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UniProtKB - P41835 (THI6_YEAST)
Protein
Thiamine biosynthetic bifunctional enzyme
Gene
THI6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential for thiamine biosynthesis. The kinase activity is involved in the salvage synthesis of TH-P from the thiazole.
Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).
By similarityCatalytic activityi
- 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ = CO2 + diphosphate + thiamine phosphateEC:2.5.1.3
- 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ = CO2 + diphosphate + thiamine phosphateEC:2.5.1.3
- 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H+ = diphosphate + thiamine phosphateEC:2.5.1.3
- EC:2.7.1.50
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole. This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Pathwayi: thiamine diphosphate biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole. This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 75 | HMP-PPBy similarity | 1 | |
Metal bindingi | 76 | MagnesiumBy similarity | 1 | |
Metal bindingi | 95 | MagnesiumBy similarity | 1 | |
Binding sitei | 114 | HMP-PPBy similarity | 1 | |
Binding sitei | 146 | HMP-PPBy similarity | 1 | |
Binding sitei | 181 | THZ-P; via amide nitrogenBy similarity | 1 | |
Binding sitei | 290 | 4-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity | 1 | |
Binding sitei | 365 | ATPBy similarity | 1 | |
Binding sitei | 415 | ATPBy similarity | 1 | |
Binding sitei | 462 | 4-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity | 1 | |
Active sitei | 465 | Proton acceptor; for hydroxyethylthiazole kinase activityBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- hydroxyethylthiazole kinase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
- thiamine-phosphate diphosphorylase activity Source: SGD
GO - Biological processi
- thiamine biosynthetic process Source: SGD
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Transferase |
Biological process | Thiamine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00060;UER00139 UPA00060;UER00141 |
Names & Taxonomyi
Protein namesi | Recommended name: Thiamine biosynthetic bifunctional enzymeIncluding the following 2 domains: Alternative name(s): Thiamine-phosphate pyrophosphorylase Short name: TMP pyrophosphorylase Short name: TMP-PPase Hydroxyethylthiazole kinase (EC:2.7.1.50) Alternative name(s): 4-methyl-5-beta-hydroxyethylthiazole kinase Short name: TH kinase Short name: THZ kinase |
Gene namesi | Name:THI6 Ordered Locus Names:YPL214C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006135, THI6 |
VEuPathDBi | FungiDB:YPL214C |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000157090 | 1 – 540 | Thiamine biosynthetic bifunctional enzymeAdd BLAST | 540 |
Proteomic databases
MaxQBi | P41835 |
PaxDbi | P41835 |
PRIDEi | P41835 |
Interactioni
Subunit structurei
Homooctamer.
Protein-protein interaction databases
BioGRIDi | 35971, 115 interactors |
DIPi | DIP-1666N |
IntActi | P41835, 1 interactor |
MINTi | P41835 |
STRINGi | 4932.YPL214C |
Miscellaneous databases
RNActi | P41835, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 238 | Thiamine-phosphate synthaseAdd BLAST | 238 | |
Regioni | 43 – 47 | HMP-PP bindingBy similarity | 5 | |
Regioni | 143 – 145 | THZ-P bindingBy similarity | 3 | |
Regioni | 209 – 210 | THZ-P bindingBy similarity | 2 | |
Regioni | 239 – 540 | Hydroxyethylthiazole kinaseAdd BLAST | 302 |
Sequence similaritiesi
In the N-terminal section; belongs to the thiamine-phosphate synthase family.Curated
In the C-terminal section; belongs to the Thz kinase family.Curated
Phylogenomic databases
eggNOGi | ENOG502QS2M, Eukaryota |
HOGENOMi | CLU_019943_1_1_1 |
InParanoidi | P41835 |
OMAi | HQNFGAN |
Family and domain databases
CDDi | cd01170, THZ_kinase, 1 hit cd00564, TMP_TenI, 1 hit |
Gene3Di | 3.20.20.70, 1 hit 3.40.1190.20, 1 hit |
HAMAPi | MF_00097, TMP_synthase, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR000417, Hyethyz_kinase IPR029056, Ribokinase-like IPR036206, ThiamineP_synth_sf IPR022998, ThiamineP_synth_TenI IPR034291, TMP_synthase |
Pfami | View protein in Pfam PF02110, HK, 1 hit PF02581, TMP-TENI, 1 hit |
PRINTSi | PR01099, HYETHTZKNASE |
SUPFAMi | SSF51391, SSF51391, 1 hit SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00693, thiE, 1 hit TIGR00694, thiM, 1 hit |
i Sequence
Sequence statusi: Complete.
P41835-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVFTKEEVDY SLYLVTDSTM LPPGTTLCSQ VEAGLKNGVT LVQIREKDIE
60 70 80 90 100
TKNFVAEALE VQKICKKYNV PLIINDRIDV AMAIDADGVH VGQDDMPIPM
110 120 130 140 150
VRKLLGPSKI LGWSVGKPSE VETLAKWGPD MVDYIGVGTL FPTSTKKNPK
160 170 180 190 200
KSPMGPQGAI AILDALEEFK ATWCRTVGIG GLHPDNIQRV LCQCVASNGK
210 220 230 240 250
RSLDGISLVS DIMAAPDACA ATKRLRGLLD ATRYQFVECE LNNTFPTTTS
260 270 280 290 300
IQNVISQVSN NRPLVQHITN KVHQNFGANV TLALGSSPIM SEIESEVSEL
310 320 330 340 350
ARIPNASLLL NTGSVAPIEM LKAAINAYNE VNRPITFDPV GYSATETRLC
360 370 380 390 400
LNNTLLTYGQ FACIKGNCSE ILSLAKLNNH KMKGVDSSSG KTNIDTLVRA
410 420 430 440 450
TQIVAFQYRT VAVCTGEFDC VADGTFGGEY KLSSGTEGIT AEDLPCVIIE
460 470 480 490 500
DGPIPIMGDI TASGCSLGST IASFIGGLDS TGKLFDAVVG AVLLYKSAGK
510 520 530 540
LASTRCQGSG SFHVELIDAL YQLFHENKPE KWSASLKKFK
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 370 | E → K in THI6-3; possesses TMP-PPASE activity only. | 1 | |
Natural varianti | 476 | G → D in THI6-2; both enzyme activities greatly decreased. | 1 | |
Natural varianti | 510 | G → D in THI6-1; both enzyme activities greatly decreased. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31908 Genomic DNA Translation: BAA06703.1 Z73570 Genomic DNA Translation: CAA97929.1 BK006949 Genomic DNA Translation: DAA11222.1 |
PIRi | A55145 |
RefSeqi | NP_015110.1, NM_001184028.1 |
Genome annotation databases
EnsemblFungii | YPL214C_mRNA; YPL214C; YPL214C |
GeneIDi | 855887 |
KEGGi | sce:YPL214C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31908 Genomic DNA Translation: BAA06703.1 Z73570 Genomic DNA Translation: CAA97929.1 BK006949 Genomic DNA Translation: DAA11222.1 |
PIRi | A55145 |
RefSeqi | NP_015110.1, NM_001184028.1 |
3D structure databases
SMRi | P41835 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35971, 115 interactors |
DIPi | DIP-1666N |
IntActi | P41835, 1 interactor |
MINTi | P41835 |
STRINGi | 4932.YPL214C |
Proteomic databases
MaxQBi | P41835 |
PaxDbi | P41835 |
PRIDEi | P41835 |
Genome annotation databases
EnsemblFungii | YPL214C_mRNA; YPL214C; YPL214C |
GeneIDi | 855887 |
KEGGi | sce:YPL214C |
Organism-specific databases
SGDi | S000006135, THI6 |
VEuPathDBi | FungiDB:YPL214C |
Phylogenomic databases
eggNOGi | ENOG502QS2M, Eukaryota |
HOGENOMi | CLU_019943_1_1_1 |
InParanoidi | P41835 |
OMAi | HQNFGAN |
Enzyme and pathway databases
UniPathwayi | UPA00060;UER00139 UPA00060;UER00141 |
Miscellaneous databases
PROi | PR:P41835 |
RNActi | P41835, protein |
Family and domain databases
CDDi | cd01170, THZ_kinase, 1 hit cd00564, TMP_TenI, 1 hit |
Gene3Di | 3.20.20.70, 1 hit 3.40.1190.20, 1 hit |
HAMAPi | MF_00097, TMP_synthase, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR000417, Hyethyz_kinase IPR029056, Ribokinase-like IPR036206, ThiamineP_synth_sf IPR022998, ThiamineP_synth_TenI IPR034291, TMP_synthase |
Pfami | View protein in Pfam PF02110, HK, 1 hit PF02581, TMP-TENI, 1 hit |
PRINTSi | PR01099, HYETHTZKNASE |
SUPFAMi | SSF51391, SSF51391, 1 hit SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00693, thiE, 1 hit TIGR00694, thiM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | THI6_YEAST | |
Accessioni | P41835Primary (citable) accession number: P41835 Secondary accession number(s): D6W3F6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families