The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P41375 (IF2B_DROME)
Protein
Eukaryotic translation initiation factor 2 subunit 2
Gene
eIF2beta
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This preinitiation complex mediates ribosomal recognition of a start codon during the scanning process of the leader region.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 260 – 284 | C4-typeSequence analysisAdd BLAST | 25 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: GO_Central
- translation initiation factor activity Source: FlyBase
- translation initiation factor binding Source: GO_Central
GO - Biological processi
- axon midline choice point recognition Source: FlyBase
- formation of cytoplasmic translation initiation complex Source: GO_Central
- formation of translation preinitiation complex Source: FlyBase
- response to endoplasmic reticulum stress Source: FlyBase
- translational initiation Source: FlyBase
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-381042, PERK regulates gene expression R-DME-382556, ABC-family proteins mediated transport R-DME-72649, Translation initiation complex formation R-DME-72695, Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702, Ribosomal scanning and start codon recognition R-DME-72731, Recycling of eIF2:GDP |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 2 subunit 2Alternative name(s): Eukaryotic translation initiation factor 2 subunit beta Short name: eIF-2-beta |
Gene namesi | ORF Names:CG4153Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004926, eIF2beta |
VEuPathDBi | VectorBase:FBgn0004926 |
Subcellular locationi
Cytosol
- cytosol Source: FlyBase
Other locations
- eukaryotic 43S preinitiation complex Source: FlyBase
- eukaryotic translation initiation factor 2 complex Source: FlyBase
- multi-eIF complex Source: FlyBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000137409 | 1 – 312 | Eukaryotic translation initiation factor 2 subunit 2Add BLAST | 312 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 44 | Phosphoserine1 Publication | 1 | |
Modified residuei | 133 | Phosphoserine1 Publication | 1 | |
Modified residuei | 145 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P41375 |
PRIDEi | P41375 |
PTM databases
iPTMneti | P41375 |
SwissPalmi | P41375 |
Expressioni
Gene expression databases
Bgeei | FBgn0004926, Expressed in eye disc (Drosophila) and 39 other tissues |
ExpressionAtlasi | P41375, baseline and differential |
Genevisiblei | P41375, DM |
Interactioni
Subunit structurei
Heterotrimer composed of an alpha, a beta and a gamma chain.
GO - Molecular functioni
- translation initiation factor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 64782, 10 interactors |
DIPi | DIP-21095N |
IntActi | P41375, 1 interactor |
STRINGi | 7227.FBpp0075700 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 26 – 104 | DisorderedSequence analysisAdd BLAST | 79 | |
Regioni | 125 – 146 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 43 | Basic and acidic residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Belongs to the eIF-2-beta/eIF-5 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 260 – 284 | C4-typeSequence analysisAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2768, Eukaryota |
GeneTreei | ENSGT00390000001804 |
HOGENOMi | CLU_026663_0_1_1 |
InParanoidi | P41375 |
OMAi | CMREGNK |
OrthoDBi | 1178655at2759 |
PhylomeDBi | P41375 |
Family and domain databases
InterProi | View protein in InterPro IPR045196, IF2/IF5 IPR002735, Transl_init_fac_IF2/IF5_dom IPR016189, Transl_init_fac_IF2/IF5_N IPR016190, Transl_init_fac_IF2/IF5_Zn-bd |
PANTHERi | PTHR23001, PTHR23001, 1 hit |
Pfami | View protein in Pfam PF01873, eIF-5_eIF-2B, 1 hit |
SMARTi | View protein in SMART SM00653, eIF2B_5, 1 hit |
SUPFAMi | SSF100966, SSF100966, 1 hit SSF75689, SSF75689, 1 hit |
i Sequence
Sequence statusi: Complete.
P41375-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDAEDGFDPT LLKKKKKKKT TFDLDAALGL EDDTKKEDPQ DEASAEGGAA
60 70 80 90 100
AEEDNLDLES FGKKKKKKKK PFNMDEIEAA IPSFGGDDVA ASEEPEEEEI
110 120 130 140 150
NLDMDFSMAK KKKKSKKKEL DELFADQADD DKSEDKENDE DNSSTWFGSD
160 170 180 190 200
RDYTYDELLK RVFEIILDKN PDMAAGRKPK FVMRPPQVLR VGTKKTSFAN
210 220 230 240 250
FMDIAKTLHR LPKHLLDFLL AELGTSGSMD GNQQLIIKGR FQPKQIENVL
260 270 280 290 300
RRYIKEYVTC HTCRSPETIL QKDTRLFFLQ CESCGSRCSV ASIKSGFQAV
310
TGKRAAIRAK TT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | S → G in AAL48875 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 64 | K → E in AAL48875 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19197 mRNA Translation: AAA28504.1 AE014296 Genomic DNA Translation: AAF49902.1 AY071253 mRNA Translation: AAL48875.1 |
RefSeqi | NP_524043.1, NM_079319.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0075968; FBpp0075700; FBgn0004926 |
GeneIDi | 39433 |
KEGGi | dme:Dmel_CG4153 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19197 mRNA Translation: AAA28504.1 AE014296 Genomic DNA Translation: AAF49902.1 AY071253 mRNA Translation: AAL48875.1 |
RefSeqi | NP_524043.1, NM_079319.4 |
3D structure databases
AlphaFoldDBi | P41375 |
SMRi | P41375 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 64782, 10 interactors |
DIPi | DIP-21095N |
IntActi | P41375, 1 interactor |
STRINGi | 7227.FBpp0075700 |
PTM databases
iPTMneti | P41375 |
SwissPalmi | P41375 |
Proteomic databases
PaxDbi | P41375 |
PRIDEi | P41375 |
Genome annotation databases
EnsemblMetazoai | FBtr0075968; FBpp0075700; FBgn0004926 |
GeneIDi | 39433 |
KEGGi | dme:Dmel_CG4153 |
Organism-specific databases
CTDi | 39433 |
FlyBasei | FBgn0004926, eIF2beta |
VEuPathDBi | VectorBase:FBgn0004926 |
Phylogenomic databases
eggNOGi | KOG2768, Eukaryota |
GeneTreei | ENSGT00390000001804 |
HOGENOMi | CLU_026663_0_1_1 |
InParanoidi | P41375 |
OMAi | CMREGNK |
OrthoDBi | 1178655at2759 |
PhylomeDBi | P41375 |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-381042, PERK regulates gene expression R-DME-382556, ABC-family proteins mediated transport R-DME-72649, Translation initiation complex formation R-DME-72695, Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702, Ribosomal scanning and start codon recognition R-DME-72731, Recycling of eIF2:GDP |
Miscellaneous databases
BioGRID-ORCSi | 39433, 0 hits in 1 CRISPR screen |
GenomeRNAii | 39433 |
PROi | PR:P41375 |
Gene expression databases
Bgeei | FBgn0004926, Expressed in eye disc (Drosophila) and 39 other tissues |
ExpressionAtlasi | P41375, baseline and differential |
Genevisiblei | P41375, DM |
Family and domain databases
InterProi | View protein in InterPro IPR045196, IF2/IF5 IPR002735, Transl_init_fac_IF2/IF5_dom IPR016189, Transl_init_fac_IF2/IF5_N IPR016190, Transl_init_fac_IF2/IF5_Zn-bd |
PANTHERi | PTHR23001, PTHR23001, 1 hit |
Pfami | View protein in Pfam PF01873, eIF-5_eIF-2B, 1 hit |
SMARTi | View protein in SMART SM00653, eIF2B_5, 1 hit |
SUPFAMi | SSF100966, SSF100966, 1 hit SSF75689, SSF75689, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IF2B_DROME | |
Accessioni | P41375Primary (citable) accession number: P41375 Secondary accession number(s): Q8SYX9, Q9VTZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Translation initiation factors
List of translation initiation factor entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families