UniProtKB - P40968 (PRP17_YEAST)
Pre-mRNA-processing factor 17
CDC40
Functioni
May function in the second step of pre-mRNA splicing. Regulatory protein involved in replication and mitotic spindle formation and/or maintenance. Required for initiation and completion of S-phase and for initiation and completion of DNA replication. Might be required for the maintenance of microtubules. Essential only at elevated temperatures.
Miscellaneous
GO - Molecular functioni
- mRNA binding Source: GO_Central
GO - Biological processi
- generation of catalytic spliceosome for second transesterification step Source: SGD
- mRNA 3'-splice site recognition Source: SGD
- mRNA branch site recognition Source: SGD
- mRNA splicing, via spliceosome Source: GO_Central
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-processing factor 17Alternative name(s): Cell division control protein 40 |
Gene namesi | Name:CDC40 Synonyms:PRP17, SLU4, XRS2 Ordered Locus Names:YDR364C ORF Names:D9481.11 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002772, CDC40 |
VEuPathDBi | FungiDB:YDR364C |
Subcellular locationi
Nucleus
Nucleus
- catalytic step 2 spliceosome Source: GO_Central
- nuclear periphery Source: SGD
- spliceosomal complex Source: SGD
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051144 | 1 – 455 | Pre-mRNA-processing factor 17Add BLAST | 455 |
Proteomic databases
MaxQBi | P40968 |
PaxDbi | P40968 |
PRIDEi | P40968 |
PTM databases
iPTMneti | P40968 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
1 PublicationBinary interactionsi
P40968
With | #Exp. | IntAct |
---|---|---|
PRP19 [P32523] | 7 | EBI-13834,EBI-493 |
Protein-protein interaction databases
BioGRIDi | 32421, 164 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-1111N |
IntActi | P40968, 9 interactors |
MINTi | P40968 |
STRINGi | 4932.YDR364C |
Miscellaneous databases
RNActi | P40968, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P40968 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 160 – 200 | WD 1Add BLAST | 41 | |
Repeati | 204 – 243 | WD 2Add BLAST | 40 | |
Repeati | 291 – 330 | WD 3Add BLAST | 40 | |
Repeati | 334 – 373 | WD 4Add BLAST | 40 | |
Repeati | 379 – 422 | WD 5Add BLAST | 44 | |
Repeati | 424 – 454 | WD 6Add BLAST | 31 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 89 | DisorderedSequence analysisAdd BLAST | 89 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 49 | Basic and acidic residuesSequence analysisAdd BLAST | 36 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0282, Eukaryota |
GeneTreei | ENSGT00530000063583 |
HOGENOMi | CLU_022571_0_0_1 |
InParanoidi | P40968 |
OMAi | SGGYVCF |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR032847, PRPF17 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR43979, PTHR43979, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH
60 70 80 90 100
FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKEDQ DIFVHALAED
110 120 130 140 150
NLDSEQIEVE EVSHFYGKSE KDYQGRGYLY PPNDVDVDLR EERISFRCYL
160 170 180 190 200
PKKVIRNYPG HPEGTTALKF LPKTGHLILS GGNDHTIKIW DFYHDYECLR
210 220 230 240 250
DFQGHNKPIK ALRFTEDCQS FLSSSFDRSV KIWDTETGKV KTRLHLNSTP
260 270 280 290 300
ADVESRPTNP HEFIVGLSNS KILHYDDRVS ENQGLVQTYD HHLSSILALK
310 320 330 340 350
YFPDGSKFIS SSEDKTVRIW ENQINVPIKQ ISDTAQHSMP FLNVHPSQNY
360 370 380 390 400
FCAQSMDNRI YSFSLKPKYK RHPKKIFKGH SSAGYGISLA FSGDGRYICS
410 420 430 440 450
GDSKSRLFTW DWNTSRLLNN IKIPGNKPIT QVDWHPQETS KVICSGAAGK
IYVCD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 390 | A → T in AAA86875 (PubMed:7753021).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17018 Genomic DNA Translation: AAA86875.1 U28373 Genomic DNA Translation: AAB64800.1 BK006938 Genomic DNA Translation: DAA12203.1 |
PIRi | S61159 |
RefSeqi | NP_010652.3, NM_001180672.3 |
Genome annotation databases
EnsemblFungii | YDR364C_mRNA; YDR364C; YDR364C |
GeneIDi | 851968 |
KEGGi | sce:YDR364C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17018 Genomic DNA Translation: AAA86875.1 U28373 Genomic DNA Translation: AAB64800.1 BK006938 Genomic DNA Translation: DAA12203.1 |
PIRi | S61159 |
RefSeqi | NP_010652.3, NM_001180672.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5GM6 | electron microscopy | 3.50 | n | 1-455 | [»] | |
5GMK | electron microscopy | 3.40 | n | 1-455 | [»] | |
5MPS | electron microscopy | 3.85 | o | 1-455 | [»] | |
5MQ0 | electron microscopy | 4.17 | o | 1-455 | [»] | |
5WSG | electron microscopy | 4.00 | n | 1-455 | [»] | |
5Y88 | electron microscopy | 3.70 | S | 1-455 | [»] | |
5YLZ | electron microscopy | 3.60 | T | 1-455 | [»] | |
6BK8 | electron microscopy | 3.30 | M | 1-455 | [»] | |
6EXN | electron microscopy | 3.70 | o | 1-455 | [»] | |
6J6G | electron microscopy | 3.20 | n | 1-455 | [»] | |
6J6H | electron microscopy | 3.60 | n | 1-455 | [»] | |
6J6N | electron microscopy | 3.86 | n | 1-455 | [»] | |
6J6Q | electron microscopy | 3.70 | n | 1-455 | [»] | |
SMRi | P40968 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32421, 164 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-1111N |
IntActi | P40968, 9 interactors |
MINTi | P40968 |
STRINGi | 4932.YDR364C |
PTM databases
iPTMneti | P40968 |
Proteomic databases
MaxQBi | P40968 |
PaxDbi | P40968 |
PRIDEi | P40968 |
Genome annotation databases
EnsemblFungii | YDR364C_mRNA; YDR364C; YDR364C |
GeneIDi | 851968 |
KEGGi | sce:YDR364C |
Organism-specific databases
SGDi | S000002772, CDC40 |
VEuPathDBi | FungiDB:YDR364C |
Phylogenomic databases
eggNOGi | KOG0282, Eukaryota |
GeneTreei | ENSGT00530000063583 |
HOGENOMi | CLU_022571_0_0_1 |
InParanoidi | P40968 |
OMAi | SGGYVCF |
Miscellaneous databases
PROi | PR:P40968 |
RNActi | P40968, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR032847, PRPF17 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR43979, PTHR43979, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP17_YEAST | |
Accessioni | P40968Primary (citable) accession number: P40968 Secondary accession number(s): D6VSZ3, Q06341 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references