UniProtKB - P41133 (ID3_MOUSE)
Protein
DNA-binding protein inhibitor ID-3
Gene
Id3
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.3 Publications
GO - Molecular functioni
- DNA-binding transcription factor inhibitor activity Source: UniProtKB
- leptomycin B binding Source: MGI
- protein dimerization activity Source: InterPro
- protein domain specific binding Source: MGI
- transcription factor binding Source: UniProtKB
GO - Biological processi
- cell differentiation Source: GO_Central
- cellular response to leptomycin B Source: MGI
- central nervous system development Source: UniProtKB
- circadian regulation of gene expression Source: GO_Central
- circadian rhythm Source: UniProtKB
- epithelial cell differentiation Source: MGI
- heart development Source: MGI
- metanephros development Source: UniProtKB
- muscle organ development Source: UniProtKB-KW
- negative regulation of DNA-binding transcription factor activity Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of myoblast differentiation Source: UniProtKB
- negative regulation of osteoblast differentiation Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron differentiation Source: Ensembl
- notochord development Source: UniProtKB
- odontogenesis Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of gene expression Source: UniProtKB
- regulation of cell cycle Source: MGI
- regulation of DNA replication Source: MGI
- response to wounding Source: Ensembl
Keywordsi
Molecular function | Developmental protein, Repressor |
Biological process | Biological rhythms, Myogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding protein inhibitor ID-3Alternative name(s): ID-like protein inhibitor HLH 462 Inhibitor of DNA binding 3 Inhibitor of differentiation 3 |
Gene namesi | Name:Id3 Synonyms:Hlh462, Id-3, Idb3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96398, Id3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127248 | 1 – 119 | DNA-binding protein inhibitor ID-3Add BLAST | 119 |
Post-translational modificationi
Polyubiquitinated; which is favored by Ifi204 and leads to proteasomal degradation.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
jPOSTi | P41133 |
PaxDbi | P41133 |
PRIDEi | P41133 |
PTM databases
iPTMneti | P41133 |
PhosphoSitePlusi | P41133 |
Expressioni
Tissue specificityi
Expressed by myoblasts (at protein level).1 Publication
Inductioni
By a variety of mitogenic agents in serum starved cells. Expressed in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and heart with peak levels between CT16 and CT20 in SCN and between CT8 and CT12 in the heart.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000007872, Expressed in tracheobronchial tree and 338 other tissues |
ExpressionAtlasi | P41133, baseline and differential |
Genevisiblei | P41133, MM |
Interactioni
Subunit structurei
Homodimer, and heterodimer with other HLH proteins.
Interacts with CLOCK and ARNTL/BMAL1 (By similarity).
Interacts with COPS5 and COPS7A.
Interacts with IFI204.
Interacts with GATA4 and NKX2-5.
Interacts with ANKRD2; both proteins cooperate in myoblast differentiation.
By similarity4 PublicationsBinary interactionsi
P41133
With | #Exp. | IntAct |
---|---|---|
Ankrd2 [Q9WV06] | 4 | EBI-309448,EBI-8854438 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
- protein domain specific binding Source: MGI
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 200507, 11 interactors |
IntActi | P41133, 4 interactors |
MINTi | P41133 |
STRINGi | 10090.ENSMUSP00000008016 |
Miscellaneous databases
RNActi | P41133, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 80 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 87 | Interaction with IFI204By similarityAdd BLAST | 53 |
Phylogenomic databases
eggNOGi | ENOG502S53I, Eukaryota |
GeneTreei | ENSGT00940000160504 |
HOGENOMi | CLU_116790_1_0_1 |
InParanoidi | P41133 |
OMAi | RNKHSCL |
OrthoDBi | 1624054at2759 |
PhylomeDBi | P41133 |
TreeFami | TF326217 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR026052, DNA-bd_prot-inh IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR11723, PTHR11723, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
P41133-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKALSPVRGC YEAVCCLSER SLAIARGRGK SPSTEEPLSL LDDMNHCYSR
60 70 80 90 100
LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ
110
TAELTPELVI SKDKRSFCH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M60523 mRNA Translation: AAA37818.1 |
CCDSi | CCDS18800.1 |
PIRi | A39114 |
RefSeqi | NP_032347.1, NM_008321.2 |
Genome annotation databases
Ensembli | ENSMUST00000008016; ENSMUSP00000008016; ENSMUSG00000007872 |
GeneIDi | 15903 |
KEGGi | mmu:15903 |
UCSCi | uc012dnf.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M60523 mRNA Translation: AAA37818.1 |
CCDSi | CCDS18800.1 |
PIRi | A39114 |
RefSeqi | NP_032347.1, NM_008321.2 |
3D structure databases
SMRi | P41133 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200507, 11 interactors |
IntActi | P41133, 4 interactors |
MINTi | P41133 |
STRINGi | 10090.ENSMUSP00000008016 |
PTM databases
iPTMneti | P41133 |
PhosphoSitePlusi | P41133 |
Proteomic databases
jPOSTi | P41133 |
PaxDbi | P41133 |
PRIDEi | P41133 |
Protocols and materials databases
Antibodypediai | 4480, 585 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000008016; ENSMUSP00000008016; ENSMUSG00000007872 |
GeneIDi | 15903 |
KEGGi | mmu:15903 |
UCSCi | uc012dnf.1, mouse |
Organism-specific databases
CTDi | 3399 |
MGIi | MGI:96398, Id3 |
Phylogenomic databases
eggNOGi | ENOG502S53I, Eukaryota |
GeneTreei | ENSGT00940000160504 |
HOGENOMi | CLU_116790_1_0_1 |
InParanoidi | P41133 |
OMAi | RNKHSCL |
OrthoDBi | 1624054at2759 |
PhylomeDBi | P41133 |
TreeFami | TF326217 |
Miscellaneous databases
BioGRID-ORCSi | 15903, 0 hits in 17 CRISPR screens |
ChiTaRSi | Id3, mouse |
PROi | PR:P41133 |
RNActi | P41133, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000007872, Expressed in tracheobronchial tree and 338 other tissues |
ExpressionAtlasi | P41133, baseline and differential |
Genevisiblei | P41133, MM |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR026052, DNA-bd_prot-inh IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR11723, PTHR11723, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ID3_MOUSE | |
Accessioni | P41133Primary (citable) accession number: P41133 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot