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Protein

ADP-ribosylation factor-like protein 1

Gene

ARL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Magnesium1 Publication1
Metal bindingi48Magnesium1 Publication1
Binding sitei70GTP; via amide nitrogen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTP1 Publication8
Nucleotide bindingi45 – 48GTP1 Publication4
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTP1 Publication4
Nucleotide bindingi160 – 161GTP1 Publication2

GO - Molecular functioni

  • enzyme activator activity Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • activation of phospholipase D activity Source: UniProtKB
  • Golgi organization Source: UniProtKB
  • Golgi vesicle transport Source: Ensembl
  • protein localization to Golgi apparatus Source: UniProtKB
  • retrograde transport, endosome to Golgi Source: UniProtKB
  • toxin metabolic process Source: UniProtKB

Keywordsi

LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 1
Gene namesi
Name:ARL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000120805.13
HGNCiHGNC:692 ARL1
MIMi603425 gene
neXtProtiNX_P40616

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71Q → L: Altered Golgi structure with an engorged lumen. Interacts with ARFIP2, GOLGA4, RGPD8, SCOC and UNC119. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000120805
PharmGKBiPA24985

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiARL1
DMDMi728888

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002074502 – 181ADP-ribosylation factor-like protein 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP40616
MaxQBiP40616
PaxDbiP40616
PeptideAtlasiP40616
PRIDEiP40616
ProteomicsDBi55375
TopDownProteomicsiP40616-1 [P40616-1]

PTM databases

iPTMnetiP40616
PhosphoSitePlusiP40616
SwissPalmiP40616

Expressioni

Tissue specificityi

Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000120805
CleanExiHS_ARL1
ExpressionAtlasiP40616 baseline and differential
GenevisibleiP40616 HS

Organism-specific databases

HPAiHPA062656
HPA073012

Interactioni

Subunit structurei

The GTP-bound form interacts with GOLGA1 (By similarity). The GTP-bound form interacts with GOLGA4 and RGPD8. The GTP-bound form directly interacts with ARFIP2; this interaction leads to a > 4-fold increase in the amount of bound GTP at steady state level. Binds to SCOC, preferentially in its GTP-bound form. May interact with UNC119.By similarity2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi106893, 22 interactors
IntActiP40616, 26 interactors
MINTiP40616
STRINGi9606.ENSP00000261636

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 23Combined sources6
Helixi30 – 39Combined sources10
Beta strandi49 – 58Combined sources10
Beta strandi61 – 68Combined sources8
Helixi72 – 81Combined sources10
Beta strandi86 – 93Combined sources8
Turni97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 143Combined sources8
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 162Combined sources3
Helixi166 – 178Combined sources13

3D structure databases

ProteinModelPortaliP40616
SMRiP40616
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40616

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0072 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00920000149001
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP40616
KOiK07942
PhylomeDBiP40616
TreeFamiTF105461

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P40616-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG
60 70 80 90 100
FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI
110 120 130 140 150
GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD
160 170 180
RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q
Length:181
Mass (Da):20,418
Last modified:February 1, 1995 - v1
Checksum:i9FB736C8ED60DC3D
GO
Isoform 2 (identifier: P40616-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: No experimental confirmation available.
Show »
Length:164
Mass (Da):18,565
Checksum:i3B0DD968A4891E31
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0565661 – 17Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28997 mRNA Translation: AAC37567.1
AF493887 mRNA Translation: AAM12601.1
BT007260 mRNA Translation: AAP35924.1
AK301701 mRNA Translation: BAG63173.1
AK311793 mRNA Translation: BAG34736.1
BX537387 mRNA Translation: CAD97629.1
AC063948 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW97658.1
BC007000 mRNA Translation: AAH07000.1
CCDSiCCDS44958.1 [P40616-1]
RefSeqiNP_001168.1, NM_001177.5 [P40616-1]
UniGeneiHs.372616
Hs.732900

Genome annotation databases

EnsembliENST00000261636; ENSP00000261636; ENSG00000120805 [P40616-1]
ENST00000536227; ENSP00000441808; ENSG00000120805 [P40616-2]
ENST00000551828; ENSP00000448850; ENSG00000120805 [P40616-2]
GeneIDi400
KEGGihsa:400
UCSCiuc001tib.4 human [P40616-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARL1_HUMAN
AccessioniPrimary (citable) accession number: P40616
Secondary accession number(s): B4DWW1, P80417, Q53XB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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