UniProtKB - P41499 (PTN11_RAT)
Tyrosine-protein phosphatase non-receptor type 11
Ptpn11
Functioni
Catalytic activityi
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphatePROSITE-ProRule annotation1 PublicationEC:3.1.3.48PROSITE-ProRule annotation1 Publication
Activity regulationi
Kineticsi
- KM=18 mM for pNPP (isoform 1)1 Publication
- KM=48 µM for nAChR (isoform 1)1 Publication
- KM=1.3 µM for MBP (isoform 1)1 Publication
- KM=15 mM for pNPP (isoform 2)1 Publication
- KM=13 µM for nAChR (isoform 2)1 Publication
- KM=0.067 µM for MBP (isoform 2)1 Publication
- Vmax=120 µmol/min/mg enzyme toward pNPP (isoform 1)1 Publication
- Vmax=220 nmol/min/mg enzyme toward nAChR (isoform 1)1 Publication
- Vmax=210 nmol/min/mg enzyme toward MBP (isoform 1)1 Publication
- Vmax=16 µmol/min/mg enzyme toward pNPP (isoform 2)1 Publication
- Vmax=98 nmol/min/mg enzyme toward nAChR (isoform 2)1 Publication
- Vmax=15 nmol/min/mg enzyme toward MBP (isoform 2)1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 425 | SubstrateBy similarity | 1 | |
Active sitei | 459 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 | |
Binding sitei | 506 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- cadherin binding Source: RGD
- cell adhesion molecule binding Source: RGD
- D1 dopamine receptor binding Source: RGD
- insulin receptor binding Source: RGD
- insulin receptor substrate binding Source: RGD
- non-membrane spanning protein tyrosine phosphatase activity Source: UniProtKB
- peptide hormone receptor binding Source: RGD
- phospholipase binding Source: RGD
- phosphoprotein phosphatase activity Source: RGD
- phosphotyrosine residue binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase binding Source: RGD
- protein tyrosine kinase binding Source: RGD
- protein tyrosine phosphatase activity Source: RGD
- receptor tyrosine kinase binding Source: RGD
- signaling receptor complex adaptor activity Source: RGD
GO - Biological processi
- abortive mitotic cell cycle Source: RGD
- activation of MAPK activity Source: RGD
- atrioventricular canal development Source: RGD
- axonogenesis Source: RGD
- Bergmann glial cell differentiation Source: RGD
- brain development Source: RGD
- cellular response to angiotensin Source: RGD
- cellular response to epidermal growth factor stimulus Source: UniProtKB
- cellular response to hydrogen peroxide Source: RGD
- cellular response to insulin-like growth factor stimulus Source: RGD
- cellular response to mechanical stimulus Source: RGD
- cerebellar cortex formation Source: RGD
- DNA damage checkpoint Source: RGD
- ephrin receptor signaling pathway Source: UniProtKB
- epidermal growth factor receptor signaling pathway Source: RGD
- ERBB signaling pathway Source: RGD
- face morphogenesis Source: RGD
- fibroblast growth factor receptor signaling pathway Source: RGD
- genitalia development Source: RGD
- glucose homeostasis Source: RGD
- heart development Source: RGD
- homeostasis of number of cells within a tissue Source: RGD
- hormone-mediated signaling pathway Source: RGD
- hormone metabolic process Source: RGD
- inner ear development Source: RGD
- integrin-mediated signaling pathway Source: RGD
- intestinal epithelial cell migration Source: RGD
- lipid metabolic process Source: RGD
- megakaryocyte development Source: RGD
- microvillus organization Source: RGD
- multicellular organismal reproductive process Source: RGD
- multicellular organism growth Source: RGD
- negative regulation of cell adhesion mediated by integrin Source: RGD
- negative regulation of chondrocyte differentiation Source: UniProtKB
- negative regulation of cortisol secretion Source: RGD
- negative regulation of growth hormone secretion Source: RGD
- negative regulation of hormone secretion Source: RGD
- negative regulation of insulin secretion Source: RGD
- neurotrophin TRK receptor signaling pathway Source: RGD
- organ growth Source: RGD
- peptidyl-tyrosine dephosphorylation Source: UniProtKB
- platelet-derived growth factor receptor signaling pathway Source: RGD
- platelet formation Source: RGD
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of focal adhesion assembly Source: RGD
- positive regulation of glucose import Source: RGD
- positive regulation of hormone secretion Source: RGD
- positive regulation of insulin receptor signaling pathway Source: RGD
- positive regulation of interferon-beta production Source: RGD
- positive regulation of interleukin-6 production Source: RGD
- positive regulation of mitotic cell cycle Source: RGD
- positive regulation of ossification Source: UniProtKB
- positive regulation of peptidyl-tyrosine phosphorylation Source: RGD
- positive regulation of signal transduction Source: RGD
- positive regulation of tumor necrosis factor production Source: RGD
- protein dephosphorylation Source: RGD
- regulation of cell adhesion mediated by integrin Source: UniProtKB
- regulation of protein-containing complex assembly Source: RGD
- regulation of protein export from nucleus Source: RGD
- smooth muscle cell proliferation Source: RGD
- triglyceride metabolic process Source: RGD
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
Reactomei | R-RNO-1059683, Interleukin-6 signaling R-RNO-109704, PI3K Cascade R-RNO-110056, MAPK3 (ERK1) activation R-RNO-112411, MAPK1 (ERK2) activation R-RNO-114604, GPVI-mediated activation cascade R-RNO-1257604, PIP3 activates AKT signaling R-RNO-1295596, Spry regulation of FGF signaling R-RNO-1433557, Signaling by SCF-KIT R-RNO-180292, GAB1 signalosome R-RNO-186763, Downstream signal transduction R-RNO-210993, Tie2 Signaling R-RNO-388841, Costimulation by the CD28 family R-RNO-389513, CTLA4 inhibitory signaling R-RNO-389948, PD-1 signaling R-RNO-512988, Interleukin-3, Interleukin-5 and GM-CSF signaling R-RNO-5654689, PI-3K cascade:FGFR1 R-RNO-5654693, FRS-mediated FGFR1 signaling R-RNO-5654695, PI-3K cascade:FGFR2 R-RNO-5654700, FRS-mediated FGFR2 signaling R-RNO-5654706, FRS-mediated FGFR3 signaling R-RNO-5654710, PI-3K cascade:FGFR3 R-RNO-5654712, FRS-mediated FGFR4 signaling R-RNO-5654720, PI-3K cascade:FGFR4 R-RNO-5654726, Negative regulation of FGFR1 signaling R-RNO-5654727, Negative regulation of FGFR2 signaling R-RNO-5654732, Negative regulation of FGFR3 signaling R-RNO-5654733, Negative regulation of FGFR4 signaling R-RNO-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-RNO-8853659, RET signaling R-RNO-8854691, Interleukin-20 family signaling R-RNO-8865999, MET activates PTPN11 R-RNO-8934593, Regulation of RUNX1 Expression and Activity R-RNO-909733, Interferon alpha/beta signaling R-RNO-912694, Regulation of IFNA signaling R-RNO-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine-protein phosphatase non-receptor type 11 (EC:3.1.3.481 Publication)Alternative name(s): Protein-tyrosine phosphatase 1D Short name: PTP-1D Protein-tyrosine phosphatase SYP SH-PTP2 Short name: SHP-2 Short name: Shp2 |
Gene namesi | Name:Ptpn11 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3447, Ptpn11 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: RGD
- plasma membrane raft Source: RGD
Other locations
- cytoplasm Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000094769 | 2 – 593 | Tyrosine-protein phosphatase non-receptor type 11Add BLAST | 592 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineBy similarity | 1 | |
Modified residuei | 62 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 66 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 542 | Phosphotyrosine; by PDGFRBy similarity | 1 | |
Modified residuei | 580 | Phosphotyrosine; by PDGFRBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P41499 |
PaxDbi | P41499 |
PRIDEi | P41499 |
PTM databases
iPTMneti | P41499 |
PhosphoSitePlusi | P41499 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000030124, Expressed in Ammon's horn and 20 other tissues |
Genevisiblei | P41499, RN |
Interactioni
Subunit structurei
Interacts with phosphorylated SIT1, LIME1, BCAR3 and MZPL1.
Interacts with FCRL4, FCRL6, ANKHD1, SHB, INPP5D/SHIP1 and CD84 (By similarity).
Interacts with MILR1 (tyrosine-phosphorylated).
Interacts with FLT1 (tyrosine-phosphorylated), FLT3 (tyrosine-phosphorylated), FLT4 (tyrosine-phosphorylated), KIT and GRB2 (By similarity).
Interacts with PTPNS1.
Interacts with KIR2DL1; the interaction is enhanced by ARRB2.
Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).
Interacts with GAB2 (By similarity).
Interacts with TERT; the interaction retains TERT in the nucleus.
Interacts with PECAM1 and FER.
Interacts with EPHA2 (activated); participates in PTK2/FAK1 dephosphorylation in EPHA2 downstream signaling.
Interacts with PDGFRA (tyrosine phosphorylated).
Interacts with PDGFRB (tyrosine phosphorylated); this interaction increases the PTPN11 phosphatase activity (By similarity).
Interacts with ROS1; this mediates PTPN11 phosphorylation.
Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity).
Interacts with MPIG6B (via ITIM motif) (By similarity).
Interacts with SIGLEC10 (By similarity).
By similarity3 PublicationsBinary interactionsi
Hide detailsP41499
With | #Exp. | IntAct |
---|---|---|
Jak2 [Q62689] | 3 | EBI-7180604,EBI-8656708 |
Sirpa [P97710] | 3 | EBI-7180604,EBI-7945080 |
PDGFRB [P09619] from Homo sapiens. | 4 | EBI-7180604,EBI-641237 |
GO - Molecular functioni
- cadherin binding Source: RGD
- cell adhesion molecule binding Source: RGD
- D1 dopamine receptor binding Source: RGD
- insulin receptor binding Source: RGD
- insulin receptor substrate binding Source: RGD
- peptide hormone receptor binding Source: RGD
- phospholipase binding Source: RGD
- phosphotyrosine residue binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase binding Source: RGD
- protein tyrosine kinase binding Source: RGD
- receptor tyrosine kinase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247651, 5 interactors |
CORUMi | P41499 |
DIPi | DIP-47397N |
ELMi | P41499 |
IntActi | P41499, 31 interactors |
MINTi | P41499 |
STRINGi | 10116.ENSRNOP00000041842 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 102 | SH2 1PROSITE-ProRule annotationAdd BLAST | 97 | |
Domaini | 112 – 216 | SH2 2PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 247 – 517 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 271 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 459 – 465 | Substrate bindingBy similarity | 7 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, SH2 domainPhylogenomic databases
eggNOGi | KOG0790, Eukaryota |
GeneTreei | ENSGT00940000153876 |
HOGENOMi | CLU_001645_9_10_1 |
InParanoidi | P41499 |
OMAi | GKPKVTH |
OrthoDBi | 411281at2759 |
PhylomeDBi | P41499 |
TreeFami | TF351632 |
Family and domain databases
Gene3Di | 3.30.505.10, 2 hits 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR000980, SH2 IPR036860, SH2_dom_sf IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR012152, Tyr_Pase_non-rcpt_typ-6/11 IPR000387, TYR_PHOSPHATASE_dom |
Pfami | View protein in Pfam PF00017, SH2, 2 hits PF00102, Y_phosphatase, 1 hit |
PIRSFi | PIRSF000929, Tyr-Ptase_nr_6, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE PR00401, SH2DOMAIN |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit SM00252, SH2, 2 hits |
SUPFAMi | SSF52799, SSF52799, 1 hit SSF55550, SSF55550, 2 hits |
PROSITEi | View protein in PROSITE PS50001, SH2, 2 hits PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSRRWFHPN ITGVEAENLL LTRGVDGSFL ARPSKSNPGD FTLSVRRNGA
60 70 80 90 100
VTHIKIQNTG DYYDLYGGEK FATLAELVQY YMEHHGQLKE KNGDVIELKY
110 120 130 140 150
PLNCADPTSE RWFHGHLSGK EAEKLLTEKG KHGSFLVRES QSHPGDFVLS
160 170 180 190 200
VRTGDDKGES NDSKSKVTHV MIRCQELKYD VGGGERFDSL TDLVEHYKKN
210 220 230 240 250
PMVETLGTVL QLKQPLNTTR INAAEIESRV RELSKLAETT DKVKQGFWEE
260 270 280 290 300
FETLQQQECK LLYSRKEGQR QENKNKNRYK NILPFDHTRV VLHDGDPNEP
310 320 330 340 350
VSDYINANII MPEFETKCNN SKPKKSYIAT QGCLQNTVND FWRMVFQENS
360 370 380 390 400
RVIVMTTKEV ERGKSKCVKY WPDECALKEY GVMRVRNVRE SAAHDYTLRE
410 420 430 440 450
LKLSKVGQGN TERTVWQYHF RTWPDHGVPS DPGGVLDFLE EVHHKQESIV
460 470 480 490 500
DAGPVVVHCS AGIGRTGTFI VIDILIDIIR EKGVDCDIDV PKTIQMVRSQ
510 520 530 540 550
RSGMVQTEAQ YRFIYMAVQH YIETLQRRIE EEQKSKRKGH EYTNIKYSLV
560 570 580 590
DQTSGDQSPL PPCTPTPPCA EMREDSARVY ENVGLMQQQR SFR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | A → P in AAA20543 (PubMed:8048963).Curated | 1 | |
Sequence conflicti | 547 | Y → S in AAA19133 (PubMed:7512964).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060441 | 407 | G → GQALL in isoform 2. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09307 mRNA Translation: AAA20543.1 U05963 mRNA Translation: AAA19133.1 |
PIRi | A53593 |
RefSeqi | NP_001171064.1, NM_001177593.1 [P41499-1] NP_037220.2, NM_013088.2 [P41499-2] |
Genome annotation databases
Ensembli | ENSRNOT00000046323; ENSRNOP00000041842; ENSRNOG00000030124 [P41499-1] |
GeneIDi | 25622 |
KEGGi | rno:25622 |
UCSCi | RGD:3447, rat [P41499-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09307 mRNA Translation: AAA20543.1 U05963 mRNA Translation: AAA19133.1 |
PIRi | A53593 |
RefSeqi | NP_001171064.1, NM_001177593.1 [P41499-1] NP_037220.2, NM_013088.2 [P41499-2] |
3D structure databases
SMRi | P41499 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247651, 5 interactors |
CORUMi | P41499 |
DIPi | DIP-47397N |
ELMi | P41499 |
IntActi | P41499, 31 interactors |
MINTi | P41499 |
STRINGi | 10116.ENSRNOP00000041842 |
PTM databases
iPTMneti | P41499 |
PhosphoSitePlusi | P41499 |
Proteomic databases
jPOSTi | P41499 |
PaxDbi | P41499 |
PRIDEi | P41499 |
Genome annotation databases
Ensembli | ENSRNOT00000046323; ENSRNOP00000041842; ENSRNOG00000030124 [P41499-1] |
GeneIDi | 25622 |
KEGGi | rno:25622 |
UCSCi | RGD:3447, rat [P41499-2] |
Organism-specific databases
CTDi | 5781 |
RGDi | 3447, Ptpn11 |
Phylogenomic databases
eggNOGi | KOG0790, Eukaryota |
GeneTreei | ENSGT00940000153876 |
HOGENOMi | CLU_001645_9_10_1 |
InParanoidi | P41499 |
OMAi | GKPKVTH |
OrthoDBi | 411281at2759 |
PhylomeDBi | P41499 |
TreeFami | TF351632 |
Enzyme and pathway databases
Reactomei | R-RNO-1059683, Interleukin-6 signaling R-RNO-109704, PI3K Cascade R-RNO-110056, MAPK3 (ERK1) activation R-RNO-112411, MAPK1 (ERK2) activation R-RNO-114604, GPVI-mediated activation cascade R-RNO-1257604, PIP3 activates AKT signaling R-RNO-1295596, Spry regulation of FGF signaling R-RNO-1433557, Signaling by SCF-KIT R-RNO-180292, GAB1 signalosome R-RNO-186763, Downstream signal transduction R-RNO-210993, Tie2 Signaling R-RNO-388841, Costimulation by the CD28 family R-RNO-389513, CTLA4 inhibitory signaling R-RNO-389948, PD-1 signaling R-RNO-512988, Interleukin-3, Interleukin-5 and GM-CSF signaling R-RNO-5654689, PI-3K cascade:FGFR1 R-RNO-5654693, FRS-mediated FGFR1 signaling R-RNO-5654695, PI-3K cascade:FGFR2 R-RNO-5654700, FRS-mediated FGFR2 signaling R-RNO-5654706, FRS-mediated FGFR3 signaling R-RNO-5654710, PI-3K cascade:FGFR3 R-RNO-5654712, FRS-mediated FGFR4 signaling R-RNO-5654720, PI-3K cascade:FGFR4 R-RNO-5654726, Negative regulation of FGFR1 signaling R-RNO-5654727, Negative regulation of FGFR2 signaling R-RNO-5654732, Negative regulation of FGFR3 signaling R-RNO-5654733, Negative regulation of FGFR4 signaling R-RNO-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-RNO-8853659, RET signaling R-RNO-8854691, Interleukin-20 family signaling R-RNO-8865999, MET activates PTPN11 R-RNO-8934593, Regulation of RUNX1 Expression and Activity R-RNO-909733, Interferon alpha/beta signaling R-RNO-912694, Regulation of IFNA signaling R-RNO-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon |
Miscellaneous databases
PROi | PR:P41499 |
Gene expression databases
Bgeei | ENSRNOG00000030124, Expressed in Ammon's horn and 20 other tissues |
Genevisiblei | P41499, RN |
Family and domain databases
Gene3Di | 3.30.505.10, 2 hits 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR000980, SH2 IPR036860, SH2_dom_sf IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR012152, Tyr_Pase_non-rcpt_typ-6/11 IPR000387, TYR_PHOSPHATASE_dom |
Pfami | View protein in Pfam PF00017, SH2, 2 hits PF00102, Y_phosphatase, 1 hit |
PIRSFi | PIRSF000929, Tyr-Ptase_nr_6, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE PR00401, SH2DOMAIN |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit SM00252, SH2, 2 hits |
SUPFAMi | SSF52799, SSF52799, 1 hit SSF55550, SSF55550, 2 hits |
PROSITEi | View protein in PROSITE PS50001, SH2, 2 hits PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTN11_RAT | |
Accessioni | P41499Primary (citable) accession number: P41499 Secondary accession number(s): Q62626 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | December 11, 2019 | |
Last modified: | April 7, 2021 | |
This is version 192 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families