UniProtKB - P41562 (IDHC_RAT)
Protein
Isocitrate dehydrogenase [NADP] cytoplasmic
Gene
Idh1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalytic activityi
- EC:1.1.1.421 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 77 | SubstrateBy similarity | 1 | |
Binding sitei | 82 | NADPBy similarity | 1 | |
Binding sitei | 109 | SubstrateBy similarity | 1 | |
Binding sitei | 132 | SubstrateBy similarity | 1 | |
Sitei | 139 | Critical for catalysisBy similarity | 1 | |
Binding sitei | 212 | Substrate; shared with neighboring subunitBy similarity | 1 | |
Sitei | 212 | Critical for catalysisBy similarity | 1 | |
Metal bindingi | 252 | Magnesium or manganese; shared with neighboring subunitBy similarity | 1 | |
Binding sitei | 260 | NADPBy similarity | 1 | |
Metal bindingi | 275 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 279 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 328 | NADP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 75 – 77 | NADPBy similarity | 3 | |
Nucleotide bindingi | 310 – 315 | NADPBy similarity | 6 |
GO - Molecular functioni
- identical protein binding Source: RGD
- isocitrate dehydrogenase (NADP+) activity Source: RGD
- isocitrate dehydrogenase activity Source: RGD
- magnesium ion binding Source: UniProtKB
- NAD binding Source: InterPro
- NADP binding Source: RGD
- protein homodimerization activity Source: RGD
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- female gonad development Source: RGD
- glutathione metabolic process Source: RGD
- glyoxylate cycle Source: UniProtKB-KW
- isocitrate metabolic process Source: RGD
- NADP metabolic process Source: GO_Central
- regulation of phospholipid biosynthetic process Source: RGD
- regulation of phospholipid catabolic process Source: RGD
- response to organic cyclic compound Source: RGD
- response to oxidative stress Source: RGD
- response to steroid hormone Source: RGD
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Manganese, Metal-binding, NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.42, 5301 |
Reactomei | R-RNO-389542, NADPH regeneration R-RNO-6798695, Neutrophil degranulation R-RNO-9033241, Peroxisomal protein import |
SABIO-RKi | P41562 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.421 Publication)Short name: IDH Alternative name(s): Cytosolic NADP-isocitrate dehydrogenase IDP NADP(+)-specific ICDH Oxalosuccinate decarboxylase |
Gene namesi | Name:Idh1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2862, Idh1 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 2 Publications
Peroxisome
- Peroxisome 1 Publication
Cytosol
- cytosol Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Peroxisome
- peroxisome Source: HGNC-UCL
Keywords - Cellular componenti
Cytoplasm, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000083580 | 2 – 414 | Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST | 413 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 42 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 81 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 126 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 224 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 233 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 243 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 321 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 389 | PhosphoserineBy similarity | 1 | |
Modified residuei | 400 | N6-succinyllysineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked.
Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P41562 |
PaxDbi | P41562 |
PRIDEi | P41562 |
PTM databases
iPTMneti | P41562 |
PhosphoSitePlusi | P41562 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000015020, Expressed in female gonad and 20 other tissues |
Genevisiblei | P41562, RN |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
BioGRIDi | 246640, 2 interactors |
STRINGi | 10116.ENSRNOP00000020322 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 94 – 100 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family.Curated
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
GeneTreei | ENSGT00390000012547 |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | P41562 |
OMAi | HGTVQRH |
OrthoDBi | 769322at2759 |
PhylomeDBi | P41562 |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P41562-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRKIHGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEITYTPKD GSQKVTYLVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSKFE AQKIWYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT
360 370 380 390 400
ELSFFANALE EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLKAKLA QAKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L35317 mRNA Translation: AAA59356.1 |
PIRi | A54756 |
RefSeqi | NP_113698.1, NM_031510.1 XP_006245111.1, XM_006245049.3 XP_008765298.1, XM_008767076.2 |
Genome annotation databases
Ensembli | ENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020 |
GeneIDi | 24479 |
KEGGi | rno:24479 |
UCSCi | RGD:2862, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L35317 mRNA Translation: AAA59356.1 |
PIRi | A54756 |
RefSeqi | NP_113698.1, NM_031510.1 XP_006245111.1, XM_006245049.3 XP_008765298.1, XM_008767076.2 |
3D structure databases
SMRi | P41562 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246640, 2 interactors |
STRINGi | 10116.ENSRNOP00000020322 |
PTM databases
iPTMneti | P41562 |
PhosphoSitePlusi | P41562 |
Proteomic databases
jPOSTi | P41562 |
PaxDbi | P41562 |
PRIDEi | P41562 |
Genome annotation databases
Ensembli | ENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020 |
GeneIDi | 24479 |
KEGGi | rno:24479 |
UCSCi | RGD:2862, rat |
Organism-specific databases
CTDi | 3417 |
RGDi | 2862, Idh1 |
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
GeneTreei | ENSGT00390000012547 |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | P41562 |
OMAi | HGTVQRH |
OrthoDBi | 769322at2759 |
PhylomeDBi | P41562 |
Enzyme and pathway databases
BRENDAi | 1.1.1.42, 5301 |
Reactomei | R-RNO-389542, NADPH regeneration R-RNO-6798695, Neutrophil degranulation R-RNO-9033241, Peroxisomal protein import |
SABIO-RKi | P41562 |
Miscellaneous databases
PROi | PR:P41562 |
Gene expression databases
Bgeei | ENSRNOG00000015020, Expressed in female gonad and 20 other tissues |
Genevisiblei | P41562, RN |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IDHC_RAT | |
Accessioni | P41562Primary (citable) accession number: P41562 Secondary accession number(s): P80300 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families