UniProtKB - P41739 (ARNT_RAT)
Aryl hydrocarbon receptor nuclear translocator
Arnt
Functioni
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (By similarity).
By similarityGO - Molecular functioni
- aryl hydrocarbon receptor binding Source: RGD
- cis-regulatory region sequence-specific DNA binding Source: RGD
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein-containing complex binding Source: RGD
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: UniProtKB
GO - Biological processi
- cell differentiation Source: RGD
- embryonic placenta development Source: RGD
- intracellular receptor signaling pathway Source: GOC
- positive regulation of hormone biosynthetic process Source: RGD
- positive regulation of protein sumoylation Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of vascular endothelial growth factor production Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: RGD
- response to dexamethasone Source: RGD
- response to hypoxia Source: RGD
- response to toxic substance Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-RNO-1234158, Regulation of gene expression by Hypoxia-inducible Factor R-RNO-211945, Phase I - Functionalization of compounds R-RNO-211976, Endogenous sterols R-RNO-211981, Xenobiotics R-RNO-8937144, Aryl hydrocarbon receptor signalling |
Names & Taxonomyi
Protein namesi | Recommended name: Aryl hydrocarbon receptor nuclear translocatorShort name: ARNT protein Alternative name(s): Dioxin receptor, nuclear translocator Hypoxia-inducible factor 1-beta Short name: HIF-1-beta Short name: HIF1-beta |
Gene namesi | Name:Arnt |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2153, Arnt |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: RGD
- RNA polymerase II transcription regulator complex Source: RGD
Other locations
- aryl hydrocarbon receptor complex Source: GO_Central
- cytoplasm Source: RGD
- transcription regulator complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000127121 | 2 – 800 | Aryl hydrocarbon receptor nuclear translocatorAdd BLAST | 799 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Cross-linki | 58 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 77 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P41739 |
PTM databases
iPTMneti | P41739 |
PhosphoSitePlusi | P41739 |
Interactioni
Subunit structurei
Monomer. Homodimer only upon binding to a DNA (By similarity). Efficient DNA binding requires dimerization with another bHLH protein.
Interacts with TACC3 (By similarity).
Interacts with HIF1A, EPAS1, NPAS1 and NPAS3; forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity).
Forms a heterodimer with AHRR, as well as with other bHLH proteins.
Interacts with NOCA7 (By similarity).
Interacts with TACC3 (By similarity).
Interacts with AHR; the heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (By similarity).
Interacts with SIM1 and NPAS4 (By similarity).
By similarityBinary interactionsi
P41739
With | #Exp. | IntAct |
---|---|---|
Ahr [P41738] | 2 | EBI-1162920,EBI-1162880 |
GO - Molecular functioni
- aryl hydrocarbon receptor binding Source: RGD
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247282, 1 interactor |
IntActi | P41739, 1 interactor |
STRINGi | 10116.ENSRNOP00000060764 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 89 – 142 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 161 – 235 | PAS 1PROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 349 – 419 | PAS 2PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 424 – 467 | PACAdd BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 33 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 73 – 95 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 88 – 128 | DNA-bindingBy similarityAdd BLAST | 41 | |
Regioni | 112 – 264 | Required for heterodimer formation with EPAS1By similarityAdd BLAST | 153 | |
Regioni | 112 – 168 | Required for heterodimer formation with HIF1ABy similarityAdd BLAST | 57 | |
Regioni | 167 – 171 | Mediates the transcription activity and dimerization of the AHR:ARNT complexBy similarity | 5 | |
Regioni | 465 – 487 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 499 – 520 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 536 – 566 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 644 – 676 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 690 – 726 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 739 – 800 | DisorderedSequence analysisAdd BLAST | 62 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 19 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 551 – 565 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 742 – 789 | Polar residuesSequence analysisAdd BLAST | 48 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3561, Eukaryota |
InParanoidi | P41739 |
OrthoDBi | 331262at2759 |
PhylomeDBi | P41739 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR001067, Nuc_translocat IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit |
PRINTSi | PR00785, NCTRNSLOCATR |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00086, PAC, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAATTANPEM TSDVPSLGPT IASGNPGPGI QGGGAVVQRA IKRRSGLDFD
60 70 80 90 100
DEGEVNSKFL RCDDEQMCND KERFARSDDE QSSADKERLA RENHSEIERR
110 120 130 140 150
RRNKMTAYIT ELSDMVPTCS ALARKPDKLT ILRMAVSHMK SLRGTGNTST
160 170 180 190 200
DGSYKPSFLT DQELKHLILE AADGFLFIVS CETGRVVYVS DSVTPVLNQP
210 220 230 240 250
QSEWFGSTLY DQVHPDDVDK LREQLSTSEN ALTGRILDLK TGTVKKEGQQ
260 270 280 290 300
SSMRMCMGSR RSFICRMRCG TSSVDPVSMN RLSFLRNRCR NGLGSVKEGE
310 320 330 340 350
PHFVVVHCTG YIKAWPPAGV SLPDDDPEAG QGSKFCLVAI GRLQVTSSPN
360 370 380 390 400
CTDMSNICQP TEFISRHNIE GIFTFVDHRC VATVGYQPQE LLGKNIVEFC
410 420 430 440 450
HPEDQQLLRD SFQQVVKLKG QVLSVMFRFR AKNREWLWMR TSSFTFQNPY
460 470 480 490 500
SDEMSIFICT NTNVKNSSQE PRPTLSNTIQ RSQLGPTTNL SLEMGTGVLA
510 520 530 540 550
SRQQQQQQQQ QQQQQQQQTE LDMVPGRDGL ASYSHSQVSV QPVATAGSEH
560 570 580 590 600
SKPLEKSEGL FVQDRDPRFS EIYPNISADQ SKGLSSSTVP ATQQLFSQGS
610 620 630 640 650
SFPPNPRPAE NFRNSGLTPP VTIVQPSSSA GQILAQISRH SNLTQGSAPT
660 670 680 690 700
WTSSTRPGFS AQLPTQATAK TRSSQFGVNN FQTSSSFSAM SLPGAPTASP
710 720 730 740 750
STAAYPTLPN RGSNFPPETG QTTGQFQTRT AEGVGVWPQW QGQQPHHRSS
760 770 780 790 800
SNEQHVQPTS AQPSSQPEVF QEMLSMLGDQ SNTYNNEEFP DLTMFPPFSE
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LMF9 | F1LMF9_RAT | Aryl hydrocarbon receptor nuclear t... | Arnt | 810 | Annotation score: | ||
E9PTS2 | E9PTS2_RAT | Aryl hydrocarbon receptor nuclear t... | Arnt | 777 | Annotation score: | ||
A0A0G2K804 | A0A0G2K804_RAT | Aryl hydrocarbon receptor nuclear t... | Arnt | 795 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U61184 mRNA Translation: AAB03811.1 U08986 mRNA Translation: AAA56896.1 |
PIRi | S58376 |
RefSeqi | NP_036912.1, NM_012780.1 |
Genome annotation databases
GeneIDi | 25242 |
KEGGi | rno:25242 |
UCSCi | RGD:2153, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U61184 mRNA Translation: AAB03811.1 U08986 mRNA Translation: AAA56896.1 |
PIRi | S58376 |
RefSeqi | NP_036912.1, NM_012780.1 |
3D structure databases
AlphaFoldDBi | P41739 |
SMRi | P41739 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247282, 1 interactor |
IntActi | P41739, 1 interactor |
STRINGi | 10116.ENSRNOP00000060764 |
PTM databases
iPTMneti | P41739 |
PhosphoSitePlusi | P41739 |
Proteomic databases
PaxDbi | P41739 |
Protocols and materials databases
DNASUi | 25242 |
Genome annotation databases
GeneIDi | 25242 |
KEGGi | rno:25242 |
UCSCi | RGD:2153, rat |
Organism-specific databases
CTDi | 405 |
RGDi | 2153, Arnt |
Phylogenomic databases
eggNOGi | KOG3561, Eukaryota |
InParanoidi | P41739 |
OrthoDBi | 331262at2759 |
PhylomeDBi | P41739 |
Enzyme and pathway databases
Reactomei | R-RNO-1234158, Regulation of gene expression by Hypoxia-inducible Factor R-RNO-211945, Phase I - Functionalization of compounds R-RNO-211976, Endogenous sterols R-RNO-211981, Xenobiotics R-RNO-8937144, Aryl hydrocarbon receptor signalling |
Miscellaneous databases
PROi | PR:P41739 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR001067, Nuc_translocat IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit |
PRINTSi | PR00785, NCTRNSLOCATR |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00086, PAC, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ARNT_RAT | |
Accessioni | P41739Primary (citable) accession number: P41739 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |