UniProtKB - P40874 (MTOX_ECOLI)
Protein
N-methyl-L-tryptophan oxidase
Gene
solA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the oxidative demethylation of N-methyl-L-tryptophan. Can also use other N-methyl amino acids, including sarcosine, which, however, is a poor substrate.
Catalytic activityi
Cofactori
FADNote: Binds 1 FAD per subunit.
Activity regulationi
Aromatic carboxylates are competitive inhibitors.
pH dependencei
Optimum pH is 8.0.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 4 – 34 | FADSequence analysisAdd BLAST | 31 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: EcoCyc
- N-methyl-L-amino-acid oxidase activity Source: EcoCyc
- sarcosine oxidase activity Source: GO_Central
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | EcoCyc:SARCOX-MONOMER MetaCyc:SARCOX-MONOMER |
SABIO-RKi | P40874 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:solA Ordered Locus Names:b1059, JW1046 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213765 | 1 – 372 | N-methyl-L-tryptophan oxidaseAdd BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 308 | S-8alpha-FAD cysteine | 1 |
Proteomic databases
jPOSTi | P40874 |
PaxDbi | P40874 |
PRIDEi | P40874 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 4261754, 81 interactors |
IntActi | P40874, 9 interactors |
STRINGi | 511145.b1059 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40874 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40874 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0665, Bacteria |
HOGENOMi | CLU_007884_2_1_6 |
InParanoidi | P40874 |
PhylomeDBi | P40874 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00515, MTOX, 1 hit |
InterProi | View protein in InterPro IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR023493, Me_Trp_Oxase_MTOX |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
P40874-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKYDLIIIGS GSVGAAAGYY ATRAGLNVLM TDAHMPPHQH GSHHGDTRLI
60 70 80 90 100
RHAYGEGEKY VPLVLRAQTL WDELSRHNEE DPIFVRSGVI NLGPADSTFL
110 120 130 140 150
ANVAHSAEQW QLNVEKLDAQ GIMARWPEIR VPDNYIGLFE TDSGFLRSEL
160 170 180 190 200
AIKTWIQLAK EAGCAQLFNC PVTAIRHDDD GVTIETADGE YQAKKAIVCA
210 220 230 240 250
GTWVKDLLPE LPVQPVRKVF AWYQADGRYS VKNKFPAFTG ELPNGDQYYG
260 270 280 290 300
FPAENDALKI GKHNGGQVIH SADERVPFAE VASDGSEAFP FLRNVLPGIG
310 320 330 340 350
CCLYGAACTY DNSPDEDFII DTLPGHDNTL LITGLSGHGF KFASVLGEIA
360 370
ADFAQDKKSD FDLTPFRLSR FQ
Mass spectrometryi
Molecular mass is 41684 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31709 Genomic DNA Translation: BAA06516.1 U00096 Genomic DNA Translation: AAC74143.1 AP009048 Genomic DNA Translation: BAA35856.1 |
PIRi | JC5371 |
RefSeqi | NP_415577.1, NC_000913.3 WP_000872833.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74143; AAC74143; b1059 BAA35856; BAA35856; BAA35856 |
GeneIDi | 944983 |
KEGGi | ecj:JW1046 eco:b1059 |
PATRICi | fig|1411691.4.peg.1209 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31709 Genomic DNA Translation: BAA06516.1 U00096 Genomic DNA Translation: AAC74143.1 AP009048 Genomic DNA Translation: BAA35856.1 |
PIRi | JC5371 |
RefSeqi | NP_415577.1, NC_000913.3 WP_000872833.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2UZZ | X-ray | 3.20 | A/B/C/D | 1-372 | [»] | |
SMRi | P40874 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261754, 81 interactors |
IntActi | P40874, 9 interactors |
STRINGi | 511145.b1059 |
Proteomic databases
jPOSTi | P40874 |
PaxDbi | P40874 |
PRIDEi | P40874 |
Genome annotation databases
EnsemblBacteriai | AAC74143; AAC74143; b1059 BAA35856; BAA35856; BAA35856 |
GeneIDi | 944983 |
KEGGi | ecj:JW1046 eco:b1059 |
PATRICi | fig|1411691.4.peg.1209 |
Organism-specific databases
EchoBASEi | EB2535 |
Phylogenomic databases
eggNOGi | COG0665, Bacteria |
HOGENOMi | CLU_007884_2_1_6 |
InParanoidi | P40874 |
PhylomeDBi | P40874 |
Enzyme and pathway databases
BioCyci | EcoCyc:SARCOX-MONOMER MetaCyc:SARCOX-MONOMER |
SABIO-RKi | P40874 |
Miscellaneous databases
EvolutionaryTracei | P40874 |
PROi | PR:P40874 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00515, MTOX, 1 hit |
InterProi | View protein in InterPro IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR023493, Me_Trp_Oxase_MTOX |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTOX_ECOLI | |
Accessioni | P40874Primary (citable) accession number: P40874 Secondary accession number(s): Q47144 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families