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Protein

Acetylornithine/succinyldiaminopimelate aminotransferase

Gene

argD

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in both the arginine and lysine biosynthetic pathways.UniRule annotation

Miscellaneous

The reaction catalyzed by ACOAT is highly reversible. This enzyme may also transaminate ornithine.1 Publication

Catalytic activityi

N2-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.UniRule annotation1 Publication
N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 PublicationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation1 Publication

Activity regulationi

Inhibited by gabaculine (Gcn).1 Publication

Kineticsi

  1. KM=37 µM for N-acetylornithine (at pH 9.5 and 25 degrees Celsius)1 Publication
  2. KM=640 µM for ornithine (at pH 9.5 and 25 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 9.5. At pH 8.0, the activity is reduced by 50%.1 Publication

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation1 Publication
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. Amino-acid acetyltransferase (argA)
    2. Acetylglutamate kinase (argB)
    3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
    4. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Pathwayi: L-lysine biosynthesis via DAP pathway

    This protein is involved in step 2 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).UniRule annotation
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
    2. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
    3. Succinyl-diaminopimelate desuccinylase (dapE)
    This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei141Pyridoxal phosphate; via carbonyl oxygenUniRule annotation1 Publication1
    Binding sitei144N2-acetyl-L-ornithineUniRule annotation1
    Binding sitei283N2-acetyl-L-ornithineUniRule annotation1
    Binding sitei284Pyridoxal phosphateUniRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAminotransferase, Transferase
    Biological processAmino-acid biosynthesis, Arginine biosynthesis, Lysine biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciSENT99287:G1FZD-3503-MONOMER
    SABIO-RKiP40732
    UniPathwayi
    UPA00034;UER00020

    UPA00068;UER00109

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetylornithine/succinyldiaminopimelate aminotransferaseUniRule annotationCurated (EC:2.6.1.11UniRule annotation1 Publication, EC:2.6.1.17UniRule annotation)
    Short name:
    ACOATUniRule annotation
    Short name:
    DapATaseUniRule annotation
    Short name:
    Succinyldiaminopimelate transferaseUniRule annotation
    Gene namesi
    Name:argDUniRule annotation
    Synonyms:dapC, dtu
    Ordered Locus Names:STM3468
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00001127772 – 405Acetylornithine/succinyldiaminopimelate aminotransferaseAdd BLAST404

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei255N6-(pyridoxal phosphate)lysineUniRule annotation1 Publication1

    Proteomic databases

    PaxDbiP40732
    PRIDEiP40732

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM3468

    Structurei

    Secondary structure

    1405
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP40732
    SMRiP40732
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40732

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni108 – 109Pyridoxal phosphate bindingUniRule annotation1 Publication2
    Regioni226 – 229Pyridoxal phosphate bindingUniRule annotation1 Publication4

    Sequence similaritiesi

    Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105C8Y Bacteria
    COG4992 LUCA
    HOGENOMiHOG000020206
    KOiK00821
    OMAiHSAWDLC
    PhylomeDBiP40732

    Family and domain databases

    CDDicd00610 OAT_like, 1 hit
    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    HAMAPiMF_01107 ArgD_aminotrans_3, 1 hit
    InterProiView protein in InterPro
    IPR017652 Ac/SucOrn_transaminase_bac
    IPR004636 AcOrn/SuccOrn_fam
    IPR005814 Aminotrans_3
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PfamiView protein in Pfam
    PF00202 Aminotran_3, 1 hit
    PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR03246 arg_catab_astC, 1 hit
    TIGR00707 argD, 1 hit
    PROSITEiView protein in PROSITE
    PS00600 AA_TRANSFER_CLASS_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40732-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MATEQTAITR ATFDEVILPV YAPADFIPVK GKGSRVWDQQ GKEYIDFAGG
    60 70 80 90 100
    IAVTALGHCH PALVEALKSQ GETLWHTSNV FTNEPALRLG RKLIDATFAE
    110 120 130 140 150
    RVLFMNSGTE ANETAFKLAR HYACVRHSPF KTKIIAFHNA FHGRSLFTVS
    160 170 180 190 200
    VGGQPKYSDG FGPKPADIIH VPFNDLHAVK AVMDDHTCAV VVEPIQGEGG
    210 220 230 240 250
    VQAATPEFLK GLRDLCDEHQ ALLVFDEVQC GMGRTGDLFA YMHYGVTPDI
    260 270 280 290 300
    LTSAKALGGG FPVSAMLTTQ EIASAFHVGS HGSTYGGNPL ACAVAGAAFD
    310 320 330 340 350
    IINTPEVLQG IHTKRQQFVQ HLQAIDEQFD IFSDIRGMGL LIGAELKPKY
    360 370 380 390 400
    KGRARDFLYA GAEAGVMVLN AGADVMRFAP SLVVEEADIH EGMQRFAQAV

    GKVVA
    Length:405
    Mass (Da):43,670
    Last modified:January 23, 2007 - v3
    Checksum:i26A69C62831E986C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti3T → I in AAA24265 (PubMed:2403545).Curated1
    Sequence conflicti3T → I in AAA27178 (PubMed:2403545).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA Translation: AAL22330.1
    M32354 Genomic DNA Translation: AAA24265.1
    M32355 Genomic DNA Translation: AAA27178.1
    RefSeqiNP_462371.1, NC_003197.2
    WP_000190023.1, NC_003197.2

    Genome annotation databases

    EnsemblBacteriaiAAL22330; AAL22330; STM3468
    GeneIDi1254991
    KEGGistm:STM3468
    PATRICifig|99287.12.peg.3665

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA Translation: AAL22330.1
    M32354 Genomic DNA Translation: AAA24265.1
    M32355 Genomic DNA Translation: AAA27178.1
    RefSeqiNP_462371.1, NC_003197.2
    WP_000190023.1, NC_003197.2

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2PB0X-ray1.96A/B1-405[»]
    2PB2X-ray1.91A/B1-405[»]
    4JEVX-ray1.67A/B1-405[»]
    4JEWX-ray1.48A/B1-405[»]
    4JEXX-ray1.43A/B1-405[»]
    4JEYX-ray1.55A/B1-405[»]
    4JEZX-ray1.55A/B1-405[»]
    4JF0X-ray2.10A/B1-405[»]
    4JF1X-ray1.28A/B1-405[»]
    ProteinModelPortaliP40732
    SMRiP40732
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM3468

    Proteomic databases

    PaxDbiP40732
    PRIDEiP40732

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL22330; AAL22330; STM3468
    GeneIDi1254991
    KEGGistm:STM3468
    PATRICifig|99287.12.peg.3665

    Phylogenomic databases

    eggNOGiENOG4105C8Y Bacteria
    COG4992 LUCA
    HOGENOMiHOG000020206
    KOiK00821
    OMAiHSAWDLC
    PhylomeDBiP40732

    Enzyme and pathway databases

    UniPathwayi
    UPA00034;UER00020

    UPA00068;UER00109

    BioCyciSENT99287:G1FZD-3503-MONOMER
    SABIO-RKiP40732

    Miscellaneous databases

    EvolutionaryTraceiP40732

    Family and domain databases

    CDDicd00610 OAT_like, 1 hit
    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    HAMAPiMF_01107 ArgD_aminotrans_3, 1 hit
    InterProiView protein in InterPro
    IPR017652 Ac/SucOrn_transaminase_bac
    IPR004636 AcOrn/SuccOrn_fam
    IPR005814 Aminotrans_3
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PfamiView protein in Pfam
    PF00202 Aminotran_3, 1 hit
    PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR03246 arg_catab_astC, 1 hit
    TIGR00707 argD, 1 hit
    PROSITEiView protein in PROSITE
    PS00600 AA_TRANSFER_CLASS_3, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiARGD_SALTY
    AccessioniPrimary (citable) accession number: P40732
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: November 7, 2018
    This is version 145 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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