UniProtKB - P41851 (GSPM_VIBCH)
Protein
Type II secretion system protein M
Gene
epsM
Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Functioni
Inner membrane component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Plays a role in the complex assembly and recruits EpsL resulting in a stable complex in the inner membrane. Provides thus a link between the energy-providing EpsE protein in the cytoplasm and the rest of the T2SS machinery.3 Publications
GO - Biological processi
- pathogenesis Source: TIGR
- protein secretion by the type II secretion system Source: InterPro
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Type II secretion system protein MShort name: T2SS protein M Alternative name(s): Cholera toxin secretion protein EpsM General secretion pathway protein M |
Gene namesi | Name:epsM Ordered Locus Names:VC_2724 |
Organismi | Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
Taxonomic identifieri | 243277 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane Curated; Single-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicBy similarityAdd BLAST | 22 | |
Transmembranei | 23 – 43 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 44 – 165 | PeriplasmicBy similarityAdd BLAST | 122 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
- type II protein secretion system complex Source: InterPro
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Absence of EpsM results in a decreased amount of EpsL in type II secretion system complexes.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207328 | 1 – 165 | Type II secretion system protein MAdd BLAST | 165 |
Interactioni
Subunit structurei
Type II secretion system is composed of four main components: the outer membrane complex, the inner membrane complex, the cytoplasmic secretion ATPase and the periplasm-spanning pseudopilus (By similarity). Forms homodimers (PubMed:15081815, PubMed:17921296).
Interacts with EpsL/GspL (PubMed:10322014, PubMed:10633109).
Interacts with EpsE/GspE (PubMed:10633109).
Interacts with EpsF/GspF (PubMed:10633109).
By similarity4 PublicationsBinary interactionsi
P41851
With | #Exp. | IntAct |
---|---|---|
epsL [P45782] | 2 | EBI-6400843,EBI-6400836 |
Protein-protein interaction databases
IntActi | P41851, 1 interactor |
STRINGi | 243277.VC_2724 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P41851 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P41851 |
Family & Domainsi
Domaini
The periplasmic region found in the proximity of the transmembrane appears to be critical for a stable interaction with EpsL.1 Publication
Sequence similaritiesi
Belongs to the GSP M family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3149, Bacteria |
HOGENOMi | CLU_118900_3_0_6 |
OMAi | PQGNKIQ |
Family and domain databases
DisProti | DP00725 |
InterProi | View protein in InterPro IPR007690, T2SS_GspM IPR023229, T2SS_M_periplasmic_sf |
Pfami | View protein in Pfam PF04612, T2SSM, 1 hit |
PIRSFi | PIRSF006291, GspM, 1 hit |
SUPFAMi | SSF103054, SSF103054, 1 hit |
i Sequence
Sequence statusi: Complete.
P41851-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKELLAPVQA WWRSVTPREQ KMVMGMGALT VLAIAYWGIW QPLSERTAQA
60 70 80 90 100
QARLQTEKQL LSWVSENAND IVTLRAQGGS DAPSDQPLNQ VITNSTRQFN
110 120 130 140 150
IELIRVQPRG EMMQVWIQPL PFSQLVSWIA YLQERQGVSV DAIDIDRGKV
160
NGVVEVKRLQ LKRGG
Sequence cautioni
The sequence AAF95864 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13660 Genomic DNA Translation: AAA60935.1 L33796 Genomic DNA Translation: AAA58794.1 AE003852 Genomic DNA Translation: AAF95864.1 Different initiation. |
PIRi | F82040 JN0847 |
RefSeqi | NP_232351.1, NC_002505.1 WP_000661339.1, NZ_LT906614.1 |
Genome annotation databases
EnsemblBacteriai | AAF95864; AAF95864; VC_2724 |
KEGGi | vch:VC2724 |
PATRICi | fig|243277.26.peg.2599 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13660 Genomic DNA Translation: AAA60935.1 L33796 Genomic DNA Translation: AAA58794.1 AE003852 Genomic DNA Translation: AAF95864.1 Different initiation. |
PIRi | F82040 JN0847 |
RefSeqi | NP_232351.1, NC_002505.1 WP_000661339.1, NZ_LT906614.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UV7 | X-ray | 1.70 | A/B | 65-165 | [»] | |
SMRi | P41851 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P41851, 1 interactor |
STRINGi | 243277.VC_2724 |
Protocols and materials databases
DNASUi | 2615552 |
Genome annotation databases
EnsemblBacteriai | AAF95864; AAF95864; VC_2724 |
KEGGi | vch:VC2724 |
PATRICi | fig|243277.26.peg.2599 |
Phylogenomic databases
eggNOGi | COG3149, Bacteria |
HOGENOMi | CLU_118900_3_0_6 |
OMAi | PQGNKIQ |
Miscellaneous databases
EvolutionaryTracei | P41851 |
Family and domain databases
DisProti | DP00725 |
InterProi | View protein in InterPro IPR007690, T2SS_GspM IPR023229, T2SS_M_periplasmic_sf |
Pfami | View protein in Pfam PF04612, T2SSM, 1 hit |
PIRSFi | PIRSF006291, GspM, 1 hit |
SUPFAMi | SSF103054, SSF103054, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSPM_VIBCH | |
Accessioni | P41851Primary (citable) accession number: P41851 Secondary accession number(s): Q9KNK8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families