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Protein

Spindle assembly checkpoint component MAD1

Gene

MAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Central component of the spindle assembly checkpoint. Thought to recruit MAD2 to unattached kinetochores. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism.

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30587-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle assembly checkpoint component MAD1
Alternative name(s):
Mitotic arrest deficient protein 1
Gene namesi
Name:MAD1
Ordered Locus Names:YGL086W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL086W
SGDiS000003054 MAD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi653 – 655RLK → AAA: Abolishes interaction with BUB1 and BUB3 and spindle checkpoint function. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137981 – 749Spindle assembly checkpoint component MAD1Add BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei502PhosphothreonineCombined sources1

Post-translational modificationi

Becomes hyperphosphorylated when wild-type cells are arrested in mitosis. Phosphorylated by MPS1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40957
PaxDbiP40957
PRIDEiP40957

PTM databases

iPTMnetiP40957

Interactioni

Subunit structurei

Forms a stable heteromer with MAD2 throughout the cell cycle. Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi33164, 436 interactors
ComplexPortaliCPX-3212 Mitotic checkpoint complex, MAD1-MAD2-BUB1-BUB3 subcomplex
CPX-961 Mitotic spindle assembly checkpoint Mad1-Mad2 complex
DIPiDIP-293N
ELMiP40957
IntActiP40957, 12 interactors
MINTiP40957
STRINGi4932.YGL086W

Structurei

3D structure databases

ProteinModelPortaliP40957
SMRiP40957
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili57 – 324Sequence analysisAdd BLAST268
Coiled coili390 – 656Sequence analysisAdd BLAST267

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi323 – 329Poly-Asp7
Compositional biasi330 – 347Poly-AsnAdd BLAST18
Compositional biasi355 – 363Poly-Asn9
Compositional biasi601 – 604Poly-Leu4

Sequence similaritiesi

Belongs to the MAD1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000113453
InParanoidiP40957
KOiK06679
OMAiYMLLGYR
OrthoDBiEOG092C0U40

Family and domain databases

InterProiView protein in InterPro
IPR008672 Mad1
PANTHERiPTHR23168 PTHR23168, 1 hit
PfamiView protein in Pfam
PF05557 MAD, 1 hit

Sequencei

Sequence statusi: Complete.

P40957-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVRAALQCF FSALSGRFTG KKLGLEIYSI QYKMSNSGGS SPFLESPGGS
60 70 80 90 100
PDVGSTNGQS NRQIQALQFK LNTLQNEYEI EKLQLQKQTN ILEKKYKATI
110 120 130 140 150
DELEKALNDT KYLYESNDKL EQELKSLKER SANSMNDKDK CIEELRTTLQ
160 170 180 190 200
NKDLEMETLR QQYDSKLSKV TNQCDHFKLE AESSHSLLMK YEKEIKRQSV
210 220 230 240 250
DIKDLQHQVM EKDDELSSVK ASKMINSHPN YSTEEFNELT EMNKMIQDQV
260 270 280 290 300
QYTKELELAN MQQANELKKL KQSQDTSTFW KLENEKLQNK LSQLHVLESQ
310 320 330 340 350
YENLQLENID LKSKLTKWEI YNDSDDDDDN NVNNNDNNNN NKNDNNNDNN
360 370 380 390 400
NDTSNNNNIN NNNRTKNNIR NNPEEIIRDW KLTKKECLIL TDMNDKLRLD
410 420 430 440 450
NNNLKLLNDE MALERNQILD LNKNYENNIV NLKRLNHELE QQKSLSFEEC
460 470 480 490 500
RLLREQLDGL YSAQNNALLE VENSETHASN KNVNEDMNNL IDTYKNKTED
510 520 530 540 550
LTNELKKLND QLLSNSNDVE TQRKKRKLTS DQIGLNYSQR LNELQLENVS
560 570 580 590 600
VSRELSKAQT TIQLLQEKLE KLTKLKEKKI RILQLRDGPF IKDQFIKKNK
610 620 630 640 650
LLLLEKENAD LLNELKKNNP AVETVPISVY DSLNFELKQF EQEVFKSNKR
660 670 680 690 700
FSRLKQVFNN KSLEFIDVVN SLLGFKLEFQ QDSRVKIFSC FKPEKYLIAD
710 720 730 740
LNENTLKSNL DADIEGWDDL MNLWVEDRGQ LPCFLATITL RLWEQRQAK
Length:749
Mass (Da):87,651
Last modified:February 1, 1995 - v1
Checksum:i312DDE1BE241610D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14632 Genomic DNA Translation: AAA91620.1
Z72608 Genomic DNA Translation: CAA96791.1
BK006941 Genomic DNA Translation: DAA08019.1
PIRiA57276
RefSeqiNP_011429.3, NM_001180951.3

Genome annotation databases

EnsemblFungiiYGL086W; YGL086W; YGL086W
GeneIDi852794
KEGGisce:YGL086W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14632 Genomic DNA Translation: AAA91620.1
Z72608 Genomic DNA Translation: CAA96791.1
BK006941 Genomic DNA Translation: DAA08019.1
PIRiA57276
RefSeqiNP_011429.3, NM_001180951.3

3D structure databases

ProteinModelPortaliP40957
SMRiP40957
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33164, 436 interactors
ComplexPortaliCPX-3212 Mitotic checkpoint complex, MAD1-MAD2-BUB1-BUB3 subcomplex
CPX-961 Mitotic spindle assembly checkpoint Mad1-Mad2 complex
DIPiDIP-293N
ELMiP40957
IntActiP40957, 12 interactors
MINTiP40957
STRINGi4932.YGL086W

PTM databases

iPTMnetiP40957

Proteomic databases

MaxQBiP40957
PaxDbiP40957
PRIDEiP40957

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL086W; YGL086W; YGL086W
GeneIDi852794
KEGGisce:YGL086W

Organism-specific databases

EuPathDBiFungiDB:YGL086W
SGDiS000003054 MAD1

Phylogenomic databases

HOGENOMiHOG000113453
InParanoidiP40957
KOiK06679
OMAiYMLLGYR
OrthoDBiEOG092C0U40

Enzyme and pathway databases

BioCyciYEAST:G3O-30587-MONOMER

Miscellaneous databases

PROiPR:P40957

Family and domain databases

InterProiView protein in InterPro
IPR008672 Mad1
PANTHERiPTHR23168 PTHR23168, 1 hit
PfamiView protein in Pfam
PF05557 MAD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAD1_YEAST
AccessioniPrimary (citable) accession number: P40957
Secondary accession number(s): D6VU58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 10, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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Main funding by: National Institutes of Health

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