UniProtKB - P40710 (NLPE_ECOLI)
Lipoprotein NlpE
nlpE
Functioni
Involved in copper homeostasis, could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes (PubMed:7635807).
Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation (PubMed:11830644).
Functions during envelope stress responses; when overproduced induces degP through the activation of the two-component envelope stress response system CpxA/CpxR (PubMed:7635808, PubMed:15252048).
DegP induction seems to require membrane anchoring of this protein (PubMed:15252048).
Structural changes and/or interaction of the CXXC motif with its environment may lead to activation of the Cpx stress response (PubMed:17698001).
4 PublicationsGO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- cell adhesion Source: UniProtKB-KW
- regulation of cell-substrate adhesion Source: EcoCyc
- stress response to copper ion Source: EcoCyc
Keywordsi
Biological process | Cell adhesion |
Ligand | Copper |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12137-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Lipoprotein NlpE1 PublicationAlternative name(s): Copper homeostasis protein CutF1 Publication |
Gene namesi | Name:nlpE1 Publication Synonyms:cutF1 Publication Ordered Locus Names:b0192, JW0188 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane 1 Publication1 Publication; Lipid-anchor 2 Publications
Other locations
- cell outer membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 236 | Periplasmic1 PublicationAdd BLAST | 216 |
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | C → A: No longer induces degP when overexpressed, targeted to the periplasm. 1 Publication | 1 | |
Mutagenesisi | 22 – 23 | NN → DD: Slightly stronger than normal induction of degP when overexpressed, mistargeted to inner membrane. 1 Publication | 2 | |
Mutagenesisi | 51 – 54 | CADC → SADS: Forms oxidized monomers. 1 Publication | 4 | |
Mutagenesisi | 51 | C → S: Forms oxidized monomers. 1 Publication | 1 | |
Mutagenesisi | 54 | C → S: Forms oxidized monomers. 1 Publication | 1 | |
Mutagenesisi | 165 | C → S: No oxidized monomer forms; when associated with Ser-231. 1 Publication | 1 | |
Mutagenesisi | 231 | C → S: No oxidized monomer forms; when associated with Ser-165. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Add BLAST | 20 | |
ChainiPRO_0000018036 | 21 – 236 | Lipoprotein NlpEAdd BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 21 | N-palmitoyl cysteinePROSITE-ProRule annotation1 Publication | 1 | |
Lipidationi | 21 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 165 ↔ 231 | 1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Lipoprotein, PalmitateProteomic databases
jPOSTi | P40710 |
PaxDbi | P40710 |
PRIDEi | P40710 |
Interactioni
Subunit structurei
Probably exists as a monomer in vivo, can however form homodimers which swap domains (PubMed:17698001).
1 PublicationBinary interactionsi
P40710
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-1116525,EBI-1116525 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260842, 209 interactors |
DIPi | DIP-9353N |
IntActi | P40710, 3 interactors |
STRINGi | 511145.b0192 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P40710 |
SMRi | P40710 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40710 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 100 | N-terminal domain1 PublicationAdd BLAST | 80 | |
Regioni | 126 – 236 | C-terminal domain1 PublicationAdd BLAST | 111 | |
Regioni | 144 – 156 | Could contain a copper-binding motif1 PublicationAdd BLAST | 13 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 51 – 54 | CXXC1 Publication | 4 |
Domaini
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3015, Bacteria |
HOGENOMi | CLU_1219320_0_0_6 |
InParanoidi | P40710 |
OMAi | GTWVMNQ |
Family and domain databases
Gene3Di | 2.40.128.300, 1 hit 2.40.50.540, 1 hit |
InterProi | View protein in InterPro IPR007298, Cu-R_lipoprotein_NlpE IPR033450, NlpE_C IPR038139, NlpE_C_sf IPR043176, NlpE_N_sf |
Pfami | View protein in Pfam PF04170, NlpE, 1 hit PF17185, NlpE_C, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVKKAIVTAM AVISLFTLMG CNNRAEVDTL SPAQAAELKP MPQSWRGVLP
60 70 80 90 100
CADCEGIETS LFLEKDGTWV MNERYLGARE EPSSFASYGT WARTADKLVL
110 120 130 140 150
TDSKGEKSYY RAKGDALEML DREGNPIESQ FNYTLEAAQS SLPMTPMTLR
160 170 180 190 200
GMYFYMADAA TFTDCATGKR FMVANNAELE RSYLAARGHS EKPVLLSVEG
210 220 230
HFTLEGNPDT GAPTKVLAPD TAGKFYPNQD CSSLGQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18345 Genomic DNA Translation: AAA86093.1 L38619 Genomic DNA Translation: AAA82972.1 D49445 Genomic DNA Translation: BAA08433.1 U70214 Genomic DNA Translation: AAB08620.1 U00096 Genomic DNA Translation: AAC73303.1 AP009048 Genomic DNA Translation: BAA77868.1 |
PIRi | H64743 |
RefSeqi | NP_414734.1, NC_000913.3 WP_000239163.1, NZ_SSZK01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC73303; AAC73303; b0192 BAA77868; BAA77868; BAA77868 |
GeneIDi | 946782 |
KEGGi | ecj:JW0188 eco:b0192 |
PATRICi | fig|1411691.4.peg.2086 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18345 Genomic DNA Translation: AAA86093.1 L38619 Genomic DNA Translation: AAA82972.1 D49445 Genomic DNA Translation: BAA08433.1 U70214 Genomic DNA Translation: AAB08620.1 U00096 Genomic DNA Translation: AAC73303.1 AP009048 Genomic DNA Translation: BAA77868.1 |
PIRi | H64743 |
RefSeqi | NP_414734.1, NC_000913.3 WP_000239163.1, NZ_SSZK01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Z4H | X-ray | 2.80 | A/B | 21-236 | [»] | |
2Z4I | X-ray | 2.60 | A/B | 21-236 | [»] | |
AlphaFoldDBi | P40710 | |||||
SMRi | P40710 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260842, 209 interactors |
DIPi | DIP-9353N |
IntActi | P40710, 3 interactors |
STRINGi | 511145.b0192 |
Proteomic databases
jPOSTi | P40710 |
PaxDbi | P40710 |
PRIDEi | P40710 |
Genome annotation databases
EnsemblBacteriai | AAC73303; AAC73303; b0192 BAA77868; BAA77868; BAA77868 |
GeneIDi | 946782 |
KEGGi | ecj:JW0188 eco:b0192 |
PATRICi | fig|1411691.4.peg.2086 |
Organism-specific databases
EchoBASEi | EB2058 |
Phylogenomic databases
eggNOGi | COG3015, Bacteria |
HOGENOMi | CLU_1219320_0_0_6 |
InParanoidi | P40710 |
OMAi | GTWVMNQ |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12137-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P40710 |
PROi | PR:P40710 |
Family and domain databases
Gene3Di | 2.40.128.300, 1 hit 2.40.50.540, 1 hit |
InterProi | View protein in InterPro IPR007298, Cu-R_lipoprotein_NlpE IPR033450, NlpE_C IPR038139, NlpE_C_sf IPR043176, NlpE_N_sf |
Pfami | View protein in Pfam PF04170, NlpE, 1 hit PF17185, NlpE_C, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NLPE_ECOLI | |
Accessioni | P40710Primary (citable) accession number: P40710 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references