UniProtKB - P41738 (AHR_RAT)
Aryl hydrocarbon receptor
Ahr
Functioni
Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed:7812217).
Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation. Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists. Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands. Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (By similarity).
By similarity1 PublicationGO - Molecular functioni
- aryl hydrocarbon receptor binding Source: RGD
- chaperone binding Source: RGD
- cis-regulatory region sequence-specific DNA binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- E-box binding Source: UniProtKB
- Hsp90 protein binding Source: RGD
- identical protein binding Source: RGD
- nuclear receptor activity Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: UniProtKB
- TBP-class protein binding Source: RGD
- TFIID-class transcription factor complex binding Source: RGD
- toxic substance binding Source: RGD
- transcription cis-regulatory region binding Source: RGD
- transcription coactivator binding Source: RGD
GO - Biological processi
- apoptotic process Source: UniProtKB
- B cell homeostasis Source: RGD
- blood circulation Source: RGD
- blood vessel development Source: RGD
- blood vessel diameter maintenance Source: RGD
- blood vessel morphogenesis Source: RGD
- blood vessel remodeling Source: RGD
- branching involved in blood vessel morphogenesis Source: RGD
- camera-type eye development Source: RGD
- cAMP-mediated signaling Source: RGD
- cardiac left ventricle morphogenesis Source: RGD
- cell cycle Source: UniProtKB-KW
- cell morphogenesis Source: RGD
- cellular response to 2,3,7,8-tetrachlorodibenzodioxine Source: UniProtKB
- cellular response to 3-methylcholanthrene Source: RGD
- cellular response to cAMP Source: RGD
- cellular response to forskolin Source: RGD
- cellular response to glucocorticoid stimulus Source: RGD
- circadian regulation of gene expression Source: UniProtKB
- circumferential growth involved in left ventricle morphogenesis Source: RGD
- common bile duct development Source: RGD
- embryonic hemopoiesis Source: RGD
- gland development Source: RGD
- glomerulus morphogenesis Source: RGD
- immune system process Source: RGD
- kidney morphogenesis Source: RGD
- liver development Source: RGD
- lymphocyte homeostasis Source: RGD
- negative regulation of alkaline phosphatase activity Source: RGD
- negative regulation of calcium ion transmembrane transport Source: RGD
- negative regulation of DNA biosynthetic process Source: RGD
- negative regulation of necrotic cell death Source: RGD
- negative regulation of osteoblast differentiation Source: RGD
- negative regulation of osteoblast proliferation Source: RGD
- negative regulation of systemic arterial blood pressure Source: RGD
- negative regulation of T cell mediated immune response to tumor cell Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of vasoconstriction Source: RGD
- ovarian follicle development Source: RGD
- positive regulation of cell size Source: RGD
- positive regulation of growth rate Source: RGD
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- post-embryonic hemopoiesis Source: RGD
- prostate gland development Source: RGD
- regulation of adaptive immune response Source: UniProtKB
- regulation of B cell proliferation Source: RGD
- regulation of cell cycle Source: UniProtKB
- regulation of gene expression Source: RGD
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: RGD
- reproductive structure development Source: RGD
- response to estradiol Source: RGD
- response to organic cyclic compound Source: RGD
- response to toxic substance Source: RGD
- response to xenobiotic stimulus Source: UniProtKB
- smooth muscle tissue development Source: RGD
- spleen development Source: RGD
- T cell homeostasis Source: RGD
- transcription by RNA polymerase II Source: UniProtKB
- venous blood vessel development Source: RGD
- xenobiotic metabolic process Source: InterPro
Keywordsi
Molecular function | Activator, DNA-binding, Receptor, Repressor |
Biological process | Biological rhythms, Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-RNO-211945, Phase I - Functionalization of compounds R-RNO-211976, Endogenous sterols R-RNO-211981, Xenobiotics R-RNO-8937144, Aryl hydrocarbon receptor signalling |
Names & Taxonomyi
Protein namesi | Recommended name: Aryl hydrocarbon receptorShort name: Ah receptor Short name: AhR |
Gene namesi | Name:Ahr |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2074, Ahr |
Subcellular locationi
Cytosol
- cytosol Source: RGD
Nucleus
- nucleus Source: UniProtKB
Other locations
- aryl hydrocarbon receptor complex Source: GO_Central
- cytoplasm Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000013464 | 1 – 9 | By similarity | 9 | |
ChainiPRO_0000013465 | 10 – 853 | Aryl hydrocarbon receptorAdd BLAST | 844 |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylationProteomic databases
PaxDbi | P41738 |
PRIDEi | P41738 |
PTM databases
iPTMneti | P41738 |
PhosphoSitePlusi | P41738 |
Expressioni
Tissue specificityi
Inductioni
Interactioni
Subunit structurei
Homodimer (By similarity). Heterodimer; efficient DNA binding requires dimerization with another bHLH protein.
Interacts with ARNT; the heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (By similarity). Binds MYBBP1A (By similarity).
Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT.
Interacts with NEDD8 and IVNS1ABP (By similarity).
Interacts with ARNTL/BMAL1.
Interacts with HSP90AB1 (By similarity).
Interacts with TIPARP; leading to mono-ADP-ribosylation of AHR and subsequent inhibition of AHR (By similarity).
By similarityBinary interactionsi
P41738
With | #Exp. | IntAct |
---|---|---|
Arnt [P41739] | 2 | EBI-1162880,EBI-1162920 |
GO - Molecular functioni
- aryl hydrocarbon receptor binding Source: RGD
- chaperone binding Source: RGD
- Hsp90 protein binding Source: RGD
- identical protein binding Source: RGD
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- TBP-class protein binding Source: RGD
- TFIID-class transcription factor complex binding Source: RGD
- transcription coactivator binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247719, 4 interactors |
IntActi | P41738, 2 interactors |
STRINGi | 10116.ENSRNOP00000006618 |
Chemistry databases
BindingDBi | P41738 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 79 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 116 – 179 | PAS 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 273 – 340 | PAS 2PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 346 – 384 | PACAdd BLAST | 39 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 38 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 37 – 65 | DNA-bindingBy similarityAdd BLAST | 29 | |
Regioni | 116 – 124 | Required for maintaining the overall integrity of the AHR:ARNT heterodimer and its transcriptional activityBy similarity | 9 | |
Regioni | 264 – 266 | Required for maintaining the overall integrity of the AHR:ARNT heterodimer and its transcriptional activityBy similarity | 3 | |
Regioni | 429 – 451 | DisorderedSequence analysisAdd BLAST | 23 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3560, Eukaryota |
InParanoidi | P41738 |
PhylomeDBi | P41738 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR033348, AHR IPR039091, AHR/AHRR IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 |
PANTHERi | PTHR10649, PTHR10649, 1 hit PTHR10649:SF9, PTHR10649:SF9, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit PF08447, PAS_3, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00086, PAC, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSGANITYA SRKRRKPVQK TVKPVPAEGI KSNPSKRHRD RLNTELDRLA
60 70 80 90 100
SLLPFPQDVI NKLDKLSVLR LSVSYLRAKS FFDVALKSTP ADRSRGQDQC
110 120 130 140 150
RAQVRDWQDL QEGEFLLQAL NGFVLVVTAD ALVFYASSTI QDYLGFQQSD
160 170 180 190 200
VIHQSVYELI HTEDRAEFQR QLHWALNPSQ CTDSAQGVDE THGLPQPAVY
210 220 230 240 250
YTPDQLPPEN TAFMERCFRC RLRCLLDNSS GFLAMNFQGR LKYLHGQNKK
260 270 280 290 300
GKDGALLPPQ LALFAIATPL QPPSILEIRT KNFIFRTKHK LDFTPIGCDA
310 320 330 340 350
KGQLILGYTE VELCNKGSGY QFIHAADMLH CAESHIRMIK TGESGMTVFR
360 370 380 390 400
LLAKHSRWRW VQSNARLIYR NGRPDYIIAT QRPLTDEEGR EHLQKRSMTL
410 420 430 440 450
PFMFATGEAV LYEISSPFSP IMDPLPIRTK SNTSRKDWAP QSTPSKDSFH
460 470 480 490 500
PNSLMSALIQ QDESIYLCPP SSPAPLDSHF LMDSMSECGS WQGSFAVASN
510 520 530 540 550
EALLKHEEIR HTQDVNLTLS GGPSELFPDN KNNDLYSIMR NLGIDFEDIR
560 570 580 590 600
SMQNEEFFRT DSSGEVDFKD IDITDEILTY VQDSLNNSTL LNSACQQQPV
610 620 630 640 650
SQHLSCMLQE RLQLEQQQQL QQQHPTQTLE PQRQLCQVEV PQHELGQKTK
660 670 680 690 700
HMQVNGMFAS WNPAPPVSFS CPQQERKHYS LFSGLQGTAQ EFPYKSEVDS
710 720 730 740 750
MPYTQNFAPC NQSLLPEHSK GTQLDFPGRD FERSLHPNAS NLEDFVSCLQ
760 770 780 790 800
VPENQRHGIN SQSAMVSPQA YYAGAMSMYQ CQAGPQHTPV DQMQYSPEIP
810 820 830 840 850
GSQAFLSKFQ SPSILNEAYS ADLSSIGHLQ TAAHLPRLAE AQPLPDITPS
GFL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V6M2 | G3V6M2_RAT | Aryl hydrocarbon receptor | Ahr rCG_61977 | 815 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | S → T in AAA56897 (PubMed:8065918).Curated | 1 | |
Sequence conflicti | 659 | A → V in AAA56897 (PubMed:8065918).Curated | 1 | |
Sequence conflicti | 679 – 682 | YSLF → ICLL in AAA56897 (PubMed:8065918).Curated | 4 | |
Sequence conflicti | 739 | A → V in AAA56897 (PubMed:8065918).Curated | 1 | |
Sequence conflicti | 744 | D → E in AAA56897 (PubMed:8065918).Curated | 1 | |
Sequence conflicti | 770 | A → G in AAA56897 (PubMed:8065918).Curated | 1 | |
Sequence conflicti | 787 | H → D in AAA56897 (PubMed:8065918).Curated | 1 | |
Sequence conflicti | 794 | Q → H in AAA19451 (PubMed:7812217).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 497 | V → A in strain: Han/Wistar, Han/Wistar-dv, Han/Wistar-siv and Han/Wistar-liv. 1 Publication | 1 | |
Natural varianti | 766 – 808 | Missing in strain: Han/Wistar-dv. Add BLAST | 43 | |
Natural varianti | 809 – 853 | FQSPS…PSGFL → IRAFYRE in strain: Han/Wistar-siv and Han/Wistar-liv. Add BLAST | 45 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002091 | 142 – 376 | Missing in isoform 2. 1 PublicationAdd BLAST | 235 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09000 mRNA Translation: AAA56897.1 U04860 mRNA Translation: AAA19451.1 AF082124 mRNA Translation: AAC35168.1 AF082125 mRNA Translation: AAC35169.1 AF082126 mRNA Translation: AAC35170.1 |
PIRi | S58375 |
Genome annotation databases
UCSCi | RGD:2074, rat [P41738-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09000 mRNA Translation: AAA56897.1 U04860 mRNA Translation: AAA19451.1 AF082124 mRNA Translation: AAC35168.1 AF082125 mRNA Translation: AAC35169.1 AF082126 mRNA Translation: AAC35170.1 |
PIRi | S58375 |
3D structure databases
AlphaFoldDBi | P41738 |
SMRi | P41738 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247719, 4 interactors |
IntActi | P41738, 2 interactors |
STRINGi | 10116.ENSRNOP00000006618 |
Chemistry databases
BindingDBi | P41738 |
ChEMBLi | CHEMBL5400 |
PTM databases
iPTMneti | P41738 |
PhosphoSitePlusi | P41738 |
Proteomic databases
PaxDbi | P41738 |
PRIDEi | P41738 |
Genome annotation databases
UCSCi | RGD:2074, rat [P41738-1] |
Organism-specific databases
RGDi | 2074, Ahr |
Phylogenomic databases
eggNOGi | KOG3560, Eukaryota |
InParanoidi | P41738 |
PhylomeDBi | P41738 |
Enzyme and pathway databases
Reactomei | R-RNO-211945, Phase I - Functionalization of compounds R-RNO-211976, Endogenous sterols R-RNO-211981, Xenobiotics R-RNO-8937144, Aryl hydrocarbon receptor signalling |
Miscellaneous databases
PROi | PR:P41738 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR033348, AHR IPR039091, AHR/AHRR IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 |
PANTHERi | PTHR10649, PTHR10649, 1 hit PTHR10649:SF9, PTHR10649:SF9, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit PF08447, PAS_3, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00086, PAC, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AHR_RAT | |
Accessioni | P41738Primary (citable) accession number: P41738 Secondary accession number(s): O88930, O89105 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | March 25, 2003 | |
Last modified: | May 25, 2022 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |