UniProtKB - P41939 (IDHC_YEAST)
Protein
Isocitrate dehydrogenase [NADP] cytoplasmic
Gene
IDP2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
May function in the production of NADPH for fatty acid and sterol synthesis.
Miscellaneous
Present with 2620 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.1.1.42
Cofactori
Activity regulationi
By catabolite repression.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 77 | SubstrateBy similarity | 1 | |
Binding sitei | 82 | NADPBy similarity | 1 | |
Binding sitei | 109 | SubstrateBy similarity | 1 | |
Binding sitei | 132 | SubstrateBy similarity | 1 | |
Sitei | 139 | Critical for catalysisBy similarity | 1 | |
Sitei | 212 | Critical for catalysisBy similarity | 1 | |
Metal bindingi | 252 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 260 | NADPBy similarity | 1 | |
Metal bindingi | 275 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 328 | NADP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 75 – 77 | NADPBy similarity | 3 | |
Nucleotide bindingi | 310 – 315 | NADPBy similarity | 6 |
GO - Molecular functioni
- isocitrate dehydrogenase (NADP+) activity Source: SGD
- magnesium ion binding Source: InterPro
- NAD binding Source: InterPro
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-KW
- isocitrate metabolic process Source: SGD
- NADP metabolic process Source: GO_Central
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Manganese, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:YLR174W-MONOMER |
BRENDAi | 1.1.1.42, 984 |
Reactomei | R-SCE-389542, NADPH regeneration R-SCE-6798695, Neutrophil degranulation R-SCE-9033241, Peroxisomal protein import |
SABIO-RKi | P41939 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)Short name: IDH Alternative name(s): IDP NADP(+)-specific ICDH Oxalosuccinate decarboxylase |
Gene namesi | Name:IDP2 Ordered Locus Names:YLR174W ORF Names:L9470.12 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004164, IDP2 |
VEuPathDBi | FungiDB:YLR174W |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: SGD
Mitochondrion
- mitochondrion Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083587 | 1 – 412 | Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST | 412 |
Post-translational modificationi
The N-terminus is blocked.
Proteomic databases
MaxQBi | P41939 |
PaxDbi | P41939 |
PRIDEi | P41939 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 31445, 94 interactors |
DIPi | DIP-4252N |
IntActi | P41939, 2 interactors |
STRINGi | 4932.YLR174W |
Miscellaneous databases
RNActi | P41939, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 94 – 100 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family.Curated
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
GeneTreei | ENSGT00390000012547 |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | P41939 |
OMAi | EATLKYG |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P41939-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKIKVANPI VEMDGDEQTR IIWHLIRDKL VLPYLDVDLK YYDLSVEYRD
60 70 80 90 100
QTNDQVTVDS ATATLKYGVA VKCATITPDE ARVEEFHLKK MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE PIIIPRIPRL VPQWEKPIII GRHAFGDQYK ATDVIVPEEG
160 170 180 190 200
ELRLVYKSKS GTHDVDLKVF DYPEHGGVAM MMYNTTDSIE GFAKASFELA
210 220 230 240 250
IERKLPLYST TKNTILKKYD GKFKDVFEAM YARSYKEKFE SLGIWYEHRL
260 270 280 290 300
IDDMVAQMLK SKGGYIIAMK NYDGDVESDI VAQGFGSLGL MTSVLITPDG
310 320 330 340 350
KTFESEAAHG TVTRHFRQHQ QGKETSTNSI ASIFAWTRGI IQRGKLDNTP
360 370 380 390 400
DVVKFGQILE SATVNTVQED GIMTKDLALI LGKSERSAYV TTEEFIDAVE
410
SRLKKEFEAA AL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 – 27 | HLIR → SFNQ in AAA64516 (PubMed:8068643).Curated | 4 | |
Sequence conflicti | 68 | G → R in AAA64516 (PubMed:8068643).Curated | 1 | |
Sequence conflicti | 185 | T → N in AAA64516 (PubMed:8068643).Curated | 1 | |
Sequence conflicti | 232 – 239 | ARSYKEKF → LEVIKRSL in AAA64516 (PubMed:8068643).Curated | 8 | |
Sequence conflicti | 267 | I → M in AAA64516 (PubMed:8068643).Curated | 1 | |
Sequence conflicti | 306 – 307 | EA → DR in AAA64516 (PubMed:8068643).Curated | 2 | |
Sequence conflicti | 316 – 323 | FRQHQQGK → LTDYDKGR in AAA64516 (PubMed:8068643).Curated | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26312 Genomic DNA Translation: AAA64516.1 U17246 Genomic DNA Translation: AAB67464.1 BK006945 Genomic DNA Translation: DAA09494.1 |
PIRi | S51419 |
RefSeqi | NP_013275.1, NM_001182061.1 |
Genome annotation databases
EnsemblFungii | YLR174W_mRNA; YLR174W; YLR174W |
GeneIDi | 850871 |
KEGGi | sce:YLR174W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26312 Genomic DNA Translation: AAA64516.1 U17246 Genomic DNA Translation: AAB67464.1 BK006945 Genomic DNA Translation: DAA09494.1 |
PIRi | S51419 |
RefSeqi | NP_013275.1, NM_001182061.1 |
3D structure databases
SMRi | P41939 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31445, 94 interactors |
DIPi | DIP-4252N |
IntActi | P41939, 2 interactors |
STRINGi | 4932.YLR174W |
Proteomic databases
MaxQBi | P41939 |
PaxDbi | P41939 |
PRIDEi | P41939 |
Genome annotation databases
EnsemblFungii | YLR174W_mRNA; YLR174W; YLR174W |
GeneIDi | 850871 |
KEGGi | sce:YLR174W |
Organism-specific databases
SGDi | S000004164, IDP2 |
VEuPathDBi | FungiDB:YLR174W |
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
GeneTreei | ENSGT00390000012547 |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | P41939 |
OMAi | EATLKYG |
Enzyme and pathway databases
BioCyci | MetaCyc:YLR174W-MONOMER |
BRENDAi | 1.1.1.42, 984 |
Reactomei | R-SCE-389542, NADPH regeneration R-SCE-6798695, Neutrophil degranulation R-SCE-9033241, Peroxisomal protein import |
SABIO-RKi | P41939 |
Miscellaneous databases
PROi | PR:P41939 |
RNActi | P41939, protein |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IDHC_YEAST | |
Accessioni | P41939Primary (citable) accession number: P41939 Secondary accession number(s): D6VYH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - SIMILARITY comments
Index of protein domains and families