UniProtKB - P40616 (ARL1_HUMAN)
ADP-ribosylation factor-like protein 1
ARL1
Functioni
GTP-binding protein that recruits several effectors, such as golgins, arfaptins and Arf-GEFs to the trans-Golgi network, and modulates their functions at the Golgi complex (PubMed:9624189, PubMed:21239483, PubMed:27436755, PubMed:22679020, PubMed:27373159).
Plays thereby a role in a wide range of fundamental cellular processes, including cell polarity, innate immunity, or protein secretion mediated by arfaptins, which were shown to play a role in maintaining insulin secretion from pancreatic beta cells (PubMed:22981988).
6 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 31 | Magnesium1 Publication | 1 | |
Metal bindingi | 48 | Magnesium1 Publication | 1 | |
Binding sitei | 70 | GTP; via amide nitrogen3 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 24 – 31 | GTP3 Publications | 8 | |
Nucleotide bindingi | 45 – 48 | GTP3 Publications | 4 | |
Nucleotide bindingi | 67 – 71 | GTPBy similarity | 5 | |
Nucleotide bindingi | 126 – 129 | GTP3 Publications | 4 | |
Nucleotide bindingi | 160 – 161 | GTP3 Publications | 2 |
GO - Molecular functioni
- enzyme activator activity Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein domain specific binding Source: UniProtKB
GO - Biological processi
- activation of phospholipase D activity Source: UniProtKB
- Golgi organization Source: UniProtKB
- Golgi vesicle transport Source: Ensembl
- intracellular protein transport Source: GO_Central
- protein localization to Golgi apparatus Source: UniProtKB
- retrograde transport, endosome to Golgi Source: UniProtKB
- toxin metabolic process Source: UniProtKB
- vesicle-mediated transport Source: GO_Central
Keywordsi
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P40616 |
Reactomei | R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network |
SignaLinki | P40616 |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor-like protein 1 |
Gene namesi | Name:ARL1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:692, ARL1 |
MIMi | 603425, gene |
neXtProti | NX_P40616 |
VEuPathDBi | HostDB:ENSG00000120805 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
- trans-Golgi network membrane 3 Publications
Other locations
- Membrane Curated; Lipid-anchor Curated
Cytosol
- cytosol Source: Reactome
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
- trans-Golgi network Source: CACAO
- trans-Golgi network membrane Source: Reactome
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | T → N: Loss of interaction with ARFIP1 and ARFIP2. 1 Publication | 1 | |
Mutagenesisi | 71 | Q → L: Altered Golgi structure with an engorged lumen. Interacts with ARFIP2, GOLGA4, RGPD8, SCOC and UNC119. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 400 |
OpenTargetsi | ENSG00000120805 |
PharmGKBi | PA24985 |
Miscellaneous databases
Pharosi | P40616, Tbio |
Chemistry databases
DrugBanki | DB04315, Guanosine-5'-Diphosphate |
Genetic variation databases
BioMutai | ARL1 |
DMDMi | 728888 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000207450 | 2 – 181 | ADP-ribosylation factor-like protein 1Add BLAST | 180 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, MyristateProteomic databases
EPDi | P40616 |
jPOSTi | P40616 |
MassIVEi | P40616 |
MaxQBi | P40616 |
PaxDbi | P40616 |
PeptideAtlasi | P40616 |
PRIDEi | P40616 |
ProteomicsDBi | 5387 55375 [P40616-1] |
TopDownProteomicsi | P40616-1 [P40616-1] |
PTM databases
iPTMneti | P40616 |
MetOSitei | P40616 |
PhosphoSitePlusi | P40616 |
SwissPalmi | P40616 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000120805, Expressed in islet of Langerhans and 252 other tissues |
ExpressionAtlasi | P40616, baseline and differential |
Genevisiblei | P40616, HS |
Organism-specific databases
HPAi | ENSG00000120805, Low tissue specificity |
Interactioni
Subunit structurei
The GTP-bound form interacts with GOLGA1 (By similarity). The GTP-bound form interacts with GOLGA4 and RGPD8. The GTP-bound form directly interacts with ARFIP2 (PubMed:11303027, PubMed:21239483, PubMed:22679020). Binds to SCOC, preferentially in its GTP-bound form. May interact with UNC119 (PubMed:11303027, PubMed:14580338).
Interacts with ARFIP1; this interaction directs ARFIP1 to the trans-Golgi membranes (PubMed:21239483, PubMed:22981988).
Interacts with ARFGEF1 (via N-terminus) (PubMed:27436755, PubMed:27373159).
By similarity7 PublicationsBinary interactionsi
P40616
With | #Exp. | IntAct |
---|---|---|
ARFIP1 [P53367] | 2 | EBI-1052746,EBI-2808808 |
GO - Molecular functioni
- protein domain specific binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 106893, 79 interactors |
CORUMi | P40616 |
IntActi | P40616, 36 interactors |
MINTi | P40616 |
STRINGi | 9606.ENSP00000261636 |
Miscellaneous databases
RNActi | P40616, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P40616 |
SMRi | P40616 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40616 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0072, Eukaryota |
GeneTreei | ENSGT00940000155118 |
HOGENOMi | CLU_040729_9_4_1 |
InParanoidi | P40616 |
OMAi | QEGMDWL |
OrthoDBi | 1271528at2759 |
PhylomeDBi | P40616 |
TreeFami | TF105461 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR024156, Small_GTPase_ARF IPR006689, Small_GTPase_ARF/SAR |
PANTHERi | PTHR11711, PTHR11711, 1 hit |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SMARTi | View protein in SMART SM00178, SAR, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG
60 70 80 90 100
FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI
110 120 130 140 150
GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD
160 170 180
RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB4DZG7 | B4DZG7_HUMAN | ADP-ribosylation factor-like protei... | ARL1 | 135 | Annotation score: | ||
F8W1Z8 | F8W1Z8_HUMAN | ADP-ribosylation factor-like protei... | ARL1 | 52 | Annotation score: | ||
F8VYN9 | F8VYN9_HUMAN | ADP-ribosylation factor-like protei... | ARL1 | 194 | Annotation score: | ||
F8VP63 | F8VP63_HUMAN | ADP-ribosylation factor-like protei... | ARL1 | 64 | Annotation score: | ||
F8VP99 | F8VP99_HUMAN | ADP-ribosylation factor-like protei... | ARL1 | 17 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056566 | 1 – 17 | Missing in isoform 2. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28997 mRNA Translation: AAC37567.1 AF493887 mRNA Translation: AAM12601.1 BT007260 mRNA Translation: AAP35924.1 AK301701 mRNA Translation: BAG63173.1 AK311793 mRNA Translation: BAG34736.1 BX537387 mRNA Translation: CAD97629.1 AC063948 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97658.1 BC007000 mRNA Translation: AAH07000.1 |
CCDSi | CCDS44958.1 [P40616-1] |
RefSeqi | NP_001168.1, NM_001177.5 [P40616-1] |
Genome annotation databases
Ensembli | ENST00000261636.13; ENSP00000261636.8; ENSG00000120805.14 ENST00000536227.5; ENSP00000441808.1; ENSG00000120805.14 [P40616-2] ENST00000551828.5; ENSP00000448850.1; ENSG00000120805.14 [P40616-2] |
GeneIDi | 400 |
KEGGi | hsa:400 |
MANE-Selecti | ENST00000261636.13; ENSP00000261636.8; NM_001177.6; NP_001168.1 |
UCSCi | uc001tib.4, human [P40616-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28997 mRNA Translation: AAC37567.1 AF493887 mRNA Translation: AAM12601.1 BT007260 mRNA Translation: AAP35924.1 AK301701 mRNA Translation: BAG63173.1 AK311793 mRNA Translation: BAG34736.1 BX537387 mRNA Translation: CAD97629.1 AC063948 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97658.1 BC007000 mRNA Translation: AAH07000.1 |
CCDSi | CCDS44958.1 [P40616-1] |
RefSeqi | NP_001168.1, NM_001177.5 [P40616-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UPT | X-ray | 1.70 | A/C/E/G | 15-181 | [»] | |
4DCN | X-ray | 3.01 | A/B | 14-179 | [»] | |
5EE5 | X-ray | 2.28 | B | 15-181 | [»] | |
5J5C | X-ray | 3.40 | A | 16-181 | [»] | |
AlphaFoldDBi | P40616 | |||||
SMRi | P40616 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106893, 79 interactors |
CORUMi | P40616 |
IntActi | P40616, 36 interactors |
MINTi | P40616 |
STRINGi | 9606.ENSP00000261636 |
Chemistry databases
DrugBanki | DB04315, Guanosine-5'-Diphosphate |
PTM databases
iPTMneti | P40616 |
MetOSitei | P40616 |
PhosphoSitePlusi | P40616 |
SwissPalmi | P40616 |
Genetic variation databases
BioMutai | ARL1 |
DMDMi | 728888 |
Proteomic databases
EPDi | P40616 |
jPOSTi | P40616 |
MassIVEi | P40616 |
MaxQBi | P40616 |
PaxDbi | P40616 |
PeptideAtlasi | P40616 |
PRIDEi | P40616 |
ProteomicsDBi | 5387 55375 [P40616-1] |
TopDownProteomicsi | P40616-1 [P40616-1] |
Protocols and materials databases
Antibodypediai | 30383, 286 antibodies from 26 providers |
DNASUi | 400 |
Genome annotation databases
Ensembli | ENST00000261636.13; ENSP00000261636.8; ENSG00000120805.14 ENST00000536227.5; ENSP00000441808.1; ENSG00000120805.14 [P40616-2] ENST00000551828.5; ENSP00000448850.1; ENSG00000120805.14 [P40616-2] |
GeneIDi | 400 |
KEGGi | hsa:400 |
MANE-Selecti | ENST00000261636.13; ENSP00000261636.8; NM_001177.6; NP_001168.1 |
UCSCi | uc001tib.4, human [P40616-1] |
Organism-specific databases
CTDi | 400 |
DisGeNETi | 400 |
GeneCardsi | ARL1 |
HGNCi | HGNC:692, ARL1 |
HPAi | ENSG00000120805, Low tissue specificity |
MIMi | 603425, gene |
neXtProti | NX_P40616 |
OpenTargetsi | ENSG00000120805 |
PharmGKBi | PA24985 |
VEuPathDBi | HostDB:ENSG00000120805 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0072, Eukaryota |
GeneTreei | ENSGT00940000155118 |
HOGENOMi | CLU_040729_9_4_1 |
InParanoidi | P40616 |
OMAi | QEGMDWL |
OrthoDBi | 1271528at2759 |
PhylomeDBi | P40616 |
TreeFami | TF105461 |
Enzyme and pathway databases
PathwayCommonsi | P40616 |
Reactomei | R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network |
SignaLinki | P40616 |
Miscellaneous databases
BioGRID-ORCSi | 400, 96 hits in 1086 CRISPR screens |
ChiTaRSi | ARL1, human |
EvolutionaryTracei | P40616 |
GeneWikii | ARL1 |
GenomeRNAii | 400 |
Pharosi | P40616, Tbio |
PROi | PR:P40616 |
RNActi | P40616, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000120805, Expressed in islet of Langerhans and 252 other tissues |
ExpressionAtlasi | P40616, baseline and differential |
Genevisiblei | P40616, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR024156, Small_GTPase_ARF IPR006689, Small_GTPase_ARF/SAR |
PANTHERi | PTHR11711, PTHR11711, 1 hit |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SMARTi | View protein in SMART SM00178, SAR, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARL1_HUMAN | |
Accessioni | P40616Primary (citable) accession number: P40616 Secondary accession number(s): B4DWW1, P80417, Q53XB1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 207 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families