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Protein

DNA-binding protein inhibitor ID-2

Gene

Id2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-2
Alternative name(s):
Inhibitor of DNA binding 2
Inhibitor of differentiation 2
Gene namesi
Name:Id2
Synonyms:Id-2, Idb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:96397 Id2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit disrupted locomotor rhythms, reduced locomotor activity, enhanced phase shifts and an increased rate of entrainment when subjected to a large delay of the photoschedule. Show a reduction in lipid storage in the liver and white adipose tissue.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272421 – 134DNA-binding protein inhibitor ID-2Add BLAST134

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei25PhosphoserineCombined sources1

Post-translational modificationi

Polyubiquitinated; which is favored by Ifi204 and leads to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP41136
PeptideAtlasiP41136
PRIDEiP41136

PTM databases

iPTMnetiP41136
PhosphoSitePlusiP41136

Expressioni

Inductioni

Expressed in a circadian manner in the liver with peak levels seen at CT12 (at protein level). Expressed in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and heart with peak levels seen between CT16 and CT20 in the SCN and between CT8 and CT12 in the heart.2 Publications

Gene expression databases

BgeeiENSMUSG00000020644 Expressed in 358 organ(s), highest expression level in adrenal gland
CleanExiMM_ID2
ExpressionAtlasiP41136 baseline and differential
GenevisibleiP41136 MM

Interactioni

Subunit structurei

Heterodimer with other HLH proteins. Interacts with NR0B2. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with GATA4, IFI204 and NKX2-5.By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200506, 16 interactors
DIPiDIP-167N
IntActiP41136, 18 interactors
MINTiP41136
STRINGi10090.ENSMUSP00000020974

Structurei

3D structure databases

ProteinModelPortaliP41136
SMRiP41136
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 75bHLHPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 83Interaction with IFI2041 PublicationAdd BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 115Nuclear export signal10

Domaini

The bHLH domain is essential for its repressor activity towards the CLOCK-ARNTL/BMAL1 heterodimer.

Phylogenomic databases

eggNOGiENOG410IVXR Eukaryota
ENOG4111PGK LUCA
GeneTreeiENSGT00390000000908
HOGENOMiHOG000234788
HOVERGENiHBG009009
InParanoidiP41136
KOiK17693
OMAiFPSEFMS
OrthoDBiEOG091G0VHR
PhylomeDBiP41136
TreeFamiTF326217

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR026052 DNA-bd_prot-inh
IPR036638 HLH_DNA-bd_sf
PANTHERiPTHR11723 PTHR11723, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P41136-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV
60 70 80 90 100
PSIPQNKKVT KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR
110 120 130
TPLTTLNTDI SILSLQASEF PSELMSNDSK VLCG
Length:134
Mass (Da):14,959
Last modified:May 30, 2000 - v3
Checksum:iA0D98D54F2686CC5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VMT3A0A1Y7VMT3_MOUSE
DNA-binding protein inhibitor ID-2
Id2
155Annotation score:

Sequence cautioni

The sequence AAA79771 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 8SPVR → RSGE in AAA79771 (PubMed:1922066).Curated4
Sequence conflicti102P → R in AAA79771 (PubMed:1922066).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69293 mRNA Translation: AAA79771.1 Different initiation.
AF077860 Genomic DNA Translation: AAD05214.1
BC006921 mRNA Translation: AAH06921.1
BC053699 mRNA Translation: AAH53699.1
CCDSiCCDS25846.1
RefSeqiNP_034626.1, NM_010496.3
UniGeneiMm.34871

Genome annotation databases

EnsembliENSMUST00000020974; ENSMUSP00000020974; ENSMUSG00000020644
ENSMUST00000221761; ENSMUSP00000152052; ENSMUSG00000020644
GeneIDi15902
KEGGimmu:15902
UCSCiuc007nfe.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69293 mRNA Translation: AAA79771.1 Different initiation.
AF077860 Genomic DNA Translation: AAD05214.1
BC006921 mRNA Translation: AAH06921.1
BC053699 mRNA Translation: AAH53699.1
CCDSiCCDS25846.1
RefSeqiNP_034626.1, NM_010496.3
UniGeneiMm.34871

3D structure databases

ProteinModelPortaliP41136
SMRiP41136
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200506, 16 interactors
DIPiDIP-167N
IntActiP41136, 18 interactors
MINTiP41136
STRINGi10090.ENSMUSP00000020974

PTM databases

iPTMnetiP41136
PhosphoSitePlusiP41136

Proteomic databases

PaxDbiP41136
PeptideAtlasiP41136
PRIDEiP41136

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020974; ENSMUSP00000020974; ENSMUSG00000020644
ENSMUST00000221761; ENSMUSP00000152052; ENSMUSG00000020644
GeneIDi15902
KEGGimmu:15902
UCSCiuc007nfe.2 mouse

Organism-specific databases

CTDi3398
MGIiMGI:96397 Id2

Phylogenomic databases

eggNOGiENOG410IVXR Eukaryota
ENOG4111PGK LUCA
GeneTreeiENSGT00390000000908
HOGENOMiHOG000234788
HOVERGENiHBG009009
InParanoidiP41136
KOiK17693
OMAiFPSEFMS
OrthoDBiEOG091G0VHR
PhylomeDBiP41136
TreeFamiTF326217

Miscellaneous databases

PROiPR:P41136
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020644 Expressed in 358 organ(s), highest expression level in adrenal gland
CleanExiMM_ID2
ExpressionAtlasiP41136 baseline and differential
GenevisibleiP41136 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR026052 DNA-bd_prot-inh
IPR036638 HLH_DNA-bd_sf
PANTHERiPTHR11723 PTHR11723, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiID2_MOUSE
AccessioniPrimary (citable) accession number: P41136
Secondary accession number(s): O88604
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: September 12, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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