PRKCI

Functionⁱ

Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI₃K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation (By similarity).By similarity14 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.

Activity regulationⁱ

Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to the interaction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-412 (activation loop of the kinase domain) and Thr-564 (turn motif), need to be phosphorylated for its full activation (By similarity). Might also be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.By similarity1 Publication

Kineticsⁱ

K_M=
13.5 µM for ATP (for recombinant purified PRKCI)
1 Publication
Manual assertion based on experiment inⁱ
- Ref.10
  "Unique structural and functional properties of the ATP-binding domain of atypical protein kinase C-iota."
  Spitaler M., Villunger A., Grunicke H., Uberall F.
  J. Biol. Chem. 275:33289-33296(2000) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF LYS-274.

V_max=
7.4 pmol/min/mg enzyme
1 Publication
Manual assertion based on experiment inⁱ
- Ref.10
  "Unique structural and functional properties of the ATP-binding domain of atypical protein kinase C-iota."
  Spitaler M., Villunger A., Grunicke H., Uberall F.
  J. Biol. Chem. 275:33289-33296(2000) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF LYS-274.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	283	ATPPROSITE-ProRule annotation		1
Active siteⁱ	378	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	140 – 190	Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST		51
Nucleotide bindingⁱ	260 – 268	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Traceable author statementⁱ
- 8226978
metal ion binding Source: UniProtKB-KW
phospholipid binding
Source: UniProtKB
Inferred from direct assayⁱ
- 8226978
protein domain specific binding Source: Ensembl
protein kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11724794
protein kinase C activity Source: BHF-UCL
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 8226978

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin filament organization Source: Ensembl
bicellular tight junction assembly Source: Reactome
cell-cell junction organization
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11257119
cell migration Source: Ensembl
cellular response to insulin stimulus Source: BHF-UCL
cytoskeleton organization
Source: UniProtKB
Non-traceable author statementⁱ
- 11257119
establishment of apical/basal cell polarity Source: Ensembl
establishment or maintenance of epithelial cell apical/basal polarity
Source: UniProtKB
Traceable author statementⁱ
- 14676191
eye photoreceptor cell development Source: Ensembl
Golgi vesicle budding Source: Ensembl
intracellular signal transduction
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
membrane organization
Source: UniProtKB
Non-traceable author statementⁱ
- 11257119
negative regulation of apoptotic process Source: Reactome
negative regulation of glial cell apoptotic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21419810
negative regulation of neuron apoptotic process
Source: UniProtKB
Inferred from direct assayⁱ
- 10467349
peptidyl-serine phosphorylation
Source: WormBase
Inferred from direct assayⁱ
- 21983565
positive regulation of endothelial cell apoptotic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19327373
positive regulation of glial cell proliferation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21419810
positive regulation of glucose import Source: BHF-UCL
positive regulation of neuron projection development
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 10467349
positive regulation of NF-kappaB transcription factor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 10467349
positive regulation of Notch signaling pathway Source: Reactome
positive regulation of protein localization to plasma membrane Source: BHF-UCL
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 8226978
protein targeting to membrane
Source: UniProtKB
Non-traceable author statementⁱ
- 11257119
regulation of postsynaptic membrane neurotransmitter receptor levels Source: Ensembl
response to interleukin-1 Source: Ensembl
secretion
Source: UniProtKB
Non-traceable author statementⁱ
- 8226978
vesicle-mediated transport
Source: UniProtKB
Traceable author statementⁱ
- 11257119

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Ligand	ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAⁱ	2.7.11.13 2681
Reactomeⁱ	R-HSA-1912408 Pre-NOTCH Transcription and Translation R-HSA-209543 p75NTR recruits signalling complexes R-HSA-420029 Tight junction interactions
SABIO-RKⁱ	P41743
SignaLinkⁱ	P41743
SIGNORⁱ	P41743

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein kinase C iota type (EC:2.7.11.13) Alternative name(s): Atypical protein kinase C-lambda/iota Short name: PRKC-lambda/iota Short name: aPKC-lambda/iota nPKC-iota
Gene namesⁱ	Name:PRKCI Synonyms:DXS1179E
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 3

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000163558.12
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:9404 PRKCI
MIMⁱ	600539 gene
neXtProtⁱ	NX_P41743

Keywords - Cellular componentⁱ

Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	29	K → A: No effect on interaction with SQSTM1. 1 Publication	1
Mutagenesisⁱ	72	D → A: Loss of interaction with ECT2, PARD6A and with SQSTM1. 3 Publications	1
Mutagenesisⁱ	85	E → A: Slight decrease of interaction with PARD6A. Loss of interaction with PARD6A; when associated with A-91. 1 Publication	1
Mutagenesisⁱ	91	R → A: Slight decrease of interaction with PARD6A. Loss of interaction with PARD6A; when associated with A-85. 1 Publication	1
Mutagenesisⁱ	265	Y → F: No effect on the SRC-mediated phosphorylation state. No effect on SRC-induced enzyme activity. Little effect on TRAF6-mediated activation of NF-kappa-B. Decreased binding to KPNB1/importin-beta. 2 Publications	1
Mutagenesisⁱ	274	K → R: No effect on activity. 1 Publication	1
Mutagenesisⁱ	274	K → W: Abolishes activity. 1 Publication	1
Mutagenesisⁱ	280	Y → F: No effect on the SRC-mediated phosphorylation state. No effect on SRC-induced enzyme activity. No effect on TRAF6-mediated activation of NF-kappa-B. 1 Publication	1
Mutagenesisⁱ	334	Y → F: No effect on the SRC-mediated phosphorylation state. Significant reduction of SRC-induced enzyme activity. Greatly reduced TRAF6-mediated activation of NF-kappa-B. Reduces NGF-dependent cell survival. 1 Publication	1

Keywords - Diseaseⁱ

Proto-oncogene, Tumor suppressor

Organism-specific databases

DisGeNET More...DisGeNETⁱ	5584
Open Targets More...OpenTargetsⁱ	ENSG00000163558
PharmGKBⁱ	PA33768

Chemistry databases

ChEMBLⁱ	CHEMBL2598
Drug and drug target database More...DrugBankⁱ	DB03777 Rbt205 Inhibitor DB00675 Tamoxifen
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	1490

Polymorphism and mutation databases

BioMutaⁱ	PRKCI
DMDMⁱ	239938658

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000055710	2 – 596	Protein kinase C iota typeAdd BLAST		595

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylprolineCombined sources	1
Modified residueⁱ	3	PhosphothreonineCombined sources	1
Modified residueⁱ	7	PhosphoserineCombined sources	1
Modified residueⁱ	8	PhosphoserineCombined sources	1
Modified residueⁱ	9	PhosphothreonineCombined sources	1
Modified residueⁱ	265	Phosphotyrosine; by SRC2 Publications	1
Modified residueⁱ	280	Phosphotyrosine; by SRC1 Publication	1
Modified residueⁱ	334	Phosphotyrosine; by SRC1 Publication	1
Modified residueⁱ	412	Phosphothreonine; by PDPK11 Publication	1
Modified residueⁱ	564	PhosphothreonineCombined sources1 Publication	1

Post-translational modificationⁱ

Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity). Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334 (PubMed:11713277, PubMed:16452474). Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus (PubMed:11891849). Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation (PubMed:11713277). Phosphorylated at Thr-564 during the initial phase of long term potentiation (By similarity).By similarity3 Publications

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	P41743
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P41743
PaxDbⁱ	P41743
PeptideAtlas More...PeptideAtlasⁱ	P41743
PRIDEⁱ	P41743
ProteomicsDBⁱ	55477

PTM databases

iPTMnetⁱ	P41743
PhosphoSitePlusⁱ	P41743

Expressionⁱ

Tissue specificityⁱ

Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.3 Publications

Gene expression databases

Bgeeⁱ	ENSG00000163558 Expressed in 236 organ(s), highest expression level in testis
CleanExⁱ	HS_PRKCI
Genevisibleⁱ	P41743 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA026574 HPA038635

HPAⁱ

HPA026574
HPA038635

Interactionⁱ

Subunit structureⁱ

Forms a complex with SQSTM1 and MP2K5 (By similarity). Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts with PARD6A, PARD6B and PARD6G. Part of a quaternary complex containing aPKC, PARD3, a PARD6 protein (PARD6A, PARD6B or PARD6G) and a GTPase protein (CDC42 or RAC1). Part of a complex with LLGL1 and PARD6B. Interacts with ADAP1/CENTA1. Interaction with SMG1, through the ZN-finger domain, activates the kinase activity. Interacts with CDK7. Forms a complex with RAB2A and GAPDH involved in recruitment onto the membrane of vesicular tubular clusters (VTCs). Interacts with ECT2 ('Thr-359' phosphorylated form). Interacts with VAMP2 (PubMed:17313651). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529).By similarityCurated17 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CDC42	P60953	5	EBI-286199,EBI-81752
ELF3	P78545	2	EBI-286199,EBI-1057285
GLI1	P08151-1	3	EBI-286199,EBI-16038799
MARK4	Q96L34	2	EBI-286199,EBI-302319
PARD3	Q8TEW0	3	EBI-286199,EBI-81968
PARD6A	Q9NPB6	12	EBI-286199,EBI-81876
PARD6A	Q9NPB6-2	4	EBI-286199,EBI-10693102
PARD6B	Q9BYG5	15	EBI-286199,EBI-295391
PARD6G	Q9BYG4	5	EBI-286199,EBI-295417
PNMA1	Q8ND90	2	EBI-286199,EBI-302345
PRKCZ	Q05513	4	EBI-286199,EBI-295351
RAC1	P63000	3	EBI-286199,EBI-413628
SOX2	P48431	2	EBI-286199,EBI-6124081
SQSTM1	Q13501	7	EBI-286199,EBI-307104
YWHAH	Q04917	3	EBI-286199,EBI-306940

GO - Molecular functionⁱ

protein domain specific binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	111570, 93 interactors
CORUMⁱ	P41743
Database of interacting proteins More...DIPⁱ	DIP-31311N
ELMⁱ	P41743
IntActⁱ	P41743, 56 interactors
Molecular INTeraction database More...MINTⁱ	P41743
STRINGⁱ	9606.ENSP00000295797

Chemistry databases

BindingDBⁱ

P41743

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	26 – 32	Combined sources	7
Beta strandⁱ	35 – 41	Combined sources	7
Helixⁱ	47 – 57	Combined sources	11
Beta strandⁱ	67 – 71	Combined sources	5
Beta strandⁱ	73 – 75	Combined sources	3
Beta strandⁱ	77 – 79	Combined sources	3
Helixⁱ	83 – 95	Combined sources	13
Beta strandⁱ	101 – 106	Combined sources	6
Helixⁱ	251 – 253	Combined sources	3
Beta strandⁱ	254 – 262	Combined sources	9
Beta strandⁱ	264 – 273	Combined sources	10
Turnⁱ	274 – 277	Combined sources	4
Beta strandⁱ	279 – 286	Combined sources	8
Helixⁱ	287 – 289	Combined sources	3
Helixⁱ	293 – 309	Combined sources	17
Beta strandⁱ	318 – 323	Combined sources	6
Beta strandⁱ	325 – 332	Combined sources	8
Helixⁱ	340 – 347	Combined sources	8
Helixⁱ	352 – 371	Combined sources	20
Helixⁱ	381 – 383	Combined sources	3
Beta strandⁱ	384 – 386	Combined sources	3
Beta strandⁱ	388 – 390	Combined sources	3
Beta strandⁱ	392 – 394	Combined sources	3
Helixⁱ	397 – 399	Combined sources	3
Helixⁱ	417 – 419	Combined sources	3
Helixⁱ	422 – 425	Combined sources	4
Helixⁱ	433 – 448	Combined sources	16
Turnⁱ	452 – 457	Combined sources	6
Helixⁱ	467 – 476	Combined sources	10
Helixⁱ	487 – 496	Combined sources	10
Turnⁱ	501 – 503	Combined sources	3
Turnⁱ	505 – 507	Combined sources	3
Turnⁱ	509 – 511	Combined sources	3
Helixⁱ	512 – 517	Combined sources	6
Helixⁱ	520 – 522	Combined sources	3
Helixⁱ	527 – 531	Combined sources	5
Turnⁱ	545 – 547	Combined sources	3
Helixⁱ	549 – 551	Combined sources	3
Helixⁱ	554 – 557	Combined sources	4
Helixⁱ	568 – 571	Combined sources	4
Helixⁱ	576 – 579	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P41743
SMRⁱ	P41743
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P41743

EvolutionaryTraceⁱ

P41743

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	25 – 108	PB1PROSITE-ProRule annotationAdd BLAST	84
Domainⁱ	254 – 522	Protein kinasePROSITE-ProRule annotationAdd BLAST	269
Domainⁱ	523 – 594	AGC-kinase C-terminalAdd BLAST	72

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	2 – 253	Regulatory domainAdd BLAST	252
Regionⁱ	2 – 28	Required for interaction with RAB2Add BLAST	27
Regionⁱ	72 – 91	Interaction with PARD6AAdd BLAST	20

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	125 – 134	PseudosubstrateBy similarity		10

Domainⁱ

The PB1 domain mediates interaction with SQSTM1.By similarity

The C1 zinc finger does not bind diacylglycerol (DAG).

The pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins, except the presence of a non-phosphorylatable residue in place of Ser, it modulates activity by competing with substrates.By similarity

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	140 – 190	Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST		51

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0695 Eukaryota ENOG410ZMG2 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150815
HOGENOMⁱ	HOG000233033
HOVERGENⁱ	HBG108317
InParanoidⁱ	P41743
KEGG Orthology (KO) More...KOⁱ	K06069
OMAⁱ	MMPMDQS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03Q9
PhylomeDBⁱ	P41743
TreeFamⁱ	TF102004

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00029 C1, 1 hit cd06404 PB1_aPKC, 1 hit cd05618 STKc_aPKC_iota, 1 hit
InterProⁱ	View protein in InterPro IPR000961 AGC-kinase_C IPR034661 aPKC_iota IPR020454 DAG/PE-bd IPR011009 Kinase-like_dom_sf IPR034877 PB1_aPKC IPR000270 PB1_dom IPR002219 PE/DAG-bd IPR012233 PKC IPR017892 Pkinase_C IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00130 C1_1, 1 hit PF00564 PB1, 1 hit PF00069 Pkinase, 1 hit PF00433 Pkinase_C, 1 hit
PIRSFⁱ	PIRSF000554 PKC_zeta, 1 hit
PRINTSⁱ	PR00008 DAGPEDOMAIN
SMARTⁱ	View protein in SMART SM00109 C1, 1 hit SM00666 PB1, 1 hit SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS51745 PB1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS00479 ZF_DAG_PE_1, 1 hit PS50081 ZF_DAG_PE_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P41743-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPTQRDSSTM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL 
        60         70         80         90        100
CNEVRDMCSF DNEQLFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE 
       110        120        130        140        150
LLIHVFPCVP ERPGMPCPGE DKSIYRRGAR RWRKLYCANG HTFQAKRFNR 
       160        170        180        190        200
RAHCAICTDR IWGLGRQGYK CINCKLLVHK KCHKLVTIEC GRHSLPQEPV 
       210        220        230        240        250
MPMDQSSMHS DHAQTVIPYN PSSHESLDQV GEEKEAMNTR ESGKASSSLG 
       260        270        280        290        300
LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ 
       310        320        330        340        350
TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL 
       360        370        380        390        400
PEEHARFYSA EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC 
       410        420        430        440        450
KEGLRPGDTT STFCGTPNYI APEILRGEDY GFSVDWWALG VLMFEMMAGR 
       460        470        480        490        500
SPFDIVGSSD NPDQNTEDYL FQVILEKQIR IPRSLSVKAA SVLKSFLNKD 
       510        520        530        540        550
PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK PNISGEFGLD 
       560        570        580        590 
NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV

Length:596

Mass (Da):68,262

Last modified:June 16, 2009 - v2

Checksum:ⁱ1E3F8C1D4BFC734F

GO

Sequence cautionⁱ

The sequence AAA60171 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAB17011 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAH22016 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	485	L → M in AAH22016 (PubMed:15489334).Curated	1
Sequence conflictⁱ	508	H → L in AAH22016 (PubMed:15489334).Curated	1
Sequence conflictⁱ	560	P → R in AAH22016 (PubMed:15489334).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_042322	118	P → L in a metastatic melanoma sample; somatic mutation. 1 Publication		1
Natural variantⁱVAR_042323	130	R → C1 PublicationCorresponds to variant dbSNP:rs56154494Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L18964 mRNA Translation: AAA60171.1 Different initiation. L33881 mRNA Translation: AAB17011.1 Different initiation. CH471052 Genomic DNA Translation: EAW78513.1 CH471052 Genomic DNA Translation: EAW78515.1 BC022016 mRNA Translation: AAH22016.3 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS3212.2
PIRⁱ	A49509
NCBI Reference Sequences More...RefSeqⁱ	NP_002731.4, NM_002740.5
UniGeneⁱ	Hs.478199

Genome annotation databases

Ensemblⁱ	ENST00000295797; ENSP00000295797; ENSG00000163558
GeneIDⁱ	5584
KEGGⁱ	hsa:5584
UCSC genome browser More...UCSCⁱ	uc003fgs.3 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P41743	Protein kinase C		PANTR		596	UniRef100_P41743
Protein kinase C		PANPA		596
UPI00020E325E		PANTR		690

Protein	Similar proteins		Species	Score	Length	Source
P41743	Protein kinase C iota type	)	MOUSE		595	UniRef90_P41743
Protein kinase C		PANTR		596
Protein kinase C		PANPA		596
Protein kinase C		DELLE		707
Protein kinase C		PHYCD		482
+70

Protein	Similar proteins		Species	Score	Length	Source
P41743	Protein kinase C iota type	)	MOUSE		595	UniRef50_P41743
Protein kinase C		PANTR		596
Protein kinase C		PANPA		596
Protein kinase C		DELLE		707
Protein kinase C		PHYCD		482
+105

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L18964 mRNA Translation: AAA60171.1 Different initiation. L33881 mRNA Translation: AAB17011.1 Different initiation. CH471052 Genomic DNA Translation: EAW78513.1 CH471052 Genomic DNA Translation: EAW78515.1 BC022016 mRNA Translation: AAH22016.3 Different initiation.
CCDSⁱ	CCDS3212.2
PIRⁱ	A49509
RefSeqⁱ	NP_002731.4, NM_002740.5
UniGeneⁱ	Hs.478199

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1VD2	NMR	-	A	25-108	[»]
	1WMH	X-ray	1.50	A	25-108	[»]
	1ZRZ	X-ray	3.00	A	233-596	[»]
	3A8W	X-ray	2.10	A/B	249-588	[»]
	3A8X	X-ray	2.00	A/B	249-588	[»]
	3ZH8	X-ray	2.74	A/B/C	248-594	[»]
	5LI1	X-ray	2.00	A	248-596	[»]
	5LI9	X-ray	1.79	A	248-596	[»]
	5LIH	X-ray	3.25	A/B	248-596	[»]
ProteinModelPortalⁱ	P41743
SMRⁱ	P41743
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	111570, 93 interactors
CORUMⁱ	P41743
DIPⁱ	DIP-31311N
ELMⁱ	P41743
IntActⁱ	P41743, 56 interactors
MINTⁱ	P41743
STRINGⁱ	9606.ENSP00000295797

Chemistry databases

BindingDBⁱ	P41743
ChEMBLⁱ	CHEMBL2598
DrugBankⁱ	DB03777 Rbt205 Inhibitor DB00675 Tamoxifen
GuidetoPHARMACOLOGYⁱ	1490

PTM databases

iPTMnetⁱ	P41743
PhosphoSitePlusⁱ	P41743

Polymorphism and mutation databases

BioMutaⁱ	PRKCI
DMDMⁱ	239938658

Proteomic databases

EPDⁱ	P41743
MaxQBⁱ	P41743
PaxDbⁱ	P41743
PeptideAtlasⁱ	P41743
PRIDEⁱ	P41743
ProteomicsDBⁱ	55477

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	5584
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000295797; ENSP00000295797; ENSG00000163558
GeneIDⁱ	5584
KEGGⁱ	hsa:5584
UCSCⁱ	uc003fgs.3 human

Organism-specific databases

CTDⁱ	5584
DisGeNETⁱ	5584
EuPathDBⁱ	HostDB:ENSG00000163558.12
GeneCardsⁱ	PRKCI
HGNCⁱ	HGNC:9404 PRKCI
HPAⁱ	HPA026574 HPA038635
MIMⁱ	600539 gene
neXtProtⁱ	NX_P41743
OpenTargetsⁱ	ENSG00000163558
PharmGKBⁱ	PA33768
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0695 Eukaryota ENOG410ZMG2 LUCA
GeneTreeⁱ	ENSGT00930000150815
HOGENOMⁱ	HOG000233033
HOVERGENⁱ	HBG108317
InParanoidⁱ	P41743
KOⁱ	K06069
OMAⁱ	MMPMDQS
OrthoDBⁱ	EOG091G03Q9
PhylomeDBⁱ	P41743
TreeFamⁱ	TF102004

Enzyme and pathway databases

BRENDAⁱ	2.7.11.13 2681
Reactomeⁱ	R-HSA-1912408 Pre-NOTCH Transcription and Translation R-HSA-209543 p75NTR recruits signalling complexes R-HSA-420029 Tight junction interactions
SABIO-RKⁱ	P41743
SignaLinkⁱ	P41743
SIGNORⁱ	P41743

Miscellaneous databases

ChiTaRSⁱ	PRKCI human
EvolutionaryTraceⁱ	P41743
GeneWikiⁱ	PRKCI
GenomeRNAiⁱ	5584
Protein Ontology More...PROⁱ	PR:P41743
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000163558 Expressed in 236 organ(s), highest expression level in testis
CleanExⁱ	HS_PRKCI
Genevisibleⁱ	P41743 HS

Family and domain databases

CDDⁱ	cd00029 C1, 1 hit cd06404 PB1_aPKC, 1 hit cd05618 STKc_aPKC_iota, 1 hit
InterProⁱ	View protein in InterPro IPR000961 AGC-kinase_C IPR034661 aPKC_iota IPR020454 DAG/PE-bd IPR011009 Kinase-like_dom_sf IPR034877 PB1_aPKC IPR000270 PB1_dom IPR002219 PE/DAG-bd IPR012233 PKC IPR017892 Pkinase_C IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfamⁱ	View protein in Pfam PF00130 C1_1, 1 hit PF00564 PB1, 1 hit PF00069 Pkinase, 1 hit PF00433 Pkinase_C, 1 hit
PIRSFⁱ	PIRSF000554 PKC_zeta, 1 hit
PRINTSⁱ	PR00008 DAGPEDOMAIN
SMARTⁱ	View protein in SMART SM00109 C1, 1 hit SM00666 PB1, 1 hit SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS51745 PB1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS00479 ZF_DAG_PE_1, 1 hit PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KPCI_HUMAN
Accessionⁱ	P41743Primary (citable) accession number: P41743 Secondary accession number(s): D3DNQ4, Q8WW06
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	June 16, 2009
	Last modified:	November 7, 2018
	This is version 209 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

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UniProtKB - P41743 (KPCI_HUMAN)

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Zinc finger

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<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

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Keywords - Coding sequence diversityi

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Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

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GO - Molecular functionⁱ

Structureⁱ

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Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ