UniProtKB - P41266 (GP_PUUMP)
Envelopment polyprotein
GP
Functioni
Glycoprotein N and Glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).
By similarityGO - Biological processi
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- signal transduction Source: InterPro
- suppression by virus of host innate immune response Source: UniProtKB-KW
- suppression by virus of host TRAF activity Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Envelopment polyproteinAlternative name(s): M polyprotein Cleaved into the following 2 chains: Alternative name(s): Glycoprotein G1 Alternative name(s): Glycoprotein G2 |
Gene namesi | Name:GP |
Organismi | Puumala virus (strain P360) |
Taxonomic identifieri | 39001 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Ellioviricetes › Bunyavirales › Hantaviridae › Mammantavirinae › Orthohantavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135] |
Subcellular locationi
- Virion membrane By similarity
- Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
- Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein N to the Golgi complex, where virion budding occurs.By similarity
- Virion membrane By similarity
- Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 496 | LumenalSequence analysisAdd BLAST | 473 | |
Transmembranei | 497 – 517 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 518 – 658 | CytoplasmicSequence analysisAdd BLAST | 141 | |
Topological domaini | 659 – 1115 | LumenalSequence analysisAdd BLAST | 457 | |
Transmembranei | 1116 – 1136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1137 – 1148 | CytoplasmicSequence analysisAdd BLAST | 12 |
Keywords - Cellular componenti
Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000036828 | 24 – 1148 | Envelopment polyproteinAdd BLAST | 1125 | |
ChainiPRO_0000036829 | 24 – 658 | Glycoprotein NBy similarityAdd BLAST | 635 | |
ChainiPRO_0000036830 | 659 – 1148 | Glycoprotein CBy similarityAdd BLAST | 490 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 142 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 357 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 409 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 898 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 937 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 658 – 659 | Cleavage; by host signal peptidaseBy similarity | 2 |
Keywords - PTMi
GlycoproteinInteractioni
Subunit structurei
Glycoprotein N and Glycoprotein C interacts with each other.
By similarityStructurei
Secondary structure
3D structure databases
SMRi | P41266 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 621 – 644 | ITAMPROSITE-ProRule annotationAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR016402, Envelope_glycoprot_Hantavirus IPR002534, Hanta_G1 IPR002532, Hanta_G2 IPR012316, ITAM_motif_hantavir-typ |
Pfami | View protein in Pfam PF01567, Hanta_G1, 1 hit PF01561, Hanta_G2, 1 hit PF10538, ITAM_Cys-rich, 1 hit |
PIRSFi | PIRSF003945, M_poly_HantaV, 1 hit |
PROSITEi | View protein in PROSITE PS51056, ITAM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGELSPVCLC LLLQGLLLCN TGAARNLNEL KMECPHTIRL GQGLVVGSVE
60 70 80 90 100
LPSLPIQQVE TLKLESSCNF DLHTSTAGQQ SFTKWTWEIK GDLAENTQAS
110 120 130 140 150
STSFQTKSSE VNLRGLCLIP TLVVETAARM RKTIACYDLS CNQTVCQPTV
160 170 180 190 200
YLMGPIQTCI TTKSCLLSLG DQRIQVNYEK TYCVSGQLVE GICFNPIHTM
210 220 230 240 250
ALSQPSHTYD IMTMMVRCFL VIKKVTSGDS MKIEKNFETL VQKNGCTANN
260 270 280 290 300
FQGYYICLIG SSSEPLYVPA LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS
310 320 330 340 350
AMRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTS KDDPVYIWAP
360 370 380 390 400
GIIMEGNHSI CEKKTLPLTW TGFISLPGEI EKTTQCTVFC TLAGPGADCE
410 420 430 440 450
AYSETGIFNI SSPTCLINRV QRFRGSEQQI KFVCQRVDMD ITVYCNGMKK
460 470 480 490 500
VILTKTLVIG QCIYTFTSIF SLIPGVAHSL AVELCVPGLH GWATMLLLLT
510 520 530 540 550
FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILIE KVKREYQKTM
560 570 580 590 600
GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT TSALQAHFKV
610 620 630 640 650
CKLTSRFQEN LRKSLTVYEP MQGCYRTLSL FRYRSRFFVG LVWCVLLVLE
660 670 680 690 700
LIVWAASAET QNLNAGWTDT AHGSGIIPMK TDLELDFSLP SSASYTYRRQ
710 720 730 740 750
LQNPANEQEK IPFHLQLSKQ VIHAEIQHLG HWMDATFNLK TAFHCYGSCE
760 770 780 790 800
KYAYPWQTAG CFIEKDYEYE TGWGCNPPDC PGVGTGCTAC GVYLDKLKSV
810 820 830 840 850
GKVFKIVSLR YTRKVCIQLG TEQTCKTVDS NDCLITTSVK VCLIGTISKF
860 870 880 890 900
QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS
910 920 930 940 950
FRKKCAFATT PVCQFDGNTI SGYKRMIATK DSFQSFNVTE PHISTSALEW
960 970 980 990 1000
IDPDSSLRDH INVIVSRDLS FQDLSETPCQ IDLATASIDG AWGSGVGFNL
1010 1020 1030 1040 1050
VCTVSLTECS AFLTSIKACD AAMCYGSTTA NLVRGQNTIH IVGKGGHSGS
1060 1070 1080 1090 1100
KFMCCHDTKC SSTGLVAAAP HLDRVTGYNQ ADSDKIFDDG APECGMSCWF
1110 1120 1130 1140
KKSGEWILGV LNGNWMVVAV LVVLLILSIL LFTLCCPRRP SYRKEHKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08755 Unassigned RNA Translation: AAC37848.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08755 Unassigned RNA Translation: AAC37848.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5J81 | X-ray | 1.80 | A | 659-1106 | [»] | |
5J9H | X-ray | 2.50 | A | 659-1106 | [»] | |
SMRi | P41266 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR016402, Envelope_glycoprot_Hantavirus IPR002534, Hanta_G1 IPR002532, Hanta_G2 IPR012316, ITAM_motif_hantavir-typ |
Pfami | View protein in Pfam PF01567, Hanta_G1, 1 hit PF01561, Hanta_G2, 1 hit PF10538, ITAM_Cys-rich, 1 hit |
PIRSFi | PIRSF003945, M_poly_HantaV, 1 hit |
PROSITEi | View protein in PROSITE PS51056, ITAM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GP_PUUMP | |
Accessioni | P41266Primary (citable) accession number: P41266 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | June 2, 2021 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families