UniProtKB - P40985 (HUL4_YEAST)
Protein
Probable E3 ubiquitin-protein ligase HUL4
Gene
HUL4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 860 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- protein ubiquitination Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Probable E3 ubiquitin-protein ligase HUL4 (EC:2.3.2.26)Alternative name(s): HECT ubiquitin ligase 4 HECT-type E3 ubiquitin transferase HUL4 |
Gene namesi | Name:HUL4 Ordered Locus Names:YJR036C ORF Names:J1608 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YJR036C |
SGDi | S000003797, HUL4 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- TRAMP complex Source: SGD
Other locations
- prospore membrane Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084094 | 1 – 892 | Probable E3 ubiquitin-protein ligase HUL4Add BLAST | 892 |
Proteomic databases
PaxDbi | P40985 |
PRIDEi | P40985 |
Interactioni
Subunit structurei
Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33789, 53 interactors |
ComplexPortali | CPX-1678, TRAMP complex variant 1 CPX-1679, TRAMP complex variant 2 |
IntActi | P40985, 1 interactor |
MINTi | P40985 |
STRINGi | 4932.YJR036C |
Miscellaneous databases
RNActi | P40985, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 792 – 892 | HECTPROSITE-ProRule annotationAdd BLAST | 101 |
Sequence similaritiesi
Belongs to the HUL4 family.Curated
Phylogenomic databases
eggNOGi | KOG0941, Eukaryota |
GeneTreei | ENSGT00940000163989 |
HOGENOMi | CLU_002173_5_1_1 |
InParanoidi | P40985 |
OMAi | YGNDWHI |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
InterProi | View protein in InterPro IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase |
Pfami | View protein in Pfam PF00632, HECT, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit |
SUPFAMi | SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit |
i Sequence
Sequence statusi: Complete.
P40985-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS
60 70 80 90 100
VKDGSCSNSK SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF
110 120 130 140 150
RCSACQVTVI VKEPEINSNL ESSTHISCTL EGLQMVVRRC HDDLQRLKKT
160 170 180 190 200
GILDKERKGL IFQPVITYLL DRFHDVSILN RSFLVHDGGK NIKMLNYEVL
210 220 230 240 250
QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI LQYRWLLIIL
260 270 280 290 300
NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL
310 320 330 340 350
IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM
360 370 380 390 400
RSRLRRHHTT GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED
410 420 430 440 450
WHIHSAAKLT FIYYVANTRR NGRGALSIQS FYNITLDFID YKQDFDHWRG
460 470 480 490 500
VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF ILSLGIKISI MEYEIRRIME
510 520 530 540 550
HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE HQGDLLKSLR
560 570 580 590 600
IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP
610 620 630 640 650
NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS
660 670 680 690 700
FEDYSELFPE TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS
710 720 730 740 750
SKEYVTVELC ENGRNVPITQ SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF
760 770 780 790 800
KRVFAECNSI KLFNSEELER LVCGDEEQTK FDFKSLRSVT KYVGGFSDDS
810 820 830 840 850
RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP FKISLLGSHD
860 870 880 890
SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 362 | H → Q in CAA57291 (PubMed:7957102).Curated | 1 | |
Sequence conflicti | 410 | T → M in CAA57291 (PubMed:7957102).Curated | 1 | |
Sequence conflicti | 418 | T → I in CAA57291 (PubMed:7957102).Curated | 1 | |
Sequence conflicti | 514 – 519 | GKSVDV → RQIRRR in CAA57291 (PubMed:7957102).Curated | 6 | |
Sequence conflicti | 589 – 607 | ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291 (PubMed:7957102).CuratedAdd BLAST | 19 | |
Sequence conflicti | 705 | V → A in CAA57291 (PubMed:7957102).Curated | 1 | |
Sequence conflicti | 723 – 724 | KH → ND in CAA57291 (PubMed:7957102).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36344 Genomic DNA Translation: AAA88738.1 Z49536 Genomic DNA Translation: CAA89563.1 X81635 Genomic DNA Translation: CAA57291.1 BK006943 Genomic DNA Translation: DAA08825.1 |
PIRi | S57055 |
RefSeqi | NP_012570.3, NM_001181694.3 |
Genome annotation databases
EnsemblFungii | YJR036C_mRNA; YJR036C; YJR036C |
GeneIDi | 853494 |
KEGGi | sce:YJR036C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36344 Genomic DNA Translation: AAA88738.1 Z49536 Genomic DNA Translation: CAA89563.1 X81635 Genomic DNA Translation: CAA57291.1 BK006943 Genomic DNA Translation: DAA08825.1 |
PIRi | S57055 |
RefSeqi | NP_012570.3, NM_001181694.3 |
3D structure databases
SMRi | P40985 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33789, 53 interactors |
ComplexPortali | CPX-1678, TRAMP complex variant 1 CPX-1679, TRAMP complex variant 2 |
IntActi | P40985, 1 interactor |
MINTi | P40985 |
STRINGi | 4932.YJR036C |
Proteomic databases
PaxDbi | P40985 |
PRIDEi | P40985 |
Genome annotation databases
EnsemblFungii | YJR036C_mRNA; YJR036C; YJR036C |
GeneIDi | 853494 |
KEGGi | sce:YJR036C |
Organism-specific databases
EuPathDBi | FungiDB:YJR036C |
SGDi | S000003797, HUL4 |
Phylogenomic databases
eggNOGi | KOG0941, Eukaryota |
GeneTreei | ENSGT00940000163989 |
HOGENOMi | CLU_002173_5_1_1 |
InParanoidi | P40985 |
OMAi | YGNDWHI |
Miscellaneous databases
PROi | PR:P40985 |
RNActi | P40985, protein |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
InterProi | View protein in InterPro IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase |
Pfami | View protein in Pfam PF00632, HECT, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit |
SUPFAMi | SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HUL4_YEAST | |
Accessioni | P40985Primary (citable) accession number: P40985 Secondary accession number(s): D6VWK9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names