UniProtKB - P41318 (LST8_YEAST)
Protein
Target of rapamycin complex subunit LST8
Gene
LST8
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle-dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity.3 Publications
Miscellaneous
Present with 589 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- protein kinase activator activity Source: SGD
GO - Biological processi
- establishment or maintenance of actin cytoskeleton polarity Source: SGD
- fungal-type cell wall organization Source: SGD
- mitochondria-nucleus signaling pathway Source: SGD
- regulation of actin cytoskeleton organization Source: GO_Central
- regulation of cell growth Source: SGD
- signal transduction Source: SGD
- TOR signaling Source: SGD
Enzyme and pathway databases
Reactomei | R-SCE-1257604, PIP3 activates AKT signaling R-SCE-3371571, HSF1-dependent transactivation R-SCE-389357, CD28 dependent PI3K/Akt signaling R-SCE-5218920, VEGFR2 mediated vascular permeability R-SCE-6804757, Regulation of TP53 Degradation R-SCE-9639288, Amino acids regulate mTORC1 |
Names & Taxonomyi
Protein namesi | Recommended name: Target of rapamycin complex subunit LST8Short name: TORC subunit LST8 Alternative name(s): Lethal with SEC13 protein 8 |
Gene namesi | Name:LST8 Ordered Locus Names:YNL006W ORF Names:N2005 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004951, LST8 |
VEuPathDBi | FungiDB:YNL006W |
Subcellular locationi
Plasma membrane
Vacuole
Note: Also localizes to membranous structures both proximal to, yet distinct from, the plasma membrane as well as within the cell interior, probably endosomal or Golgi membranes.
Cytosol
- cytosol Source: SGD
Endosome
- endosome membrane Source: SGD
Golgi apparatus
- Golgi membrane Source: SGD
Nucleus
- nuclear periphery Source: SGD
Plasma Membrane
Vacuole
- vacuolar membrane Source: UniProtKB-SubCell
Other locations
- TORC1 complex Source: SGD
- TORC2 complex Source: SGD
Keywords - Cellular componenti
Cell membrane, Membrane, VacuolePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 138 | G → D in LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication | 1 | |
Mutagenesisi | 146 | G → E in LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication | 1 | |
Mutagenesisi | 171 | G → E in LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication | 1 | |
Mutagenesisi | 181 | G → E in LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication | 1 | |
Mutagenesisi | 300 | L → S in LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051483 | 1 – 303 | Target of rapamycin complex subunit LST8Add BLAST | 303 |
Proteomic databases
MaxQBi | P41318 |
PaxDbi | P41318 |
PRIDEi | P41318 |
Interactioni
Subunit structurei
The target of rapamycin complex 1 (TORC1) is composed of at least KOG1, LST8, TCO89 and either TOR1 (TORC1-A) or TOR2 (TORC1-B). TORC1 binds to and is inhibited by FKBP-rapamycin. The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. LST8 binds to the C-terminal kinase domain in TOR2.
4 PublicationsBinary interactionsi
Hide detailsP41318
Protein-protein interaction databases
BioGRIDi | 35819, 731 interactors |
ComplexPortali | CPX-1715, TORC1 serine/threonine-protein kinase complex, TOR1 variant CPX-1716, TORC1 serine/threonine-protein kinase complex, TOR2 variant CPX-1717, TORC2 complex |
DIPi | DIP-5318N |
IntActi | P41318, 39 interactors |
MINTi | P41318 |
STRINGi | 4932.YNL006W |
Miscellaneous databases
RNActi | P41318, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 1 – 27 | WD 1Add BLAST | 27 | |
Repeati | 30 – 68 | WD 2Add BLAST | 39 | |
Repeati | 73 – 112 | WD 3Add BLAST | 40 | |
Repeati | 114 – 153 | WD 4Add BLAST | 40 | |
Repeati | 157 – 196 | WD 5Add BLAST | 40 | |
Repeati | 205 – 244 | WD 6Add BLAST | 40 | |
Repeati | 248 – 287 | WD 7Add BLAST | 40 |
Sequence similaritiesi
Belongs to the WD repeat LST8 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0315, Eukaryota |
GeneTreei | ENSGT00390000014795 |
HOGENOMi | CLU_000288_57_5_1 |
InParanoidi | P41318 |
OMAi | LISCDQA |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR037588, MLST8 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR19842, PTHR19842, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P41318-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA
60 70 80 90 100
GHQNVRLYDI RTTNPNPVAS FEGHRGNVTS VSFQQDNRWM VTSSEDGTIK
110 120 130 140 150
VWDVRSPSIP RNYKHNAPVN EVVIHPNQGE LISCDRDGNI RIWDLGENQC
160 170 180 190 200
THQLTPEDDT SLQSLSMASD GSMLAAANTK GNCYVWEMPN HTDASHLKPV
210 220 230 240 250
TKFRAHSTYI TRILLSSDVK HLATCSADHT ARVWSIDDDF KLETTLDGHQ
260 270 280 290 300
RWVWDCAFSA DSAYLVTASS DHYVRLWDLS TREIVRQYGG HHKGAVCVAL
NDV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77114 Genomic DNA Translation: CAA54380.1 Z71282 Genomic DNA Translation: CAA95865.1 BK006947 Genomic DNA Translation: DAA10537.1 |
PIRi | S45461 |
RefSeqi | NP_014392.3, NM_001182845.3 |
Genome annotation databases
EnsemblFungii | YNL006W_mRNA; YNL006W; YNL006W |
GeneIDi | 855726 |
KEGGi | sce:YNL006W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77114 Genomic DNA Translation: CAA54380.1 Z71282 Genomic DNA Translation: CAA95865.1 BK006947 Genomic DNA Translation: DAA10537.1 |
PIRi | S45461 |
RefSeqi | NP_014392.3, NM_001182845.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EMK | electron microscopy | 8.00 | B/D | 1-303 | [»] | |
SMRi | P41318 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35819, 731 interactors |
ComplexPortali | CPX-1715, TORC1 serine/threonine-protein kinase complex, TOR1 variant CPX-1716, TORC1 serine/threonine-protein kinase complex, TOR2 variant CPX-1717, TORC2 complex |
DIPi | DIP-5318N |
IntActi | P41318, 39 interactors |
MINTi | P41318 |
STRINGi | 4932.YNL006W |
Proteomic databases
MaxQBi | P41318 |
PaxDbi | P41318 |
PRIDEi | P41318 |
Genome annotation databases
EnsemblFungii | YNL006W_mRNA; YNL006W; YNL006W |
GeneIDi | 855726 |
KEGGi | sce:YNL006W |
Organism-specific databases
SGDi | S000004951, LST8 |
VEuPathDBi | FungiDB:YNL006W |
Phylogenomic databases
eggNOGi | KOG0315, Eukaryota |
GeneTreei | ENSGT00390000014795 |
HOGENOMi | CLU_000288_57_5_1 |
InParanoidi | P41318 |
OMAi | LISCDQA |
Enzyme and pathway databases
Reactomei | R-SCE-1257604, PIP3 activates AKT signaling R-SCE-3371571, HSF1-dependent transactivation R-SCE-389357, CD28 dependent PI3K/Akt signaling R-SCE-5218920, VEGFR2 mediated vascular permeability R-SCE-6804757, Regulation of TP53 Degradation R-SCE-9639288, Amino acids regulate mTORC1 |
Miscellaneous databases
PROi | PR:P41318 |
RNActi | P41318, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR037588, MLST8 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR19842, PTHR19842, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LST8_YEAST | |
Accessioni | P41318Primary (citable) accession number: P41318 Secondary accession number(s): D6W1H1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families