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UniProtKB - P40952 (KYE1_KLULA)
Protein
Enoate reductase 1
Gene
KYE1
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Functioni
Enoate reductase with broad substrate specificity for different alpha,beta-unsaturated carbonyl compounds. Prefers NADPH over NADH as cofactor.
Catalytic activityi
- EC:1.3.1.311 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | FMNBy similarity | 1 | |
Binding sitei | 191 | FMNBy similarity | 1 | |
Binding sitei | 191 | SubstrateBy similarity | 1 | |
Binding sitei | 194 | SubstrateBy similarity | 1 | |
Active sitei | 196 | Proton donorBy similarity | 1 | |
Binding sitei | 243 | FMNBy similarity | 1 | |
Binding sitei | 348 | FMNBy similarity | 1 | |
Binding sitei | 375 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 2-enoate reductase activity Source: UniProtKB-EC
- FMN binding Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN, NAD, NADP |
Enzyme and pathway databases
BRENDAi | 1.3.1.31, 2825 |
Names & Taxonomyi
Protein namesi | Recommended name: Enoate reductase 1Alternative name(s): Old yellow enzyme 1 |
Gene namesi | Name:KYE1 Ordered Locus Names:KLLA0A09075g |
Organismi | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
Taxonomic identifieri | 284590 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194476 | 1 – 398 | Enoate reductase 1Add BLAST | 398 |
Interactioni
Subunit structurei
Homodimer or heterodimer.
Protein-protein interaction databases
STRINGi | 28985.XP_451397.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.Curated
Phylogenomic databases
eggNOGi | KOG0134, Eukaryota |
HOGENOMi | CLU_012153_0_0_1 |
InParanoidi | P40952 |
OMAi | GQTPENM |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001155, OxRdtase_FMN_N IPR045247, Oye-like |
PANTHERi | PTHR22893, PTHR22893, 1 hit |
Pfami | View protein in Pfam PF00724, Oxidored_FMN, 1 hit |
i Sequence
Sequence statusi: Complete.
P40952-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFMNFEPKP LADTDIFKPI KIGNTELKHR VVMPALTRMR ALHPGNVPNP
60 70 80 90 100
DWAVEYYRQR SQYPGTMIIT EGAFPSAQSG GYDNAPGVWS EEQLAQWRKI
110 120 130 140 150
FKAIHDNKSF VWVQLWVLGR QAFADNLARD GLRYDSASDE VYMGEDEKER
160 170 180 190 200
AIRSNNPQHG ITKDEIKQYI RDYVDAAKKC IDAGADGVEI HSANGYLLNQ
210 220 230 240 250
FLDPISNKRT DEYGGSIENR ARFVLEVVDA VVDAVGAERT SIRFSPYGVF
260 270 280 290 300
GTMSGGSDPV LVAQFAYVLA ELEKRAKAGK RLAYVDLVEP RVTSPFQPEF
310 320 330 340 350
EGWYKGGTNE FVYSVWKGNV LRVGNYALDP DAAITDSKNP NTLIGYGRAF
360 370 380 390
IANPDLVERL EKGLPLNQYD RPSFYKMSAE GYIDYPTYEE AVAKGYKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 256 | G → V in AAA98815 (PubMed:7597850).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37452 Genomic DNA Translation: AAA98815.1 CR382121 Genomic DNA Translation: CAH02985.1 |
PIRi | S55844 |
RefSeqi | XP_451397.1, XM_451397.1 |
Genome annotation databases
EnsemblFungii | CAH02985; CAH02985; KLLA0_A09075g |
GeneIDi | 2896828 |
KEGGi | kla:KLLA0_A09075g |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37452 Genomic DNA Translation: AAA98815.1 CR382121 Genomic DNA Translation: CAH02985.1 |
PIRi | S55844 |
RefSeqi | XP_451397.1, XM_451397.1 |
3D structure databases
SMRi | P40952 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 28985.XP_451397.1 |
Genome annotation databases
EnsemblFungii | CAH02985; CAH02985; KLLA0_A09075g |
GeneIDi | 2896828 |
KEGGi | kla:KLLA0_A09075g |
Phylogenomic databases
eggNOGi | KOG0134, Eukaryota |
HOGENOMi | CLU_012153_0_0_1 |
InParanoidi | P40952 |
OMAi | GQTPENM |
Enzyme and pathway databases
BRENDAi | 1.3.1.31, 2825 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001155, OxRdtase_FMN_N IPR045247, Oye-like |
PANTHERi | PTHR22893, PTHR22893, 1 hit |
Pfami | View protein in Pfam PF00724, Oxidored_FMN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KYE1_KLULA | |
Accessioni | P40952Primary (citable) accession number: P40952 Secondary accession number(s): Q6CXE2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | September 27, 2004 | |
Last modified: | February 23, 2022 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families