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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.1 Publication
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+ = 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei105NADBy similarity1
Binding sitei112NADBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei162Substrate1
Active sitei187Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17NADBy similarity7
Nucleotide bindingi129 – 131NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:HS00361-MONOMER
ReactomeiR-HSA-70263 Gluconeogenesis
SABIO-RKiP40925

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Diiodophenylpyruvate reductase (EC:1.1.1.96)
Gene namesi
Name:MDH1
Synonyms:MDHA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000014641.17
HGNCiHGNC:6970 MDH1
MIMi154200 gene
neXtProtiNX_P40925

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi4190
OpenTargetsiENSG00000014641
PharmGKBiPA30714

Chemistry databases

ChEMBLiCHEMBL3560
DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
DB00157 NADH

Polymorphism and mutation databases

BioMutaiMDH1
DMDMi1708967

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001134092 – 334Malate dehydrogenase, cytoplasmicAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei110N6-succinyllysineBy similarity1
Modified residuei118N6-acetyllysineCombined sources1 Publication1
Modified residuei121N6-acetyllysine1 Publication1
Modified residuei214N6-succinyllysineBy similarity1
Modified residuei217PhosphoserineBy similarity1
Modified residuei230Omega-N-methylarginineBy similarity1
Modified residuei241PhosphoserineCombined sources1
Modified residuei298N6-acetyllysine; alternateCombined sources1 Publication1
Modified residuei298N6-succinyllysine; alternateBy similarity1
Modified residuei309PhosphoserineBy similarity1
Modified residuei318N6-succinyllysineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Modified residuei333PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.1 Publication
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.2 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP40925
PaxDbiP40925
PeptideAtlasiP40925
PRIDEiP40925
ProteomicsDBi55384
55385 [P40925-2]
TopDownProteomicsiP40925-1 [P40925-1]

2D gel databases

REPRODUCTION-2DPAGEiIPI00291005
UCD-2DPAGEiP40925

PTM databases

iPTMnetiP40925
PhosphoSitePlusiP40925
SwissPalmiP40925

Expressioni

Gene expression databases

BgeeiENSG00000014641
CleanExiHS_MDH1
ExpressionAtlasiP40925 baseline and differential
GenevisibleiP40925 HS

Organism-specific databases

HPAiCAB047333
HPA027296
HPA054276

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
TERF1P542742EBI-709625,EBI-710997

Protein-protein interaction databases

BioGridi110355, 40 interactors
IntActiP40925, 12 interactors
MINTiP40925
STRINGi9606.ENSP00000438144

Chemistry databases

BindingDBiP40925

Structurei

3D structure databases

ProteinModelPortaliP40925
SMRiP40925
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiKOG1496 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00530000063410
HOGENOMiHOG000220953
HOVERGENiHBG006340
InParanoidiP40925
KOiK00025
OMAiRPRTKGM
OrthoDBiEOG091G0BLA
PhylomeDBiP40925
TreeFamiTF105826

Family and domain databases

CDDicd01336 MDH_cytoplasmic_cytosolic, 1 hit
Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR011274 Malate_DH_NAD-dep_euk
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit
TIGR01758 MDH_euk_cyt, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P40925-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD
110 120 130 140 150
LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV
310 320 330
EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSA
Length:334
Mass (Da):36,426
Last modified:January 23, 2007 - v4
Checksum:i5F7ED9789CA1DB55
GO
Isoform 2 (identifier: P40925-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: Missing.

Show »
Length:245
Mass (Da):27,025
Checksum:iDEBC412374575BD9
GO
Isoform 3 (identifier: P40925-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRRCSYFPKDVTVFDKDDK

Note: No experimental confirmation available.
Show »
Length:352
Mass (Da):38,628
Checksum:i058B25735B30BBC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15Q → R in BAH12223 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0426611 – 89Missing in isoform 2. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_0458471M → MRRCSYFPKDVTVFDKDDK in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55654 mRNA Translation: BAA09513.1
U20352 mRNA Translation: AAC16436.1
CR457405 mRNA Translation: CAG33686.1
AK295931 mRNA Translation: BAH12223.1
AK300719 mRNA Translation: BAG62394.1
AK312331 mRNA Translation: BAG35252.1
AC016734 Genomic DNA Translation: AAY14893.1
CH471053 Genomic DNA Translation: EAW99959.1
BC001484 mRNA Translation: AAH01484.1
CCDSiCCDS1874.1 [P40925-1]
CCDS56121.1 [P40925-3]
CCDS56122.1 [P40925-2]
PIRiG01650
RefSeqiNP_001186040.1, NM_001199111.1 [P40925-3]
NP_001186041.1, NM_001199112.1 [P40925-2]
NP_001303303.1, NM_001316374.1
NP_005908.1, NM_005917.3 [P40925-1]
UniGeneiHs.526521

Genome annotation databases

EnsembliENST00000233114; ENSP00000233114; ENSG00000014641 [P40925-1]
ENST00000539945; ENSP00000438144; ENSG00000014641 [P40925-3]
ENST00000544381; ENSP00000446395; ENSG00000014641 [P40925-2]
GeneIDi4190
KEGGihsa:4190
UCSCiuc010ypv.3 human [P40925-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMDHC_HUMAN
AccessioniPrimary (citable) accession number: P40925
Secondary accession number(s): B2R5V5
, B4DUN2, B7Z3I7, F5H098, Q6I9V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 189 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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