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Protein

Cyclin-F

Gene

CCNF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.1 Publication

Miscellaneous

Founding member of the F-box domain protein family, which obtained its name from cyclin-F.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP41002

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-F
Alternative name(s):
F-box only protein 1
Gene namesi
Name:CCNF
Synonyms:FBX1, FBXO1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000162063.12
HGNCiHGNC:1591 CCNF
MIMi600227 gene
neXtProtiNX_P41002

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35 – 36LP → AA: Impairs interaction with SKP1 and CUL1 and prevents degradation of CP110, leading to promote the formation of micronuclei. 1 Publication2
Mutagenesisi309M → A: Abolishes ability to interact with CP110; when associated with A-352. 1 Publication1
Mutagenesisi352L → A: Abolishes ability to interact with CP110; when associated with A-309. 1 Publication1

Organism-specific databases

DisGeNETi899
MalaCardsiCCNF
OpenTargetsiENSG00000162063
Orphaneti803 Amyotrophic lateral sclerosis
PharmGKBiPA26156

Polymorphism and mutation databases

BioMutaiCCNF
DMDMi20178283

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000804631 – 786Cyclin-FAdd BLAST786

Post-translational modificationi

Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence.
Phosphorylated just before cells enter into mitosis.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41002
PaxDbiP41002
PeptideAtlasiP41002
PRIDEiP41002
ProteomicsDBi55398

PTM databases

iPTMnetiP41002
PhosphoSitePlusiP41002

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Appears in S phase, peaks in G2 phase and disappears as cells enter into mitosis (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000162063 Expressed in 183 organ(s), highest expression level in female gonad
CleanExiHS_CCNF
ExpressionAtlasiP41002 baseline and differential
GenevisibleiP41002 HS

Organism-specific databases

HPAiHPA060596
HPA070495
HPA071600

Interactioni

Subunit structurei

Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this interaction leads to CCP110 ubiquitination and degradation via the proteasome pathway.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107339, 18 interactors
CORUMiP41002
DIPiDIP-44939N
IntActiP41002, 7 interactors
MINTiP41002
STRINGi9606.ENSP00000380256

Structurei

3D structure databases

ProteinModelPortaliP41002
SMRiP41002
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 76F-boxPROSITE-ProRule annotationAdd BLAST48
Domaini292 – 405Cyclin N-terminalAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni582 – 766PESTAdd BLAST185

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 28Nuclear localization signal 11 Publication9
Motifi568 – 574Nuclear localization signal 21 Publication7

Domaini

The nuclear localization signals mediate the localization to the nucleus and are required for CCNB1 localization to the nucleus.1 Publication

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiKOG0654 Eukaryota
COG5024 LUCA
GeneTreeiENSGT00810000125541
HOGENOMiHOG000285971
HOVERGENiHBG050838
InParanoidiP41002
KOiK10289
OMAiLHQKCFH
OrthoDBiEOG091G0AU2
PhylomeDBiP41002
TreeFamiTF101006

Family and domain databases

CDDicd00043 CYCLIN, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR028857 CCNF_metazoan
IPR039361 Cyclin
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR004367 Cyclin_C-dom
IPR006671 Cyclin_N
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR10177 PTHR10177, 1 hit
PTHR10177:SF252 PTHR10177:SF252, 1 hit
PfamiView protein in Pfam
PF02984 Cyclin_C, 1 hit
PF00134 Cyclin_N, 1 hit
PF00646 F-box, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01332 Cyclin_C, 1 hit
SM00256 FBOX, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS00292 CYCLINS, 1 hit
PS50181 FBOX, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P41002-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSGGVVHCR CAKCFCYPTK RRIRRRPRNL TILSLPEDVL FHILKWLSVE
60 70 80 90 100
DILAVRAVHS QLKDLVDNHA SVWACASFQE LWPSPGNLKL FERAAEKGNF
110 120 130 140 150
EAAVKLGIAY LYNEGLSVSD EARAEVNGLK ASRFFSLAER LNVGAAPFIW
160 170 180 190 200
LFIRPPWSVS GSCCKAVVHE SLRAECQLQR THKASILHCL GRVLSLFEDE
210 220 230 240 250
EKQQQAHDLF EEAAHQGCLT SSYLLWESDR RTDVSDPGRC LHSFRKLRDY
260 270 280 290 300
AAKGCWEAQL SLAKACANAN QLGLEVRASS EIVCQLFQAS QAVSKQQVFS
310 320 330 340 350
VQKGLNDTMR YILIDWLVEV ATMKDFTSLC LHLTVECVDR YLRRRLVPRY
360 370 380 390 400
RLQLLGIACM VICTRFISKE ILTIREAVWL TDNTYKYEDL VRMMGEIVSA
410 420 430 440 450
LEGKIRVPTV VDYKEVLLTL VPVELRTQHL CSFLCELSLL HTSLSAYAPA
460 470 480 490 500
RLAAAALLLA RLTHGQTQPW TTQLWDLTGF SYEDLIPCVL SLHKKCFHDD
510 520 530 540 550
APKDYRQVSL TAVKQRFEDK RYGEISQEEV LSYSQLCAAL GVTQDSPDPP
560 570 580 590 600
TFLSTGEIHA FLSSPSGRRT KRKRENSLQE DRGSFVTTPT AELSSQEETL
610 620 630 640 650
LGSFLDWSLD CCSGYEGDQE SEGEKEGDVT APSGILDVTV VYLNPEQHCC
660 670 680 690 700
QESSDEEACP EDKGPQDPQA LALDTQIPAT PGPKPLVRTS REPGKDVTTS
710 720 730 740 750
GYSSVSTASP TSSVDGGLGA LPQPTSVLSL DSDSHTQPCH HQARKSCLQC
760 770 780
RPPSPPESSV PQQQVKRINL CIHSEEEDMN LGLVRL
Length:786
Mass (Da):87,640
Last modified:April 16, 2002 - v2
Checksum:iADB2FE41BC708898
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BUD3H3BUD3_HUMAN
Cyclin-F
CCNF
186Annotation score:
H0Y2P7H0Y2P7_HUMAN
Cyclin-F
CCNF
59Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252A → R in CAA85308 (PubMed:7896286).Curated1
Sequence conflicti324K → N in CAA85308 (PubMed:7896286).Curated1
Sequence conflicti434L → H in BAG36170 (PubMed:14702039).Curated1
Sequence conflicti600L → V in CAA85308 (PubMed:7896286).Curated1
Sequence conflicti662D → A in CAA85308 (PubMed:7896286).Curated1
Sequence conflicti710P → S in AAB60342 (PubMed:7813445).Curated1
Sequence conflicti731D → H in CAA85308 (PubMed:7896286).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17105 mRNA Translation: AAB60342.1
Z36714 mRNA Translation: CAA85308.1
AK313371 mRNA Translation: BAG36170.1
AC106820 Genomic DNA No translation available.
BC012349 mRNA Translation: AAH12349.1
CCDSiCCDS10467.1
PIRiA55501
RefSeqiNP_001752.2, NM_001761.2
UniGeneiHs.1973

Genome annotation databases

EnsembliENST00000397066; ENSP00000380256; ENSG00000162063
GeneIDi899
KEGGihsa:899
UCSCiuc002cqd.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17105 mRNA Translation: AAB60342.1
Z36714 mRNA Translation: CAA85308.1
AK313371 mRNA Translation: BAG36170.1
AC106820 Genomic DNA No translation available.
BC012349 mRNA Translation: AAH12349.1
CCDSiCCDS10467.1
PIRiA55501
RefSeqiNP_001752.2, NM_001761.2
UniGeneiHs.1973

3D structure databases

ProteinModelPortaliP41002
SMRiP41002
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107339, 18 interactors
CORUMiP41002
DIPiDIP-44939N
IntActiP41002, 7 interactors
MINTiP41002
STRINGi9606.ENSP00000380256

PTM databases

iPTMnetiP41002
PhosphoSitePlusiP41002

Polymorphism and mutation databases

BioMutaiCCNF
DMDMi20178283

Proteomic databases

MaxQBiP41002
PaxDbiP41002
PeptideAtlasiP41002
PRIDEiP41002
ProteomicsDBi55398

Protocols and materials databases

DNASUi899
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397066; ENSP00000380256; ENSG00000162063
GeneIDi899
KEGGihsa:899
UCSCiuc002cqd.2 human

Organism-specific databases

CTDi899
DisGeNETi899
EuPathDBiHostDB:ENSG00000162063.12
GeneCardsiCCNF
HGNCiHGNC:1591 CCNF
HPAiHPA060596
HPA070495
HPA071600
MalaCardsiCCNF
MIMi600227 gene
neXtProtiNX_P41002
OpenTargetsiENSG00000162063
Orphaneti803 Amyotrophic lateral sclerosis
PharmGKBiPA26156
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0654 Eukaryota
COG5024 LUCA
GeneTreeiENSGT00810000125541
HOGENOMiHOG000285971
HOVERGENiHBG050838
InParanoidiP41002
KOiK10289
OMAiLHQKCFH
OrthoDBiEOG091G0AU2
PhylomeDBiP41002
TreeFamiTF101006

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP41002

Miscellaneous databases

ChiTaRSiCCNF human
GeneWikiiCCNF
GenomeRNAii899
PROiPR:P41002
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162063 Expressed in 183 organ(s), highest expression level in female gonad
CleanExiHS_CCNF
ExpressionAtlasiP41002 baseline and differential
GenevisibleiP41002 HS

Family and domain databases

CDDicd00043 CYCLIN, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR028857 CCNF_metazoan
IPR039361 Cyclin
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR004367 Cyclin_C-dom
IPR006671 Cyclin_N
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR10177 PTHR10177, 1 hit
PTHR10177:SF252 PTHR10177:SF252, 1 hit
PfamiView protein in Pfam
PF02984 Cyclin_C, 1 hit
PF00134 Cyclin_N, 1 hit
PF00646 F-box, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01332 Cyclin_C, 1 hit
SM00256 FBOX, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS00292 CYCLINS, 1 hit
PS50181 FBOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCCNF_HUMAN
AccessioniPrimary (citable) accession number: P41002
Secondary accession number(s): B2R8H3, Q96EG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 16, 2002
Last modified: November 7, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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