UniProtKB - P41811 (COPB2_YEAST)
Coatomer subunit beta'
SEC27
Functioni
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.
1 PublicationMiscellaneous
GO - Molecular functioni
- structural molecule activity Source: InterPro
- ubiquitin binding Source: SGD
GO - Biological processi
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: SGD
- intracellular mRNA localization Source: SGD
- intracellular protein transport Source: GO_Central
- intra-Golgi vesicle-mediated transport Source: GO_Central
- late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: SGD
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-6807878, COPI-mediated anterograde transport R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Names & Taxonomyi
Protein namesi | Recommended name: Coatomer subunit beta'Alternative name(s): Beta'-coat protein Short name: Beta'-COP |
Gene namesi | Name:SEC27 Ordered Locus Names:YGL137W ORF Names:G2827 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003105, SEC27 |
VEuPathDBi | FungiDB:YGL137W |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- COPI-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.By similarity
Golgi apparatus
- COPI vesicle coat Source: SGD
- Golgi membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050918 | 1 – 889 | Coatomer subunit beta'Add BLAST | 889 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 326 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P41811 |
PaxDbi | P41811 |
PRIDEi | P41811 |
PTM databases
iPTMneti | P41811 |
Interactioni
Subunit structurei
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.
Interacts with the ESCRT-0 subunit VPS27.
1 PublicationBinary interactionsi
P41811
With | #Exp. | IntAct |
---|---|---|
COP1 [P53622] | 17 | EBI-4898,EBI-4860 |
HSP82 [P02829] | 2 | EBI-4898,EBI-8659 |
SEC28 [P40509] | 4 | EBI-4898,EBI-4884 |
GO - Molecular functioni
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 33115, 696 interactors |
ComplexPortali | CPX-1652, COPI vesicle coat complex |
DIPi | DIP-6468N |
IntActi | P41811, 162 interactors |
MINTi | P41811 |
STRINGi | 4932.YGL137W |
Miscellaneous databases
RNActi | P41811, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P41811 |
SMRi | P41811 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 11 – 41 | WD 1Add BLAST | 31 | |
Repeati | 53 – 83 | WD 2Add BLAST | 31 | |
Repeati | 95 – 125 | WD 3Add BLAST | 31 | |
Repeati | 138 – 169 | WD 4Add BLAST | 32 | |
Repeati | 182 – 214 | WD 5Add BLAST | 33 | |
Repeati | 226 – 256 | WD 6Add BLAST | 31 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 806 – 889 | DisorderedSequence analysisAdd BLAST | 84 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 832 – 867 | Basic and acidic residuesSequence analysisAdd BLAST | 36 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0276, Eukaryota |
GeneTreei | ENSGT00900000141083 |
HOGENOMi | CLU_005507_1_0_1 |
OMAi | NVFWNES |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR006692, Coatomer_WD-assoc_reg IPR016453, COPB2 IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF04053, Coatomer_WDAD, 1 hit PF00400, WD40, 4 hits |
PIRSFi | PIRSF005567, Coatomer_beta'_subunit, 1 hit |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 2 hits |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS
60 70 80 90 100
IQVTETPVRA GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI
110 120 130 140 150
RSIAVHPTKP YVLSGSDDLT VKLWNWENNW ALEQTFEGHE HFVMCVAFNP
160 170 180 190 200
KDPSTFASGC LDRTVKVWSL GQSTPNFTLT TGQERGVNYV DYYPLPDKPY
210 220 230 240 250
MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL PIIISGSEDG
260 270 280 290 300
TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL
310 320 330 340 350
GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK
360 370 380 390 400
ELGSVDVFPQ SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW
410 420 430 440 450
GPDSNSYALI DETGQIKYYK NFKEVTSWSV PMHSAIDRLF SGALLGVKSD
460 470 480 490 500
GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG ELVMIVNTNS NGDEASGYTL
510 520 530 540 550
LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK WVGDVFIFTT
560 570 580 590 600
ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS
610 620 630 640 650
LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN
660 670 680 690 700
ISPDQDQKFE LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA
710 720 730 740 750
VEAFTNAHDL ESLFLLHSSF NNKEGLVTLA KDAERAGKFN LAFNAYWIAG
760 770 780 790 800
DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD AVNDIVTKWK ENLILNGKNT
810 820 830 840 850
VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE AEVEDSEFKE
860 870 880
SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11237 Genomic DNA Translation: AAA61711.1 X92670 Genomic DNA Translation: CAA63359.1 Z72659 Genomic DNA Translation: CAA96848.1 BK006941 Genomic DNA Translation: DAA07973.1 |
PIRi | B55123 |
RefSeqi | NP_011378.1, NM_001181002.1 |
Genome annotation databases
EnsemblFungii | YGL137W_mRNA; YGL137W; YGL137W |
GeneIDi | 852740 |
KEGGi | sce:YGL137W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11237 Genomic DNA Translation: AAA61711.1 X92670 Genomic DNA Translation: CAA63359.1 Z72659 Genomic DNA Translation: CAA96848.1 BK006941 Genomic DNA Translation: DAA07973.1 |
PIRi | B55123 |
RefSeqi | NP_011378.1, NM_001181002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YNN | X-ray | 1.78 | A | 1-304 | [»] | |
2YNO | X-ray | 1.80 | A/B | 1-304 | [»] | |
2YNP | X-ray | 2.96 | A | 1-604 | [»] | |
4J73 | X-ray | 1.44 | A | 1-301 | [»] | |
4J77 | X-ray | 1.76 | A/B | 1-301 | [»] | |
4J78 | X-ray | 1.48 | A | 1-301 | [»] | |
4J79 | X-ray | 1.56 | A | 1-300 | [»] | |
4J81 | X-ray | 1.74 | A/B | 1-301 | [»] | |
4J82 | X-ray | 1.46 | A/B | 1-301 | [»] | |
4J84 | X-ray | 1.47 | A/B | 1-301 | [»] | |
4J86 | X-ray | 1.48 | A/B | 1-301 | [»] | |
AlphaFoldDBi | P41811 | |||||
SMRi | P41811 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33115, 696 interactors |
ComplexPortali | CPX-1652, COPI vesicle coat complex |
DIPi | DIP-6468N |
IntActi | P41811, 162 interactors |
MINTi | P41811 |
STRINGi | 4932.YGL137W |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
PTM databases
iPTMneti | P41811 |
Proteomic databases
MaxQBi | P41811 |
PaxDbi | P41811 |
PRIDEi | P41811 |
Genome annotation databases
EnsemblFungii | YGL137W_mRNA; YGL137W; YGL137W |
GeneIDi | 852740 |
KEGGi | sce:YGL137W |
Organism-specific databases
SGDi | S000003105, SEC27 |
VEuPathDBi | FungiDB:YGL137W |
Phylogenomic databases
eggNOGi | KOG0276, Eukaryota |
GeneTreei | ENSGT00900000141083 |
HOGENOMi | CLU_005507_1_0_1 |
OMAi | NVFWNES |
Enzyme and pathway databases
Reactomei | R-SCE-6807878, COPI-mediated anterograde transport R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
PROi | PR:P41811 |
RNActi | P41811, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR006692, Coatomer_WD-assoc_reg IPR016453, COPB2 IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF04053, Coatomer_WDAD, 1 hit PF00400, WD40, 4 hits |
PIRSFi | PIRSF005567, Coatomer_beta'_subunit, 1 hit |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 2 hits |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | COPB2_YEAST | |
Accessioni | P41811Primary (citable) accession number: P41811 Secondary accession number(s): D6VU12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families