Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-B-cell leukemia transcription factor 1

Gene

Pbx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the cAMP-dependent regulation of CYP17 gene expression via its cAMP-regulatory sequence (CRS1) 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Probably in complex with MEIS2, is involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, it is required for spleen development through a mechanism that involves CDKN2B repression.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi233 – 295Homeobox; TALE-typePROSITE-ProRule annotationAdd BLAST63

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: MGI

GO - Biological processi

  • adrenal gland development Source: MGI
  • animal organ morphogenesis Source: MGI
  • anterior/posterior pattern specification Source: MGI
  • branching involved in ureteric bud morphogenesis Source: MGI
  • embryonic hemopoiesis Source: MGI
  • embryonic limb morphogenesis Source: MGI
  • embryonic organ development Source: MGI
  • embryonic skeletal system development Source: MGI
  • negative regulation of DNA binding transcription factor activity Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of G2/M transition of mitotic cell cycle Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • proximal/distal pattern formation Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of ossification Source: MGI
  • sex differentiation Source: UniProtKB-KW
  • spleen development Source: MGI
  • steroid biosynthetic process Source: UniProtKB-KW
  • thymus development Source: MGI
  • urogenital system development Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Sexual differentiation, Steroidogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-B-cell leukemia transcription factor 1
Alternative name(s):
Homeobox protein PBX1
Gene namesi
Name:Pbx1
Synonyms:Pbx-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97495 Pbx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000492361 – 430Pre-B-cell leukemia transcription factor 1Add BLAST430

Proteomic databases

MaxQBiP41778
PaxDbiP41778
PRIDEiP41778

PTM databases

iPTMnetiP41778
PhosphoSitePlusiP41778

Expressioni

Tissue specificityi

Widely distributed in steroidogenic and non-steroidogenic cells.

Gene expression databases

BgeeiENSMUSG00000052534
CleanExiMM_PBX1
ExpressionAtlasiP41778 baseline and differential
GenevisibleiP41778 MM

Interactioni

Subunit structurei

Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1 and TLX1. Isoform PBX1a interacts with MEIS2 isoform Meis2D, SP1, SP3 and KLF4. Isoform PBX1b is part of a PDX1:PBX1b:MEIS2b complex; PBX1b recruits Meis2B to the complex. Interacts with FOXC1 (By similarity).By similarity7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi202037, 23 interactors
CORUMiP41778
DIPiDIP-6107N
IntActiP41778, 6 interactors
MINTiP41778
STRINGi10090.ENSMUSP00000135516

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi236 – 240Combined sources5
Turni241 – 243Combined sources3
Helixi244 – 254Combined sources11
Turni255 – 257Combined sources3
Helixi263 – 273Combined sources11
Helixi277 – 287Combined sources11
Turni288 – 290Combined sources3
Helixi295 – 307Combined sources13

3D structure databases

ProteinModelPortaliP41778
SMRiP41778
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41778

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi127 – 135Poly-Ala9

Sequence similaritiesi

Belongs to the TALE/PBX homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0774 Eukaryota
ENOG410XRVF LUCA
GeneTreeiENSGT00390000016426
HOGENOMiHOG000266972
HOVERGENiHBG000122
InParanoidiP41778
KOiK09355
OMAiSTPNSAX
OrthoDBiEOG091G10TD
PhylomeDBiP41778
TreeFamiTF314340

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR005542 PBX
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF03792 PBC, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform PBX1a (identifier: P41778-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI
60 70 80 90 100
MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP
110 120 130 140 150
TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY
160 170 180 190 200
RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM NLLREQSRTR PISPKEIERM
210 220 230 240 250
VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF NKQATEILNE
260 270 280 290 300
YFYSHLSNPY PSEEAKEELA KKCGITVSQV SNWFGNKRIR YKKNIGKFQE
310 320 330 340 350
EANIYAAKTA VTATNVSAHG SQANSPSTPN SAGSSSSFNM SNSGDLFMSV
360 370 380 390 400
QSLNGDSYQG AQVGANVQSQ VDTLRHVISQ TGGYSDGLAA SQMYSPQGIS
410 420 430
ANGGWQDATT PSSVTSPTEG PGSVHSDTSN
Length:430
Mass (Da):46,626
Last modified:February 21, 2002 - v2
Checksum:iAD3FFACBC5A9E715
GO
Isoform PBX1b (identifier: P41778-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-347: SSSSFNMSNSGDLF → GYPSPCYQPDRRIQ
     348-430: Missing.

Show »
Length:347
Mass (Da):38,427
Checksum:iC4A2BDDD4A410C20
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002273334 – 347SSSSF…SGDLF → GYPSPCYQPDRRIQ in isoform PBX1b. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_002274348 – 430Missing in isoform PBX1b. 1 PublicationAdd BLAST83

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27453 mRNA Translation: AAA21832.1
AF020196 mRNA Translation: AAB71191.1
BC058390 mRNA Translation: AAH58390.1
CCDSiCCDS15461.1 [P41778-1]
CCDS15462.1 [P41778-2]
PIRiA54863
RefSeqiNP_001278437.1, NM_001291508.1 [P41778-2]
NP_032809.1, NM_008783.3 [P41778-2]
NP_899198.1, NM_183355.3 [P41778-1]
UniGeneiMm.43358
Mm.440114

Genome annotation databases

EnsembliENSMUST00000072863; ENSMUSP00000072640; ENSMUSG00000052534 [P41778-2]
ENSMUST00000176540; ENSMUSP00000135516; ENSMUSG00000052534 [P41778-1]
ENSMUST00000176790; ENSMUSP00000134925; ENSMUSG00000052534 [P41778-2]
ENSMUST00000188912; ENSMUSP00000140606; ENSMUSG00000052534 [P41778-2]
GeneIDi18514
KEGGimmu:18514
UCSCiuc007dle.2 mouse [P41778-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPBX1_MOUSE
AccessioniPrimary (citable) accession number: P41778
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 21, 2002
Last modified: July 18, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health