UniProtKB - P40749 (SYT4_MOUSE)
Protein
Synaptotagmin-4
Gene
Syt4
Organism
Mus musculus (Mouse)
Status
Functioni
Synaptotagmin family member which does not bind Ca2+. Plays a role in dendrite formation by melanocytes (By similarity).By similarity
Synaptotagmin family member which does not bind Ca2+ (By similarity). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses (PubMed:29166604). Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication
Cofactori
Ca2+PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 246 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 249 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 252 | CalciumPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium-dependent phospholipid binding Source: ParkinsonsUK-UCL
- calcium ion binding Source: MGI
- clathrin binding Source: MGI
- phosphatidylserine binding Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- SNARE binding Source: MGI
- syntaxin-1 binding Source: MGI
- syntaxin-3 binding Source: MGI
- syntaxin binding Source: MGI
GO - Biological processi
- brain development Source: Ensembl
- calcium-ion regulated exocytosis Source: GO_Central
- cellular response to calcium ion Source: GO_Central
- dense core granule cytoskeletal transport Source: UniProtKB
- memory Source: ParkinsonsUK-UCL
- negative regulation of calcium ion-dependent exocytosis Source: MGI
- negative regulation of catecholamine secretion Source: MGI
- negative regulation of dense core granule exocytosis Source: MGI
- negative regulation of neurotransmitter secretion Source: MGI
- negative regulation of protein secretion Source: SynGO-UCL
- negative regulation of retrograde trans-synaptic signaling by neuropeptide Source: SynGO-UCL
- negative regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
- negative regulation of synaptic vesicle exocytosis Source: SynGO-UCL
- negative regulation of vesicle fusion Source: MGI
- neurotransmitter secretion Source: MGI
- positive regulation of dendrite extension Source: MGI
- positive regulation of dense core granule exocytosis Source: ParkinsonsUK-UCL
- positive regulation of glutamate secretion Source: ParkinsonsUK-UCL
- regulation of calcium ion-dependent exocytosis Source: ParkinsonsUK-UCL
- regulation of dopamine secretion Source: ParkinsonsUK-UCL
- regulation of endocytosis Source: ParkinsonsUK-UCL
- regulation of presynaptic dense core granule exocytosis Source: SynGO
- regulation of vesicle fusion Source: ParkinsonsUK-UCL
- secretory granule maturation Source: MGI
- synaptic vesicle transport Source: UniProtKB
- trans-synaptic signaling by BDNF, modulating synaptic transmission Source: SynGO
- vesicle fusion Source: GO_Central
- vesicle fusion with vesicle Source: MGI
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | Differentiation |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Synaptotagmin-4Alternative name(s): Synaptotagmin IV Short name: SytIV |
Gene namesi | Name:Syt4 Synonyms:Syt3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101759, Syt4 |
Subcellular locationi
Other locations
- neuronal dense core vesicle membrane By similarity; Single-pass membrane protein By similarity
Extracellular region or secreted
- microvesicle Source: ParkinsonsUK-UCL
Golgi apparatus
- Golgi apparatus Source: ParkinsonsUK-UCL
Plasma Membrane
- plasma membrane Source: ParkinsonsUK-UCL
Other locations
- astrocyte projection Source: ParkinsonsUK-UCL
- axon Source: ParkinsonsUK-UCL
- dendrite Source: ParkinsonsUK-UCL
- dense core granule Source: ParkinsonsUK-UCL
- exocytic vesicle Source: MGI
- glutamatergic synapse Source: SynGO
- integral component of membrane Source: UniProtKB
- integral component of neuronal dense core vesicle membrane Source: SynGO
- intracellular membrane-bounded organelle Source: MGI
- intrinsic component of neuronal dense core vesicle membrane Source: UniProtKB
- neuron projection Source: MGI
- neuron projection terminus Source: MGI
- neuronal cell body Source: ParkinsonsUK-UCL
- neuronal dense core vesicle Source: UniProtKB
- perinuclear region of cytoplasm Source: ParkinsonsUK-UCL
- postsynapse Source: SynGO
- presynapse Source: SynGO-UCL
- secretory granule membrane Source: MGI
- somatodendritic compartment Source: ParkinsonsUK-UCL
- synapse Source: GO_Central
- vesicle Source: ParkinsonsUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | VesicularSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 425 | CytoplasmicSequence analysisAdd BLAST | 388 |
Keywords - Cellular componenti
Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000183949 | 1 – 425 | Synaptotagmin-4Add BLAST | 425 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 135 | Phosphoserine; by MAPK8Combined sources | 1 |
Post-translational modificationi
Phosphorylation at Ser-135 by MAPK8/JNK1 reduces interaction with KIF1A and neuronal dense core vesicles mobility.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P40749 |
PaxDbi | P40749 |
PRIDEi | P40749 |
PTM databases
iPTMneti | P40749 |
PhosphoSitePlusi | P40749 |
Expressioni
Tissue specificityi
Expressed in many regions of the nervous system but is undetectable in extra neural tissues (PubMed:8058779).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000024261, Expressed in pituitary gland and 190 other tissues |
Genevisiblei | P40749, MM |
Interactioni
Subunit structurei
Interacts with KIF1A; the interaction increases in presence of calcium and decreases when SYT4 is phosphorylated at Ser-135.
By similarityGO - Molecular functioni
- clathrin binding Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- SNARE binding Source: MGI
- syntaxin-1 binding Source: MGI
- syntaxin-3 binding Source: MGI
- syntaxin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203614, 3 interactors |
STRINGi | 10090.ENSMUSP00000025110 |
Miscellaneous databases
RNActi | P40749, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 153 – 274 | C2 1PROSITE-ProRule annotationAdd BLAST | 122 | |
Domaini | 287 – 420 | C2 2PROSITE-ProRule annotationAdd BLAST | 134 |
Domaini
Unlike in other synaptotagmin family members, the first C2 domain/C2A does not bind Ca2+ neither mediates Ca2+-dependent phospholipid binding. An aspartate-to-serine substitution in this domain inactivates Ca2+/phospho-lipid binding.By similarity
Sequence similaritiesi
Belongs to the synaptotagmin family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1028, Eukaryota |
GeneTreei | ENSGT00940000159026 |
HOGENOMi | CLU_023008_7_3_1 |
InParanoidi | P40749 |
OMAi | DFPRRQI |
OrthoDBi | 925064at2759 |
PhylomeDBi | P40749 |
TreeFami | TF315600 |
Family and domain databases
Gene3Di | 2.60.40.150, 2 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR001565, Synaptotagmin IPR028683, SYT4 |
PANTHERi | PTHR10024:SF114, PTHR10024:SF114, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 2 hits |
PRINTSi | PR00399, SYNAPTOTAGMN |
SMARTi | View protein in SMART SM00239, C2, 2 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 2 hits |
i Sequence
Sequence statusi: Complete.
P40749-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPITTSRVE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR RSAKSNKTPP
60 70 80 90 100
YKFVHVLKGV DIYPENLSSK KKFGGDDKSE VKGKAALPNL SLHLDLEKRD
110 120 130 140 150
LNGNFPKANP KAGSSSDLEN VTPKLFTETE KEANSPESLK SSTSLTSEEK
160 170 180 190 200
QEKLGTLFLS LEYNFEKKAF VVNIKEAQGL PAMDEQSMTS DPYIKMTILP
210 220 230 240 250
EKKHRVKTRV LRKTLDPVFD ETFTFYGIPY PHIQELSLHF TVLSFDRFSR
260 270 280 290 300
DDVIGEVLIP LSGIELSDGK MLMTREIIKR NAKKSSGRGE LLVSLCYQST
310 320 330 340 350
TNTLTVVVLK ARHLPKSDVS GLSDPYVKVN LYHAKKRISK KKTHVKKCTP
360 370 380 390 400
NAVFNELFVF DIPCESLEEI SVEFLVLDSE RGSRNEVIGR LVLGATAEGS
410 420
GGGHWKEICD FPRRQIAKWH MLCDG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | K → Q in AAA20971 (PubMed:8058779).Curated | 1 | |
Sequence conflicti | 85 | A → T in AAA20971 (PubMed:8058779).Curated | 1 | |
Sequence conflicti | 220 | D → G in AAA20971 (PubMed:8058779).Curated | 1 | |
Sequence conflicti | 246 | D → N in BAC31726 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10355 mRNA Translation: AAA20971.1 AK032106 mRNA Translation: BAC27705.1 AK043985 mRNA Translation: BAC31726.1 AK050515 mRNA Translation: BAC34301.1 AK083960 mRNA Translation: BAC39080.1 AK148681 mRNA Translation: BAE28640.1 BC058208 mRNA Translation: AAH58208.1 |
CCDSi | CCDS29109.1 |
RefSeqi | NP_033334.2, NM_009308.3 |
Genome annotation databases
Ensembli | ENSMUST00000025110; ENSMUSP00000025110; ENSMUSG00000024261 |
GeneIDi | 20983 |
KEGGi | mmu:20983 |
UCSCi | uc008ehy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10355 mRNA Translation: AAA20971.1 AK032106 mRNA Translation: BAC27705.1 AK043985 mRNA Translation: BAC31726.1 AK050515 mRNA Translation: BAC34301.1 AK083960 mRNA Translation: BAC39080.1 AK148681 mRNA Translation: BAE28640.1 BC058208 mRNA Translation: AAH58208.1 |
CCDSi | CCDS29109.1 |
RefSeqi | NP_033334.2, NM_009308.3 |
3D structure databases
SMRi | P40749 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203614, 3 interactors |
STRINGi | 10090.ENSMUSP00000025110 |
PTM databases
iPTMneti | P40749 |
PhosphoSitePlusi | P40749 |
Proteomic databases
MaxQBi | P40749 |
PaxDbi | P40749 |
PRIDEi | P40749 |
Protocols and materials databases
Antibodypediai | 22400, 348 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025110; ENSMUSP00000025110; ENSMUSG00000024261 |
GeneIDi | 20983 |
KEGGi | mmu:20983 |
UCSCi | uc008ehy.1, mouse |
Organism-specific databases
CTDi | 6860 |
MGIi | MGI:101759, Syt4 |
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota |
GeneTreei | ENSGT00940000159026 |
HOGENOMi | CLU_023008_7_3_1 |
InParanoidi | P40749 |
OMAi | DFPRRQI |
OrthoDBi | 925064at2759 |
PhylomeDBi | P40749 |
TreeFami | TF315600 |
Miscellaneous databases
BioGRID-ORCSi | 20983, 2 hits in 19 CRISPR screens |
ChiTaRSi | Syt3, mouse |
PROi | PR:P40749 |
RNActi | P40749, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024261, Expressed in pituitary gland and 190 other tissues |
Genevisiblei | P40749, MM |
Family and domain databases
Gene3Di | 2.60.40.150, 2 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR001565, Synaptotagmin IPR028683, SYT4 |
PANTHERi | PTHR10024:SF114, PTHR10024:SF114, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 2 hits |
PRINTSi | PR00399, SYNAPTOTAGMN |
SMARTi | View protein in SMART SM00239, C2, 2 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYT4_MOUSE | |
Accessioni | P40749Primary (citable) accession number: P40749 Secondary accession number(s): Q3UFC1, Q8BGH3, Q8BRL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 11, 2003 | |
Last modified: | February 10, 2021 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families