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UniProtKB - P41819 (DIM1_YEAST)
Protein
Dimethyladenosine transferase
Gene
DIM1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.
Catalytic activityi
- adenosine1779/adenosine1780 in 18S rRNA + 4 S-adenosyl-L-methionine = 4 H+ + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA + 4 S-adenosyl-L-homocysteineEC:2.1.1.183
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 39 | S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation | 1 | |
Binding sitei | 64 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 85 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 113 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 128 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
- RNA binding Source: UniProtKB-KW
- rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: SGD
GO - Biological processi
- maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- rRNA methylation Source: GO_Central
- rRNA modification Source: SGD
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | Ribosome biogenesis, rRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Dimethyladenosine transferase (EC:2.1.1.183)Alternative name(s): 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase 18S rRNA dimethylase S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase |
Gene namesi | Name:DIM1 Ordered Locus Names:YPL266W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006187, DIM1 |
VEuPathDBi | FungiDB:YPL266W |
Subcellular locationi
Mitochondrion
- mitochondrial matrix Source: GO_Central
Nucleus
- nucleolus Source: UniProtKB-SubCell
Other locations
- 90S preribosome Source: SGD
- preribosome, small subunit precursor Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101464 | 1 – 318 | Dimethyladenosine transferaseAdd BLAST | 318 |
Proteomic databases
MaxQBi | P41819 |
PaxDbi | P41819 |
PRIDEi | P41819 |
PTM databases
iPTMneti | P41819 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35947, 291 interactors |
DIPi | DIP-3924N |
IntActi | P41819, 20 interactors |
STRINGi | 4932.YPL266W |
Miscellaneous databases
RNActi | P41819, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P41819 |
SMRi | P41819 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0820, Eukaryota |
GeneTreei | ENSGT00950000183142 |
HOGENOMi | CLU_041220_2_0_1 |
InParanoidi | P41819 |
OMAi | VNAQMWA |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR001737, KsgA/Erm IPR020596, rRNA_Ade_Mease_Trfase_CS IPR020598, rRNA_Ade_methylase_Trfase_N IPR011530, rRNA_adenine_dimethylase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11727, PTHR11727, 1 hit |
Pfami | View protein in Pfam PF00398, RrnaAD, 1 hit |
SMARTi | View protein in SMART SM00650, rADc, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00755, ksgA, 1 hit |
PROSITEi | View protein in PROSITE PS01131, RRNA_A_DIMETH, 1 hit PS51689, SAM_RNA_A_N6_MT, 1 hit |
i Sequence
Sequence statusi: Complete.
P41819-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD
60 70 80 90 100
KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPRM AAELTKRVRG
110 120 130 140 150
TPVEKKLEIM LGDFMKTELP YFDICISNTP YQISSPLVFK LINQPRPPRV
160 170 180 190 200
SILMFQREFA LRLLARPGDS LYCRLSANVQ MWANVTHIMK VGKNNFRPPP
210 220 230 240 250
QVESSVVRLE IKNPRPQVDY NEWDGLLRIV FVRKNRTISA GFKSTTVMDI
260 270 280 290 300
LEKNYKTFLA MNNEMVDDTK GSMHDVVKEK IDTVLKETDL GDKRAGKCDQ
310
NDFLRLLYAF HQVGIHFS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26480 Genomic DNA Translation: AAA57357.1 Z73622 Genomic DNA Translation: CAA98001.1 BK006949 Genomic DNA Translation: DAA11170.1 |
PIRi | S47985 |
RefSeqi | NP_015057.1, NM_001184080.1 |
Genome annotation databases
EnsemblFungii | YPL266W_mRNA; YPL266W; YPL266W |
GeneIDi | 855862 |
KEGGi | sce:YPL266W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26480 Genomic DNA Translation: AAA57357.1 Z73622 Genomic DNA Translation: CAA98001.1 BK006949 Genomic DNA Translation: DAA11170.1 |
PIRi | S47985 |
RefSeqi | NP_015057.1, NM_001184080.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6RBD | electron microscopy | 3.47 | y | 1-318 | [»] | |
6ZQD | electron microscopy | 3.80 | JL | 1-318 | [»] | |
6ZQE | electron microscopy | 7.10 | JL | 1-318 | [»] | |
6ZQF | electron microscopy | 4.90 | JL | 1-318 | [»] | |
6ZQG | electron microscopy | 3.50 | JL | 1-318 | [»] | |
7AJU | electron microscopy | 3.80 | JL | 1-318 | [»] | |
AlphaFoldDBi | P41819 | |||||
SMRi | P41819 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35947, 291 interactors |
DIPi | DIP-3924N |
IntActi | P41819, 20 interactors |
STRINGi | 4932.YPL266W |
PTM databases
iPTMneti | P41819 |
Proteomic databases
MaxQBi | P41819 |
PaxDbi | P41819 |
PRIDEi | P41819 |
Genome annotation databases
EnsemblFungii | YPL266W_mRNA; YPL266W; YPL266W |
GeneIDi | 855862 |
KEGGi | sce:YPL266W |
Organism-specific databases
SGDi | S000006187, DIM1 |
VEuPathDBi | FungiDB:YPL266W |
Phylogenomic databases
eggNOGi | KOG0820, Eukaryota |
GeneTreei | ENSGT00950000183142 |
HOGENOMi | CLU_041220_2_0_1 |
InParanoidi | P41819 |
OMAi | VNAQMWA |
Miscellaneous databases
PROi | PR:P41819 |
RNActi | P41819, protein |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR001737, KsgA/Erm IPR020596, rRNA_Ade_Mease_Trfase_CS IPR020598, rRNA_Ade_methylase_Trfase_N IPR011530, rRNA_adenine_dimethylase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11727, PTHR11727, 1 hit |
Pfami | View protein in Pfam PF00398, RrnaAD, 1 hit |
SMARTi | View protein in SMART SM00650, rADc, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00755, ksgA, 1 hit |
PROSITEi | View protein in PROSITE PS01131, RRNA_A_DIMETH, 1 hit PS51689, SAM_RNA_A_N6_MT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DIM1_YEAST | |
Accessioni | P41819Primary (citable) accession number: P41819 Secondary accession number(s): D6W3A4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families