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Protein

Pyrimidine-specific ribonucleoside hydrolase RihA

Gene

rihA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes with equal efficiency cytidine or uridine to ribose and cytosine or uracil, respectively.

Miscellaneous

Strictly specific for ribonucleosides.

Enzyme regulationi

Subject to catabolite repression.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240By similarity1

GO - Molecular functioni

  • calcium ion binding Source: EcoliWiki
  • purine nucleosidase activity Source: GO_Central
  • pyrimidine-5'-nucleotide nucleosidase activity Source: EcoliWiki
  • uridine nucleosidase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6358-MONOMER
MetaCyc:G6358-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidine-specific ribonucleoside hydrolase RihA (EC:3.2.-.-)
Alternative name(s):
Cytidine/uridine-specific hydrolase
Gene namesi
Name:rihA
Synonyms:ybeK
Ordered Locus Names:b0651, JW0646
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12701 rihA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002068131 – 311Pyrimidine-specific ribonucleoside hydrolase RihAAdd BLAST311

Proteomic databases

PaxDbiP41409
PRIDEiP41409

Interactioni

Protein-protein interaction databases

BioGridi4261645, 16 interactors
IntActiP41409, 15 interactors
STRINGi316385.ECDH10B_0720

Structurei

Secondary structure

1311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi13 – 23Combined sources11
Beta strandi28 – 35Combined sources8
Beta strandi37 – 40Combined sources4
Helixi42 – 55Combined sources14
Beta strandi63 – 65Combined sources3
Beta strandi71 – 73Combined sources3
Turni79 – 81Combined sources3
Turni83 – 85Combined sources3
Helixi104 – 114Combined sources11
Beta strandi119 – 123Combined sources5
Helixi128 – 136Combined sources9
Helixi138 – 143Combined sources6
Beta strandi144 – 150Combined sources7
Beta strandi158 – 162Combined sources5
Helixi165 – 169Combined sources5
Helixi171 – 178Combined sources8
Beta strandi180 – 182Combined sources3
Beta strandi184 – 187Combined sources4
Helixi189 – 192Combined sources4
Beta strandi195 – 197Combined sources3
Helixi199 – 208Combined sources10
Helixi211 – 224Combined sources14
Beta strandi235 – 238Combined sources4
Helixi242 – 249Combined sources8
Helixi251 – 253Combined sources3
Beta strandi254 – 259Combined sources6
Beta strandi261 – 263Combined sources3
Beta strandi274 – 276Combined sources3
Beta strandi288 – 294Combined sources7
Helixi296 – 307Combined sources12
Helixi308 – 310Combined sources3

3D structure databases

ProteinModelPortaliP41409
SMRiP41409
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41409

Family & Domainsi

Sequence similaritiesi

Belongs to the IUNH family. RihA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105DU0 Bacteria
COG1957 LUCA
HOGENOMiHOG000218839
InParanoidiP41409
KOiK01250
OMAiPIWLDCD
PhylomeDBiP41409

Family and domain databases

Gene3Di3.90.245.10, 1 hit
HAMAPiMF_01431 Pyrim_hydro_RihA, 1 hit
InterProiView protein in InterPro
IPR015910 I/U_nuclsd_hydro_CS
IPR023186 Inosine/uridine_hydrolase
IPR001910 Inosine/uridine_hydrolase_dom
IPR022975 Pyrim_hydro_RihA
IPR036452 Ribo_hydro-like
PANTHERiPTHR12304 PTHR12304, 1 hit
PfamiView protein in Pfam
PF01156 IU_nuc_hydro, 1 hit
SUPFAMiSSF53590 SSF53590, 1 hit
PROSITEiView protein in PROSITE
PS01247 IUNH, 1 hit

Sequencei

Sequence statusi: Complete.

P41409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPILLDCD PGHDDAIAIV LALASPELDV KAITSSAGNQ TPEKTLRNVL
60 70 80 90 100
RMLTLLNRTD IPVAGGAVKP LMRELIIADN VHGESGLDGP ALPEPTFAPQ
110 120 130 140 150
NCTAVELMAK TLRESAEPVT IVSTGPQTNV ALLLNSHPEL HSKIARIVIM
160 170 180 190 200
GGAMGLGNWT PAAEFNIYVD PEAAEIVFQS GIPVVMAGLD VTHKAQIHVE
210 220 230 240 250
DTERFRAIGN PVSTIVAELL DFFLEYHKDE KWGFVGAPLH DPCTIAWLLK
260 270 280 290 300
PELFTSVERW VGVETQGKYT QGMTVVDYYY LTGNKPNATV MVDVDRQGFV
310
DLLADRLKFY A
Length:311
Mass (Da):33,823
Last modified:November 1, 1997 - v2
Checksum:iEEB6CF8C60BABDD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA Translation: AAB40852.1
U00096 Genomic DNA Translation: AAC73752.1
AP009048 Genomic DNA Translation: BAA35303.1
U10981 Genomic DNA No translation available.
PIRiA64800
RefSeqiNP_415184.1, NC_000913.3
WP_001207520.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73752; AAC73752; b0651
BAA35303; BAA35303; BAA35303
GeneIDi945503
KEGGiecj:JW0646
eco:b0651
PATRICifig|1411691.4.peg.1617

Similar proteinsi

Entry informationi

Entry nameiRIHA_ECOLI
AccessioniPrimary (citable) accession number: P41409
Secondary accession number(s): P77738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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