UniProtKB - P40645 (SOX6_MOUSE)
Protein
Transcription factor SOX-6
Gene
Sox6
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that plays a key role in several developmental processes, including neurogenesis, chondrocytes differentiation and cartilage formation (PubMed:7567444, PubMed:11702786, PubMed:15529345, PubMed:26525805). Specifically binds the 5'-AACAAT-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis (PubMed:9755172, PubMed:11702786). Required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes: SOX5 and SOX6 cooperatively bind with SOX9 on active enhancers and super-enhancers associated with cartilage-specific genes, and thereby potentiate SOX9's ability to transactivate (PubMed:11702786, PubMed:15529345, PubMed:26150426). Not involved in precartilaginous condensation, the first step in chondrogenesis, during which skeletal progenitors differentiate into prechondrocytes (PubMed:14993235, PubMed:26150426). Together with SOX5, required to form and maintain a pool of highly proliferating chondroblasts between epiphyses and metaphyses, to form columnar chondroblasts, delay chondrocyte prehypertrophy but promote hypertrophy, and to delay terminal differentiation of chondrocytes on contact with ossification fronts (PubMed:14993235). Binds to the proximal promoter region of the myelin protein MPZ gene, and is thereby involved in the differentiation of oligodendroglia in the developing spinal tube (PubMed:26525805). Binds to the gene promoter of MBP and acts as a transcriptional repressor (PubMed:26525805).7 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 620 – 688 | HMG boxPROSITE-ProRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription repressor activity Source: UniProtKB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- astrocyte differentiation Source: MGI
- cardiac muscle cell differentiation Source: MGI
- cartilage condensation Source: UniProtKB
- cartilage development Source: UniProtKB
- cell fate commitment Source: MGI
- cell morphogenesis Source: MGI
- cellular response to transforming growth factor beta stimulus Source: MGI
- central nervous system development Source: GO_Central
- chondrocyte differentiation Source: UniProtKB
- erythrocyte development Source: MGI
- erythrocyte differentiation Source: MGI
- gene silencing Source: MGI
- hemopoiesis Source: MGI
- in utero embryonic development Source: MGI
- muscle cell differentiation Source: MGI
- negative regulation of cardiac muscle cell differentiation Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- oligodendrocyte cell fate specification Source: MGI
- oligodendrocyte differentiation Source: MGI
- positive regulation of cartilage development Source: MGI
- positive regulation of chondrocyte differentiation Source: UniProtKB
- positive regulation of mesenchymal stem cell differentiation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- post-embryonic development Source: MGI
- regulation of gene expression Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- spinal cord oligodendrocyte cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-3769402, Deactivation of the beta-catenin transactivating complex |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor SOX-6CuratedAlternative name(s): SOX-LZ1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98368, Sox6 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Newborn mice display mild skeletal abnormalities (PubMed:11702786). Knockout mice also display increase in prematurely differentiated oligodendrocyte precursor cells in the motor neuron progenitor domain of the spinal tube at 11.5 dpc (PubMed:26525805). At 18.5 dpc the number and distribution of oligodendroglia are normal, however there is an increased number of differentiating oligodendrocytes (PubMed:26525805). Double knockout of Sox13 and Sox6 show an increase in oligodendrocyte precursor cells in the spinal tube at 11.5 dpc, at 18.5 dpc the number and distribution of oligodendroglia are normal but contain an increased number of differentiating cells (PubMed:26525805). Mice lacking both Sox5 and Sox6 develop a severe chondrodysplasia characterized by the virtual absence of cartilage: chondrogenic cells are largely arrested at the stage of chondrogenic mesenchymal condensations (PubMed:11702786). Embryos lacking Sox5 (homozygous knockout) and heterozygous for Sox6 live until birth and show severe growth plate chondrocyte defects (PubMed:14993235). Embryos lacking Sox6 (homozygous knockout) and heterozygous for Sox5 live until birth and show severe growth plate chondrocyte defects (PubMed:14993235).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048730 | 1 – 827 | Transcription factor SOX-6Add BLAST | 827 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 119 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 399 | PhosphoserineCombined sources | 1 | |
Modified residuei | 401 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 404 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 417 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 439 | PhosphoserineCombined sources | 1 | |
Modified residuei | 442 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Sumoylation inhibits the transcriptional activity.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P40645 |
PaxDbi | P40645 |
PRIDEi | P40645 |
PTM databases
iPTMneti | P40645 |
PhosphoSitePlusi | P40645 |
Expressioni
Tissue specificityi
Highly expressed in testis.
Developmental stagei
Expression is transiently increased during brown adipocyte differentiation.1 Publication
Inductioni
Expression is dependent on SOX9.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000051910, Expressed in gastrocnemius medialis and 287 other tissues |
Genevisiblei | P40645, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 203410, 6 interactors |
IntActi | P40645, 1 interactor |
STRINGi | 10090.ENSMUSP00000072583 |
Miscellaneous databases
RNActi | P40645, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 184 – 262 | Sequence analysisAdd BLAST | 79 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 233 – 261 | Gln-richAdd BLAST | 29 | |
Compositional biasi | 240 – 243 | Poly-Gln | 4 | |
Compositional biasi | 280 – 285 | Poly-Ala | 6 | |
Compositional biasi | 313 – 317 | Poly-Ala | 5 | |
Compositional biasi | 514 – 517 | Poly-Gln | 4 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0528, Eukaryota |
GeneTreei | ENSGT00940000156433 |
InParanoidi | P40645 |
PhylomeDBi | P40645 |
TreeFami | TF320471 |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR027153, SOX-6 |
PANTHERi | PTHR45789:SF1, PTHR45789:SF1, 1 hit |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P40645-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSKQATSPF ACTADGEEAM TQDLTSREKE EGSDQHPASH LPLHPIMHNK
60 70 80 90 100
PHSEELPTLV STIQQDADWD SVLSSQQRME SENNKLCSLY SFRNTSTSPH
110 120 130 140 150
KPDEGSRERE IMNSVTFGTP ERRKGSLADV VDTLKQKKLE EMTRTEQEDS
160 170 180 190 200
SCMEKLLSKD WKEKMERLNT SELLGEIKGT PESLAEKERQ LSTMITQLIS
210 220 230 240 250
LREQLLAAHD EQKKLAASQI EKQRQQMDLA RQQQEQIARQ QQQLLQQQHK
260 270 280 290 300
INLLQQQIQV QGHMPPLMIP IFPHDQRTLA AAAAAQQGFL FPPGITYKPG
310 320 330 340 350
DNYPVQFIPS TMAAAAASGL SPLQLQKGHV SHPQINPRLK GISDRFGRNL
360 370 380 390 400
DPSEHGGGHS YNHRQIEQLY AAQLASMQVS PGAKMPSTPQ PPNSAGAVSP
410 420 430 440 450
TGIKNEKRGT SPVTQVKDET TAQPLNLSSR PKTAEPVKSP TSPTQNLFPA
460 470 480 490 500
SKTSPVNLPN KSSIPSPIGG SLGRGSSLDI LSSLNSPALF GDQDTVMKAI
510 520 530 540 550
QEARKMREQI QREQQQQPHG VDGKLSSMNN MGLSNCRTEK ERTRFENLGP
560 570 580 590 600
QLTGKSSEDG KLGPGVIDLT RPEDAEGSKA MNGSAAKLQQ YYCWPTGGAT
610 620 630 640 650
VAEARVYRDA RGRASSEPHI KRPMNAFMVW AKDERRKILQ AFPDMHNSNI
660 670 680 690 700
SKILGSRWKS MSNQEKQPYY EEQARLSKIH LEKYPNYKYK PRPKRTCIVD
710 720 730 740 750
GKKLRIGEYK QLMRSRRQEM RQFFTVGQQP QMPITTGTGV VYPGAITMAT
760 770 780 790 800
TTPSPQMTSD CSSTSASPEP SLPVIQSTYG MKMDGASLAG NDMINGEDEM
810 820
EAYDDYEDDP KSDYSSENEA PEPVSAN
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PUW0 | E9PUW0_MOUSE | Transcription factor SOX-6 | Sox6 | 787 | Annotation score: | ||
Q8BSS6 | Q8BSS6_MOUSE | Transcription factor SOX-6 | Sox6 | 785 | Annotation score: | ||
A0A0U1RNW8 | A0A0U1RNW8_MOUSE | Transcription factor SOX-6 | Sox6 | 828 | Annotation score: | ||
A0A0U1RPC1 | A0A0U1RPC1_MOUSE | Transcription factor SOX-6 | Sox6 | 786 | Annotation score: | ||
A0A0U1RNI3 | A0A0U1RNI3_MOUSE | Transcription factor SOX-6 | Sox6 | 125 | Annotation score: | ||
A0A0U1RNH0 | A0A0U1RNH0_MOUSE | Transcription factor SOX-6 | Sox6 | 111 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 312 – 313 | MA → SS in BAA09618 (PubMed:7791783).Curated | 2 | |
Sequence conflicti | 632 | K → R in CAA46610 (PubMed:1614875).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002198 | 327 – 367 | Missing in isoform 2. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32614 mRNA Translation: AAC52263.1 D61689 mRNA Translation: BAA09618.1 AJ010605 mRNA Translation: CAA09270.1 X65659 mRNA Translation: CAA46610.1 |
CCDSi | CCDS40098.1 [P40645-1] |
PIRi | S22944 S59121 |
RefSeqi | NP_001264255.1, NM_001277326.1 [P40645-1] NP_001264257.1, NM_001277328.1 [P40645-2] NP_035575.1, NM_011445.4 [P40645-1] XP_011240009.1, XM_011241707.2 [P40645-1] XP_011240013.1, XM_011241711.2 |
Genome annotation databases
Ensembli | ENSMUST00000072804; ENSMUSP00000072583; ENSMUSG00000051910 [P40645-1] ENSMUST00000166207; ENSMUSP00000129027; ENSMUSG00000051910 [P40645-1] |
GeneIDi | 20679 |
KEGGi | mmu:20679 |
UCSCi | uc009jin.2, mouse [P40645-2] uc009jip.2, mouse [P40645-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32614 mRNA Translation: AAC52263.1 D61689 mRNA Translation: BAA09618.1 AJ010605 mRNA Translation: CAA09270.1 X65659 mRNA Translation: CAA46610.1 |
CCDSi | CCDS40098.1 [P40645-1] |
PIRi | S22944 S59121 |
RefSeqi | NP_001264255.1, NM_001277326.1 [P40645-1] NP_001264257.1, NM_001277328.1 [P40645-2] NP_035575.1, NM_011445.4 [P40645-1] XP_011240009.1, XM_011241707.2 [P40645-1] XP_011240013.1, XM_011241711.2 |
3D structure databases
SMRi | P40645 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203410, 6 interactors |
IntActi | P40645, 1 interactor |
STRINGi | 10090.ENSMUSP00000072583 |
PTM databases
iPTMneti | P40645 |
PhosphoSitePlusi | P40645 |
Proteomic databases
jPOSTi | P40645 |
PaxDbi | P40645 |
PRIDEi | P40645 |
Protocols and materials databases
Antibodypediai | 1097, 269 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000072804; ENSMUSP00000072583; ENSMUSG00000051910 [P40645-1] ENSMUST00000166207; ENSMUSP00000129027; ENSMUSG00000051910 [P40645-1] |
GeneIDi | 20679 |
KEGGi | mmu:20679 |
UCSCi | uc009jin.2, mouse [P40645-2] uc009jip.2, mouse [P40645-1] |
Organism-specific databases
CTDi | 55553 |
MGIi | MGI:98368, Sox6 |
Phylogenomic databases
eggNOGi | KOG0528, Eukaryota |
GeneTreei | ENSGT00940000156433 |
InParanoidi | P40645 |
PhylomeDBi | P40645 |
TreeFami | TF320471 |
Enzyme and pathway databases
Reactomei | R-MMU-3769402, Deactivation of the beta-catenin transactivating complex |
Miscellaneous databases
BioGRID-ORCSi | 20679, 1 hit in 17 CRISPR screens |
ChiTaRSi | Sox6, mouse |
PROi | PR:P40645 |
RNActi | P40645, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000051910, Expressed in gastrocnemius medialis and 287 other tissues |
Genevisiblei | P40645, MM |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR027153, SOX-6 |
PANTHERi | PTHR45789:SF1, PTHR45789:SF1, 1 hit |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SOX6_MOUSE | |
Accessioni | P40645Primary (citable) accession number: P40645 Secondary accession number(s): Q62250, Q9QWS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot