UniProtKB - P41895 (T2FA_YEAST)
Protein
Transcription initiation factor IIF subunit alpha
Gene
TFG1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation.1 Publication
Miscellaneous
Present with 1920 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- protein phosphatase activator activity Source: SGD
- RNA polymerase II general transcription initiation factor activity Source: GO_Central
- TFIIF-class transcription factor complex binding Source: GO_Central
GO - Biological processi
- dephosphorylation of RNA polymerase II C-terminal domain Source: SGD
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- positive regulation of transcription elongation from RNA polymerase II promoter Source: InterPro
- promoter clearance from RNA polymerase II promoter Source: SGD
- regulation of protein serine/threonine phosphatase activity Source: SGD
- RNA polymerase II preinitiation complex assembly Source: SGD
- transcriptional start site selection at RNA polymerase II promoter Source: SGD
- transcription elongation from RNA polymerase II promoter Source: SGD
- transcription initiation from RNA polymerase II promoter Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-SCE-113418, Formation of the Early Elongation Complex R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-72086, mRNA Capping R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-75955, RNA Polymerase II Transcription Elongation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-77075, RNA Pol II CTD phosphorylation and interaction with CE R-SCE-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor IIF subunit alphaShort name: TFIIF-alpha Alternative name(s): TFIIF large subunit Transcription factor G 105 kDa subunit Short name: P105 |
Gene namesi | Name:TFG1 Synonyms:SSU71 Ordered Locus Names:YGR186W ORF Names:G7526 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003418, TFG1 |
VEuPathDBi | FungiDB:YGR186W |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211234 | 1 – 735 | Transcription initiation factor IIF subunit alphaAdd BLAST | 735 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 198 | PhosphoserineCombined sources | 1 | |
Modified residuei | 200 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 515 | PhosphoserineCombined sources | 1 | |
Modified residuei | 560 | PhosphoserineCombined sources | 1 | |
Modified residuei | 562 | PhosphoserineCombined sources | 1 | |
Modified residuei | 571 | PhosphoserineCombined sources | 1 | |
Modified residuei | 655 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P41895 |
PaxDbi | P41895 |
PRIDEi | P41895 |
PTM databases
iPTMneti | P41895 |
Interactioni
Subunit structurei
TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.
GO - Molecular functioni
- TFIIF-class transcription factor complex binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 33438, 150 interactors |
ComplexPortali | CPX-1149, Transcription factor TFIIF complex |
DIPi | DIP-1151N |
IntActi | P41895, 16 interactors |
MINTi | P41895 |
STRINGi | 4932.YGR186W |
Miscellaneous databases
RNActi | P41895, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 455 – 580 | Asp/Glu-rich (acidic)Add BLAST | 126 |
Sequence similaritiesi
Belongs to the TFIIF alpha subunit family.Curated
Phylogenomic databases
eggNOGi | KOG2393, Eukaryota |
GeneTreei | ENSGT00440000038032 |
HOGENOMi | CLU_020322_0_0_1 |
InParanoidi | P41895 |
OMAi | ENEINPY |
Family and domain databases
InterProi | View protein in InterPro IPR008851, TFIIF-alpha IPR011039, TFIIF_interaction |
PANTHERi | PTHR13011, PTHR13011, 1 hit |
Pfami | View protein in Pfam PF05793, TFIIF_alpha, 1 hit |
SUPFAMi | SSF50916, SSF50916, 2 hits |
i Sequence
Sequence statusi: Complete.
P41895-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN
60 70 80 90 100
GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVKV KDEDPNEYNE
110 120 130 140 150
FPLRAIPKED LENMRTHLLK FQSKKKINPV TDFHLPVRLH RKDTRNLQFQ
160 170 180 190 200
LTRAEIVQRQ KEISEYKKKA EQERSTPNSG GMNKSGTVSL NNTVKDGSQT
210 220 230 240 250
PTVDSVTKDN TANGVNSSIP TVTGSSVPPA SPTTVSAVES NGLSNGSTSA
260 270 280 290 300
ANGLDGNAST ANLANGRPLV TKLEDAGPAE DPTKVGMVKY DGKEVTNEPE
310 320 330 340 350
FEEGTMDPLA DVAPDGGGRA KRGNLRRKTR QLKVLDENAK KLRFEEFYPW
360 370 380 390 400
VMEDFDGYNT WVGSYEAGNS DSYVLLSVED DGSFTMIPAD KVYKFTARNK
410 420 430 440 450
YATLTIDEAE KRMDKKSGEV PRWLMKHLDN IGTTTTRYDR TRRKLKAVAD
460 470 480 490 500
QQAMDEDDRD DNSEVELDYD EEFADDEEAP IIDGNEQENK ESEQRIKKEM
510 520 530 540 550
LQANAMGLRD EEAPSENEED ELFGEKKIDE DGERIKKALQ KTELAALYSS
560 570 580 590 600
DENEINPYLS ESDIENKENE SPVKKEEDSD TLSKSKRSSP KKQQKKATNA
610 620 630 640 650
HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS
660 670 680 690 700
NTVPSPIKQE EGLNSTVAER EETPAPTITE KDIIEAIGDG KVNIKEFGKF
710 720 730
IRRKYPGAEN KKLMFAIVKK LCRKVGNDHM ELKKE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 238 | V → I in AAA61640 (PubMed:7995524).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U13015 Genomic DNA Translation: AAA61640.1 U14527 Genomic DNA Translation: AAA68478.1 X99074 Genomic DNA Translation: CAA67530.1 Z72971 Genomic DNA Translation: CAA97212.1 BK006941 Genomic DNA Translation: DAA08280.1 |
PIRi | S64504 |
RefSeqi | NP_011702.1, NM_001181315.1 |
Genome annotation databases
EnsemblFungii | YGR186W_mRNA; YGR186W; YGR186W |
GeneIDi | 853098 |
KEGGi | sce:YGR186W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U13015 Genomic DNA Translation: AAA61640.1 U14527 Genomic DNA Translation: AAA68478.1 X99074 Genomic DNA Translation: CAA67530.1 Z72971 Genomic DNA Translation: CAA97212.1 BK006941 Genomic DNA Translation: DAA08280.1 |
PIRi | S64504 |
RefSeqi | NP_011702.1, NM_001181315.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5FMF | electron microscopy | 6.00 | U | 94-150 | [»] | |
U | 323-417 | [»] | ||||
5FYW | electron microscopy | 4.35 | Q | 1-735 | [»] | |
5FZ5 | electron microscopy | 8.80 | Q | 1-735 | [»] | |
5IP7 | X-ray | 3.52 | Q | 21-35 | [»] | |
5IP9 | X-ray | 3.90 | Q | 21-35 | [»] | |
5OQJ | electron microscopy | 4.70 | Q | 1-735 | [»] | |
5OQM | electron microscopy | 5.80 | Q | 1-735 | [»] | |
5SVA | electron microscopy | 15.30 | f | 1-735 | [»] | |
6GYK | electron microscopy | 5.10 | Q | 1-735 | [»] | |
6GYL | electron microscopy | 4.80 | Q | 1-735 | [»] | |
6GYM | electron microscopy | 6.70 | Q | 1-735 | [»] | |
BMRBi | P41895 | |||||
SMRi | P41895 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33438, 150 interactors |
ComplexPortali | CPX-1149, Transcription factor TFIIF complex |
DIPi | DIP-1151N |
IntActi | P41895, 16 interactors |
MINTi | P41895 |
STRINGi | 4932.YGR186W |
PTM databases
iPTMneti | P41895 |
Proteomic databases
MaxQBi | P41895 |
PaxDbi | P41895 |
PRIDEi | P41895 |
Genome annotation databases
EnsemblFungii | YGR186W_mRNA; YGR186W; YGR186W |
GeneIDi | 853098 |
KEGGi | sce:YGR186W |
Organism-specific databases
SGDi | S000003418, TFG1 |
VEuPathDBi | FungiDB:YGR186W |
Phylogenomic databases
eggNOGi | KOG2393, Eukaryota |
GeneTreei | ENSGT00440000038032 |
HOGENOMi | CLU_020322_0_0_1 |
InParanoidi | P41895 |
OMAi | ENEINPY |
Enzyme and pathway databases
Reactomei | R-SCE-113418, Formation of the Early Elongation Complex R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-72086, mRNA Capping R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-75955, RNA Polymerase II Transcription Elongation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-77075, RNA Pol II CTD phosphorylation and interaction with CE R-SCE-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
PROi | PR:P41895 |
RNActi | P41895, protein |
Family and domain databases
InterProi | View protein in InterPro IPR008851, TFIIF-alpha IPR011039, TFIIF_interaction |
PANTHERi | PTHR13011, PTHR13011, 1 hit |
Pfami | View protein in Pfam PF05793, TFIIF_alpha, 1 hit |
SUPFAMi | SSF50916, SSF50916, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | T2FA_YEAST | |
Accessioni | P41895Primary (citable) accession number: P41895 Secondary accession number(s): D6VUW9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families