDAQDLPS
60 70 80 90 100
PSRQASTRRM SIGESIDGKI NDWEEPRLDI EGFVVDYFTH RIRQNGMEWF
110 120 130 140 150
GAPGLPCGVQ PEHEMMRVMG TIFEKKHAEN FETFCEQLLA VPRISFSLYQ
160 170 180 190 200
DVVRTVGNAQ TDQCPMSYGR LIGLISFGGF VAAKMMESVE LQGQVRNLFV
210 220 230 240 250
YTSLFIKTRI RNNWKEHNRS WDDFMTLGKQ MKEDYERAEA EKVGRRKQNR
260 270 280
RWSMIGAGVT AGAIGIVGVV VCGRMMFSLK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26545 Genomic DNA Translation: AAA20080.1 Z29443 Genomic DNA Translation: CAA82573.2 |
PIRi | A53189 H88578 |
RefSeqi | NP_499284.1, NM_066883.5 |
Genome annotation databases
EnsemblMetazoai | T07C4.8.1; T07C4.8.1; WBGene00000423 |
GeneIDi | 3565776 |
KEGGi | cel:CELE_T07C4.8 |
UCSCi | T07C4.8.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26545 Genomic DNA Translation: AAA20080.1 Z29443 Genomic DNA Translation: CAA82573.2 |
PIRi | A53189 H88578 |
RefSeqi | NP_499284.1, NM_066883.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OHU | X-ray | 2.03 | A/B | 68-242 | [»] | |
1TY4 | X-ray | 2.20 | A/B | 68-237 | [»] | |
2A5Y | X-ray | 2.60 | A | 48-251 | [»] | |
SMRi | P41958 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 533094, 14 interactors |
ComplexPortali | CPX-1359, ced-4-ced-9-mac-1 complex CPX-398, ced-9-egl-1 complex CPX-399, ced-9-ced-4 complex |
DIPi | DIP-250N |
ELMi | P41958 |
IntActi | P41958, 4 interactors |
STRINGi | 6239.T07C4.8 |
Protein family/group databases
TCDBi | 1.A.21.1.12, the bcl-2 (bcl-2) family |
PTM databases
iPTMneti | P41958 |
Proteomic databases
EPDi | P41958 |
PaxDbi | P41958 |
PeptideAtlasi | P41958 |
Genome annotation databases
EnsemblMetazoai | T07C4.8.1; T07C4.8.1; WBGene00000423 |
GeneIDi | 3565776 |
KEGGi | cel:CELE_T07C4.8 |
UCSCi | T07C4.8.1, c. elegans |
Organism-specific databases
CTDi | 3565776 |
WormBasei | T07C4.8 ; CE25104 ; WBGene00000423 ; ced-9 |
Phylogenomic databases
eggNOGi | KOG4728, Eukaryota |
GeneTreei | ENSGT01020000230347 |
HOGENOMi | CLU_1046733_0_0_1 |
InParanoidi | P41958 |
OMAi | YVEVVEC |
OrthoDBi | 1218929at2759 |
PhylomeDBi | P41958 |
Enzyme and pathway databases
Reactomei | R-CEL-9648002, RAS processing |
Miscellaneous databases
EvolutionaryTracei | P41958 |
PROi | PR:P41958 |
Gene expression databases
Bgeei | WBGene00000423, Expressed in multi-cellular organism and 6 other tissues |
Family and domain databases
CDDi | cd06845, Bcl-2_like, 1 hit |
Gene3Di | 1.10.437.10, 1 hit |
InterProi | View protein in InterPro IPR036834, Bcl-2-like_sf IPR026298, Bcl-2_fam IPR002475, Bcl2-like IPR020717, Bcl2_BH1_motif_CS IPR003093, Bcl2_BH4 |
PANTHERi | PTHR11256, PTHR11256, 1 hit |
Pfami | View protein in Pfam PF00452, Bcl-2, 1 hit PF02180, BH4, 1 hit |
SMARTi | View protein in SMART SM00337, BCL, 1 hit SM00265, BH4, 1 hit |
SUPFAMi | SSF56854, SSF56854, 1 hit |
PROSITEi | View protein in PROSITE PS50062, BCL2_FAMILY, 1 hit PS01080, BH1, 1 hit PS50063, BH4_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CED9_CAEEL | |
Accessioni | P41958Primary (citable) accession number: P41958 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families