UniProtKB - P40949 (TAPA_BACSU)
Protein
TasA anchoring/assembly protein
Gene
tapA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Required for biofilm formation (PubMed:16430695, PubMed:16430696, PubMed:21477127, PubMed:24488317). Required for the proper anchoring and polymerization of TasA amyloid fibers at the cell surface (PubMed:16430696, PubMed:21477127, PubMed:24488317). Is also a minor component of TasA fibers (PubMed:21477127).4 Publications
Enzyme and pathway databases
BioCyci | BSUB:BSU24640-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: TasA anchoring/assembly protein1 PublicationAlternative name(s): Biofilm assembly accessory protein TapACurated |
Gene namesi | Name:tapA1 Publication Synonyms:yqhD, yqxM Ordered Locus Names:BSU24640 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- bacterial biofilm matrix Source: InterPro
- cell wall Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell wall, SecretedPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
ChainiPRO_0000049851 | 33 – 253 | TasA anchoring/assembly proteinAdd BLAST | 221 |
Proteomic databases
PaxDbi | P40949 |
PRIDEi | P40949 |
Expressioni
Inductioni
Part of the tapA-sipW-tasA operon (PubMed:10464223). Expression is directly repressed by the DNA-binding protein master regulator of biofilm formation SinR and activated by the extracellular matrix regulatory protein RemA (PubMed:16430695, PubMed:23646920). Also positively regulated by the sporulation transcription factors sigma H and Spo0A and repressed by the transition phase regulatory protein AbrB, probably indirectly (PubMed:10464223). Induced by a high concentration of salt (PubMed:10559173). During most conditions of growth, may be present at very low levels or is not synthesized at all, due, at least in part, to post-transcriptional repression (PubMed:10559173).4 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P40949 |
SMRi | P40949 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 50 – 57 | Important for TasA fiber formation1 Publication | 8 |
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG5030CJ7, Bacteria |
OMAi | WKWELHK |
Family and domain databases
InterProi | View protein in InterPro IPR023833, Signal_peptide_camelysin IPR023848, TasA |
TIGRFAMsi | TIGR04088, cognate_SipW, 1 hit TIGR04087, YqxM_for_SipW, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P40949-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRLFHNQQK AKTKLKVLLI FQLSVIFSLT AAICLQFSDD TSAAFHDIET
60 70 80 90 100
FDVSLQTCKD FQHTDKNCHY DKRWDQSDLH ISDQTDTKGT VCSPFALFAV
110 120 130 140 150
LENTGEKLKK SKWKWELHKL ENARKPLKDG NVIEKGFVSN QIGDSLYKIE
160 170 180 190 200
TKKKMKPGIY AFKVYKPAGY PANGSTFEWS EPMRLAKCDE KPTVPKKETK
210 220 230 240 250
SDVKKENETT QKDIPEKTMK EETSQEAVTK EKETQSDQKE SGEEDEKSNE
ADQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 39 – 51 | DDTSA…DIETF → MIQALLFMILKHL in AAA83374 (PubMed:2507524).CuratedAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12539.1 AL009126 Genomic DNA Translation: CAB14395.1 M29691 Genomic DNA Translation: AAA83374.1 |
PIRi | G69968 |
RefSeqi | NP_390344.1, NC_000964.3 WP_004399106.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14395; CAB14395; BSU24640 |
GeneIDi | 938532 |
KEGGi | bsu:BSU24640 |
PATRICi | fig|224308.179.peg.2682 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12539.1 AL009126 Genomic DNA Translation: CAB14395.1 M29691 Genomic DNA Translation: AAA83374.1 |
PIRi | G69968 |
RefSeqi | NP_390344.1, NC_000964.3 WP_004399106.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HQC | X-ray | 1.28 | A | 75-190 | [»] | |
6QAY | NMR | - | A | 44-190 | [»] | |
BMRBi | P40949 | |||||
SMRi | P40949 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU24640 |
Proteomic databases
PaxDbi | P40949 |
PRIDEi | P40949 |
Genome annotation databases
EnsemblBacteriai | CAB14395; CAB14395; BSU24640 |
GeneIDi | 938532 |
KEGGi | bsu:BSU24640 |
PATRICi | fig|224308.179.peg.2682 |
Phylogenomic databases
eggNOGi | ENOG5030CJ7, Bacteria |
OMAi | WKWELHK |
Enzyme and pathway databases
BioCyci | BSUB:BSU24640-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR023833, Signal_peptide_camelysin IPR023848, TasA |
TIGRFAMsi | TIGR04088, cognate_SipW, 1 hit TIGR04087, YqxM_for_SipW, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TAPA_BACSU | |
Accessioni | P40949Primary (citable) accession number: P40949 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 102 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references