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Entry version 101 (11 Dec 2019)
Sequence version 1 (01 Feb 1995)
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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 7.7 sec(-1).1 Publication
  1. KM=0.13 mM for ATP1 Publication
  2. KM=26.8 µM for tyrosine1 Publication
  3. KM=2.54 µM for tRNA(Tyr)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei234ATPUniRule annotation1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    P41256

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation2 Publications)
    Alternative name(s):
    TyrRZ1 Publication
    Tyrosyl-tRNA synthetase1 PublicationUniRule annotation
    Short name:
    TyrRS1 PublicationUniRule annotation
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:tyrSUniRule annotation
    Synonyms:tyrZ1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri920 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaAcidithiobacilliaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi53H → A: Does not complement the tyrS mutation in E.coli. 1 Publication1
    Mutagenesisi306H → A: Does not complement the tyrS mutation in E.coli. 3-fold decrease in Kcat for amino acid activation, without effect on Km for tyrosine or ATP. 1 Publication1
    Mutagenesisi306H → D: Does not complement the tyrS mutation in E.coli. 1 Publication1
    Mutagenesisi356S → A: Does not complement the tyrS mutation in E.coli. 7-fold increase in Km for E.coli tRNA, without effect on other kinetic parameters. 1 Publication1
    Mutagenesisi395K → N: Complements the tyrS mutation in E. coli very poorly. 17-fold increase in Km for E.coli tRNA, without effect on Km for ATP or tyrosine. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000556661 – 407Tyrosine--tRNA ligaseAdd BLAST407

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    UniRule annotation1 Publication

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P41256

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini342 – 403S4 RNA-bindingUniRule annotationAdd BLAST62

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi47 – 56'HIGH' regionUniRule annotation10
    Motifi231 – 235'KMSKS' regionUniRule annotation5

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.UniRule annotationCurated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105DA0 Bacteria
    COG0162 LUCA

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00165 S4, 1 hit
    cd00805 TyrRS_core, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.10.290.10, 1 hit
    3.40.50.620, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_02007 Tyr_tRNA_synth_type2, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR002305 aa-tRNA-synth_Ic
    IPR014729 Rossmann-like_a/b/a_fold
    IPR002942 S4_RNA-bd
    IPR036986 S4_RNA-bd_sf
    IPR002307 Tyr-tRNA-ligase
    IPR024088 Tyr-tRNA-ligase_bac-type
    IPR024108 Tyr-tRNA-ligase_bac_2

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR11766 PTHR11766, 1 hit
    PTHR11766:SF1 PTHR11766:SF1, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00579 tRNA-synt_1b, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR01040 TRNASYNTHTYR

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00363 S4, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00234 tyrS, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS50889 S4, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P41256-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTMKHQDAFE QIAFGTVDML PEGEMLARLA AAQRDNRPLR IKLGMDPTAP
    60 70 80 90 100
    DLHLGAYVLL HKARQFQDLG HRLLFVIGDF TAMIGDPTGK SVTRKALSRE
    110 120 130 140 150
    EVVANAATYR PQVFKILDPE RTEVMFNSEW LGALRPEELI QIAACYTVAR
    160 170 180 190 200
    MLERDDFNKR YSANQPIAIH EFLYPLLQGY DSVAIKADVE LGGTDQRFNL
    210 220 230 240 250
    LVGRELQREY GQKPQLVLTM PILEGLDGVQ KMSKSLGNFI AVEDPPAEMF
    260 270 280 290 300
    GKIMSISDFL MWRYYALLSR VPAVEQTRLQ KEAASGARNP RDIKLDLAGE
    310 320 330 340 350
    LVRRFHGTAA AQEAHIAFLA RFQRHETPED LPLQAIKLSE APRLSQLLVQ
    360 370 380 390 400
    VHLAASTSEA MRKMKEGAVR VDWRRVVDPA TILALDAVYL LQFGKRHFAR

    VALQKGE
    Length:407
    Mass (Da):45,860
    Last modified:February 1, 1995 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7B73FBA2252A2C6B
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    X79010 Genomic DNA Translation: CAA55643.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    A55515

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X79010 Genomic DNA Translation: CAA55643.1
    PIRiA55515

    3D structure databases

    SMRiP41256
    ModBaseiSearch...

    Phylogenomic databases

    eggNOGiENOG4105DA0 Bacteria
    COG0162 LUCA

    Enzyme and pathway databases

    SABIO-RKiP41256

    Family and domain databases

    CDDicd00165 S4, 1 hit
    cd00805 TyrRS_core, 1 hit
    Gene3Di3.10.290.10, 1 hit
    3.40.50.620, 1 hit
    HAMAPiMF_02007 Tyr_tRNA_synth_type2, 1 hit
    InterProiView protein in InterPro
    IPR002305 aa-tRNA-synth_Ic
    IPR014729 Rossmann-like_a/b/a_fold
    IPR002942 S4_RNA-bd
    IPR036986 S4_RNA-bd_sf
    IPR002307 Tyr-tRNA-ligase
    IPR024088 Tyr-tRNA-ligase_bac-type
    IPR024108 Tyr-tRNA-ligase_bac_2
    PANTHERiPTHR11766 PTHR11766, 1 hit
    PTHR11766:SF1 PTHR11766:SF1, 1 hit
    PfamiView protein in Pfam
    PF00579 tRNA-synt_1b, 1 hit
    PRINTSiPR01040 TRNASYNTHTYR
    SMARTiView protein in SMART
    SM00363 S4, 1 hit
    TIGRFAMsiTIGR00234 tyrS, 1 hit
    PROSITEiView protein in PROSITE
    PS50889 S4, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYY_ACIFR
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P41256
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: December 11, 2019
    This is version 101 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
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