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Protein

DNA-binding protein SMUBP-2

Gene

Ighmbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver-type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region (By similarity). May be involved in translation. DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr.By similarity2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi213 – 220ATPBy similarity8
Zinc fingeri892 – 938AN1-typeAdd BLAST47

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: MGI
  • neuromuscular process Source: MGI
  • protein homooligomerization Source: MGI
  • spinal cord motor neuron differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • translation Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding, Helicase, Hydrolase, Ribonucleoprotein, RNA-binding, tRNA-binding
Biological processTranscription, Transcription regulation
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein SMUBP-2 (EC:3.6.4.12, EC:3.6.4.13)
Alternative name(s):
ATP-dependent helicase IGHMBP2
Cardiac transcription factor 1
Short name:
CATF1
Immunoglobulin mu-binding protein 2
Gene namesi
Name:Ighmbp2
Synonyms:Smbp-2, Smbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99954 Ighmbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000807032 – 993DNA-binding protein SMUBP-2Add BLAST992

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei797PhosphoserineCombined sources1
Modified residuei800PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP40694
MaxQBiP40694
PaxDbiP40694
PeptideAtlasiP40694
PRIDEiP40694

PTM databases

iPTMnetiP40694
PhosphoSitePlusiP40694

Expressioni

Tissue specificityi

In all tissues examined.

Gene expression databases

CleanExiMM_IGHMBP2

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with RUVBL1, RUVBL2, GTF3C1 and ABT1 (By similarity). Is part of large cytosolic ribonucleoprotein complexes (Probable). Associates with the ribosomes.By similarityCurated1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP40694, 1 interactor
STRINGi10090.ENSMUSP00000025751

Structurei

3D structure databases

ProteinModelPortaliP40694
SMRiP40694
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini721 – 784R3HPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni637 – 783SS DNA-bindingBy similarityAdd BLAST147

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi862 – 866Nuclear localization signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi249 – 425Leu-richAdd BLAST177
Compositional biasi370 – 373Poly-Val4
Compositional biasi793 – 861Gln/Pro-richAdd BLAST69
Compositional biasi862 – 866Poly-Lys5

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri892 – 938AN1-typeAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1803 Eukaryota
COG1112 LUCA
HOGENOMiHOG000185831
HOVERGENiHBG077019
InParanoidiP40694
PhylomeDBiP40694

Family and domain databases

CDDicd02641 R3H_Smubp-2_like, 1 hit
Gene3Di3.30.1370.50, 1 hit
4.10.1110.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR035896 AN1-like_Znf
IPR014001 Helicase_ATP-bd
IPR027417 P-loop_NTPase
IPR001374 R3H_dom
IPR036867 R3H_dom_sf
IPR034072 R3H_Smubp-2
IPR004483 SMUBP-2/Hcs1-like
IPR000058 Znf_AN1
PfamiView protein in Pfam
PF01424 R3H, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00487 DEXDc, 1 hit
SM00393 R3H, 1 hit
SM00154 ZnF_AN1, 1 hit
SUPFAMiSSF118310 SSF118310, 1 hit
SSF52540 SSF52540, 2 hits
SSF82708 SSF82708, 1 hit
TIGRFAMsiTIGR00376 TIGR00376, 1 hit
PROSITEiView protein in PROSITE
PS51061 R3H, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSTVESFV AQQLQLLELE RDAEVEERRS WQEHSSLREL QSRGVCLLKL
60 70 80 90 100
QVSSQRTGLY GQRLVTFEPR KFGPAVVLPS NSFTSGDIVG LYDTNENSQL
110 120 130 140 150
ATGVLTRITQ KSVTVAFDES HDLQLNLDRE NTYRLLKLAN DVTYKRLKKA
160 170 180 190 200
LMTLKKYHSG PASSLIDILL GSSTPSPAME IPPLSFYNTT LDLSQKEAVS
210 220 230 240 250
FALAQKELAI IHGPPGTGKT TTVVEIILQA VKQGLKVLCC APSNIAVDNL
260 270 280 290 300
VERLALCKKR ILRLGHPARL LESVQHHSLD AVLARSDNAQ IVADIRRDID
310 320 330 340 350
QVFGKNKKTQ DKREKGNFRS EIKLLRKELK EREEAAIVQS LTAADVVLAT
360 370 380 390 400
NTGASSDGPL KLLPEDYFDV VVVDECAQAL EASCWIPLLK APKCILAGDH
410 420 430 440 450
RQLPPTTVSH RAALAGLSRS LMERLAEKHG AGVVRMLTVQ YRMHQAIMCW
460 470 480 490 500
ASEAMYHGQF TSHPSVAGHL LKDLPGVTDT EETRVPLLLI DTAGCGLLEL
510 520 530 540 550
EEEDSQSKGN PGEVRLVTLH IQALVDAGVQ AGDIAVIAPY NLQVDLLRQS
560 570 580 590 600
LSNKHPELEI KSVDGFQGRE KEAVLLTFVR SNRKGEVGFL AEDRRINVAV
610 620 630 640 650
TRARRHVAVI CDSHTVNNHA FLETLVDYFT EHGEVRTAFE YLDDIVPENY
660 670 680 690 700
THEGSQGHSR VPKPKCPSTS IRKPASDQES GQETRAAPRH GRRKPSEKPP
710 720 730 740 750
GSHVQSQHSS SANGSDRTGG PDRTEHFRAT IEEFVASKES QLEFPTSLSS
760 770 780 790 800
HDRLRVHQLA EEFGLRHDST GEGKARHITV SRRSPASSGS VAPQPSSPPS
810 820 830 840 850
PAQAEPEPRA EEPVTVVQAH CPVQLDLKAL HLERLQRQQS SQAQTAKGQP
860 870 880 890 900
GGDSRPQKAS QKKKKKEPKG PVMALPCEED FDALVSAVVK ADNTCSFSKC
910 920 930 940 950
SVSTTTLGQF CMHCSHRYYL SHHLPEIHGC GEKARAHARQ RISREGVLYA
960 970 980 990
GSGTKDRALD PAKRAQLQRR LDKKLGELSS QRTSRKKEKE RGT
Length:993
Mass (Da):109,466
Last modified:February 1, 1995 - v1
Checksum:i2FA0850DBABDE35B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10075 mRNA Translation: AAA40143.1
CCDSiCCDS29393.1
PIRiS35633
UniGeneiMm.3179

Genome annotation databases

UCSCiuc008fwa.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSMBP2_MOUSE
AccessioniPrimary (citable) accession number: P40694
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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