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UniProtKB - P40871 (DHBE_BACSU)
Protein
2,3-dihydroxybenzoate-AMP ligase
Gene
dhbE
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the biosynthesis of the catecholic siderophore bacillibactin. Catalyzes the activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate.
1 PublicationCatalytic activityi
- 2,3-dihydroxybenzoate + ATP + holo-[aryl-carrier protein] = 2,3-dihydroxybenzoyl-[aryl-carrier protein] + AMP + diphosphateBy similarityEC:6.2.1.71By similarity
: bacillibactin biosynthesis Pathwayi
This protein is involved in the pathway bacillibactin biosynthesis, which is part of Siderophore biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway bacillibactin biosynthesis and in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 191 | ATP; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 240 | Substrate1 Publication | 1 | |
Binding sitei | 307 | ATP; via amide nitrogen1 Publication | 1 | |
Binding sitei | 329 | ATP; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 413 | ATP1 Publication | 1 | |
Binding sitei | 428 | ATP1 Publication | 1 | |
Binding sitei | 519 | ATP1 Publication | 1 | |
Binding sitei | 519 | Substrate1 Publication | 1 |
GO - Molecular functioni
- (2,3-dihydroxybenzoyl)adenylate synthase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
GO - Biological processi
- siderophore biosynthetic process Source: InterPro
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU31980-MONOMER |
SABIO-RKi | P40871 |
UniPathwayi | UPA00013 |
Names & Taxonomyi
Protein namesi | Recommended name: 2,3-dihydroxybenzoate-AMP ligase1 Publication (EC:6.2.1.71By similarity)Alternative name(s): Dihydroxybenzoic acid-activating enzyme |
Gene namesi | Name:dhbE1 Publication Synonyms:entE Ordered Locus Names:BSU31980 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193074 | 1 – 539 | 2,3-dihydroxybenzoate-AMP ligaseAdd BLAST | 539 |
Proteomic databases
jPOSTi | P40871 |
PaxDbi | P40871 |
PRIDEi | P40871 |
Interactioni
Protein-protein interaction databases
IntActi | P40871, 1 interactor |
MINTi | P40871 |
STRINGi | 224308.BSU31980 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P40871 |
SMRi | P40871 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40871 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 234 – 235 | Substrate binding1 Publication | 2 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | COG1021, Bacteria |
InParanoidi | P40871 |
OMAi | WHEAAVI |
PhylomeDBi | P40871 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR011963, DHB_AMP_lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02275, DHB_AMP_lig, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P40871-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKGFTPWPD ELAETYRKNG CWAGETFGDL LRDRAAKYGD RIAITCGNTH
60 70 80 90 100
WSYRELDTRA DRLAAGFQKL GIQQMDRVVV QLPNIKEFFE VIFALFRLGA
110 120 130 140 150
LPVFALPSHR SSEITYFCEF AEAAAYIIPD AYSGFDYRSL ARQVQSKLPT
160 170 180 190 200
LKNIIVAGEA EEFLPLEDLH AEPVKLPEVK SSDVAFLQLS GGSTGLSKLI
210 220 230 240 250
PRTHDDYIYS LKRSVEVCWL DHSTVYLAAL PMAHNYPLSS PGVLGVLYAG
260 270 280 290 300
GRVVLSPSPS PDDAFPLIER EKVTITALVP PLAMVWMDAA SSRRDDLSSL
310 320 330 340 350
QVLQVGGAKF SAEAARRVKA VFGCTLQQVF GMAEGLVNYT RLDDPEEIIV
360 370 380 390 400
NTQGKPMSPY DEMRVWDDHD RDVKPGETGH LLTRGPYTIR GYYKAEEHNA
410 420 430 440 450
ASFTEDGFYR TGDIVRLTRD GYIVVEGRAK DQINRGGEKV AAEEVENHLL
460 470 480 490 500
AHPAVHDAAM VSMPDQFLGE RSCVFIIPRD EAPKAAELKA FLRERGLAAY
510 520 530
KIPDRVEFVE SFPQTGVGKV SKKALREAIS EKLLAGFKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 360 | Y → I in AAA79759 (PubMed:8224884).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U26444 Genomic DNA Translation: AAC44632.1 AL009126 Genomic DNA Translation: CAB15188.1 L08645 Genomic DNA Translation: AAA79759.1 |
PIRi | D69615 |
RefSeqi | NP_391078.1, NC_000964.3 WP_003243083.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15188; CAB15188; BSU_31980 |
GeneIDi | 936509 |
KEGGi | bsu:BSU31980 |
PATRICi | fig|224308.179.peg.3464 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U26444 Genomic DNA Translation: AAC44632.1 AL009126 Genomic DNA Translation: CAB15188.1 L08645 Genomic DNA Translation: AAA79759.1 |
PIRi | D69615 |
RefSeqi | NP_391078.1, NC_000964.3 WP_003243083.1, NZ_JNCM01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MD9 | X-ray | 2.80 | A | 1-539 | [»] | |
1MDB | X-ray | 2.15 | A | 1-539 | [»] | |
1MDF | X-ray | 2.50 | A | 1-539 | [»] | |
AlphaFoldDBi | P40871 | |||||
SMRi | P40871 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P40871, 1 interactor |
MINTi | P40871 |
STRINGi | 224308.BSU31980 |
Chemistry databases
DrugBanki | DB01672, 2,3-Dihydroxy-Benzoic Acid |
Proteomic databases
jPOSTi | P40871 |
PaxDbi | P40871 |
PRIDEi | P40871 |
Genome annotation databases
EnsemblBacteriai | CAB15188; CAB15188; BSU_31980 |
GeneIDi | 936509 |
KEGGi | bsu:BSU31980 |
PATRICi | fig|224308.179.peg.3464 |
Phylogenomic databases
eggNOGi | COG1021, Bacteria |
InParanoidi | P40871 |
OMAi | WHEAAVI |
PhylomeDBi | P40871 |
Enzyme and pathway databases
UniPathwayi | UPA00013 |
BioCyci | BSUB:BSU31980-MONOMER |
SABIO-RKi | P40871 |
Miscellaneous databases
EvolutionaryTracei | P40871 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR011963, DHB_AMP_lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02275, DHB_AMP_lig, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHBE_BACSU | |
Accessioni | P40871Primary (citable) accession number: P40871 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families