UniProtKB - P41208 (CETN2_HUMAN)
Centrin-2
CETN2
Functioni
Plays a fundamental role in microtubule organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CCP110.
Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores.
1 Publication1 PublicationMiscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 41 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 43 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 45 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 47 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 52 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 150 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 152 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 154 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 156 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 161 | Calcium 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium ion binding Source: GO_Central
- G-protein beta/gamma-subunit complex binding Source: Ensembl
- heterotrimeric G-protein binding Source: Ensembl
- microtubule binding Source: Ensembl
GO - Biological processi
- cell division Source: UniProtKB-KW
- centriole replication Source: UniProtKB
- mitotic cell cycle Source: GO_Central
- mRNA transport Source: UniProtKB-KW
- nucleotide-excision repair Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of cytokinesis Source: UniProtKB
- spermatogenesis Source: Ensembl
Keywordsi
Biological process | Cell cycle, Cell division, DNA damage, DNA repair, Mitosis, mRNA transport, Protein transport, Translocation, Transport |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P41208 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-5696394, DNA Damage Recognition in GG-NER R-HSA-5696395, Formation of Incision Complex in GG-NER R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | P41208 |
SIGNORi | P41208 |
Names & Taxonomyi
Protein namesi | Recommended name: Centrin-2Alternative name(s): Caltractin isoform 1 |
Gene namesi | Name:CETN2 Synonyms:CALT, CEN2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1867, CETN2 |
MIMi | 300006, gene |
neXtProti | NX_P41208 |
VEuPathDBi | HostDB:ENSG00000147400 |
Subcellular locationi
Cytoskeleton
- centrosome 1 Publication
- centriole 1 Publication
Nucleus
- Nucleus envelope 1 Publication
- nuclear pore complex 1 Publication
- Nucleus Curated
Cytoskeleton
- centriole Source: UniProtKB
- centrosome Source: UniProtKB
- ciliary basal body Source: Ensembl
Cytosol
- cytosol Source: Reactome
Nucleus
- nuclear pore nuclear basket Source: UniProtKB
- nucleoplasm Source: Reactome
- transcription export complex 2 Source: UniProtKB
- XPC complex Source: UniProtKB
Other locations
- 9+2 motile cilium Source: Ensembl
- apical part of cell Source: Ensembl
- photoreceptor connecting cilium Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Nuclear pore complex, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 1069 |
OpenTargetsi | ENSG00000147400 |
PharmGKBi | PA26420 |
Miscellaneous databases
Pharosi | P41208, Tbio |
Genetic variation databases
BioMutai | CETN2 |
DMDMi | 729052 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000073561 | 2 – 172 | Centrin-2Add BLAST | 171 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Cross-linki | 22 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 26 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P41208 |
jPOSTi | P41208 |
MassIVEi | P41208 |
MaxQBi | P41208 |
PaxDbi | P41208 |
PeptideAtlasi | P41208 |
PRIDEi | P41208 |
ProteomicsDBi | 55415 |
TopDownProteomicsi | P41208 |
PTM databases
iPTMneti | P41208 |
MetOSitei | P41208 |
PhosphoSitePlusi | P41208 |
Expressioni
Gene expression databases
Bgeei | ENSG00000147400, Expressed in bronchial epithelial cell and 242 other tissues |
Genevisiblei | P41208, HS |
Organism-specific databases
HPAi | ENSG00000147400, Tissue enhanced (fallopian) |
Interactioni
Subunit structurei
Monomer. Homooligomer (PubMed:15356003).
Interacts with SFI1 (PubMed:16956364).
Interacts with CCP110 (PubMed:16760425).
Component of the XPC complex composed of XPC, RAD23B and CETN2 (PubMed:11279143, PubMed:15964821, PubMed:16533048, PubMed:16627479).
Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, 2 copies of ENY2, PCID2, SEM1/DSS1, and either centrin CETN2 or centrin CETN3. The TREX-2 complex also associates with ALYREF/ALY and with the nucleoporin NUP153 (PubMed:22307388, PubMed:23591820).
9 PublicationsBinary interactionsi
P41208
GO - Molecular functioni
- microtubule binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 107496, 100 interactors |
IntActi | P41208, 59 interactors |
STRINGi | 9606.ENSP00000359300 |
Miscellaneous databases
RNActi | P41208, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P41208 |
SMRi | P41208 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P41208 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 63 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 64 – 99 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 101 – 136 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 137 – 172 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 30 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 2 – 25 | Required for self-assemblyAdd BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 19 | Polar residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0028, Eukaryota |
GeneTreei | ENSGT00940000155935 |
HOGENOMi | CLU_061288_18_2_1 |
InParanoidi | P41208 |
OMAi | YDEFEYM |
OrthoDBi | 1382571at2759 |
PhylomeDBi | P41208 |
TreeFami | TF101141 |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
DisProti | DP01259 |
IDEALi | IID00551 |
InterProi | View protein in InterPro IPR037629, Centrin_2 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR000629, RNA-helicase_DEAD-box_CS |
PANTHERi | PTHR23050:SF305, PTHR23050:SF305, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 2 hits |
SMARTi | View protein in SMART SM00054, EFh, 4 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MASNFKKANM ASSSQRKRMS PKPELTEEQK QEIREAFDLF DADGTGTIDV
60 70 80 90 100
KELKVAMRAL GFEPKKEEIK KMISEIDKEG TGKMNFGDFL TVMTQKMSEK
110 120 130 140 150
DTKEEILKAF KLFDDDETGK ISFKNLKRVA KELGENLTDE ELQEMIDEAD
160 170
RDGDGEVSEQ EFLRIMKKTS LY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72964 mRNA Translation: CAA51467.1 U82671 Genomic DNA No translation available. AK311940 mRNA Translation: BAG34881.1 BT007256 mRNA Translation: AAP35920.1 AY919675 Genomic DNA Translation: AAW82436.1 CH471172 Genomic DNA Translation: EAW72900.1 BC005334 mRNA Translation: AAH05334.1 BC013873 mRNA Translation: AAH13873.1 |
CCDSi | CCDS14716.1 |
PIRi | A49652 |
RefSeqi | NP_004335.1, NM_004344.1 |
Genome annotation databases
Ensembli | ENST00000370277.5; ENSP00000359300.3; ENSG00000147400.10 |
GeneIDi | 1069 |
KEGGi | hsa:1069 |
MANE-Selecti | ENST00000370277.5; ENSP00000359300.3; NM_004344.3; NP_004335.1 |
UCSCi | uc004fgq.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72964 mRNA Translation: CAA51467.1 U82671 Genomic DNA No translation available. AK311940 mRNA Translation: BAG34881.1 BT007256 mRNA Translation: AAP35920.1 AY919675 Genomic DNA Translation: AAW82436.1 CH471172 Genomic DNA Translation: EAW72900.1 BC005334 mRNA Translation: AAH05334.1 BC013873 mRNA Translation: AAH13873.1 |
CCDSi | CCDS14716.1 |
PIRi | A49652 |
RefSeqi | NP_004335.1, NM_004344.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M39 | NMR | - | A | 84-172 | [»] | |
1ZMZ | NMR | - | A | 1-98 | [»] | |
2A4J | NMR | - | A | 94-172 | [»] | |
2GGM | X-ray | 2.35 | A/B | 1-172 | [»] | |
2K2I | NMR | - | A | 94-172 | [»] | |
2OBH | X-ray | 1.80 | A/B | 26-168 | [»] | |
AlphaFoldDBi | P41208 | |||||
SMRi | P41208 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107496, 100 interactors |
IntActi | P41208, 59 interactors |
STRINGi | 9606.ENSP00000359300 |
PTM databases
iPTMneti | P41208 |
MetOSitei | P41208 |
PhosphoSitePlusi | P41208 |
Genetic variation databases
BioMutai | CETN2 |
DMDMi | 729052 |
Proteomic databases
EPDi | P41208 |
jPOSTi | P41208 |
MassIVEi | P41208 |
MaxQBi | P41208 |
PaxDbi | P41208 |
PeptideAtlasi | P41208 |
PRIDEi | P41208 |
ProteomicsDBi | 55415 |
TopDownProteomicsi | P41208 |
Protocols and materials databases
Antibodypediai | 30770, 197 antibodies from 28 providers |
DNASUi | 1069 |
Genome annotation databases
Ensembli | ENST00000370277.5; ENSP00000359300.3; ENSG00000147400.10 |
GeneIDi | 1069 |
KEGGi | hsa:1069 |
MANE-Selecti | ENST00000370277.5; ENSP00000359300.3; NM_004344.3; NP_004335.1 |
UCSCi | uc004fgq.4, human |
Organism-specific databases
CTDi | 1069 |
DisGeNETi | 1069 |
GeneCardsi | CETN2 |
HGNCi | HGNC:1867, CETN2 |
HPAi | ENSG00000147400, Tissue enhanced (fallopian) |
MIMi | 300006, gene |
neXtProti | NX_P41208 |
OpenTargetsi | ENSG00000147400 |
PharmGKBi | PA26420 |
VEuPathDBi | HostDB:ENSG00000147400 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0028, Eukaryota |
GeneTreei | ENSGT00940000155935 |
HOGENOMi | CLU_061288_18_2_1 |
InParanoidi | P41208 |
OMAi | YDEFEYM |
OrthoDBi | 1382571at2759 |
PhylomeDBi | P41208 |
TreeFami | TF101141 |
Enzyme and pathway databases
PathwayCommonsi | P41208 |
Reactomei | R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-5696394, DNA Damage Recognition in GG-NER R-HSA-5696395, Formation of Incision Complex in GG-NER R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | P41208 |
SIGNORi | P41208 |
Miscellaneous databases
BioGRID-ORCSi | 1069, 13 hits in 703 CRISPR screens |
ChiTaRSi | CETN2, human |
EvolutionaryTracei | P41208 |
GeneWikii | CETN2 |
GenomeRNAii | 1069 |
Pharosi | P41208, Tbio |
PROi | PR:P41208 |
RNActi | P41208, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000147400, Expressed in bronchial epithelial cell and 242 other tissues |
Genevisiblei | P41208, HS |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
DisProti | DP01259 |
IDEALi | IID00551 |
InterProi | View protein in InterPro IPR037629, Centrin_2 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR000629, RNA-helicase_DEAD-box_CS |
PANTHERi | PTHR23050:SF305, PTHR23050:SF305, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 2 hits |
SMARTi | View protein in SMART SM00054, EFh, 4 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CETN2_HUMAN | |
Accessioni | P41208Primary (citable) accession number: P41208 Secondary accession number(s): B2R4T4, Q53XW1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 214 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families