UniProtKB - P41734 (IAH1_YEAST)
Protein
Isoamyl acetate-hydrolyzing esterase
Gene
IAH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Plays a crucial role in the hydrolysis of isoamyl acetate in sake mash (Ref. 2). Hydrolyzes short chain esters from acetate (C2) to hexanoate (C6), showing more specificity for shorter chain exters. No activity for decanoate (C10) esters (PubMed:21069734).2 Publications
Catalytic activityi
- EC:3.1.1.1123 Publications
Kineticsi
- KM=40.3 mM for 3-mehtylbutyl acetate1 Publication
pH dependencei
Optimum pH is 7.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 12 | Nucleophile1 Publication | 1 | |
Sitei | 53 | Transition state stabilizer1 Publication | 1 | |
Sitei | 83 | Transition state stabilizer1 Publication | 1 | |
Active sitei | 187 | Proton donor1 Publication | 1 | |
Active sitei | 190 | Proton acceptor1 Publication | 1 |
GO - Molecular functioni
- hydrolase activity, acting on ester bonds Source: SGD
GO - Biological processi
- acetate metabolic process Source: SGD
- lipid catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:YOR126C-MONOMER |
SABIO-RKi | P41734 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:YOR126CImported ORF Names:O3287Imported, YOR3287CImported |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005652, IAH1 |
VEuPathDBi | FungiDB:YOR126C |
Pathology & Biotechi
Disruption phenotypei
Accumulates approximately 19 times higher amounts of isoamyl acetate compared to wild-type in laboratory scale sake brewing.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 220 – 238 | Missing : Hydrolyzes decanoate (C10) esters in contrast to wild-type. Active also on short chain esters from acetate (C2) to hexanoate (C6), but the catalytic efficiency is lower than that of wild-type. 1 PublicationAdd BLAST | 19 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084126 | 1 – 238 | Isoamyl acetate-hydrolyzing esteraseAdd BLAST | 238 |
Proteomic databases
MaxQBi | P41734 |
PaxDbi | P41734 |
PRIDEi | P41734 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 34521, 65 interactors |
STRINGi | 4932.YOR126C |
Miscellaneous databases
RNActi | P41734, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P41734 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P41734 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3035, Eukaryota |
GeneTreei | ENSGT00390000008069 |
HOGENOMi | CLU_051989_0_3_1 |
InParanoidi | P41734 |
OMAi | VPFIDMW |
Family and domain databases
Gene3Di | 3.40.50.1110, 1 hit |
InterProi | View protein in InterPro IPR013830, SGNH_hydro IPR036514, SGNH_hydro_sf |
Pfami | View protein in Pfam PF13472, Lipase_GDSL_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P41734-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDYEKFLLFG DSITEFAFNT RPIEDGKDQY ALGAALVNEY TRKMDILQRG
60 70 80 90 100
FKGYTSRWAL KILPEILKHE SNIVMATIFL GANDACSAGP QSVPLPEFID
110 120 130 140 150
NIRQMVSLMK SYHIRPIIIG PGLVDREKWE KEKSEEIALG YFRTNENFAI
160 170 180 190 200
YSDALAKLAN EEKVPFVALN KAFQQEGGDA WQQLLTDGLH FSGKGYKIFH
210 220 230
DELLKVIETF YPQYHPKNMQ YKLKDWRDVL DDGSNIMS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82930 Genomic DNA Translation: CAA58104.1 X94335 Genomic DNA Translation: CAA64045.1 X92662 Genomic DNA Translation: CAA63350.1 X90518 Genomic DNA Translation: CAA62126.1 Z75034 Genomic DNA Translation: CAA99325.1 AY557754 Genomic DNA Translation: AAS56080.1 BK006948 Genomic DNA Translation: DAA10900.1 |
PIRi | S49911 |
RefSeqi | NP_014769.3, NM_001183545.3 |
Genome annotation databases
EnsemblFungii | YOR126C_mRNA; YOR126C; YOR126C |
GeneIDi | 854293 |
KEGGi | sce:YOR126C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82930 Genomic DNA Translation: CAA58104.1 X94335 Genomic DNA Translation: CAA64045.1 X92662 Genomic DNA Translation: CAA63350.1 X90518 Genomic DNA Translation: CAA62126.1 Z75034 Genomic DNA Translation: CAA99325.1 AY557754 Genomic DNA Translation: AAS56080.1 BK006948 Genomic DNA Translation: DAA10900.1 |
PIRi | S49911 |
RefSeqi | NP_014769.3, NM_001183545.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3MIL | X-ray | 1.60 | A/B | 1-238 | [»] | |
SMRi | P41734 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34521, 65 interactors |
STRINGi | 4932.YOR126C |
Proteomic databases
MaxQBi | P41734 |
PaxDbi | P41734 |
PRIDEi | P41734 |
Genome annotation databases
EnsemblFungii | YOR126C_mRNA; YOR126C; YOR126C |
GeneIDi | 854293 |
KEGGi | sce:YOR126C |
Organism-specific databases
SGDi | S000005652, IAH1 |
VEuPathDBi | FungiDB:YOR126C |
Phylogenomic databases
eggNOGi | KOG3035, Eukaryota |
GeneTreei | ENSGT00390000008069 |
HOGENOMi | CLU_051989_0_3_1 |
InParanoidi | P41734 |
OMAi | VPFIDMW |
Enzyme and pathway databases
BioCyci | MetaCyc:YOR126C-MONOMER |
SABIO-RKi | P41734 |
Miscellaneous databases
EvolutionaryTracei | P41734 |
PROi | PR:P41734 |
RNActi | P41734, protein |
Family and domain databases
Gene3Di | 3.40.50.1110, 1 hit |
InterProi | View protein in InterPro IPR013830, SGNH_hydro IPR036514, SGNH_hydro_sf |
Pfami | View protein in Pfam PF13472, Lipase_GDSL_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IAH1_YEAST | |
Accessioni | P41734Primary (citable) accession number: P41734 Secondary accession number(s): D6W2I4, Q92313 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names