UniProtKB - P41778 (PBX1_MOUSE)
Pre-B-cell leukemia transcription factor 1
Pbx1
Functioni
Transcription factor which binds the DNA sequence 5'-TGATTGAT-3' as part of a heterodimer with HOX proteins such as HOXA1, HOXA5, HOXB7 and HOXB8 (By similarity).
Binds the DNA sequence 5'-TGATTGAC-3' in complex with a nuclear factor which is not a class I HOX protein (By similarity).
Has also been shown to bind the DNA sequence 5'-ATCAATCAA-3' cooperatively with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4 (PubMed:7791786).
Acts as a transcriptional activator of PF4 in complex with MEIS1 (By similarity).
Also activates transcription of SOX3 in complex with MEIS1 by binding to the 5'-TGATTGAC-3' consensus sequence (PubMed:19799567).
In natural killer cells, binds to the NFIL3 promoter and acts as a transcriptional activator of NFIL3, promoting natural killer cell development (PubMed:32190943).
Plays a role in the cAMP-dependent regulation of CYP17A1 gene expression via its cAMP-regulatory sequence (CRS1) (PubMed:7913464).
Probably in complex with MEIS2, is involved in transcriptional regulation by KLF4 (By similarity).
Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B (PubMed:22560297).
Together with NKX2-5, required for spleen development through a mechanism that involves CDKN2B repression (PubMed:22560297).
By similarity5 PublicationsAs part of a PDX1:PBX1b:MEIS2B complex in pancreatic acinar cells, is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element.
1 Publication(Microbial infection) In complex with PREP1, binds to the 5'-TGATTGAC-3' consensus sequence in the U5 region of Moloney murine leukemia virus and promotes viral transcription.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 286 | Required for binding to the NFIL3 promoter1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 233 – 295 | Homeobox; TALE-typePROSITE-ProRule annotationAdd BLAST | 63 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- transcription corepressor binding Source: Ensembl
GO - Biological processi
- adrenal gland development Source: MGI
- animal organ morphogenesis Source: MGI
- anterior/posterior pattern specification Source: MGI
- brain development Source: GO_Central
- branching involved in ureteric bud morphogenesis Source: MGI
- embryonic hemopoiesis Source: MGI
- embryonic limb morphogenesis Source: MGI
- embryonic organ development Source: MGI
- embryonic skeletal system development Source: MGI
- eye development Source: GO_Central
- natural killer cell differentiation Source: UniProtKB
- negative regulation of DNA-binding transcription factor activity Source: MGI
- negative regulation of neuron differentiation Source: MGI
- neuron development Source: GO_Central
- positive regulation of cell population proliferation Source: MGI
- positive regulation of G2/M transition of mitotic cell cycle Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- proximal/distal pattern formation Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of ossification Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- sex differentiation Source: UniProtKB-KW
- spleen development Source: MGI
- steroid biosynthetic process Source: UniProtKB-KW
- thymus development Source: MGI
- urogenital system development Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Sexual differentiation, Steroidogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-B-cell leukemia transcription factor 1Alternative name(s): Homeobox protein PBX1 |
Gene namesi | Name:Pbx1 Synonyms:Pbx-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97495, Pbx1 |
VEuPathDBi | HostDB:ENSMUSG00000052534 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- cytoplasm Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 237 | R → A: Slightly reduced binding to NFIL3 promoter. 1 Publication | 1 | |
Mutagenesisi | 286 | N → A: Significantly reduced binding to NFIL3 promoter. 1 Publication | 1 | |
Mutagenesisi | 290 | R → A: Slightly reduced binding to NFIL3 promoter. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049236 | 1 – 430 | Pre-B-cell leukemia transcription factor 1Add BLAST | 430 |
Proteomic databases
MaxQBi | P41778 |
PaxDbi | P41778 |
PRIDEi | P41778 |
ProteomicsDBi | 287793 [P41778-1] 287794 [P41778-2] |
PTM databases
iPTMneti | P41778 |
PhosphoSitePlusi | P41778 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000052534, Expressed in rostral migratory stream and 311 other tissues |
Genevisiblei | P41778, MM |
Interactioni
Subunit structurei
Forms a heterodimer with MEIS1 which binds DNA (PubMed:9525891, PubMed:19799567). The PBX1-MEIS1 heterodimer binds a cAMP-responsive sequence in CYP17 (PubMed:9525891). It also binds a consensus region in the SOX3 promoter (PubMed:19799567). PBX1 forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10 (PubMed:9315626, PubMed:10082572, PubMed:10523646, PubMed:12409300). Forms heterodimers with HOXA1, HOXA5, HOXB7 and HOXB8 which bind the 5'-TGATTGAT-3' consensus sequence (By similarity). Also forms heterodimers with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4 which bind the 5'-ATCAATCAA-3' consensus sequence (PubMed:7791786).
Interacts with PBXIP1 (By similarity).
Interacts with TLX1 (By similarity).
Interacts with FOXC1 (By similarity).
Interacts with MN1 (By similarity).
By similarity7 PublicationsPart of a PDX1:PBX1b:MEIS2B complex; PBX1b recruits MEIS2B to the complex.
2 PublicationsBinary interactionsi
P41778
With | #Exp. | IntAct |
---|---|---|
Hoxa9 [P09631] | 2 | EBI-6996259,EBI-925334 |
Hoxb6 [P09023] | 2 | EBI-6996259,EBI-15992861 |
Hoxb8 [P09632] | 4 | EBI-6996259,EBI-925374 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: UniProtKB
- transcription corepressor binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 202037, 23 interactors |
CORUMi | P41778 |
DIPi | DIP-6107N |
IntActi | P41778, 20 interactors |
MINTi | P41778 |
STRINGi | 10090.ENSMUSP00000135516 |
Miscellaneous databases
RNActi | P41778, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P41778 |
SMRi | P41778 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P41778 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 232 | PBCPROSITE-ProRule annotationAdd BLAST | 195 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 45 – 124 | PBC-APROSITE-ProRule annotationAdd BLAST | 80 | |
Regioni | 127 – 232 | PBC-BPROSITE-ProRule annotationAdd BLAST | 106 | |
Regioni | 317 – 338 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 395 – 430 | DisorderedSequence analysisAdd BLAST | 36 |
Domaini
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0774, Eukaryota |
GeneTreei | ENSGT00940000154374 |
HOGENOMi | CLU_041153_1_0_1 |
InParanoidi | P41778 |
OMAi | NEYFYGH |
OrthoDBi | 804740at2759 |
PhylomeDBi | P41778 |
TreeFami | TF314340 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR005542, PBX |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03792, PBC, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS51978, PBC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI
60 70 80 90 100
MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP
110 120 130 140 150
TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY
160 170 180 190 200
RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM NLLREQSRTR PISPKEIERM
210 220 230 240 250
VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF NKQATEILNE
260 270 280 290 300
YFYSHLSNPY PSEEAKEELA KKCGITVSQV SNWFGNKRIR YKKNIGKFQE
310 320 330 340 350
EANIYAAKTA VTATNVSAHG SQANSPSTPN SAGSSSSFNM SNSGDLFMSV
360 370 380 390 400
QSLNGDSYQG AQVGANVQSQ VDTLRHVISQ TGGYSDGLAA SQMYSPQGIS
410 420 430
ANGGWQDATT PSSVTSPTEG PGSVHSDTSN
The sequence of this isoform differs from the canonical sequence as follows:
334-347: SSSSFNMSNSGDLF → GYPSPCYQPDRRIQ
348-430: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD9J2V6 | D9J2V6_MOUSE | Pre-B-cell leukemia transcription f... | Pbx1 PBX1 | 339 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002273 | 334 – 347 | SSSSF…SGDLF → GYPSPCYQPDRRIQ in isoform PBX1b. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_002274 | 348 – 430 | Missing in isoform PBX1b. 1 PublicationAdd BLAST | 83 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27453 mRNA Translation: AAA21832.1 AF020196 mRNA Translation: AAB71191.1 BC058390 mRNA Translation: AAH58390.1 |
CCDSi | CCDS15461.1 [P41778-1] CCDS15462.1 [P41778-2] |
PIRi | A54863 |
RefSeqi | NP_001278437.1, NM_001291508.1 [P41778-2] NP_032809.1, NM_008783.3 [P41778-2] NP_899198.1, NM_183355.3 [P41778-1] |
Genome annotation databases
Ensembli | ENSMUST00000072863; ENSMUSP00000072640; ENSMUSG00000052534 [P41778-2] ENSMUST00000176540; ENSMUSP00000135516; ENSMUSG00000052534 [P41778-1] ENSMUST00000176790; ENSMUSP00000134925; ENSMUSG00000052534 [P41778-2] ENSMUST00000188912; ENSMUSP00000140606; ENSMUSG00000052534 [P41778-2] |
GeneIDi | 18514 |
KEGGi | mmu:18514 |
UCSCi | uc007dle.2, mouse [P41778-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27453 mRNA Translation: AAA21832.1 AF020196 mRNA Translation: AAB71191.1 BC058390 mRNA Translation: AAH58390.1 |
CCDSi | CCDS15461.1 [P41778-1] CCDS15462.1 [P41778-2] |
PIRi | A54863 |
RefSeqi | NP_001278437.1, NM_001291508.1 [P41778-2] NP_032809.1, NM_008783.3 [P41778-2] NP_899198.1, NM_183355.3 [P41778-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DU6 | NMR | - | A | 241-294 | [»] | |
1LFU | NMR | - | P | 233-313 | [»] | |
BMRBi | P41778 | |||||
SMRi | P41778 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202037, 23 interactors |
CORUMi | P41778 |
DIPi | DIP-6107N |
IntActi | P41778, 20 interactors |
MINTi | P41778 |
STRINGi | 10090.ENSMUSP00000135516 |
PTM databases
iPTMneti | P41778 |
PhosphoSitePlusi | P41778 |
Proteomic databases
MaxQBi | P41778 |
PaxDbi | P41778 |
PRIDEi | P41778 |
ProteomicsDBi | 287793 [P41778-1] 287794 [P41778-2] |
Protocols and materials databases
Antibodypediai | 1079, 402 antibodies from 37 providers |
DNASUi | 18514 |
Genome annotation databases
Ensembli | ENSMUST00000072863; ENSMUSP00000072640; ENSMUSG00000052534 [P41778-2] ENSMUST00000176540; ENSMUSP00000135516; ENSMUSG00000052534 [P41778-1] ENSMUST00000176790; ENSMUSP00000134925; ENSMUSG00000052534 [P41778-2] ENSMUST00000188912; ENSMUSP00000140606; ENSMUSG00000052534 [P41778-2] |
GeneIDi | 18514 |
KEGGi | mmu:18514 |
UCSCi | uc007dle.2, mouse [P41778-1] |
Organism-specific databases
CTDi | 5087 |
MGIi | MGI:97495, Pbx1 |
VEuPathDBi | HostDB:ENSMUSG00000052534 |
Phylogenomic databases
eggNOGi | KOG0774, Eukaryota |
GeneTreei | ENSGT00940000154374 |
HOGENOMi | CLU_041153_1_0_1 |
InParanoidi | P41778 |
OMAi | NEYFYGH |
OrthoDBi | 804740at2759 |
PhylomeDBi | P41778 |
TreeFami | TF314340 |
Miscellaneous databases
BioGRID-ORCSi | 18514, 0 hits in 63 CRISPR screens |
ChiTaRSi | Pbx1, mouse |
EvolutionaryTracei | P41778 |
PROi | PR:P41778 |
RNActi | P41778, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052534, Expressed in rostral migratory stream and 311 other tissues |
Genevisiblei | P41778, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR005542, PBX |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03792, PBC, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS51978, PBC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PBX1_MOUSE | |
Accessioni | P41778Primary (citable) accession number: P41778 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | February 21, 2002 | |
Last modified: | February 23, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families