UniProtKB - P40897 (OPT1_YEAST)
Protein
Oligopeptide transporter 1
Gene
OPT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
High affinity transporter for glutathione. Also transports tetra- and pentapeptides like the opioids leucine enkephalin (Tyr-Gly-Gly-Phe-Leu) and methionine enkephalin (Tyr-Gly-Gly_Phe-Met) across the cell membrane.2 Publications
Miscellaneous
Present with 2870 molecules/cell in log phase SD medium.1 Publication
Kineticsi
- KM=53.9 µM for glutathione2 Publications
- KM=310 µM for leucine enkephalin2 Publications
- Vmax=10 nmol/min/mg enzyme for glutathione2 Publications
pH dependencei
Optimum pH is 5.5.2 Publications
GO - Molecular functioni
- oligopeptide transmembrane transporter activity Source: SGD
- proton-dependent oligopeptide secondary active transmembrane transporter activity Source: SGD
GO - Biological processi
- oligopeptide transport Source: SGD
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Peptide transport, Protein transport, Transport |
Enzyme and pathway databases
SABIO-RKi | P40897 |
Protein family/group databases
TCDBi | 2.A.67.1.3, the oligopeptide transporter (opt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Oligopeptide transporter 1Alternative name(s): High affinity glutathione transporter 1 |
Gene namesi | Name:OPT1 Synonyms:GSH11, HGT1 Ordered Locus Names:YJL212C ORF Names:HRD799, J0236 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YJL212C |
SGDi | S000003748, OPT1 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 108 | ExtracellularSequence analysisAdd BLAST | 108 | |
Transmembranei | 109 – 129 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 130 – 135 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 136 – 156 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 157 – 177 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 178 – 198 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 199 – 210 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 211 – 231 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 232 – 276 | ExtracellularSequence analysisAdd BLAST | 45 | |
Transmembranei | 277 – 297 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 298 – 313 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 314 – 334 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 335 – 359 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 360 – 380 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 381 – 428 | CytoplasmicSequence analysisAdd BLAST | 48 | |
Transmembranei | 429 – 449 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 450 – 482 | ExtracellularSequence analysisAdd BLAST | 33 | |
Transmembranei | 483 – 503 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 504 – 508 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 509 – 529 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 530 – 540 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 541 – 561 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 562 – 590 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 591 – 611 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 612 – 659 | ExtracellularSequence analysisAdd BLAST | 48 | |
Transmembranei | 660 – 680 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 681 – 736 | CytoplasmicSequence analysisAdd BLAST | 56 | |
Transmembranei | 737 – 757 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 758 – 799 | ExtracellularSequence analysisAdd BLAST | 42 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213788 | 1 – 799 | Oligopeptide transporter 1Add BLAST | 799 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 46 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 48 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 50 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 51 | PhosphothreonineCombined sources | 1 | |
Glycosylationi | 640 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P40897 |
PaxDbi | P40897 |
PRIDEi | P40897 |
PTM databases
iPTMneti | P40897 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33547, 57 interactors |
DIPi | DIP-5691N |
STRINGi | 4932.YJL212C |
Miscellaneous databases
RNActi | P40897, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the oligopeptide OPT transporter family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2262, Eukaryota |
GeneTreei | ENSGT00940000176656 |
HOGENOMi | CLU_004965_1_1_1 |
InParanoidi | P40897 |
OMAi | NQHVGLN |
Family and domain databases
InterProi | View protein in InterPro IPR004648, Oligpept_transpt IPR004813, OPT |
Pfami | View protein in Pfam PF03169, OPT, 1 hit |
TIGRFAMsi | TIGR00727, ISP4_OPT, 1 hit TIGR00728, OPT_sfam, 1 hit |
i Sequence
Sequence statusi: Complete.
P40897-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTIYRESDS LESEPSPTPT TIPIQINMEE EKKDAFVKNI DEDVNNLTAT
60 70 80 90 100
TDEEDRDPES QKFDRHSIQE EGLVWKGDPT YLPNSPYPEV RSAVSIEDDP
110 120 130 140 150
TIRLNHWRTW FLTTVFVVVF AGVNQFFSLR YPSLEINFLV AQVVCYPIGR
160 170 180 190 200
ILALLPDWKC SKVPFFDLNP GPFTKKEHAV VTIAVALTSS TAYAMYILNA
210 220 230 240 250
QGSFYNMKLN VGYQFLLVWT SQMIGYGAAG LTRRWVVNPA SSIWPQTLIS
260 270 280 290 300
VSLFDSLHSR KVEKTVANGW TMPRYRFFLI VLIGSFIWYW VPGFLFTGLS
310 320 330 340 350
YFNVILWGSK TRHNFIANTI FGTQSGLGAL PITFDYTQVS QAMSGSVFAT
360 370 380 390 400
PFYVSANTYA SVLIFFVIVL PCLYFTNTWY AKYMPVISGS TYDNTQNKYN
410 420 430 440 450
VTKILNEDYS INLEKYKEYS PVFVPFSYLL SYALNFAAVI AVFVHCILYH
460 470 480 490 500
GKDIVAKFKD RKNGGTDIHM RIYSKNYKDC PDWWYLLLQI VMIGLGFVAV
510 520 530 540 550
CCFDTKFPAW AFVIAILISL VNFIPQGILE AMTNQHVGLN IITELICGYM
560 570 580 590 600
LPLRPMANLL FKLYGFIVMR QGLNLSRDLK LAMYMKVSPR LIFAVQIYAT
610 620 630 640 650
IISGMVNVGV QEWMMHNIDG LCTTDQPNGF TCANGRTVFN ASIIWSLPKY
660 670 680 690 700
LFSSGRIYNP LMWFFLIGLL FPLAVYAVQW KFPKFKFAKH IHTPVFFTGP
710 720 730 740 750
GNIPPSTPYN YSLFFAMSFC LNLIRKRWRA WFNKYNFVMG AGVEAGVAIS
760 770 780 790
VVIIFLCVQY PGGKLSWWGN NVWKRTYDND YKKFYTLKKG ETFGYDKWW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z34098 Genomic DNA Translation: CAA83999.1 Z49487 Genomic DNA Translation: CAA89509.1 BK006943 Genomic DNA Translation: DAA08599.1 |
PIRi | S50773 |
RefSeqi | NP_012323.1, NM_001181645.1 |
Genome annotation databases
EnsemblFungii | YJL212C_mRNA; YJL212C; YJL212C |
GeneIDi | 853218 |
KEGGi | sce:YJL212C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z34098 Genomic DNA Translation: CAA83999.1 Z49487 Genomic DNA Translation: CAA89509.1 BK006943 Genomic DNA Translation: DAA08599.1 |
PIRi | S50773 |
RefSeqi | NP_012323.1, NM_001181645.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33547, 57 interactors |
DIPi | DIP-5691N |
STRINGi | 4932.YJL212C |
Protein family/group databases
TCDBi | 2.A.67.1.3, the oligopeptide transporter (opt) family |
PTM databases
iPTMneti | P40897 |
Proteomic databases
MaxQBi | P40897 |
PaxDbi | P40897 |
PRIDEi | P40897 |
Genome annotation databases
EnsemblFungii | YJL212C_mRNA; YJL212C; YJL212C |
GeneIDi | 853218 |
KEGGi | sce:YJL212C |
Organism-specific databases
EuPathDBi | FungiDB:YJL212C |
SGDi | S000003748, OPT1 |
Phylogenomic databases
eggNOGi | KOG2262, Eukaryota |
GeneTreei | ENSGT00940000176656 |
HOGENOMi | CLU_004965_1_1_1 |
InParanoidi | P40897 |
OMAi | NQHVGLN |
Enzyme and pathway databases
SABIO-RKi | P40897 |
Miscellaneous databases
PROi | PR:P40897 |
RNActi | P40897, protein |
Family and domain databases
InterProi | View protein in InterPro IPR004648, Oligpept_transpt IPR004813, OPT |
Pfami | View protein in Pfam PF03169, OPT, 1 hit |
TIGRFAMsi | TIGR00727, ISP4_OPT, 1 hit TIGR00728, OPT_sfam, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPT1_YEAST | |
Accessioni | P40897Primary (citable) accession number: P40897 Secondary accession number(s): D6VVY3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | October 7, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names