UniProtKB - P40989 (FKS2_YEAST)
Protein
1,3-beta-glucan synthase component GSC2
Gene
GSC2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Required for spore wall assembly. Negative regulation of activity by SMK1 is important for spore wall deposition. Activity is positively regulated by RHO1.2 Publications
Miscellaneous
Deletion leads to caspofungin resistance.
Catalytic activityi
- EC:2.4.1.342 Publications
GO - Molecular functioni
- 1,3-beta-D-glucan synthase activity Source: SGD
- glucosyltransferase activity Source: GO_Central
GO - Biological processi
- (1->3)-beta-D-glucan biosynthetic process Source: SGD
- ascospore wall (1->3)-beta-D-glucan biosynthetic process Source: GO_Central
- ascospore wall assembly Source: SGD
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cell shape, Cell wall biogenesis/degradation, Sporulation |
Protein family/group databases
CAZyi | GT48, Glycosyltransferase Family 48 |
Names & Taxonomyi
Protein namesi | Recommended name: 1,3-beta-glucan synthase component GSC2 (EC:2.4.1.34)Alternative name(s): 1,3-beta-D-glucan-UDP glucosyltransferase FK506 sensitivity protein 2 Glucan synthase of cerevisiae protein 2 |
Gene namesi | Name:GSC2 Synonyms:FKS2, GLS2 Ordered Locus Names:YGR032W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGR032W |
SGDi | S000003264, GSC2 |
Subcellular locationi
Other locations
- Membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- 1,3-beta-D-glucan synthase complex Source: SGD
- plasma membrane Source: GO_Central
Other locations
- cell periphery Source: SGD
- cellular bud neck Source: SGD
- integral component of membrane Source: UniProtKB-KW
- prospore membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 473 | ExtracellularSequence analysisAdd BLAST | 473 | |
Transmembranei | 474 – 494 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 495 – 511 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 512 – 532 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 533 – 550 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 551 – 571 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 572 – 582 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 583 – 603 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 604 – 1579 | ExtracellularSequence analysisAdd BLAST | 976 | |
Transmembranei | 1580 – 1600 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1601 – 1620 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 1621 – 1641 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1642 – 1758 | ExtracellularSequence analysisAdd BLAST | 117 | |
Transmembranei | 1759 – 1779 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1780 – 1821 | CytoplasmicSequence analysisAdd BLAST | 42 | |
Transmembranei | 1822 – 1842 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1843 – 1895 | ExtracellularSequence analysisAdd BLAST | 53 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121726 | 1 – 1895 | 1,3-beta-glucan synthase component GSC2Add BLAST | 1895 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 278 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 288 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 291 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 405 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 929 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 934 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 1558 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 1566 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P40989 |
PaxDbi | P40989 |
PRIDEi | P40989 |
TopDownProteomicsi | P40989 |
PTM databases
iPTMneti | P40989 |
Expressioni
Inductioni
Under starvation and during sporulation. Also by pheromones and calcium in a calcineurin-dependent manner.1 Publication
Interactioni
Subunit structurei
Component of the 1,3-beta-glucan synthase (GS), composed of two alternate catalytic subunits FKS1 or GSC2, and a regulatory subunit RHO1.
Interacts with SMK1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33277, 89 interactors |
ComplexPortali | CPX-1813, 1,3-beta-D-glucan synthase complex |
DIPi | DIP-2511N |
IntActi | P40989, 6 interactors |
MINTi | P40989 |
STRINGi | 4932.YGR032W |
Chemistry databases
BindingDBi | P40989 |
Miscellaneous databases
RNActi | P40989, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 48 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0916, Eukaryota |
GeneTreei | ENSGT00940000176776 |
HOGENOMi | CLU_000844_0_1_1 |
InParanoidi | P40989 |
OMAi | AEWAYVP |
Family and domain databases
InterProi | View protein in InterPro IPR026899, FKS1-like_dom1 IPR003440, Glyco_trans_48 |
Pfami | View protein in Pfam PF14288, FKS1_dom1, 1 hit PF02364, Glucan_synthase, 1 hit |
SMARTi | View protein in SMART SM01205, FKS1_dom1, 1 hit |
i Sequence
Sequence statusi: Complete.
P40989-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYNDPNLNG QYYSNGDGTG DGNYPTYQVT QDQSAYDEYG QPIYTQNQLD
60 70 80 90 100
DGYYDPNEQY VDGTQFPQGQ DPSQDQGPYN NDASYYNQPP NMMNPSSQDG
110 120 130 140 150
ENFSDFSSYG PPSGTYPNDQ YTPSQMSYPD QDGSSGASTP YGNGVVNGNG
160 170 180 190 200
QYYDPNAIEM ALPNDPYPAW TADPQSPLPI EQIEDIFIDL TNKFGFQRDS
210 220 230 240 250
MRNMFDHFMT LLDSRSSRMS PEQALLSLHA DYIGGDTANY KKWYFAAQLD
260 270 280 290 300
MDDEIGFRNM KLGKLSRKAR KAKKKNKKAM QEASPEDTEE TLNQIEGDNS
310 320 330 340 350
LEAADFRWKS KMNQLSPFEM VRQIALFLLC WGEANQVRFT PECLCFIYKC
360 370 380 390 400
ASDYLDSAQC QQRPDPLPEG DFLNRVITPL YRFIRSQVYE IVDGRYVKSE
410 420 430 440 450
KDHNKVIGYD DVNQLFWYPE GIAKIVMEDG TRLIDLPAEE RYLKLGEIPW
460 470 480 490 500
DDVFFKTYKE TRSWLHLVTN FNRIWIMHIS VYWMYCAYNA PTFYTHNYQQ
510 520 530 540 550
LVDNQPLAAY KWATAALGGT VASLIQVAAT LCEWSFVPRK WAGAQHLSRR
560 570 580 590 600
FWFLCVIMGI NLGPVIFVFA YDKDTVYSTA AHVVGAVMFF VAVATLVFFS
610 620 630 640 650
VMPLGGLFTS YMKKSTRSYV ASQTFTASFA PLHGLDRWMS YLVWVTVFAA
660 670 680 690 700
KYAESYFFLI LSLRDPIRIL STTSMRCTGE YWWGNKICKV QPKIVLGLMI
710 720 730 740 750
ATDFILFFLD TYLWYIVVNT VFSVGKSFYL GISILTPWRN IFTRLPKRIY
760 770 780 790 800
SKILATTDME IKYKPKVLIS QIWNAIIISM YREHLLAIDH VQKLLYHQVP
810 820 830 840 850
SEIEGKRTLR APTFFVSQDD NNFETEFFPR DSEAERRISF FAQSLSTPIP
860 870 880 890 900
EPLPVDNMPT FTVLTPHYAE RILLSLREII REDDQFSRVT LLEYLKQLHP
910 920 930 940 950
VEWDCFVKDT KILAEETAAY ENNEDEPEKE DALKSQIDDL PFYCIGFKSA
960 970 980 990 1000
APEYTLRTRI WASLRSQTLY RTISGFMNYS RAIKLLYRVE NPEIVQMFGG
1010 1020 1030 1040 1050
NADGLERELE KMARRKFKFL VSMQRLAKFK PHELENAEFL LRAYPDLQIA
1060 1070 1080 1090 1100
YLDEEPPLNE GEEPRIYSAL IDGHCEILEN GRRRPKFRVQ LSGNPILGDG
1110 1120 1130 1140 1150
KSDNQNHALI FYRGEYIQLI DANQDNYLEE CLKIRSVLAE FEELGIEQIH
1160 1170 1180 1190 1200
PYTPGLKYED QSTNHPVAIV GAREYIFSEN SGVLGDVAAG KEQTFGTLFA
1210 1220 1230 1240 1250
RTLAQIGGKL HYGHPDFINA TFMTTRGGVS KAQKGLHLNE DIYAGMNAVL
1260 1270 1280 1290 1300
RGGRIKHCEY YQCGKGRDLG FGTILNFTTK IGAGMGEQML SREYYYLGTQ
1310 1320 1330 1340 1350
LPIDRFLTFY YAHPGFHLNN LFIQLSLQMF MLTLVNLHAL AHESILCVYD
1360 1370 1380 1390 1400
RDKPITDVLY PIGCYNFHPA IDWVRRYTLS IFIVFWIAFV PIVVQELIER
1410 1420 1430 1440 1450
GLWKATQRFF RHILSLSPMF EVFAGQIYSS ALLSDIAVGG ARYISTGRGF
1460 1470 1480 1490 1500
ATSRIPFSIL YSRFAGSAIY MGSRSMLMLL FGTVAHWQAP LLWFWASLSA
1510 1520 1530 1540 1550
LIFAPFIFNP HQFAWEDFFL DYRDYIRWLS RGNNKYHRNS WIGYVRMSRS
1560 1570 1580 1590 1600
RVTGFKRKLV GDESEKSAGD ASRAHRTNLI MAEIIPCAIY AAGCFIAFTF
1610 1620 1630 1640 1650
INAQTGVKTT DEDRVNSTLR IIICTLAPIV IDIGVLFFCM GLSCCSGPLL
1660 1670 1680 1690 1700
GMCCKKTGSV MAGIAHGIAV VVHIVFFIVM WVLEGFSFVR MLIGVVTCIQ
1710 1720 1730 1740 1750
CQRLIFHCMT VLLLTREFKN DHANTAFWTG KWYSTGLGYM AWTQPTRELT
1760 1770 1780 1790 1800
AKVIELSEFA ADFVLGHVIL IFQLPVICIP KIDKFHSIML FWLKPSRQIR
1810 1820 1830 1840 1850
PPIYSLKQAR LRKRMVRRYC SLYFLVLIIF AGCIVGPAVA SAHVPKDLGS
1860 1870 1880 1890
GLTGTFHNLV QPRNVSNNDT GSQMSTYKSH YYTHTPSLKT WSTIK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 137 | A → T in BAA07707 (PubMed:7649185).Curated | 1 | |
Sequence conflicti | 261 | K → E in BAA07707 (PubMed:7649185).Curated | 1 | |
Sequence conflicti | 275 | K → R in BAA07707 (PubMed:7649185).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 1059 | N → S in strain: SK1. 1 Publication | 1 | |
Natural varianti | 1853 – 1854 | TG → KD in strain: SK1. | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D42127 Genomic DNA Translation: BAA07707.1 U16783 Genomic DNA Translation: AAA85676.1 DQ115390 Genomic DNA Translation: AAZ22447.1 Z72817 Genomic DNA Translation: CAA97020.1 BK006941 Genomic DNA Translation: DAA08129.1 |
PIRi | S50240 |
RefSeqi | NP_011546.3, NM_001181161.3 |
Genome annotation databases
EnsemblFungii | YGR032W_mRNA; YGR032W; YGR032W |
GeneIDi | 852920 |
KEGGi | sce:YGR032W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D42127 Genomic DNA Translation: BAA07707.1 U16783 Genomic DNA Translation: AAA85676.1 DQ115390 Genomic DNA Translation: AAZ22447.1 Z72817 Genomic DNA Translation: CAA97020.1 BK006941 Genomic DNA Translation: DAA08129.1 |
PIRi | S50240 |
RefSeqi | NP_011546.3, NM_001181161.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33277, 89 interactors |
ComplexPortali | CPX-1813, 1,3-beta-D-glucan synthase complex |
DIPi | DIP-2511N |
IntActi | P40989, 6 interactors |
MINTi | P40989 |
STRINGi | 4932.YGR032W |
Chemistry databases
BindingDBi | P40989 |
ChEMBLi | CHEMBL3134 |
Protein family/group databases
CAZyi | GT48, Glycosyltransferase Family 48 |
PTM databases
iPTMneti | P40989 |
Proteomic databases
MaxQBi | P40989 |
PaxDbi | P40989 |
PRIDEi | P40989 |
TopDownProteomicsi | P40989 |
Genome annotation databases
EnsemblFungii | YGR032W_mRNA; YGR032W; YGR032W |
GeneIDi | 852920 |
KEGGi | sce:YGR032W |
Organism-specific databases
EuPathDBi | FungiDB:YGR032W |
SGDi | S000003264, GSC2 |
Phylogenomic databases
eggNOGi | KOG0916, Eukaryota |
GeneTreei | ENSGT00940000176776 |
HOGENOMi | CLU_000844_0_1_1 |
InParanoidi | P40989 |
OMAi | AEWAYVP |
Miscellaneous databases
PROi | PR:P40989 |
RNActi | P40989, protein |
Family and domain databases
InterProi | View protein in InterPro IPR026899, FKS1-like_dom1 IPR003440, Glyco_trans_48 |
Pfami | View protein in Pfam PF14288, FKS1_dom1, 1 hit PF02364, Glucan_synthase, 1 hit |
SMARTi | View protein in SMART SM01205, FKS1_dom1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FKS2_YEAST | |
Accessioni | P40989Primary (citable) accession number: P40989 Secondary accession number(s): D6VUG8, Q45U52 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names