GSC2

Functionⁱ

Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Required for spore wall assembly. Negative regulation of activity by SMK1 is important for spore wall deposition. Activity is positively regulated by RHO1.2 Publications

Miscellaneous

Deletion leads to caspofungin resistance.

Catalytic activityⁱ

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).2 Publications

GO - Molecular functionⁱ

1,3-beta-D-glucan synthase activity
Source: SGD
Inferred from direct assayⁱ
- 7565718
- 7649185

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

(1->3)-beta-D-glucan biosynthetic process
Source: SGD
Inferred from direct assayⁱ
- 7565718
ascospore wall assembly
Source: SGD
Inferred from mutant phenotypeⁱ
- 16116083
regulation of cell shape Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Cell shape, Cell wall biogenesis/degradation, Sporulation

Enzyme and pathway databases

BioCycⁱ	YEAST:YGR032W-MONOMER

BioCycⁱ

YEAST:YGR032W-MONOMER

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT48 Glycosyltransferase Family 48

CAZyⁱ

GT48 Glycosyltransferase Family 48

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 1,3-beta-glucan synthase component GSC2 (EC:2.4.1.34) Alternative name(s): 1,3-beta-D-glucan-UDP glucosyltransferase FK506 sensitivity protein 2 Glucan synthase of cerevisiae protein 2
Gene namesⁱ	Name:GSC2 Synonyms:FKS2, GLS2 Ordered Locus Names:YGR032W
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome VII

Organism-specific databases

EuPathDBⁱ	FungiDB:YGR032W
Saccharomyces Genome Database More...SGDⁱ	S000003264 GSC2

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 473	ExtracellularSequence analysisAdd BLAST	473
Transmembraneⁱ	474 – 494	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	495 – 511	CytoplasmicSequence analysisAdd BLAST	17
Transmembraneⁱ	512 – 532	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	533 – 550	ExtracellularSequence analysisAdd BLAST	18
Transmembraneⁱ	551 – 571	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	572 – 582	CytoplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	583 – 603	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	604 – 1579	ExtracellularSequence analysisAdd BLAST	976
Transmembraneⁱ	1580 – 1600	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	1601 – 1620	CytoplasmicSequence analysisAdd BLAST	20
Transmembraneⁱ	1621 – 1641	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	1642 – 1758	ExtracellularSequence analysisAdd BLAST	117
Transmembraneⁱ	1759 – 1779	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	1780 – 1821	CytoplasmicSequence analysisAdd BLAST	42
Transmembraneⁱ	1822 – 1842	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	1843 – 1895	ExtracellularSequence analysisAdd BLAST	53

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL3134

ChEMBLⁱ

CHEMBL3134

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000121726	1 – 1895	1,3-beta-glucan synthase component GSC2Add BLAST		1895

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	278	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	288	PhosphothreonineBy similarity	1
Modified residueⁱ	291	PhosphothreonineBy similarity	1
Cross-linkⁱ	405	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linkⁱ	929	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linkⁱ	934	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linkⁱ	1558	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linkⁱ	1566	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P40989
PaxDbⁱ	P40989
PRIDEⁱ	P40989
TopDownProteomicsⁱ	P40989

PTM databases

iPTMnetⁱ	P40989

iPTMnetⁱ

P40989

Expressionⁱ

Inductionⁱ

Under starvation and during sporulation. Also by pheromones and calcium in a calcineurin-dependent manner.1 Publication

Interactionⁱ

Subunit structureⁱ

Component of the 1,3-beta-glucan synthase (GS), composed of two alternate catalytic subunits FKS1 or GSC2, and a regulatory subunit RHO1. Interacts with SMK1.1 Publication

Protein-protein interaction databases

BioGridⁱ	33277, 88 interactors
ComplexPortalⁱ	CPX-1813 1,3-beta-D-glucan synthase complex
Database of interacting proteins More...DIPⁱ	DIP-2511N
IntActⁱ	P40989, 6 interactors
Molecular INTeraction database More...MINTⁱ	P40989
STRINGⁱ	4932.YGR032W

Chemistry databases

BindingDBⁱ	P40989

BindingDBⁱ

P40989

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P40989
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyltransferase 48 family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004828
HOGENOMⁱ	HOG000216604
InParanoidⁱ	P40989
KEGG Orthology (KO) More...KOⁱ	K00706
OMAⁱ	EFLWFLE
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C025F

Family and domain databases

InterProⁱ	View protein in InterPro IPR026899 FKS1-like_dom1 IPR003440 Glyco_trans_48
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF14288 FKS1_dom1, 1 hit PF02364 Glucan_synthase, 1 hit
SMARTⁱ	View protein in SMART SM01205 FKS1_dom1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P40989-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSYNDPNLNG QYYSNGDGTG DGNYPTYQVT QDQSAYDEYG QPIYTQNQLD 
        60         70         80         90        100
DGYYDPNEQY VDGTQFPQGQ DPSQDQGPYN NDASYYNQPP NMMNPSSQDG 
       110        120        130        140        150
ENFSDFSSYG PPSGTYPNDQ YTPSQMSYPD QDGSSGASTP YGNGVVNGNG 
       160        170        180        190        200
QYYDPNAIEM ALPNDPYPAW TADPQSPLPI EQIEDIFIDL TNKFGFQRDS 
       210        220        230        240        250
MRNMFDHFMT LLDSRSSRMS PEQALLSLHA DYIGGDTANY KKWYFAAQLD 
       260        270        280        290        300
MDDEIGFRNM KLGKLSRKAR KAKKKNKKAM QEASPEDTEE TLNQIEGDNS 
       310        320        330        340        350
LEAADFRWKS KMNQLSPFEM VRQIALFLLC WGEANQVRFT PECLCFIYKC 
       360        370        380        390        400
ASDYLDSAQC QQRPDPLPEG DFLNRVITPL YRFIRSQVYE IVDGRYVKSE 
       410        420        430        440        450
KDHNKVIGYD DVNQLFWYPE GIAKIVMEDG TRLIDLPAEE RYLKLGEIPW 
       460        470        480        490        500
DDVFFKTYKE TRSWLHLVTN FNRIWIMHIS VYWMYCAYNA PTFYTHNYQQ 
       510        520        530        540        550
LVDNQPLAAY KWATAALGGT VASLIQVAAT LCEWSFVPRK WAGAQHLSRR 
       560        570        580        590        600
FWFLCVIMGI NLGPVIFVFA YDKDTVYSTA AHVVGAVMFF VAVATLVFFS 
       610        620        630        640        650
VMPLGGLFTS YMKKSTRSYV ASQTFTASFA PLHGLDRWMS YLVWVTVFAA 
       660        670        680        690        700
KYAESYFFLI LSLRDPIRIL STTSMRCTGE YWWGNKICKV QPKIVLGLMI 
       710        720        730        740        750
ATDFILFFLD TYLWYIVVNT VFSVGKSFYL GISILTPWRN IFTRLPKRIY 
       760        770        780        790        800
SKILATTDME IKYKPKVLIS QIWNAIIISM YREHLLAIDH VQKLLYHQVP 
       810        820        830        840        850
SEIEGKRTLR APTFFVSQDD NNFETEFFPR DSEAERRISF FAQSLSTPIP 
       860        870        880        890        900
EPLPVDNMPT FTVLTPHYAE RILLSLREII REDDQFSRVT LLEYLKQLHP 
       910        920        930        940        950
VEWDCFVKDT KILAEETAAY ENNEDEPEKE DALKSQIDDL PFYCIGFKSA 
       960        970        980        990       1000
APEYTLRTRI WASLRSQTLY RTISGFMNYS RAIKLLYRVE NPEIVQMFGG 
      1010       1020       1030       1040       1050
NADGLERELE KMARRKFKFL VSMQRLAKFK PHELENAEFL LRAYPDLQIA 
      1060       1070       1080       1090       1100
YLDEEPPLNE GEEPRIYSAL IDGHCEILEN GRRRPKFRVQ LSGNPILGDG 
      1110       1120       1130       1140       1150
KSDNQNHALI FYRGEYIQLI DANQDNYLEE CLKIRSVLAE FEELGIEQIH 
      1160       1170       1180       1190       1200
PYTPGLKYED QSTNHPVAIV GAREYIFSEN SGVLGDVAAG KEQTFGTLFA 
      1210       1220       1230       1240       1250
RTLAQIGGKL HYGHPDFINA TFMTTRGGVS KAQKGLHLNE DIYAGMNAVL 
      1260       1270       1280       1290       1300
RGGRIKHCEY YQCGKGRDLG FGTILNFTTK IGAGMGEQML SREYYYLGTQ 
      1310       1320       1330       1340       1350
LPIDRFLTFY YAHPGFHLNN LFIQLSLQMF MLTLVNLHAL AHESILCVYD 
      1360       1370       1380       1390       1400
RDKPITDVLY PIGCYNFHPA IDWVRRYTLS IFIVFWIAFV PIVVQELIER 
      1410       1420       1430       1440       1450
GLWKATQRFF RHILSLSPMF EVFAGQIYSS ALLSDIAVGG ARYISTGRGF 
      1460       1470       1480       1490       1500
ATSRIPFSIL YSRFAGSAIY MGSRSMLMLL FGTVAHWQAP LLWFWASLSA 
      1510       1520       1530       1540       1550
LIFAPFIFNP HQFAWEDFFL DYRDYIRWLS RGNNKYHRNS WIGYVRMSRS 
      1560       1570       1580       1590       1600
RVTGFKRKLV GDESEKSAGD ASRAHRTNLI MAEIIPCAIY AAGCFIAFTF 
      1610       1620       1630       1640       1650
INAQTGVKTT DEDRVNSTLR IIICTLAPIV IDIGVLFFCM GLSCCSGPLL 
      1660       1670       1680       1690       1700
GMCCKKTGSV MAGIAHGIAV VVHIVFFIVM WVLEGFSFVR MLIGVVTCIQ 
      1710       1720       1730       1740       1750
CQRLIFHCMT VLLLTREFKN DHANTAFWTG KWYSTGLGYM AWTQPTRELT 
      1760       1770       1780       1790       1800
AKVIELSEFA ADFVLGHVIL IFQLPVICIP KIDKFHSIML FWLKPSRQIR 
      1810       1820       1830       1840       1850
PPIYSLKQAR LRKRMVRRYC SLYFLVLIIF AGCIVGPAVA SAHVPKDLGS 
      1860       1870       1880       1890 
GLTGTFHNLV QPRNVSNNDT GSQMSTYKSH YYTHTPSLKT WSTIK

Length:1,895

Mass (Da):216,990

Last modified:October 1, 1996 - v2

Checksum:ⁱ9DDE4D8C19BC24DA

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	137	A → T in BAA07707 (PubMed:7649185).Curated	1
Sequence conflictⁱ	261	K → E in BAA07707 (PubMed:7649185).Curated	1
Sequence conflictⁱ	275	K → R in BAA07707 (PubMed:7649185).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	1059	N → S in strain: SK1. 1 Publication		1
Natural variantⁱ	1853 – 1854	TG → KD in strain: SK1.		2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D42127 Genomic DNA Translation: BAA07707.1 U16783 Genomic DNA Translation: AAA85676.1 DQ115390 Genomic DNA Translation: AAZ22447.1 Z72817 Genomic DNA Translation: CAA97020.1 BK006941 Genomic DNA Translation: DAA08129.1
PIRⁱ	S50240
NCBI Reference Sequences More...RefSeqⁱ	NP_011546.3, NM_001181161.3

Genome annotation databases

EnsemblFungiⁱ	YGR032W; YGR032W; YGR032W
GeneIDⁱ	852920
KEGGⁱ	sce:YGR032W

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P40989	Gsc2p		SACAR		1899	UniRef90_P40989
GSC2-like protein		SACEU		1895
1,3-beta-D-glucan synthase		YEAS1		1895
GSC2p Catalytic subunit of 1,3-beta-glucan synthase		Saccharomyces sp. 'boulardii'		1895
YGR032Wp-like protein		YEAS6		1895
+7

Protein	Similar proteins		Species	Score	Length	Source
P40989	1,3-beta-glucan synthase component FKS1		YEAST		1876	UniRef50_P38631
1,3-beta-glucan synthase component FKS1		ASPNC		1897
1,3-beta-glucan synthase component bgs4		SCHPO		1955
1,3-beta-glucan synthase component FKS1		CRYNH		1799
Fks1p		YEASC		1876
+841

Entry informationⁱ

Entry nameⁱ	FKS2_YEAST
Accessionⁱ	P40989Primary (citable) accession number: P40989 Secondary accession number(s): D6VUG8, Q45U52
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
	Last sequence update:	October 1, 1996
	Last modified:	September 12, 2018
	This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P40989 (FKS2_YEAST)

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1,3-beta-glucan synthase component GSC2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ