UniProtKB - P40791 (MEF2_DROME)
Protein
Myocyte-specific enhancer factor 2
Gene
Mef2
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Transcription factor that could be a key player in early mesoderm differentiation and may be required for subsequent cell fate specifications within the somatic and visceral/heart mesodermal layers (PubMed:7839146, PubMed:8052612, PubMed:8202544). Essential for myoblast fusion and consequently muscle formation in adults (PubMed:25797154). During embryonic and pupal development, binds to the enhancer of the myoblast fusion gene sing and activates its transcription (PubMed:25797154).4 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 58 – 86 | Mef2-typeSequence analysisAdd BLAST | 29 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: FlyBase
- DNA binding Source: FlyBase
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: FlyBase
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- histone deacetylase binding Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: GO_Central
- transcription factor binding Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- anterior midgut development Source: FlyBase
- antimicrobial humoral response Source: FlyBase
- carbohydrate storage Source: FlyBase
- cell differentiation Source: GO_Central
- heart development Source: FlyBase
- lipid storage Source: FlyBase
- locomotor rhythm Source: FlyBase
- mesoderm development Source: FlyBase
- muscle fiber development Source: FlyBase
- muscle organ development Source: FlyBase
- myoblast fusion Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- positive regulation of transcription elongation from RNA polymerase II promoter Source: FlyBase
- regulation of gene expression Source: FlyBase
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Myogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-525793, Myogenesis |
SignaLinki | P40791 |
Names & Taxonomyi
Protein namesi | Recommended name: Myocyte-specific enhancer factor 2Short name: D-mef2 Alternative name(s): MADS domain transcription factor |
Gene namesi | Name:Mef2 ORF Names:CG1429 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0011656, Mef2 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nuclear chromatin Source: GO_Central
- nucleus Source: FlyBase
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown in pupae results in smaller muscle founder templates that display a reduced number of nuclei. No effect on the initiation of myoblast fusion.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199427 | 1 – 540 | Myocyte-specific enhancer factor 2Add BLAST | 540 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 95 | Phosphoserine1 Publication | 1 | |
Modified residuei | 98 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinPTM databases
iPTMneti | P40791 |
Expressioni
Tissue specificityi
Expressed in all presumptive mesoderm prior to the splitting process that generates the somatic and visceral/ heart mesoderm. After the subdivision, it is found in both the somatic and the visceral/heart mesoderm.3 Publications
Developmental stagei
First detectable in the presumptive mesoderm at late cellular blastoderm stage.2 Publications
Inductioni
TWI activity is required for MEF2 expression. SNA activity is needed for maintaining MEF2 expression.
Gene expression databases
Bgeei | FBgn0011656, Expressed in spermathecum and 62 other tissues |
ExpressionAtlasi | P40791, differential |
Genevisiblei | P40791, DM |
Interactioni
Binary interactionsi
P40791
With | #Exp. | IntAct |
---|---|---|
Tbp [P20227] | 2 | EBI-235994,EBI-169179 |
GO - Molecular functioni
- histone deacetylase binding Source: GO_Central
- protein dimerization activity Source: InterPro
- transcription factor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 61860, 26 interactors |
IntActi | P40791, 3 interactors |
STRINGi | 7227.FBpp0087529 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 57 | MADS-boxPROSITE-ProRule annotationAdd BLAST | 55 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 377 – 431 | Gly-richAdd BLAST | 55 | |
Compositional biasi | 380 – 386 | Poly-Ser | 7 | |
Compositional biasi | 390 – 396 | Poly-Gly | 7 | |
Compositional biasi | 419 – 428 | Poly-Gly | 10 | |
Compositional biasi | 508 – 513 | Poly-Gln | 6 | |
Compositional biasi | 516 – 519 | Poly-Gln | 4 |
Sequence similaritiesi
Belongs to the MEF2 family.Curated
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
GeneTreei | ENSGT00940000169350 |
InParanoidi | P40791 |
OMAi | LVMPNSR |
PhylomeDBi | P40791 |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR022102, HJURP_C IPR033896, MADS_MEF2-like IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF12347, HJURP_C, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform D (identifier: P40791-1) [UniParc]FASTAAdd to basket
Also known as: 1
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRKKIQISR ITDERNRQVT FNKRKFGVMK KAYELSVLCD CEIALIIFSS
60 70 80 90 100
SNKLYQYAST DMDRVLLKYT EYNEPHESLT NKNIIEKENK NGVMSPDSPE
110 120 130 140 150
AETDYTLTPR TEAKYNKIDE EFQNMMQRNQ MAIGGAGAPR QLPNSSYTLP
160 170 180 190 200
VSVPVPGSYG DNLLQASPQM SHTNISPRPS SSETDSGGMS LIIYPSGSML
210 220 230 240 250
EMSNGYPHSH SPLVGSPSPG PSPGIAHHLS IKQQSPGSQN GRASNLRVVI
260 270 280 290 300
PPTIAPIPPN MSAPDDVGYA DQRQSQTSLN TPVVTLQTPI PALTSYSFGA
310 320 330 340 350
QDFSSSGVMN SADIMSLNTW HQGLVPHSSL SHLAVSNSTP PPATSPVSIK
360 370 380 390 400
VKAEPQSPPR DLSASGHQQN SNGSTGSGGS SSSTSSNASG GAGGGGAVSA
410 420 430 440 450
ANVITHLNNV SVLAGGPSGQ GGGGGGGGSN GNVEQATNLS VLSHAQQHHL
460 470 480 490 500
GMPNSRPSST GHITPTPGHD KYEGYPYRAL MGHNPRWNLN FAGAPSSDQD
510 520 530 540
VRLAAVAVQQ QQQQPHQQQQ LGDYDAPNHK RPRISGGWGT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4KEP4 | A0A0B4KEP4_DROME | Myocyte enhancer factor 2, isoform ... | Mef2 22.21, BEST:SD04091, C, D-MEF2, D-Mef2 | 511 | Annotation score: | ||
A0A0B4KFI1 | A0A0B4KFI1_DROME | Myocyte enhancer factor 2, isoform ... | Mef2 22.21, BEST:SD04091, C, D-MEF2, D-Mef2 | 606 | Annotation score: |
Sequence cautioni
The sequence AAL28644 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAM48340 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 3 | MGR → WAA in AAL28644 (PubMed:12537569).Curated | 3 | |
Sequence conflicti | 1 – 3 | MGR → WAA in AAM48340 (PubMed:12537569).Curated | 3 | |
Sequence conflicti | 97 | D → E in AAF06817 (PubMed:7839146).Curated | 1 | |
Sequence conflicti | 101 | A → G in AAF06817 (PubMed:7839146).Curated | 1 | |
Sequence conflicti | 112 – 114 | EAK → GGM in AAF06817 (PubMed:7839146).Curated | 3 | |
Sequence conflicti | 338 | S → R in AAA20463 (PubMed:8052612).Curated | 1 | |
Sequence conflicti | 364 – 377 | ASGHQ…GSTGS → PAVISRIAMVPRAG in AAA19957 (PubMed:8202544).CuratedAdd BLAST | 14 | |
Sequence conflicti | 364 – 377 | ASGHQ…GSTGS → PAVISRIAMVPRAG in AAF06817 (PubMed:7839146).CuratedAdd BLAST | 14 | |
Sequence conflicti | 429 | S → T in AAF06817 (PubMed:7839146).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041851 | 187 – 225 | Missing in isoform F. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_006237 | 187 – 193 | GGMSLII → V in isoform A and isoform B. 1 Publication | 7 | |
Alternative sequenceiVSP_006238 | 468 – 491 | GHDKY…WNLNF → DFIILN in isoform A. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_006239 | 469 – 493 | Missing in isoform B and isoform C. 4 PublicationsAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03292 mRNA Translation: AAA19957.1 U07422 mRNA Translation: AAA20463.1 X83527 mRNA Translation: CAA58508.1 U19493 Genomic DNA Translation: AAF06817.1 AE013599 Genomic DNA Translation: AAF58872.1 AE013599 Genomic DNA Translation: AAM71042.1 AE013599 Genomic DNA Translation: AAM71043.1 AE013599 Genomic DNA Translation: AAM71044.1 AE013599 Genomic DNA Translation: AAS64881.1 AY061096 mRNA Translation: AAL28644.1 Different initiation. AY061589 mRNA Translation: AAL29137.1 AY118311 mRNA Translation: AAM48340.1 Different initiation. |
RefSeqi | NP_477018.1, NM_057670.5 [P40791-4] NP_477019.1, NM_057671.5 [P40791-3] NP_477020.1, NM_057672.5 [P40791-1] NP_477021.1, NM_057673.5 [P40791-2] NP_995789.1, NM_206067.4 [P40791-6] |
Genome annotation databases
EnsemblMetazoai | FBtr0088443; FBpp0087529; FBgn0011656 [P40791-1] FBtr0088444; FBpp0087530; FBgn0011656 [P40791-2] FBtr0088445; FBpp0087531; FBgn0011656 [P40791-4] FBtr0088446; FBpp0087532; FBgn0011656 [P40791-3] FBtr0088447; FBpp0089303; FBgn0011656 [P40791-6] |
GeneIDi | 36032 |
KEGGi | dme:Dmel_CG1429 |
UCSCi | CG1429-RF, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03292 mRNA Translation: AAA19957.1 U07422 mRNA Translation: AAA20463.1 X83527 mRNA Translation: CAA58508.1 U19493 Genomic DNA Translation: AAF06817.1 AE013599 Genomic DNA Translation: AAF58872.1 AE013599 Genomic DNA Translation: AAM71042.1 AE013599 Genomic DNA Translation: AAM71043.1 AE013599 Genomic DNA Translation: AAM71044.1 AE013599 Genomic DNA Translation: AAS64881.1 AY061096 mRNA Translation: AAL28644.1 Different initiation. AY061589 mRNA Translation: AAL29137.1 AY118311 mRNA Translation: AAM48340.1 Different initiation. |
RefSeqi | NP_477018.1, NM_057670.5 [P40791-4] NP_477019.1, NM_057671.5 [P40791-3] NP_477020.1, NM_057672.5 [P40791-1] NP_477021.1, NM_057673.5 [P40791-2] NP_995789.1, NM_206067.4 [P40791-6] |
3D structure databases
SMRi | P40791 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 61860, 26 interactors |
IntActi | P40791, 3 interactors |
STRINGi | 7227.FBpp0087529 |
PTM databases
iPTMneti | P40791 |
Genome annotation databases
EnsemblMetazoai | FBtr0088443; FBpp0087529; FBgn0011656 [P40791-1] FBtr0088444; FBpp0087530; FBgn0011656 [P40791-2] FBtr0088445; FBpp0087531; FBgn0011656 [P40791-4] FBtr0088446; FBpp0087532; FBgn0011656 [P40791-3] FBtr0088447; FBpp0089303; FBgn0011656 [P40791-6] |
GeneIDi | 36032 |
KEGGi | dme:Dmel_CG1429 |
UCSCi | CG1429-RF, d. melanogaster |
Organism-specific databases
CTDi | 36032 |
FlyBasei | FBgn0011656, Mef2 |
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
GeneTreei | ENSGT00940000169350 |
InParanoidi | P40791 |
OMAi | LVMPNSR |
PhylomeDBi | P40791 |
Enzyme and pathway databases
Reactomei | R-DME-525793, Myogenesis |
SignaLinki | P40791 |
Miscellaneous databases
BioGRID-ORCSi | 36032, 0 hits in 3 CRISPR screens |
GenomeRNAii | 36032 |
PROi | PR:P40791 |
Gene expression databases
Bgeei | FBgn0011656, Expressed in spermathecum and 62 other tissues |
ExpressionAtlasi | P40791, differential |
Genevisiblei | P40791, DM |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR022102, HJURP_C IPR033896, MADS_MEF2-like IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF12347, HJURP_C, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MEF2_DROME | |
Accessioni | P40791Primary (citable) accession number: P40791 Secondary accession number(s): A1Z821 Q9U5I8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 19, 2011 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families